Acta Scientiarum. Biological Sciences

Journal Information
ISSN / EISSN : 1679-9283 / 1807-863X
Published by: Universidade Estadual de Maringa (10.4025)
Total articles ≅ 1,144
Current Coverage
SCOPUS
DOAJ
Archived in
SHERPA/ROMEO
Filter:

Latest articles in this journal

Camila De Lima Faustino, Rosa Maria Dias, Silvia Regina Ferreira, Henrique Ortêncio Filho
Acta Scientiarum. Biological Sciences, Volume 43; https://doi.org/10.4025/actascibiolsci.v43i1.52030

Abstract:
We evaluated the structure of a community of frugivorous bats using composition and abundance patterns, niche amplitude and food overlap of these animals in four Atlantic Forest fragments, each one exposed to different conservation realities. For twelve months, we captured six bat species and found the seeds of 13 species of pioneering plants in 158 fecal samples. The most abundant bat species were Artibeus planirostris (25.4%), Artibeus lituratus (24.1%) and Carollia perspicillata (23.9%). Only one fragment (Fazenda Unida), the most conserved area, exhibited a significantly different composition and abundance of species. We found low trophic niche amplitude values (<0.60), associated to high food overlaps. Our results suggest that bats can adjust their foraging strategy to deal with food availability variations. By favoring pioneering plant species, the fragmentation process noted of the studied areas creates an attractive environment for bats more tolerant to this type of disturbance. The sampled areas represent important secondary forest remnants in southern Brazil that require attention to avoid an even greater loss of bat diversity.
Patrícia Alexandre Evangelista, Mayara Andrade Martins de Souza, , Natan De Jesus Pimentel-Filho
Acta Scientiarum. Biological Sciences, Volume 43; https://doi.org/10.4025/actascibiolsci.v43i1.52901

Abstract:
This study aimed to evaluate the microbiological quality of the water of four ponds used for irrigation on the Lagoa do Sino Farm, as well as to perform the genotypic characterization of virulence factors in Escherichia coli isolates. Sampling was conducted for 11 months, between 2015 and 2016. Samples were analyzed for the presence of thermotolerant coliforms, E. coli and heterotrophs. DNA was extracted from E. coli isolates, followed by genotypic characterization by polymerase chain reaction. Agricultural activities and pesticides used in the sampling period were documented in order to assess possible relationships between agricultural activities and microbiological water quality. The absence of suitable riparian vegetation around all the ponds was observed, benefiting the entry of organic matter and contaminants in the water body. A high index of thermotolerant coliforms in some months indicated the possibility of the transmission of pathogenic microorganisms in these ponds. The values found in some months were above the regulatory limits for water potability and water intended for irrigation. The agrochemicals used in the period seem to influence the results obtained. All 17 E. coli isolates showed at least one of the virulence genes estA, stx1, stx2, and aatA, indicating enterotoxigenic, enterohaemorrhagic or enteroaggregative nature. The presence of E. coli in the waters may be associated with the presence of animals. The water samples analyzed are not suitable for irrigation of vegetables that are consumed raw and/or low lying fruits ingested without skin removal. It is essential to broaden the control of the use of chemicals, as well as the preservation of riparian vegetation to improve the quality of water used in the farm's agricultural activities.
Karina Guollo, Américo Wagner Junior, Carlos Kosera Neto, Juliana Cristina Radaelli
Acta Scientiarum. Biological Sciences, Volume 43; https://doi.org/10.4025/actascibiolsci.v43i1.55816

