Biogenesis: Jurnal Ilmiah Biologi

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ISSN / EISSN : 23021616 / 25802909
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Farah Nadia Karima, Mulyati Sarto
Biogenesis: Jurnal Ilmiah Biologi, Volume 7; doi:10.24252/bio.v7i1.7292

Abstract:Stress is a psychiatric/psychological disorder characterized by a sense of disability, despair, anhedonia, decreased activity, and pessimism. Stress can affect the physiological condition of the body with symptoms of lipid metabolism disorders. Chlorella vulgaris is a microalgae that is known to have the potential as an alternative antidepressant drug. The study was conducted to determine the lipid profile of blood wistar strain rats after stress induction and the effect of administration of Chlorella vulgaris on blood lipid profiles of stress-induced wistar rats. This research is an experimental study using 5 treatments with 25 models of Rattus norvegicus wistar strain, 2 months old. The treatment group consisted of 1 control group, 1 stress group, and 3 groups of treatment variations, namely the treatment of antidepressant medication, the treatment of administration of cultivated Chlorella vulgaris, and the treatment of commercial Chlorella. Stress induction is carried out by treatments that given to the rats randomly, namely cold water, warm water, wet cage, dark-light cycle, and sound wave exposure for 40 days. Examination of blood lipid profiles was carried out on Day 0 after mice were acclimated, day 40 after rats were induced stress, and day 56 after rats were given treatment of cultivated Chlorella vulgaris. The results obtained were the stress conditions of the lipid profile of the wistar strain of rats which had increased were total cholesterol levels of the control group and triglyceride levels in all groups while those who had decreased were cholesterol levels other than the control group, HDL levels in all groups, and LDL levels all groups. Giving Chlorella vulgaris had effect on decreasing total cholesterol levels, triglyceride levels, and LDL levels along with increasing HDL levels.
Suparti Suparti, Lina Agustina, Putri Agustina, Resti Rahmawati
Biogenesis: Jurnal Ilmiah Biologi, Volume 7; doi:10.24252/bio.v7i1.6102

Abstract:Potato Dextrose Agar (PDA) is the most media to grow the mushrooms, but the price of this media is expensive. Thus we need alternative media that easy and cheap to get it. This study aims to determine the growth of Aspergillus niger in alternative Artocarpus communis and Artocarpus heterophyllus seeds media. This research was an experimental study using a completely randomized design (CRD) one factor was the type of media is PDA (M0), Artocarpus communis seed media (M1), Artocarpus heterophyllus seed media (M2) and using the test mushrooms A. niger (J1). Inoculation of A. niger used agar block method for 3 days with a temperature of 28°C. Parameter of research was colony diameter and sporulation of A. niger. Data obtained with qualitative and quantitative methods. The result of this research showed that the best growth for Aspergillus niger was after 72 hours incubation. Colony diameter continually in PDA media, Artocarpus communis media, and Artocarpus heterophyllus media is 4.7 cm, 4.3 cm, and 4.1 cm with heavy sporulation. Therefore, Artocarpus communis and Artocarpus heterophyllus seeds media can be utilized as a substitution of PDA media for the growth of mushrooms.
Agus Nuryanto, Rani Eva Dewi, Hendro Pramono
Biogenesis: Jurnal Ilmiah Biologi, Volume 7; doi:10.24252/bio.v7i1.6352

