Bioma : Berkala Ilmiah Biologi

Journal Information
ISSN / EISSN : 14108801 / 25982370
Current Publisher: Diponegoro University (10.14710)
Total articles ≅ 162
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Dzunnuroini Khanif Makhfudhoh, Jafron Wasiq Hidayat, Fuad Muhammad
Bioma : Berkala Ilmiah Biologi, Volume 20, pp 145-153; doi:10.14710/bioma.20.2.145-153

Abstract:Plankton is organisms that had an important role in water as natural feed of fishes and as an indicator. This study aimed to identify water quality which was suitable for fish cultivation reviewed from physics-chemicals parameters of water and its saprobic status in Tambak Lorok Tanjung Mas Kota Semarang. Sampling were take based on 5 stations that could represented from plankton variety of condition water stability . Plankton samples were by using net plankton number 25, then preserved with alcohol 70% and formalin 4%. Variables of ponds water quality measured were temperature, salinity, dissolved oxygen, pH, turbidity, N concentration, and Pb content. Identification of planktons used SRC method under microscop. The result of plankton analysis in Tambak Lorok water showed 42 phytoplankton species, the commonly species found are Coscinodiscus centralis, Coscinodiscus granii, Gyrosigma sp., Navicula radiosa, Pandorina sp., and Melosira sp. Meanwhile, zooplankton found was 22 species with common species are Cyclops, Harpaticoida, Tricodesmium evythraeum, Brachinus plicatilis, and Lensia subtilis. Phytoplankton diversity index are 0.057-0.367 and equity index are 0.025-0.176. Zooplankton diversity index are 0.247-0.360 and equity index are 0.157-0.201. This indices showed that there were low level of species diversity and community stability, dominance since the species occurred. Measurement of water quality variables indicated that Tambak Lorok had been contaminated and needed a preliminary management before d be used as fish cultivation ponds, because the analysis result was not appropriate with Government water quality regulation. Based on the measurement, soprobic index categories on α/β-Mesosaprobic to Oligosaprobic phase and the degree of pollution is very mild to moderate. Keywords : Community Structure, plankton, Tambak Lorok, Tanjung Mas, Semarang
Sri Isdadiyanto
Bioma : Berkala Ilmiah Biologi, Volume 20, pp 140-144; doi:10.14710/bioma.20.2.140-145

Abstract:The objective of this study was to analyze the influences of 50% kombucha tea level on wall thickness and lumen diameter of coronary artery in rats. Sixteen male Rattus norvegicus rats, 2 months of age were used as experimental animals, with treatment kombucha tea that has been fermented for 6, 9 and 12 days at the temperature of 25oC per oral. The research design used to a completely randomized with 4 treatment (for 4 weeks) and 4 replications, i.e., P0 = control, without of kombucha tea, P1 = water + 1,8 ml kombucha tea that has been fermented for 6 days in the morning and afternoon, P2 = water + 1,8 ml kombucha tea that has been fermented for 9 days in morning and afternoon, P3 = water + 1,8 ml kombucha tea that has been fermented for 12 days in morning and afternoon. Variables measured were wall thickness and lumen diameter of coronary artery. Data were analyzed with ANOVA followed by Duncan test at 95% confidence level were performed using SPSS version 10,0. The result showed that kombucha tea did not influences the wall thickness and lumen diameter of coronary artery in rats. Key words: Rattus norvegicus, 50% kombucha tea level, wall thickness, lumen diameter, coronary artery
Vicka Kusuma Sari, Udi Tarwotjo, Mochamad Hadi
Bioma : Berkala Ilmiah Biologi, Volume 20, pp 105-112; doi:10.14710/bioma.20.2.105-112

