Journal of Applied Bioanalysis

Journal Information
EISSN : 2405-710X
Published by: Betasciencepress Publishing (10.17145)
Total articles ≅ 106
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Vijayakumar A, Sudha V, , Jeyakumar Sm,
Journal of Applied Bioanalysis, Volume 7;

OBJECTIVE: A high-performance liquid chromatography method for the estimation of Linezolid in human plasma was developed and validated. METHODS: Samples (100µµL) were deproteinized with acetonitrile and analyzed using LiChrospher 100, RP18e column with PDA detection at 254 nm. The flow rate of the isocratic mobile phase comprising of 0.1% formic acid in 1000 ml of water and acetonitrile in the ratio of 60:40 (v/v) was set at 1.0 ml/min. RESULTS: The calibration curve ranged from 0.50 to 20.0 µg/ml and was linear. The recovery ranged from 96% to 101%. The accuracy ranged from 98 to 101% and intra- and inter-day relative standard deviation was <4.58%. The method reliably eliminated interfering materials from plasma and R2 was 0.9973. The method described was applied to the determination of plasma LZD concentration in multi-drug-resistant tuberculosis patients who are treated with a dose of 600 mg LZD once daily. CONCLUSIONS: The developed method is suitable for determination of plasma LZD in routine care and considered feasible in less-resourced settings
Neville Ng, Reece Gately,
Journal of Applied Bioanalysis, Volume 7, pp 11-18;

Automated liquid handling stations such as the Hamilton Microlab range can be implemented to greatly enhance throughput of cell-based and cell-free assays. To facilitate utilisation past the comprehensive programming interface of the Hamilton Method Editor this paper presents a user interface (UI) that runs within Hamilton Venus and allows for a user to control and store programs for plate-to-plate transfers and serial dilutions in 96 well plate format. The interface allows for rapid control of aspiration and dispensing height, trituration, control of tip rack selection, and includes a tip washing program that can reduce the number of disposable tips utilised. The simple program interface allows the Hamilton Microlab to be used as a readily deployable microtitre plate handler, particularly for applications such as aliquoting cells for seeding, diluting a number of plates in medium, or transferring drug dilution arrays to multiple plates. This paper also discusses various optimisations to increase accuracy and rate of low volume liquid transfer. While complex liquid handling tasks such as high-throughput drug screening requires established core facilities, standalone liquid handlers with simplified interfaces can be utilised for smaller-scale research applications and educational purposes.
Xiaoshuang Yan, Chaochi Yeh,
Journal of Applied Bioanalysis, Volume 6, pp 107-130;

[icon name="unlock"] OPEN-ACCESS [icon name="eye"] PEER-REVIEWED REVIEW Xiaoshuang Yan, Chaochi Yeh, Linglong Zou* Bioanalytical Sciences, Shanghai Henlius Biotech, Shanghai, China.... Along with improved knowledge of cancer biology and biotechnical progress, diagnostic approaches have evolved from tissue biopsies to liquid biopsies.
, Omnia Ismaiel, James Waltrip, Tom Mariannino, Moucun Yuan, William Mylott, Vikram Roongta, Jim Shen, Pathanjali Kadiya
Journal of Applied Bioanalysis, Volume 6, pp 145-163;

[icon name="unlock"] OPEN-ACCESS [icon name="eye"] PEER-REVIEWED ORIGINAL RESEARCH Morse Faria1,*, Omnia A. Ismaiel1,2, James Waltrip1, Tom Mariannino1, Moucun Yuan1, William... This paper describes a validated method to simultaneously measure tanespimycin and its active metabolite in human plasma by LC-MS/MS.
, Heather E. Murrey, Kay Ahn, Naidong Weng, Shefali Patel
Journal of Applied Bioanalysis, Volume 6, pp 131-144;

[icon name="unlock"] OPEN-ACCESS [icon name="eye"] PEER-REVIEWED ORIGINAL RESEARCH Yifan Shi1,*, Heather E. Murrey2, Kay Ahn2, Naidong Weng1, Shefali Patel1 1DMPK,... A high-throughput LC-MS method for TXB2 and PGE2 was developed for human whole blood assay for COX inhibition.
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