Biogenesis: Jurnal Ilmiah Biologi

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ISSN / EISSN : 2302-1616 / 2580-2909
Total articles ≅ 194
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Melewanto Patabang, Hardjanto Hardjanto
Biogenesis: Jurnal Ilmiah Biologi, Volume 9, pp 137-145; https://doi.org/10.24252/bio.v9i2.22281

Abstract:
Pinus merkusii Jungh et de Vriese is a plant with high market and economic values due to providing a variety of products, including wood. Good management is needed to preserve pine forest products. The first step toward effective forest management is developing a management plan based on estimated stock data. The volume table used has a significant impact on the accuracy of the stock potential estimate. This study aims to determine the best equation model for compiling a local volume table for P. merkusii Jungh et de Vriese in Tana Toraja Regency. The research was conducted at the community pine forest in Gandang Batu Sillanan and Mengkendek districts, Toraja Regency. The number of sample measured was 100 trees. An analytical method was used to calculate the amount of tree volume on allometric equations. The equation for the stem volume was made using the allometric equation. The research results showed that diameters' mean, standard deviation, and sampling error values were 28.34 cm, 7.39 cm, and 1.45, respectively, heights were 23.77 m, 7.37 m, and 1.44; volume was 1.44 m3, 0.96 m3, and 0.19. The regression equation modelling showed that the best equation model for estimating volume P. merkusii Jungh et de Vriese based on height and diameter was 𝑉=𝑎𝐷2𝐻.
Aulia Noor Ramadhani, Diah Rachmawati
Biogenesis: Jurnal Ilmiah Biologi, Volume 9, pp 126-136; https://doi.org/10.24252/bio.v9i2.20754

Abstract:
Rice is an important staple food in Indonesia. Crop areas can be expanded to boost productivity by utilizing marginal lands, which are saline. This study aims to study the growth and physiological response of rice ‘Inpari 35’ to the application of silicate fertilizer under salinity stress conditions. This study used a completely randomized design (CRD) with two factors. The first factor is the difference in NaCl salt concentration consisting of N0: 0 mM; N1: 37.5 mM; N2: 50 mM, while the second factor is the difference in the concentration of silicate fertilizer (CaSiO3) consisting of S0: 0 mM; S1: 1 mM and S2: 2 mM. Each treatment combination was repeated three times. Observed data were analyzed by analysis of variance (ANOVA). A significant difference between treatments is continued with Duncan's multiple distance test at a 95% confidence level. The results showed that NaCl treatment significantly (p<0.05) inhibited the growth of rice ‘Inpari 35’, which was indicated by a decrease in the plant height and number of leaves. The NaCl treatment caused a reduction in the levels of chlorophyll, carotenoids, proline, membrane stability index (MSI), and relative water content (RWC). The interaction between NaCl treatment and CaSiO3 showed significant differences in physiological parameters by increasing the levels of chlorophyll, carotenoid, proline, membrane stability index, and relative water content.
Ipin Aripin, Topik Hidayat, Nuryani Y Rustaman, Riandi Riandi
Biogenesis: Jurnal Ilmiah Biologi, Volume 9, pp 156-162; https://doi.org/10.24252/bio.v9i2.23509

Abstract:
Mango cross-pollination can be encouraged through the presence of pollinating insects, which can be investigated and observed through citizen science activities. This study aims to monitor the presence of insect pollinators of mango (Mangifera indica L.) inflorescence through citizen science activities. The data generated in the study can be used as a reference to determine population trends and the biodiversity of mango insect pollinators. A citizen science approach in participatory research was used to collect and identify the data. A total of 68 volunteer participants from two universities in west Java were involved in this study. The participants had to meet the requirements to have contracted ecology courses. Smartphones and insect identification guidelines and databases at https://www.discoverlife.org/ and https://www.inaturalist.org/ were used as a tool in this research. The identified data were submitted via google form (www.bit.ly/csmangga) and the Inaturalist application for publication. It was discovered that mango inflorescence insect pollinators comprised five orders, 26 families, and 39 species. Diptera and Hymenoptera orders are insects that have the biggest role in mango pollination, and Chrysomya sp. is an insect species found in almost all mango cultivars.
Noor Laila Safitri, Erma Prihastanti, Sri Widodo Agung Suedy, Agus Subagio
Biogenesis: Jurnal Ilmiah Biologi, Volume 9, pp 163-170; https://doi.org/10.24252/bio.v9i2.23524

