Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology

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ISSN / EISSN : 20895690 / 24069272
Total articles ≅ 234
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Dwiyitno Dwiyitno
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 14, pp 141-153; doi:10.15578/squalen.v14i3.399

Abstract:Emerging contaminants (ECs) or contaminants of emerging concern (CECs) has become global awareness of researcher since few decades ago, including Indonesia. Intensive usage of industrial compounds has led to the massive emission to the environment and therefore their potential adverse effect may endanger aquatic organism and human health. Based on the available literatures, organotins and flame retardants as two main groups of ECs from industrial emission identified in Indonesian seafood, as well as worldwide. However, concentration both ECs group detected in Indonesian seafood are relatively low than that in developed countries and the majority of South East Asian countries. Aryl hydrocarbons are reported by only minor literatures in Indonesia and Japan suggested minor interest on the investigation to this compound group. Toxicological studies revealed that ECs emitted from industrial activities has to be concern, ash most of the ECs attributed to endocrine disrupting chemicals. Nevertheless, study on the exposure assessment of ECs in Indonesia is very limited. Regulation issue and laboratory approach are among future focus to be concern in addressing ECs in Indonesia, especially from industrial emission.
Jamal Basmal, Muhamad Luthfian Henrida, Rinta Kusumawati, Nurhayati Nurhayati
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 14, pp 131-139; doi:10.15578/squalen.v14i3.385

Abstract:The solid waste from agar processing industries in Indonesia is enormous. The waste contains numbers of macro and micro nutrient including growth hormone that can be a good source for plant fertilizer. Utilization of solid waste obtained from agar processing into fertilizer has been carried out by mixing with Sargassum powder, fish silage and Eucheuma cottonii pasta. Various concentrations of the solid waste (13, 19, 22 and 25%) and the Sargassum powder (0, 3, 6, 9, and 12%) were used in this study to obtain optimum nutrients in the formulated fertilizers i.e. growth hormone, total nitrogen and potassium. Growth hormone were analyzed using high-performance liquid chromatography (HPLC), while N content by Kjeldahl methods and K content analyzed according AOAC.The results showed that the optimum formulation was obtained from the combination of 25% solid waste and 0% Sargassum powder, which contained the highest growth hormones i.e. auxins (IAA) 46.75 ppm, gibberellin (GA3) 22.25 ppm, cytokinin-zeatin 18.50 ppm, and cytokinin-kinetin 61.00 ppm. While the potassium content was 0.43±0.001 ppm and the total nitrogen was 0.53± 0.06%. This result meets the requirements of the Minister of Agriculture Regulation No. 261/KPTS/SR.310/M/4/2019 regarding the requirements for total N in organic fertilizer. However, the total K+ does not meet the requirement. Therefore, enrichment with other organic raw material is still needed.
Squalen Bulletin
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 14; doi:10.15578/squalen.v14i3.421

Nurrahmi Dewi Fajarningsih, Naomi Intaqta, Danar Praseptiangga, Choiroel Anam
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 14, pp 103-111; doi:10.15578/squalen.v14i3.400

Abstract:Extraction and partial characterization of lectin from Indonesian Padina australis and Padina minor had been carried out. The crude extract of the P. australis and P. minor were examined for hemagglutination activity (HA) using native and trypsin-treated of rabbit and human A, B, O type erythrocytes. Both extracts agglutinated all of the trypsin-treated erythrocytes tested in the HA assay. Strong HA was detected in the crude extract of P. minor with trypsin-treated of human type A and O erythrocytes. However, the sugar-binding specificity study through the quantitative hemagglutination inhibition (HI) assay showed that P. minor extract could not specifically recognize the glycans tested. Apparently, the HA of the P. minor was more due to its co-extracted polyphenols content than its lectin content. On the other hand, the HI assay showed that asialo transferrin human (aTf) and asialo porcine thyroglobulin (aPTG) were the most powerful in inhibiting the HA of P. australis. Those indicated that P. australis protein extract was able to specifically recognized aTf and aPTG. The stability of P. australis and P. minor HA over various temperatures, pH ranges, and divalent cations studies showed that the P. minor HA was stable on a wide range of pH and temperature; not affected by the presence of EDTA, but decreased by Ca2+ and Mg2+ additions showed that P. minor protein extract was not a metallic protein. The HA of P. australis decreased at 60 oC and was inactivated at 90 oC; increased at strong acidic (pH 3 & 4) and strong basic (pH 9 & 10) and dependent by the presence of either EDTA or Ca2+ and Mg2+ divalent cation.
Akhmad Awaludin Agustiar, Imas Faturrohmah, Bekti Wulan Sari, Nurul Binti Isnaini, Indun Dewi Puspita, Triyanto Triyanto, Amir Husni, Ustadi Ustadi
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 14, pp 113-120; doi:10.15578/squalen.v14i3.414