Abstract:
Studies on the Myrtaceae family are mainly distributed in floristic surveys, reproductive studies of plant communities or related to family taxonomy. Based on this, the objective of the present study was to elucidate aspects of floral and reproductive biology and, of floral visitors from Campomanesia guazumifolia (Cambess.) O. Berg., a neglected species. Information was obtained on floral morphology and morphometry, anthesis, nectary, and structures attractive to pollinators, characterization of pollinators, receptiveness of stigma and maturing of the androecium components, and characterization of the reproductive system. Sete-capote tree has hermaphrodite flowers, and the floral opening occurs mainly during the daytime. Pollen grains was the main resource offered to pollinators. The flowers had mellow sweet odor, attracting mainly native bees and Apis melifera, which was characterized as effective pollinators. The species presents high reproductive efficiency and could be considered self-compatible; however, fertilization also occurs by cross-pollination.
Andréia Anschau, Katira Da Mota Huerta, Tatiane Vieira Rêgo, Janaina Mardioni Gonçalves de Oliveira, Carina Molins Borba, , , Janaína Fernandes De Medeiros Burkert
Acta Scientiarum. Biological Sciences, Volume 43; https://doi.org/10.4025/actascibiolsci.v43i1.54966

Abstract:
Many food, cosmetic and pharmaceutical industries have increased their interest in short-chain esters due to their flavor properties. From the industrial standpoint, enzyme reactions are the most economical strategy to reach green products with neither toxicity nor damage to human health. Isoamyl butyrate (pear flavor) was synthesized by isoamyl alcohol (a byproduct of alcohol production) and butyric acid with the use of the immobilized lipase Lipozyme TL IM and hexane as solvents. Reaction variables (temperature, butyric acid concentration, isoamyl alcohol:butyric acid molar ratio and enzyme concentration) were investigated in ester conversion (%), concentration (mol L-1) and productivity (mmol ester g-1 mixture . h), by applying a sequential strategy of the Fractional Factorial Design (FFD) and the Central Composite Rotatable Design (CCRD). High isoamyl butyrate conversion of 95.8% was achieved at 24 hours. At 3 hours, the highest isoamyl butyrate concentration (1.64 mol L-1) and productivity (0.19 mmol ester g-1 mixture . h) were obtained under different reaction conditions. Due to high specificity and selectivity of lipases, process parameters of this study and their interaction with the Lipozyme TL IM are fundamental to understand and optimize the system so as to achieve maximum yield to scale up. Results show that fusel oil may be recycled by the green chemistry process proposed by this study.
Marcela Padilha Iastremski, Juliano Tadeu Vilela de Resende, Cristhiano Kopanski Camargo, Marcos Guilherme Ruthes, Marco Antonio Pereira Santos
Acta Scientiarum. Biological Sciences, Volume 43; https://doi.org/10.4025/actascibiolsci.v43i1.53105

Abstract:
The use of genotypes more adapted to climatic conditions can contribute to increase the yield of onion producers. The goal of this study was to evaluate the agronomic performance of 15 onion genotypes in the soil and climatic conditions of Guarapuava, state of Paraná. The study was conducted in the experimental area of Horticulture, Cedeteg campus, Universidade Estadual do Centro-Oeste (UNICENTRO), Guarapuava, state of Paraná, Brazil, from July to November 2018. The experimental design used was randomized blocks, with four replications, and the treatments consisted of four commercial cultivars Optima F1, Bella Dura, Sirius F1, Soberana F1 and eleven experimental genotypes N1, N2, N3, N4, N5, N6, N7, N8, N9, AF4241 and AF4243. Biometric characteristics of the plants, production components and early flowering were evaluated. Plants presented between 6 and 9 leaves, in which N1, N3, N4 and N6 had less than 7 leaves, differing statistically from the others. The cultivar Optima F1 and the genotypes N2, N3, N5, N6, N7 and N8 presented the tallest plants, with 66.1 to 76.0 cm. The pseudostem diameter did not differ significantly between genotypes, showing values between 15.2 and 20.4 mm. Total productivity was higher in genotypes N2, N6, N5, N4, N3, N7 with values from 43.6 to 50.3 t ha-1. The highest average bulb mass was found in N2, N4, N6, Sirius F1, Optima F1 and Soberana F1, with 74.2 to 91.1 g bulb-1. Regarding the commercial classification, the genotypes N2, N4, N5, N6 and the cultivars Sirius F1 and Soberana F1 presented more than 50% bulbs of classes 3+3C+4. Early flowering did not occur in any of the analyzed genotypes. The use of cultivars with high productive performance and adapted to climatic conditions, when handled properly, can result in greater yield and quality of bulbs.
Amanda Brito Costa, , Ricardo Evangelista Fraga, Agda Alves da Rocha, Patrícia Belini Nishiyama, Matheus Santos dos Anjos, Joaquim Jorge Silveira Buchaim, Mariane Amorim Rocha
Acta Scientiarum. Biological Sciences, Volume 43; https://doi.org/10.4025/actascibiolsci.v43i1.53450