Abstract:Aedes aegypti is the main vector of dengue fever disease. The most effective way to prevent dengue fever is cutting the spreading chain of dengue fever by controlling the vector with using conventional chemical insecticides. The used of insecticide intensively has became the main factor of resistance in Tembalang, then monitoring is needed to determine the change of A. aegypti susceptibility status against the insecticide. The aim of this research is to determine the sensitivity of A. aegypti population from five locations in Tembalang against pyrethroid synthetic insecticide, and to determine the validity of the diagnostic concentration as a method of monitoring resistance A. aegypti population in Tembalang. This research was conducted in Laboratory of Ecology and Biosystematics Department of Biology Faculty of Science and Mathematics, Diponegoro University Semarang. The procedure of this research were: larvae collection (A. aegypti), insect breeding test, sensitivity test including bioassay, determination of diagnostic concentration and validation. The result showed that the susceptibility level of five population was not significantly different since all populations are still susceptible, with the LC50 range between 0,0031-0,0043% and FR range between 1-1,39 (
Nabilah Aini, Yulita Nurchayati, Swa Suedy
Bioma : Berkala Ilmiah Biologi, Volume 20, pp 123-132; doi:10.14710/bioma.20.2.123-132

Abstract:Red spinach is one of plants containing anthocyanin pigment which has various functions as food and beverage dye, and play a role in the field of health. Anthocyanin biosynthesis in plants is influenced by environmental factors, one of them are nutrients. One of the nutrients needed by plants and part of the anthocyanin biosynthesis pathway is copper (Cu). Cu can be added in the form of a fertilizer such as Na2CuEDTA. This research aims to study the effect of soaking the red spinach in Na2CuEDTA solution on growth and anthocyanin content, and knowing the optimal Na2CuEDTA concentration for growth and anthocyanin content of red spinach. This research conduct to Complete Random Design of single factor pattern with 5 treatments and 5 replications which are aquades, nutrient solution, nutrient solution+Na2CuEDTA 5 ppm, nutrient solution+Na2CuEDTA 10 ppm, nutrient solution+Na2CuEDTA 20 ppm. Sprouts red spinach that already has 4 leaves soaked in the treatment solution for 10 days, then the plants moved into a soil medium inside the pot for 20 days. The analyzing data which is used is ANOVA continuing with further testing of Duncan Multiple Range Test (DMRT) at the 95% significance level. The result of this research show that soaking of red spinach in Na2CuEDTA solution did not show any significant effect on growth. The growth of red spinach plant is more influenced by nutrient solution while the anthocyanin contents is influenced by nutrients in soil medium. Key words : Red Spinach, Copper, Anthocyanin, Na2CuEDTA
Nasrul Fathoni, M Anwar Djaelani, Sri Isdadiyanto
Bioma : Berkala Ilmiah Biologi, Volume 20, pp 78-85; doi:10.14710/bioma.20.2.78-85

Abstract:Kombucha tea beverage obtained by fermenting sweetened green tea for 12 days with Acetobacter xylinum and Saccharomyces which produce various kinds of organic acids, vitamins, and acts as a probiotic. The role of kombucha tea as a growth promoter is to that improve the metabolic process in the digestion of broiler chickens, so that nutrients can and fulfilled optimally for growth and development. The aim of this research was to analyze the sceletal muscle glycogen glycogen (Gallus gallus) after treat with kombucha tea in drinking water. Kombucha tea used is the result of fermentation of green tea for 12 days. This research used DOC chickens (Day Old Chicken) as much as 20 randomly divided into 4 treatments with concentration ie 0%, 10%, 20%, and 40% kombucha tea in drinking water for 32 days. The variables measured were skeletal muscle glycogen levels, body weight, feed consumption and drink consumption. The data obtained were analyzed using ANOVA followed by Duncan Test with 95% confidence level using SPSS 17.0 software. The results of this study showed that kombucha tea on skeletal muscle glycogen and feed consumption showed no significant difference, while on body weight and drink consumption showed significantly different results. Based on the results of the study, it can be concluded that giving kombucha tea in drinking water to a concentration of 40% has not been able to affect the skeletal muscle glycogen in broiler chickens. Key words: Kombucha tea, broiler chicken, glycogen skeletal muscle
Riani Nurasri, Enny Yusuf Wachidah Yuniwarti, M Anwar Djaelani
Bioma : Berkala Ilmiah Biologi, Volume 20, pp 133-139; doi:10.14710/bioma.20.2.133-139

