Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology

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ISSN / EISSN : 2089-5690 / 2406-9272
Total articles ≅ 264
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Wan Norhana Md Noordin, Nannthini Shunmugam, ,
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 16; doi:10.15578/squalen.448

Abstract:
The objectives of this study were to determine the effects of garlic oil (GO) and tartaric acid (TA) on microbiological, pH, and Thiobarbituric Acid Reactive Substances (TBARS) (lipid peroxidation) of shrimp stored at 4°C. Shrimp of 2 kg were dipped in GO and TA solutions at 1:2 shrimp/treatment solutions (w/w) for 30 mins under 25ºC. Sodium metabisulfite (MBS) and sterile distilled water (dH20) were used as positive and negative controls, respectively. Shrimp were drip-dried for 5 mins, packaged, and stored in a chiller (4°C) for 10 days. The shrimp were analysed on days 0, 3, 5, 7, and 10. Total aerobic plate count, psychotropic bacteria count, Listeria monocytogenes, Salmonella enterica serovar Typhimurium (S. Typhimurium) and Vibrio parahaemolyticus counts ranged from 3.52-8.73 log CFU/g,
Shanti Dwita Lestari, Rinto Rinto, Indah S Wahyuni, Sherly Ridhowati, Wulandari Wulandari
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 16; doi:10.15578/squalen.497

Abstract:
Fermented fish, including rusip and bekasam, contains lactic acid bacteria, some of which have probiotic properties. Considering the beneficial effect, the count of viable cells in probiotic carrier foods must be at least 106 CFU/g throughout the product shelf-life. The processing and storage condition significantly affect the viability of probiotics in carrier foods. This research was conducted to determine the heat resistance of probiotic candidate Enterococcus faecalis R22B isolated from rusip in three different media: bekasam, rusip, and physiological saline solution (NaCl 0.85%). E. faecalis R22B was heated in each medium at a temperature of 70 °C for 0, 2, 4, 8, and 12 min. The media characteristics showed differences within the parameters included pH values of 4.41-6.99; aw 0.67-0.92; moisture content 72.56-98.09%; ash content 0.69-12.32%; protein content 0-14.7%; fat content 0-0.84%; and carbohydrate content from 0-4.93%. The results showed that the difference in the heating medium had no significant effect (p>0.05) on the heat resistance of the bacteria, as indicated by the D70 value. The required average heating time at 70 oC to decrease E. faecalis R22B cells by 1log10 (D70 value) in bekasam, rusip, and physiological saline media were 3.17, 3.29, and 3.55 min, respectively. Despite having a lower DT-value, the reduction of bacteria cells in three media heated at 70 oC showed no difference (p>0.05); therefore, bekasam and rusip are suitable for probiotic carrier food.
Wan Norhana Md Noordin, Nannthini Shunmugam, ,
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 16; doi:10.15578/squalen.v0i0.448

Abstract:
The objectives of this study were to determine the effects of garlic oil (GO) and tartaric acid (TA) on microbiological, pH, and Thiobarbituric Acid Reactive Substances (TBARS) (lipid peroxidation) of shrimp stored at 4°C. Shrimp of 2 kg were dipped in GO and TA solutions at 1:2 shrimp/treatment solutions (w/w) for 30 mins under 25ºC. Sodium metabisulfite (MBS) and sterile distilled water (dH20) were used as positive and negative controls, respectively. Shrimp were drip-dried for 5 mins, packaged, and stored in a chiller (4°C) for 10 days. The shrimp were analysed on days 0, 3, 5, 7, and 10. Total aerobic plate count, psychotropic bacteria count, Listeria monocytogenes, Salmonella enterica serovar Typhimurium (S. Typhimurium) and Vibrio parahaemolyticus counts ranged from 3.52-8.73 log CFU/g,
, , Murshida Khan, Taslima Akter, Chunhong Yuan,
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 16; doi:10.15578/squalen.504

Abstract:
A natural product such as chitosan promotes health benefits and extends the shelf life of the processed products. Chitosan has been used as a food additive due to its antioxidant and antimicrobial activities. This study was conducted to optimize the chitosan concentration as a coating agent on the quality and shelf life of Labeo rohita fillets during frozen storage. Chitosan (CH) solutions were prepared with 0.5 %, 1 %, and 2 % (w/v) CH in 1 % (v/v) glacial acetic acid for coating application. The control (0% CH) and CH treated fish fillets were stored at -18 °C for 14 weeks and assessed for chemical (pH, thiobarbituric acid reactive substances (TBARS), total volatile basic nitrogen (TVB-N), and K-value), microbiological (aerobic plate count (APC)), and sensory properties. The pH, TBARS, and K-value of 1 % and 2 % CH treated fish fillets were acceptable up to 14th week of storage, while TVB-N value was permissible up to 12th week of storage. After 14th week of storage, the APC of control, 0.5 %, 1 %, and 2 % CH treated fillets were 7.18 log CFU/g, 5.02 log CFU/g, 4.13 log CFU/g, and 3.21 log CFU/g, respectively. The fish fillets treated with 1 % and 2 % CH had acceptable sensory attributes up to 12th week, while control fillets were unacceptable after the 6th week of storage. This study demonstrated that 1% CH could be used as a natural additive for keeping the quality and extending the shelf life of L. rohita fillets during frozen storage.
Bulletin Squalen
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 15; doi:10.15578/squalen.v15i3.521