Abstract:
Chili pepper (Capsicum frutescens L.) is a horticulture product with a limited shelf life due to quality degradation following harvest. One method of preserving the quality of chili peppers can be evaluated by using nano-chitosan, which combine chitosan and sodium tripolyphosphate (STPP) in certain ratio. The purpose of this study was to determine the impact of nano-chitosan on maintaining the quality of C. frutescens L., the optimal ratio of chitosan to STPP for preserving the quality of C. frutescens L. after harvest, and the shelf life of C. frutescens L. treated with nano-chitosan after harvest. This study employed a completely randomized design (CRD) and included four treatments: P0 (control), P1 (0.2% nano-chitosan, 1:3 ratio), P2 (0.2% nano-chitosan, 1:4 ratio), and P3 (0.2% nano-chitosan, 1:5 ratio), which conducted for 16 days. Weight loss, water content, texture, color, and percentage of damage are the research variables in this study. The ANOVA test was used to examine the data, followed by the DMRT test. The results indicated that nano-chitosan could maintain the weight, water content, texture, and color of chili peppers. The optimal ratio of chitosan to STPP to retain the quality of postharvest C. Frutescens L. is 0.2% nano-chitosan (chitosan: STPP= 1:5), and nano-chitosan can maintain the quality of C. Frutescens L. for up to 16 days.
Nadhifa Athaya Khairunnisa, Dwi Umi Siswanti
Biogenesis: Jurnal Ilmiah Biologi, Volume 9, pp 146-155; https://doi.org/10.24252/bio.v9i2.21629

Abstract:
Abiotic stress is one of the elements that affect plant crop output due to its productivity and environmental factors. Salinity as abiotic stressors can impair plant growth, becoming a concern in the agriculture field in recent years. Biofertilizers are reported to be capable of overcoming salinity stress. Hence, it contains microbial elements in it, play a role for the nitrogen cycle in soil, which can result in increased plant growth. Therefore, this study aimed to analyze the effect of biofertilizer and NaCl as a salinity stress factor on the growth of Amaranthus tricolor L. The biofertilizer doses utilized in this study were 10 L/ha, 20 L/ha and 30 L /ha, each in combination with basic manure fertilizer. For the salinity factor, NaCl concentrations of 2500 ppm, 5000 ppm, 7500 ppm, and 10.000 ppm were employed. Environmental characteristics, plant height, number of leaves, root length, plant dry mass, chlorophyll and carotenoid content, as well as vitamin C, were all measured. The parameters were determined quantitatively. The chlorophyll and carotenoid contents were determined using a UV-vis spectrophotometer, while vitamin C levels were determined using iodometric titration. At a 95% level of confidence, the results were examined using the one-way ANOVA approach. The results indicate that a 30 L/ha dose of biofertilizer has an effect on the chlorophyll content and root length of plants, whereas a 10 L/ha dose has an effect on the carotenoid content. The highest amaranth growth was observed when 0 L/ha biofertilizer was combined with a 7500 ppm NaCl treatment, whereas the largest number of leaves was shown when 10 L/ha biofertilizer was combined with a 2500 ppm NaCl treatment. It could be concluded that while biofertilizer has no effect on plant growth parameters, it does increase plant productivity by raising chlorophyll and carotenoid levels.
Siti Lathifah Maulany, Ardyan Pramudya Kurniawan
Biogenesis: Jurnal Ilmiah Biologi, Volume 9, pp 189-197; https://doi.org/10.24252/bio.v9i2.24091

Abstract:
Karimunjawa National Park provides substantial reef fish resources, leading to higher market demand for reef fish production. This study aims to determine the diversity of reef fish, particularly in regions where fishing boats dock, specifically in Legon Bajak Port, Kemujan Island, Karimunjawa National Park, Jepara, Central Java. The findings of this investigation should enrich previous studies. The study was conducted on the right and left sides of the Legon Bajak Port. The visual count of belt transects was used to obtain data on reef fish. The study results obtained the number of reef fish at the Legon Bajak Port was 2769 individuals/500 m2 on the right and 1809 individuals/500 m2 on the left side, representing 40 species, 26 genera, and 13 families. Istigobius rigilius had the largest density of individuals, with 233 individuals/500 m2. The fish diversity index (H') value on the right side of the Legon Bajak Port was 3.24, which includes the high category, and on the left side was 2.89, which includes the medium category. The dominance index (D) was between 0.05 and 0.06 (low), while the uniformity index (E) was between 0.74 and 0.83 (unstable in a stable community).
Okky Shavira Riesty, Dwi Umi Siswanti
Biogenesis: Jurnal Ilmiah Biologi, Volume 9, pp 178-188; https://doi.org/10.24252/bio.v9i2.22232