Abstract:
Although crustaceans are traditionally preserved in liquids (formaldehyde and/or ethyl alcohol), those substances tend to alter their morphological aspects. Glycerin, used in human anatomy, is considered a good substitute for formaldehyde, as it preserves animals in states similar to in vivo conditions. There are no records in the literature, however, concerning the use of glycerin for conserving invertebrates. The objective of this work was to elaborate and evaluate alternative techniques for conserving the crustacean Ucides cordatus (Linnaeus, 1763). Six fixatives (1, 3, 4 and 5% formaldehyde, 70% alcohol, and dietrich solution) and two controls (positive and negative) were tested, as well as the effects of freezing before fixation on the integrity of U. cordatus specimens. Our results were evaluated with respect to nine variables. The treatments that demonstrated the best aesthetic results were 4% formaldehyde and 70% ethyl alcohol. The freezing of the animals resulted in brittle organs in all treatments tested. The technique discussed here is extremely promising for the conservation of animals for educational purposes, as it produces preserved specimens that are aesthetically similar to their in vivo conditions.
Isabella Ferreira Silva, Letícia Fagundes Papa, Pedro Gontijo Carneiro, Mariane Cristina Schnitzler, Silmara Nunes Andrade, Rosy Iara Maciel Azambuja Ribeiro, Fernando Pilla Varotti, Ralph Gruppi Thomé, Hélio Batista Santos
Acta Scientiarum. Biological Sciences, Volume 43; https://doi.org/10.4025/actascibiolsci.v43i1.52612

Abstract:
Multiwalled carbon nanotube (MWCNT) has been broadly used in several sectors of society. This material when exposed to the environment might reach the aquatic animals and cause toxic effects. Here, it was evaluated the MWCNTs toxicity in melanomacrophages primary culture that was submitted to 1 µ gm L-1 MWCNTs for 24 hours. After exposition to MWCNT, 48 and 59% liver and spleen melanomacrophages were healthy, respectively. The control group presented 85% viability. Phagocytosis activity of melanomacrophages was observed by presence of black inclusions in cytoplasm. The findings indicate MWCNT was cytotoxic to melanomacrophages, where its release and effect into aquatic environment must be more studied. Finally, the melanomacrophages present large potential as experimental model for evaluation of carbon-based nanomaterial toxicity.
Karen Cristina Fialho dos Santos, Deyse Maria de Souza Silveira, Antônio Da Silva Souza, Jucieny Ferreira de Sá, ,
Acta Scientiarum. Biological Sciences, Volume 43; https://doi.org/10.4025/actascibiolsci.v43i1.52866

Abstract:
In vitro multiplication is an important tissue culture technique that is capable of efficiently producing seedlings at any scale. It is a propagation method based on the aseptic culture of small propagules in a suitable culture medium to enable plant regeneration. Multiplication experiments conducted in vitro to set protocols adapted to wild Manihot species have used modified mineral salts and MS vitamins as basic culture medium. Here, 25 treatments based on combinations of the regulators benzylaminopurine (BAP) and naphthaleneacetic acid (NAA) at 0, 0.025, 0.05, 0.075, and 0.1 mg L-1 were used for in vitro multiplication of three genotypes of wild Manihot species (M. violaceae Pohl Müll. Arg., M. pseudoglaziovii Pax & Hoff., and M. flabellifolia Pohl). Plant height and the number of 1 cm minicuttings, number of roots, shoots, green leaves and senescent leaves were recorded 120 days after explant inoculation. M. violaceae Pohl. Müll. Arg. and M. flabellifolia Pohl. presented favorable results with 0.05 and 0.025 mg L-1 NAA, respectively. Culture medium lacking NAA and BAP favored the in vitro growth of M. pseudoglaziovii Pax & Hoff.
Luiz Gustavo Antunes Pessoa, Leonardo Antunes Pessoa, Éverton Da Silva Santos, Eduardo Jorge Pilau, José Eduardo Gonçalves, Regina Aparecida Correia Gonçalves, Arildo José Braz de Oliveira
Acta Scientiarum. Biological Sciences, Volume 43; https://doi.org/10.4025/actascibiolsci.v43i1.53075