Abstract:Commerson’s anchovy (Stolephorus commersonnii) is a small pelagic fish that live in a group and its existence is very abundant in Segara Anakan Cilacap. This anchovy is widely consumed by communities live around Segara Anakan. This leads to a high exploitation rate. Exploited populations generally have low genetic diversity. This study aims to evaluate genetic diversity of commerson’s anchovy population in Segara Anakan Cilacap inferred from PCR-RFLP of the cytochrome c oxidase 1 (CO1) gene. This study was conducted from January to April 2018 and used survey method by applying random sampling. As many as 30 samples of anchovy were taken. Genomic mtDNA was isolated using modified Chelex method. Partial sequences of the COI gene were amplified using a pair forward commercially available primer. The lengths of 650 base pair of the PCR products were digested with four restriction enzymes. The HindIII enzyme produces PCR-RFLP fragment with the size of 416 bp and 234 bp lengths, VspI produces 435 bp and 214 bp, CO1-TaqI produces 556 bp and 94 bp and RsaI produces 319 bp, 183 bp, and 148 bp fragments, respectively. The PCR-RFLP fragments were obtained from all samples but they produced uniform band pattern for all 30 anchovy individuals. These results indicated that the anchovy population in Segara Anakan Cilacap has monomorphic allele for all PCR-RFLP markers. Hence, it can be concluded that genetic homogenity was observed on anchovy population in Segara Anakan Cilacap as inferred from PCR-RFLP COI gene.
Angga Prasetyo, Boy Rahardjo Sidharta, Yustina Sri Hartini, Exsyupransia Mursyanti
Biogenesis: Jurnal Ilmiah Biologi, Volume 7; doi:10.24252/bio.v7i1.6000

Abstract:Red ginger (Zingiber officinale var. rubrum) has been proven to show anticancer activity. Direct use bioactive compound from red ginger has many obstacles such as large amount of red ginger’s rhizome needed, limitation of planting area, and very long time of harvesting. Utilization of endophytic fungi from red ginger’s rhizome could be an alternative to the problems. The aims of this study were to determine bioactive compound produced by endophytic fungi and toxicity activity based on LC50. Endophytic fungi were isolated from red ginger and were identified macroscopically and microscopically. The bioactive compounds were extracted using ethanol 96%. Flavonoid test was done qualitatively, bioactive compounds were analyzed by Thin Layer Chromatography (TLC), and the toxicity test was done using Brine Shrimp Lethality Assay (BSLA). The present research found two endophytic fungi isolated from red ginger rhizome. Isolate 1 was similar to Mucor sp. and isolate 2 was similar to Trichoderma sp. Phytochemical test revealed bioactive compound extracted from the isolates were contained flavonoid. TLC analysis did not detect quercetin from the bioactive compound extracted from the isolates. LC50 values of the bioactive compound from the isolates were 2.300 and 1.747 µg/ml, respectively. The toxicological results suggest that both isolates produce non-toxic compound to Artemia salina.
Rich Gemilang Simanjuntak, Rury Eprilurahman
Biogenesis: Jurnal Ilmiah Biologi, Volume 7; doi:10.24252/bio.v7i1.7803

Abstract:The shape of chelae and carapace can be used to distinguish between species of prawn. This study aims to determine the variations in the shape of chelae and carapace in several species belonging to the genus Macrobrachium using analysis of geometric morphometric. This study uses photos of specimens that have been processed with several TPS software. Data analyzed statistically by PCA using the MorphoJ software. Clustering analysis using UPGMA method using PAST software. The results showed the carapace shape grid deformation varied at the tip of the rostrum, the tip of the ocular spine and the lower curvature of the front of the carapace, and the base spines of rostrum. Grid deformation in the shape of chelae varies at the tip of the pollex, the junction between the pollex and the manus on the inferior margin of the propodus, the upper and lower points marking the junction of the dactylus with the propodus. PCA shows the total variation of the carapace shape is 82.66% which is divided into PC1: 75.11% and PC2: 7.55%. While the total variation of the shape of chelae is 87.56% which is divided into PC1: 55.49% and PC2: 32.07%. Clustering analysis shows the grouping of populations of Macrobrachium, the first group is M. latidactylus and M. sintangense, the second group includes M. horstii and M. latimanus. M. lar is a species that shows the similarity of the shape of the carapace and chelae with the two groups. M. rosenbergii and M. pilimanus are on different lines.
Wiwik Kusmawati, Lia Rahayu
Biogenesis: Jurnal Ilmiah Biologi, Volume 7; doi:10.24252/bio.v7i1.5786

