Scientific Journal of Microbiology

Journal Information
EISSN : 2322-2948
Published by: Academic World Research (10.14196)
Total articles ≅ 7
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Tekalign Tadesse, Amanuel Bekuma
Published: 30 July 2017
Scientific Journal of Microbiology, Volume 6, pp 162-171; https://doi.org/10.14196/sjm.v6i7.2558

Abstract:
Camelpox is an economically important, notifiable skin disease of camelids and could be used as a potential bio-warfare agent. The disease is caused by the camelpox virus, which belongs to the Orthopoxvirus genus of the Poxviridae family. Young calves and pregnant females are more susceptible. Tentative diagnosis of camelpox can be made based on clinical signs and pox lesion, but it may confuse with other viral diseases like contagious etyma and papillomatosis. Hence, specific, sensitive, rapid and cost-effective diagnostic techniques would be useful in identification, thereby early implementations of therapeutic and preventives measures to curb these diseases prevalence. Treatment is often directed to minimizing secondary infections by topical application or parenteral administration of broad-spectrum antibiotics and vitamins. The zoonotic importance of the disease should be further studied as humans today are highly susceptible to smallpox a very related and devastating virus eradicated from the globe. This review address an overview on the epidemiology, zoonotic impacts, diagnostic approaches and the preventive measures on camelpox.
Nko Samuel Bassey, Clement Whong, Saleh Alhaji Ado
Scientific Journal of Microbiology, Volume 6, pp 166-173; https://doi.org/10.14196/sjm.v6i7.2552

Abstract:
In this study, seven yeast isolates were obtained altogether from both spoilt fruit and brukutu alcoholic drink using the spread plate method. Yeast strains were isolated from burukutu alcoholic drink, spoilt pineapple fruit and spoilt watermelon fruit, confirmed using API Test kit and subjected to flocculation, ethanol tolerance, hydrogen sulphide, temperature tolerance test and stress tolerance test to determine their fermentative potentials. In all 7 species Candida sphaerica, Pichiaspp, Candida guilliermondii, Candidafamata Candida pelliculosa, Cryptococcus humicola and Kloeckeraapiculata were isolated. Candida sphaerica, Candida famata and Kloeckeraapiculata displayed the highest flocculation ability while only Candida famata and Candida pelliculosa survived 15% ethanol concentration. Hydrogen sulphide test showed that only Candidafamata and Candida pelliculosa can survive the presence of hydrogen sulphide. All yeast isolated grew very well at 25°C except Pichia species while only Candida pelliculosa grew at 45°C. All the seven yeast isolated survived the first and second stage of stress while only Candida pelliculosa survived the last stage.
Amanuel Bekuma
Scientific Journal of Microbiology, Volume 6, pp 156-161; https://doi.org/10.14196/sjm.v6i5.2557

Abstract:
Biopreservation refers to the use of antagonistic microorganisms or their metabolic products to inhibit or destroy undesired microorganisms in foods to enhance food safety and extend shelf life. In order to achieve improved food safety and to harmonize consumer demands with the necessary safety standards, traditional means of controlling microbial spoilage and safety hazards in foods are being replaced by combinations of innovative technologies that include biological antimicrobial systems such as lactic acid bacteria (LAB) and/or their metabolites. The antagonistic properties of LAB derive from competition for nutrients and the production of one or more antimicrobial active metabolites such as organic acids (lactic and acetic), hydrogen peroxide, and antimicrobial peptides (bacteriocins). Nowadays the use of LAB bacteriocins is considered an integral part of hurdle technology. Their combined use allows most pathogenic and spoilage bacteria to be controlled and also extend their inhibitory activity spectrum to such intrinsically resistant organisms as the Gram-negative bacteria.
Shanthi Subbarayan, Bharathi Vadivel, ,
Published: 24 March 2017
Scientific Journal of Microbiology, Volume 6, pp 149-155; https://doi.org/10.14196/sjm.v6i3.2340