Abstract:
Plant tissue culture has emerged as an important tool to produce bioactive compounds from various plant species, including the sustainable production of limonoids that are receiving considerable attention due to the benefits associated with human health such as anticancer activities. The purpose of the present study was to evaluate the capacity of limonoids aglycone production from callus culture from sweet orange cv. Pera (Citrus sinensis) seeds and identify the compounds produced in this cell line. Callus induction occurred in Murashige and Skoog medium supplemented with 2,4-dichlorophenoxyacetic (2,4-D), malt extract, agar and coconut water. For the analysis and identification of the limonoids, CG-MS-EI ion-positive mode and UPLC-QTOF-ESI were used operating in positive and negative mode. An intense peak corresponding to limonin appeared in the callus extracts at a retention time of 58.1 min. in CG-MS-EI and four major limonoids aglycone by positive ion mode UPLC-QTOF-ESI: limonin, nomilin, deacetylnomilin, and nomilinic acid. The culture medium was efficient at the bioproduction of limonoids aglycone in callus cultures of C. sinensis seeds. Therefore, data obtained from UPLC-QTOF-ESI proved its importance at identifying new compounds that benefit human health, and may assist future work in the identification of known or new limonoids in Citrus species and related genera.
Bruna Dos Santos Menezes, Kátia Dos Santos Morais, Aparecido Almeida Conceição, Juliana Gomes Barreto Souza Leite, Fábia Giovana Do Val de Assis, Félix Gonçalves Siqueira, Patrícia Lopes Leal
Acta Scientiarum. Biological Sciences, Volume 43; https://doi.org/10.4025/actascibiolsci.v43i1.48257

Abstract:
Aspergillus niger KIJH was grown in solid and submerged fermentation using leaves and roots (with and without bark) of plants typically from Brazilian semiarid as substrate to produce a multienzymatic extract, which was characterised for its potential biotechnological applications. Solid-state fermentation (SSF) was applied to select the most promising plants biomass as induction substrates for the production of hydrolytic enzymes by fungus. The best biomasses were used as substrate in submerged fermentation (SmF) assays at two scales. Samples of up scale fermented culture were partially purified by ultrafiltration and activity and pH and temperature stability of CMCase and xylanase were evaluated. A. niger KIJH produced hydrolytic enzymes under SSF containing unconventional plants biomass from Brazilian semiarid. In SmF conditions, maximum CMCase (0.264 U mL-1) and xylanase (1.163 U mL-1) activities were induced by Jacaratia corumbensis. Scaling up the SmF to 500 mL of medium was able to maintain constant the production of CMCase (0.346 U mL-1) and xylanase (1.273 U mL-1) on the fermented culture. Ultrafiltered and concentrated extract presented CMCase activities practically constant in all temperature ranges (30-80°C) and pH (3.0-9.0), while xylanase optimum activity temperature was 50°C and pH in the range of 3.0 to 5.0. CMCase activity remained stable for 24 hours at 50°C and xylanase was reduced in 53% after two hours incubation at the same temperature. CMCase and xylanase obtained by A. niger KIJH cultivated in submerged culture containing J. corumbensis as carbon source may have application in biotechnology processes that require enzymes that remain active under routine extreme conditions.
Back to Top Top