Abstract:Data from the Malang City Health Office shows the increasing number of refill drinking water depots in the city of Malang until 2013 there were 163 drinking water depots scattered in each Malang Subdistrict. The purpose of this study was to determine the contamination of Escherichia coli refill drinking water in drinking water depots in Malang. The samples taken were 20 refill drinking water depots in Malang. Sampling is done by simple random sampling. Escherichia coli examination using the Most Probable Number Test. Of the 20 samples tested, 2 samples met the requirements of drinking water quality according to PERMENKES and 18 other samples containing the number of coliform bacteria exceeding the maximum limit of 0 per 100 ml of water. There were Escherichia coli in 10 samples while 8 other samples contained other coliform bacteria.
Made Deviani Duaja, Elis Kartika, Lizawati Lizawati
Biogenesis: Jurnal Ilmiah Biologi, Volume 7; doi:10.24252/bio.v7i1.5990

Abstract:Opencast mining caused heavy deforestation and barren land in Jambi. This ex-mining land must be reclaimed by planting crops based on biofertilizers containing mycorrhizae, this is a potential alternative that provides benefit both agronomy plant and ecosystem specially replanting with oil palm. Those are the technology for reclamation ex-coal mining soil. This research aim is to examine the effect of four combinations indigenous isolate of Arbuscular Mycorrhizae Fungi (AMF), which is originated from ex-coal mining soil that combined with Phosphorous fertilizers to minimize utilizing P fertilizer at oil palm pre-nursery, to improve soil fertility, and seedling growth. Hence, this research was arranged in factorial experiment with 2 factors, using a complete randomized design with three replications. The first factor is five different mycorrhizae isolates, representing a broad range of endomycorrhizae fungi, there are: without indigenous AMF, isolate of Glomus sp. 3, isolates of Glomus sp. 6, isolates of Glomus sp. 15 and isolates of Glomus sp. 16. The second factor is P fertilizer: control (without P fertilizers), fertilizer dosage P 25%, 50%, 75%, and 100%. Variables measured are shoot height, number of leaves per plant, total leaf area per plant, and plant stem diameter. The data were analyzed by analysis of variance to see whether there was an interaction between the MVA isolate and the P fertilizer dosage. The result showed a significant effect on palm oil seedling growth, shoot height, number of leaves per plant, total leaf area per plant but there is no interaction on the stem diameter. Plants that are inoculated with mycorrhizae have greater P content in leaves than those not inoculated. The types of isolated Glomus sp.3 and dosage P 75% of recommended dosage, give the best seedling growth. This research concluded that inoculation with AMF could minimize P fertilizers doses.
Ganies Riza Aristya, Rezika Alyza
Biogenesis: Jurnal Ilmiah Biologi, Volume 7; doi:10.24252/bio.v7i1.5250

Abstract:Strawberry is fruit commodities which originating from the member of Rosaceae and has been widely cultivated in several countries of the world including Indonesia. One of the strawberry cultivars which consumed and cultivate in Indonesia is the strawberry Festival (Fragaria x ananassa D. var. Festival), which was developed in the area of Banyuroto, Magelang. In order to improve the quality and quantity of strawberry plants, polyploidization using colchicine has been employed in previous studies. The objectives of this research were study the chromosome character which include number, size, shape, and karyotype of normal strawberries and treatment strawberries with colchicine (0.05% 36 hours of root and leaf induction, 0.05% 24 hours of root and leaf induction, 0.05% 36 hours of leaf induction, 0.01% 36 hours of root and leaf induction). The results of this research shown that chromosome number difference and degree of ploidy between Fragaria x ananassa var Chandler in America with Fragaria x ananassa var. Festival in Indonesia due to natural mutation. While Festival strawberry control and treatment colchicine have same number chromosome is 2n=4x=28M and there are a difference long sleeve and short sleeve chromosome that may affect the content of chromosome expression and phenotype character are produced. The analysis of flow cytometry showed that treatment strawberry with colchicine has two peaks with intensity fluorescence equal to strawberry control so that it can be interpreted that the ploidy number of strawberries treatment with colchicine is tetraploid.