Abstract:Chitin hydrolysate is one of the value added product derived from shrimp shell waste. Production of chitin hydrolysate using biological process offers an environmental friendly method compared to chemical process. Serratia marcescens PT-6, a gram negative chitinolytic bacterium isolated from shrimp pond sediment, shows good activity in hydrolyzing chitin. This study aimed to improve the chitinase activity of S. marcescens PT-6 culture by optimizing the component of chitin-containing medium (additional nitrogen source, additional carbon source, and colloidal chitin). The optimization of chitinase by S. marcescens PT-6 culture was done using one variable at a time method. The sequence of the research were to optimize 1) the type of additional carbon source (glucose, lactose, sucrose, and starch), 2) the type of additional nitrogen source (yeast extract, peptone, ammonium sulphate, and ammonium chloride), 3) the concentration of colloidal chitin (0.5; 1; 1.5; 2; and 2.5%), and 4) the concentration of the additional carbon and nitrogen source. The culture of S. marcescens PT-6 was incubated in colloidal chitin medium at 30 oC and chitinase activity from culture supernatant was analyzed. The results showed that starch gave the highest chitinase activity compare to other carbon source, meanwhile yeast extract was chosen as the best nitrogen source among others. The combination of 1.5% colloidal chitin with 0.5% starch and 0.1% yeast extract in medium increased the chitinase activity of S. marcescens PT-6 to 0.021 U/ml. These results indicated that an appropriate medium composition could increase the chitinase activity produced by S. marcescens PT-6 culture.
Eti Kurniawati, Bustami Ibrahim, Desniar Desniar
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 14, pp 121-130; doi:10.15578/squalen.v14i3.404

Abstract:Fish protein hydrolysate (FPH) is a derivative product of fish proteins containing smaller peptides and amino acids. FPH products have high water solubility, good emulsion capacity, and large expanding ability. With its functional properties, it allows FPH to be used as a raw material in the manufacturing of secondary microbiological reference materials. This study was intended to characterize catfish (Clarias sp.) FPH as a candidate for the matrix of microbial secondary reference. The FPH was prepared through enzymatic hydrolysis, freeze-drying and milling. The hydrolysis processes were carried out using 5% (w/w) papain, 55 °C for 5 hours, then the papain activity was stopped by increasing the temperature to 80 °C for 20 minutes.The FPH was combined with gelatine, sodium glutamate, glucose solution, and was spiked with Salmonella enteritica sv Enteritidis and freeze-dried. Results showed that catfish FPH was yellowish-white powder with a FPH yield of 11.05%. The proximate analysis of FPH revealed the moisture content of 3.77 ± 0.12%, ash content of 7.26 ± 0.03%, protein content of 86.09 ± 0.17%, and fat content of 1.38 ± 0.07%. The protein content of the FPH was greater than skim milk (33.42%). Carbohydrate levels of catfish FPH and skim milk were 1.56% and 57.46%, respectively. The best concentration of catfish FPH to perform as a microbiological reference material was 14%, obtained from highest viability of Salmonella bacteria and homogeny. The candidate for reference material were stable at storage temperatures of -20 oC.
Squalen Bulletin
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 14; doi:10.15578/squalen.v14i3.422

Squalen Bulletin
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 14; doi:10.15578/squalen.v14i3.420

Squalen Bulletin
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 14; doi:10.15578/squalen.v14i2.403

Fateha Fateha, Singgih Wibowo, Joko Santoso, Agusman Agusman, Uju Uju
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 14, pp 65-72; doi:10.15578/squalen.v14i2.397

Abstract:Alkali treated cottonii (ATC) is a derivative product of Eucheuma cottoniithat is treated with alkali. This study used raw material of sap-free seaweed for ATC processing. Sap-free seaweed is a seaweed which thallus has been extracted with liquid known as sap. The use of sap-free seaweed as an ATC product is constrained by its low quality, so an effort to improve its quality is needed. Therefore the obejcetive of this Research was to get the optimal conditions of ATC processing. Experimental data were designed and analyzed using Response Surface MethodologyCentral Composite Design (RSM-CCD) using Design Expert 10.0.7® program. The optimization of ATC processing involved three components that were considered influential, namely KOH concentration, temperature, and processing time. Recommendation for optimal conditions issued by RSM-CCD on ATC processing from sap-free seawee d were 6 % KOH concentration at 75 oC for 120 minutes (93.1% desirability). The results of the response analysis showed a yield of 39.47% and a gel strength of 595.32 g/cm2. As a research control, the recommendation of RSMCCD used in ATC processing from nonsap-free seaweed was obtained yield of 36.81% and gel strength of 574.44 g/cm 2. ATC from sap-free seaweed has higher yield and gel strength than that from nonsap-free seaweed. Thismight be due to the sap-free seaweed was obtained using the proper sap extraction process and ATC processingunder optimal conditions, so that the quality of seaweed was better maintained.