Abstract:
Throughout history, agricultural sector in Indonesia has faced a shortage of land. As a result, we must take use of land that is still available, even if the conditions are unsuitable for plant growth, one of which is salinity-stressed land. Amaranth is a frequently cultivated plant in Indonesia (Amaranthus tricolor L.). This vegetable plant is commonly consumed as food due to its nutritional content and numerous health benefits. To cultivate amaranth on salinity-stressed land, additional nutrients are required to ensure that the plants continue to thrive. One of them is the provision of biofertilizers, a type of organic fertilizer that contains beneficial bacteria for plant growth. This study aimed to determinate the effect of biofertilizer application on the growth and diameter of the stem metaxylem of amaranth plants growing in a salinity-stressed environment. As a salinity stress treatment, NaCl was applied at doses of 0, 2500, 5000, 7500, and 10000 ppm. The diameter of the metaxylem was determined by making fresh preparations across amaranth stems. The biofertilizer application does not affect the height and number of leaves of the plant. However, as the dose of biofertilizer was increased, the value of the metaxylem diameter of the stem increased.
Zulheri Noer, Maimunah Maimunah, Erwin Pane, Eko Prasetya
Biogenesis: Jurnal Ilmiah Biologi, Volume 9, pp 198-205; https://doi.org/10.24252/bio.v9i2.24092

Abstract:
Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial leaf blight on rice plants, which is responsible for crop failure, resulting in economic losses. Control of Xoo bacteria can be accomplished through the use of Xoo-resistant rice varieties. Due to the shifting genetic structure and environmental conditions of Xoo bacteria, it is difficult to identify them according to their pathotype. This study aims to determine the genetic diversity of Xoo bacteria using the polymerase chain reaction (PCR) method and the IS1112-based RAPD marker, which is known to contain repetitive sequences. DNA was isolated from 52 isolates collected from 15 districts and cities in North Sumatra province and then used to amplify the IS1112 sequence. Dendrogram analysis revealed that cluster analysis of PCR findings classified isolates into 15 groups with a 90% similarity value. Genetic variation among Xoo isolates from North Sumatra ranged between 51% and 100%. North Sumatra Xoo isolates exhibited a high degree of genetic diversity. This findings can be used as a resource for future management of the Xoo bacteria by expediting disease-resistant rice breeding projects in various rice producing countries.
Dzul Fithria Mumtazah, Hendri Busman, Mohammad Kanedi, Gina Dania Pratami
Biogenesis: Jurnal Ilmiah Biologi, Volume 9, pp 171-177; https://doi.org/10.24252/bio.v9i2.24081

Abstract:
Researchers attempted to obtain animal models that experienced hypercholesterolemia and led to liver damage to determine the performance of the liver. Rats were chosen because of the ease of handling, collecting organ and blood samples. Still, their high price and availability that are more difficult to find have made some researchers look for alternatives to other animal models such as mice. This study aims to determine the role of a modified high-fat diet as an alternative diet for mice to experience hepar damage. Male mice treated for four and eight weeks with a modified high-fat diet were sacrificed for their liver, then they were tested for histopathology using the paraffin method and HE staining. The characterization of hepar damage traits was carried out to score the degree of parenchymal degeneration, hydropic degeneration, and necrosis. The results showed that the control group, four and eight weeks of atherogenic diet had more than 50% cell damage, presumably due to the role of the starch mixture in feed as a source of carbohydrates through the mechanism of converting carbohydrate pathways into fat which damages liver cells.
Budi Setiadi Daryono, Puti Hana Ramadhani, Esty Nidianti
Biogenesis: Jurnal Ilmiah Biologi, Volume 9, pp 118-125; https://doi.org/10.24252/bio.v9i2.15997

Abstract:
Cultivar ‘Kinaya’ is a melon produced from breeding between ‘Sonya’ and ‘Kinanti’. This study on phenotypic and molecular characters is carried out to support ‘Kinaya’ as a superior cultivar. Our study aimed to analyze the genetic variation of the melon ‘Kinaya Bulat Kuning’ and ‘Kinaya Kuning Lonjong’ and its parental ‘Sonya’ and ‘Kinanti’. The research was conducted in the Greenhouse of Mutihan, D.I.Yogyakarta, and the Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada. Quantitative characters were determined by morphometric comparisons, fruit weights, and the number of seeds. Qualitative characters included fruit color, fruit skin color, aroma, texture, and taste. The molecular characterization method was inter simple sequence repeat (ISSR) and included DNA isolation, spectrophotometry, amplification of DNA target using PCR, and visualization of DNA target. Molecular characters were analyzed using spectrophotometry and visualization of DNA bands by electrophoresis using the MVSP 3.1 program. PCR used four random primers such as UBC 807, 809, 810, and 812, which obtained 11 polymorphic and 12 monomorphic DNA bands with a polymorphism rate of 47.8%. It is known that the cultivar ‘Kinaya’ has a similarity of 72% with its inductees ‘Sonya’ and ‘Kinanti’.
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