Abstract:Virgin Coconut Oil (VCO) and olive oil have been widely applied in the field of health. Saturated fatty acid content of VCO dominates the total fatty acid content of about 85%. Olive oil contains a lot of oleic acid, which is one type of unsaturated fatty acid (mono-unsaturated fatty acid). This study aimed to analyze the kidney microanatomy rat (Rattus norvegicus) after supplementation with VCO and olive oil in the feed. This study used 25 rats were divided into five treatment groups, P1 was the control group, P2 was a group with VCO 5 mL / kg feed, P3 was a group with VCO 10 mL / kg of feed, P4 was a group with olive oil 5 mL / kg feed and was a group with olive oil 10 mL / kg of feed. The results of ren weight and diameter rat glomerular analyzed using analysis of variance (ANOVA). Research design used was completely randomized design with five treatments and five replication. Results of studies on weight ren showed no significant difference between treatments P2, P3, P4 and P5 to the control (P1). The results of the glomerular diameter shows there is significantly difference between the diameter of the glomerulous treatment P2 and P3 with control (P1), while in treatment P4 and P5 are not significantly different from the control so that it can be concluded that the administration of the VCO and olive oil at various concentrations do not give rise to different responses to weight ren. Olive oil does not increases glomerular diameter white mice, while VCO at a dose of 5 mL / kg feed and 10 mL / kg of feed can increased glomerular diameter mice. Keywords: ren weight, glomerular diameter, Virgin Coconut Oil (VCO), olive oil.
Andy Wicaksono, Fuad Muhammad, Jafron Wasiq Hidayat, Damang Suryanto
Bioma : Berkala Ilmiah Biologi, Volume 20, pp 113-122; doi:10.14710/bioma.20.2.113-122

Abstract:Milkfish is one of the main commodities in the field of fishery and is known to be the most cultivated. One of the important’s factors to the success of milkfish cultivation is feed availability, both quality and quantity. Feed quality is an important aspect that must be fulfilled because of its relation to nutrients required for the fish growth. Azolla pinnata has a good potential that can be used as an additional ingredient for the fish growth because of its protein-rich content. This study is aimed to examine the effect of Azolla pinnata powder feed on growth of milkfish (length and body weight). This research was conducted in May – July 2017 at Aquatic Animal Health Management Laboratory, Center of Brackish Water Aquaculture of Jepara. The research design used is a Complete Randomized Design (CRD), using five treatments with different concentrations of Azolla pinnata powder, i.e. 0% as control, 10%, 20%, 30%, and 40%. Each treatments consist of three repeatitions. The parameters observed were milkfish growth (length and body weight) and dissolved oxygen (DO). Data were analyzed by Analysis of Variance (ANOVA) method. If the data showing significant differences it will be continued with Least Significance Different (LSD) test. The results showed that the administration of Azolla pinnata powder on the feed significantly influenced the growth (lenght and body weight) of milkfish. The most optimal and efficient feed composition for milkfish growth is done by feeding 40% of Azolla pinnata powder. The overall dissolved oxygen (DO) is still in suitable condition for the cultivation of milkfish. Key Words : Azolla pinnata, dissolved oxygen, feed nutrition, milkfish
Ryan Hilda Wandita, Sri Pujiyanto, Agung Suprihadi, Ratih Dewi Hastuti
Bioma : Berkala Ilmiah Biologi, Volume 20, pp 9-16; doi:10.14710/bioma.20.1.9-16