Abstract:
Andrographis paniculata (Acanthaceae), a potential medicinal plant, distributed in waste lands, open forests in India. A. paniculata is used in the treatment of typhoid, snake bite, scorpion sting, malaria and cancer. The acetone, chloroform and aqueous extracts were evaluated for its anthelmintic activity against adult earth worms (Phertima prosthuma). Three concentrations (10, 50 and 100 mg/ml) of each extract were which involved the determination of time of paralysis and time of death of the test worms. It was found that the acetone and aqueous fraction of Andrographis paniculata of was proved to be one of the best alternative cum effective herbal remedy to control helminthic infections.
Aliyu Sarki Baki, Abdulrahman Bello, Abubakar Aliyu Salihu
Published: 26 January 2017
Scientific Journal of Microbiology, Volume 6, pp 142-148; https://doi.org/10.14196/sjm.v6i1.2467

Abstract:
Onychomycosis is a fungal infection of the nail, which occurs worldwide with Dermatophytes as the most common causal agents although yeast and moulds are also involved. The diagnosis usually involves direct microscopy and culture to prove actual existence of onychomycosis. A total of 30 samples were tested using 20% KOH and culture plates of Sabourad Dextrose Agar (SDA) which was mixed with streptomycin as an antibiotic. A total of 80.0% samples were identified as positive by culture, among which, 46.7% were KOH positive and 33.3% were KOH negative. 20.0% were culture negative. The predominant pathogen was yeast 37.43%, followed by dermatophyte 33.33% and the moulds with 24.12% of the cases recorded. Onychomycosis was observed to be common between the age of 41-50 with the occurrence of 50%, and 50% in both male and female patients attending the hospital. The research highlighted that the yeast was a predominant pathogen in Ammannawa General Hospital Sokoto, patients should be well informed on the need to take their health and personal hygiene seriously, and also adhere to treatments. Based on the result obtained, it was recommended that people should avoid going barefoot in public places, and keep feet cool and dry. To educate patients on the need to improve their health and personal hygiene. Patients should endeavor to apply antifungal powder/spray to the inside of their shoes once a week or more, and they should also comply with all treatment protocol.
Aliyu Sarki Baki, Abdulrahman Bello, Ummul Kalthum Mohammad, Ahmad Aliyu Farouq, Muhammad Aliyu Yahaya, Auwal Gambo, Umar Salisu Ahmad, Shehu Zaid
Published: 22 November 2016
Scientific Journal of Microbiology, Volume 5, pp 134-141; https://doi.org/10.14196/sjm.v5i11.2460

Abstract:
Yeast are chemoorganotrophs that obtain carbon mostly from hexose sugars, such as glucose and fructose or disaccharides such as sucrose and maltose and these carbon sources are frequently present in yoghurt, cow milk and other fermentable products. Three fungal isolates were isolated from traditionally fermented cow milk which are saccharomyces cerevisiae, Saccaharomyces pombae and Pichia anomala. Isolation was carried out by serially diluting the samples in the laboratory, which were fermented at room temperature for 24 hours. All of the yeast isolated are glucose and sucrose fermentors. Biosurfactant was also produced after growing the yeast in a prepared Sabouraud Dextrose Agar (SDA) containing 1.0 ml of crude oil at the temperature range between 27-30 0C for 72 hours. After incubation oil displacement test was carried out to determine if biosurfactant can be produced from the yeast, from the result Saccharomyces cerevisiae and saccohromyes pombae showed clear zones with diameter 96.0 mm/cm3 and 40.4 mm/cm3 respectively. Conclusively, biosurfactant was extracted from Saccharomyces cerevisiae which was measured with amount of 220 mg/ml while Saccharomyces pombae do not produced biosurfactant when grown in Sabouraud Dextrose agar (SDA) containing 1.0 ml of crude oil at a room temperature for 24 hours.
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