Bulletin Squalen
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 15; doi:10.15578/squalen.v15i3.520

Squalen Bulletin
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 15; doi:10.15578/squalen.v15i3.522

Abdul Karim, Masudur Rahman, Esmout Jahan Alice, Amanullah, Akhtar Hossain, Tariqul Islam
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 15, pp 99-107; doi:10.15578/squalen.v15i3.483

Abstract:
In order to develop a proper packaging system for sliced tilapia fish (Oreochromis niloticus), the biochemical and microbiological qualities under control (unsealed package), vacuum package and modified atmosphere packaging with 50% CO2/50% N2 (MAP-1) and 50% CO2/50% O2 (MAP-2) were evaluated every three days during 18 days of chilled storage (4±1°C). The pH value was not significantly different (p > 0.05) by treatments until day 9, but significantly lower values (p < 0.05) were observed on day 12 of the storage in all treated samples compared to the control. The total volatile base nitrogen (TVB-N) value progressively increased, but not significantly different (p > 0.05) in all treatments during the entire storage period. The thiobarbituric acid reactive substances (TBARS) amounts were significantly lower (p < 0.05) on day 12 in the vacuum and MAP-1 samples compared to the control sample, and were significantly higher (p < 0.05) on day 6, 9, and 12 of the storage period in MAP-2 samples compared to the control, vacuum and MAP-1 samples. The amounts of pH, TVB-N, and TBARS in all samples did not exceed the acceptable limit in almost the entire storage. The total viable count (TVC) progressively increased with storage time. Nevertheless, TVC values were lower (p < 0.05) on day 6, 9, and 12 of the storage periods in all treatments compared to the control. The TVCs exceeded the acceptable limit (7 log CFU/g) on days 6-9 for control, 9-12 for vacuum, day 12 for MAP-2, and 15 for MAP-1 sample during the storage period. Therefore, the MAP has shown promising results for shelf life extension that can be practiced to display the fishery products with prolonged shelf life.
Msi Ir. Yusro Nuri Fawzya, Nugrah Analiadi Putra, Arif Budi Witarto, Gintung Patantis
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 15, pp 119-129; doi:10.15578/squalen.v15i3.511

Abstract:
Golden sea cucumber or locally known as “teripang emas” is one of Indonesia’s most popular sea cucumber and widely processed as functional food or supplement due to its bioactivities. The sea cucumber is often misidentified due to its morphological similarities with other Stichopus spp. This study aimed to identify the golden sea cucumber obtained from West Nusa Tenggara, Indonesia, by a molecular method and study the antioxidant activities of its collagen hydrolysates. The hydrolysates were produced by hydrolyzing acid collagen extract using neutrase for 30, 60, 120, 180, and 240 mins. The products were then analyzed for their degree of hydrolysis, peptide content, molecular weight distribution and radical scavenging activity by the 2,2-Diphenyl-1-picrylhydrazyl (DPPH) method. Results showed that hydrolysis for 180 mins was optimal in producing the highest peptide content, 12.79 ± 0.44 mg/mL, with a degree of hydrolysis (DH) of 55.2 ± 1.50%. However, the highest antioxidant activity (IC50 of 5.25 ± 0.15 mg/mL) was demonstrated after 60 mins hydrolysis with molecular weight (MW) ranged from less than 14.4 kDa to approximately 25 kDa. The hydrolysate might be categorized as a weak to moderate antioxidant. Based on the molecular identification, the golden sea cucumber had 99% similarities with Stichopus horrens and S. monotuberculatus.
Arif Rahman Hakim, Wahyu Tri Handoyo, Adrianto Widi Prasetya
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 15, pp 141-152; doi:10.15578/squalen.v15i3.454

Abstract:
Direct sunlight is commonly used to dry fresh seaweed by artisanal farmers in Indonesia due to its low cost and ease of handling. Nevertheless, this method poses some drawbacks such as lengthy duration, weather dependency and quality degradation. The application of microwave technology in food processing has progressed dramatically, including in the drying process. The microwave drying method is more efficient and can shorten the processing time. This study aimed to describe a large-scale microwave dryer (MD) design and performance to assist the fresh seaweed drying process. The design concept applies microwave energy with a volumetric heating feature to accelerate the seaweed drying process without damaging its functional groups. The MD dimensions were 2410 (l) x 270 (w) x 210 (h) mm with a dryer capacity of up to six kilograms, an enlarged cavity and multiple magnetrons. The main components of the MD were cavity, air circulation system, drying system and control system. According to the performance testing, the MD’s optimum performance was at a power level setting of P7 and six kilograms load. At this setting, we obtained a dried seaweed with a moisture ratio of 0.68±0.05, drying rate of 30.29±1.32 g/min, specific energy consumption of 3.96±0.08 MJ/kg H2O and energy efficiency of 58.45±2.65%. The total power of the P7 setting operation required 2.00 kW. Fourier-Transform Infrared (FTIR) spectra showed that the functional groups of the dried seaweed were unaltered.
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