Abstract:Onions (Allium cepa L.) is one of the leading horticultural commodities in Indonesia and is often used as seasoning and traditional medicine. Onion has a high economic value and fluctuating prices so that domestic onion production needs to be improved, one of them with a presence of endophytic bacteria that act as plant growth promoting agent or Plant Growth Promoting Bacteria (PGPB). Endophytic bacteria isolated from the root, leaves, and bulbs. In this research has been tested endophytic bacteria of onion plants from Garut regency which has PGPB factors such as able to dissolve phosphate, and produce HCN. The results obtained 251 isolates of endophytic bacteria. Based on the characterization results, the superior isolates capable of dissolving phosphate with an average diameter of 0.45 cm is isolate II.B.1D.3, and 11 isolates capable of producing high HCN. These isolates can be used as PGPB agents so that they can be useful in increasing plant growth and onion production and biocontrol in suppressing pathogens. Keywords: PGPB, endophyte, onion, phosphate, HCN
Lania Yuchanitz Fatma, J Jumari, Sri Utami
Bioma : Berkala Ilmiah Biologi, Volume 20, pp 17-24; doi:10.14710/bioma.20.1.17-24

Abstract:Dioscorea spp (Yam) is one of the potential local food producers. It has advantage of high species diversity, containing high carbonhydrate and easy to grow. The aim of this research is to determine the variants of Dioscorea spp, examine the structure and composition of trees around Dioscorea spp habitat and determine the enviromental factors of Dioscorea spp habitat. The research was carried out in May-June 2017. The sampling technique was using exploration and quadratic method by plotting the trees into 5 squares (10 m x 10 m) with diameter of stem more than 10 cm. The name of each species, number of individuals and stem diameter measurement of each plot were recorded. The data of diversity Dioscorea spp and environmental factors were analyzed by sort of descruptive set and the trees was analyzed by vegetation analysis. The results showed that 4 species of Dioscorea spp. There are D. hispida, D. bulbifera, D. alata and D. esculenta. D. alata has 3 variants called uwi ireng, uwi ungu and uwi bangkulit. D. esculenta has 2 variants called gembili bebek and gembili terpong. The trees around Dioscorea spp obtained 21 species with the most important index is banana (Musa paradisiaca) and sengon (Albizia chinensis). The environmental factors were indicated the appropiate growth of Dioscorea spp. Key words: Dioscorea spp, local food stuffs and variant types
Ella Dewani Larasati, Mg Isworo Rukmi, Endang Kusdiyantini, R Cinta Badia Ginting
Bioma : Berkala Ilmiah Biologi, Volume 20, pp 1-8; doi:10.14710/bioma.20.1.1-8

Abstract:Phosphate (P) are macronutrients that necessary for growth and development of plants, But the availability of phosphate dissolved in the ground are very limited because trend is bound to minerals ground form phosphate complexs. One of the alternative to improve the efficiency of phosphate is by utilize phosphate solubilizing bacteria. Phosphate solubilizing bacteria is bacteria capable of dissolving phosphate that is not available become available, so that it can be absorbed by plants. Peat soils can be used as a source of phosphate solubilizing bacteria because , peat soils formed from litter and organic matter so, many microorganisms which live in peat soils one of them is a phosphate solubilizing bacteria. The aim of this research is to isolat, identify bacteria to species level using Biolog system GenIII Mircoplate, and test the ability of phosphate dissolution. The isolation is done with the methods spread plate in a media pikovkaya solid and testing the ability to isolat qualitatively glimpsed clear zone around colonies, measuring phosphate solubilizing index. testing the ability isolats quantitatively by measuring solubility phosphate using spektrofotometer, and identification isolats using Biolog System. Eighteen isolates phosphate solubilizing bacteria successfully obtained from of peat soils, two isolates of them potential in solubilizing phosphate, According to Biolog system the second isolates identified as Pseudomonas tolaasii (isolate PG2T.5) and a Bacillus pumilus (isolate PG3TT.2), each is about phosphate dissolved successive of 24,81 mg/L and 22,62 mg/L Key words: Peat Soil, Isolation, Phosphate Solubilizing Bacteria, Biolog System Identification