Bioscience

Journal Information
ISSN / EISSN : 2614-669X / 2579-308X
Total articles ≅ 87
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I. Irdawati, Putri Alamanda Sari
Bioscience, Volume 4, pp 133-139; doi:10.24036/0202042106062-0-00

Abstract:
Kombucha is a fermented tea made from steeping tea and sugar which utilizes byproducts from bacterial and yeast symbiosis in the form of acidic compounds and nata (cellulose). Kombucha fermentation process produces a variety of substances that are efficacious to improve body health and ward off poisons. One method of kombucha fermentation can be done by cell immobilization method or enzymes because it can be repeated fermentation. The purpose of this study was to determine the quality of kombucha tea by immobilized cells in several different teas.This research is an experimental research. The research was conducted from May - August 2019 in the Microbiology Laboratory of the Faculty of Mathematics and Natural Sciences, Padang State University. The quality test of kombucha tea by immobilized cells can be determined by measuring the thickness of nata, pH, and total acetic acid of Kombucha tea. Data were analyzed using ANOVA followed by further tests DMRT with a level of 5%. The results showed that the best quality of kombucha tea by immobilized cells was green tea with a thickness of 0.53 cm nata, pH 5.62 and total acetic acid (%) 4.40.
Rita Maliza, Jannati Aulah, Oktira Roka Aji
Bioscience, Volume 4, pp 162-171; doi:10.24036/0202042108692-0-00

Abstract:
Coffee fruit skin is a waste from coffee bean processing. Arabica coffee bean extract (Coffea arabica L.) is known to contain anthocyanin compounds, polyphenols, beta-carotene, and vitamin C which have the potential as antimicrobial agents. The purpose of this study is identification and anaysis the optimal concentration of Arabica coffee peel extract which inhibits the bacteria Escherichia coli and Staphylococcus aureus. This study used an experimental method consisting of 3 extract concentration treatments, positive control, and negative control with 3 repetitions. The treatments tested were 25%, 50%, 75% coffee fruit peel, negative control (sterile distilled water) and positive control (Ampicillin 2 µg / ml). The bacterial test was carried out using the in vitro method. The parameters measured were the amount of inhibition zone (mm) formed on the Mannitol Salt Agar (MSA) and Mac Conkey media. Analysis of research data using the One-way ANOVA test and Duncan test at α = 0.05. The results showed that the optimum concentration of the extract against Staphylococcus aureus was 50%, while the optimum concentration of Escherichia coli was 25%. The results of this study indicate that the extract can be an antibacterial that can inhibit the growth of Escherichia coli and Staphylococcus aureus bacteria optimally.
Febrinal Febrinal, Fingki Alvia Chandra, Rijal Satria, Ramadhan Sumarmin
Bioscience, Volume 4, pp 172-178; doi:10.24036/0202042106098-0-00

Abstract:
The purpose of this research is to describe the characteristic of the tree as birds nesting at State University of Padang , West Air Tawar, West Sumatra. This research conducted on March 2019. Data collection was using survey method. The results showed that found 14 species of tree is using by birds to nesting. The characteristic of tree is preferable by birds to using for nesting are tree and pole growth rate, monopodium and simpodium branching type, with round, oval and fountain canopy type, model leeuwenberg, attims and corner architecture model.
Syifa Fajrisani, Violita Violita, Irma Leilani Eka Putri, Des Des M
Bioscience, Volume 4, pp 179-188; doi:10.24036/0202042101106-0-00

Abstract:
The purpose of this research to determined the effect of Sargassum sp. liquid organic fertilizer in the growth of spinach plant (Amaranthus hybridus L.) using hydroponic. This research used Completely Randomized Design with six treatments and four repetitions ie: A= comparison (AB mix 10 mL/L of water), B= liquid organic fertilizer 6 mL/L of water, C= liquid organic fertilizer 8 mL/L of water, D= liquid organic fertilizer 10 mL/L of water, E= liquid organic fertilizer 12 mL/L of water, and F= liquid organic fertilizer 14 mL/L of water. Parameter observed was height of plants, number of leaves, leaf surface area, fresh weight of plants, and dry weight of plants. Data was analyzed by using the Analysis of Variants (ANOVA) and Duncan. The result showed that application of Sargassum sp. liquid organic fertilizer has been an effect on height of plants, number of leaves, leaf area, fresh weight of plants, and dry weight of spinach plant (Amaranthus hybridus L.). However, the effect was not as expected to increase the growth of spinach plant.
Sukmawati Sukmawati
Bioscience, Volume 4, pp 125-132; doi:10.24036/0202042108256-0-00

Abstract:
One of the Indonesian snacks that can be found in all regions is fish siomay. fish siomay in the city of Sorong, there have been no reports on both the level of community preference and food security. The purpose of this study was to determine the level of community preference and the total number of microbial plate numbers snacks on fish siomay in the city of Sorong, West Papua. The method used in this research is descriptive method and experimental method. The results showed that for the hedonic test had a varying degree of preference for each sample, while the total number of plates did not exceed the maximum limit, then for the correlation of the two tests showed there was a relationship. The hedonic test results from the five samples show the best level of preference is the BS sample, and the EH sample, then the AQ sample, and the DS sample and the lowest preference level is the CR sample. While the sample of fish siomay scattered in the city of Sorong was suitable for consumption.
Junardi Junardi, Ina Idola, Tri Rima Setyawati
Bioscience, Volume 4, pp 189-196; doi:10.24036/0202042109331-0-00

Abstract:
Freshwater crab Parathelphusa maindroni can live in various types of aquatic habitats. High adaptability can cause differences in body size in response to diverse habitat conditions. This species has been found at Stasiun Cabang Panti Gunung Palung National Park, West Kalimantan, in two different habitat types ununfortunately data on body size is still not available. The purpose of this study was to distinguish the morphometric characteristics of P. maindroni from two different habitats. The P. maindroni crabs were collected with traps and searched using a dip net. Morphometric analysis using 12 characters of body parts. The number of specimens was collected as many as 52 individuals, 28 individuals from river and 24 individuals from freshwater swamp habitat. The freshwater crab P. maindroni that lives in two different types of habitat can only be identified based on the width of the carapace, this character can also be used to differentiate of sex.
Witri Nofita Safitri, Irdawati Irdawati
Bioscience, Volume 4, pp 197-206; doi:10.24036/0202042105679-0-00

Abstract:
Tea can be used as a fermented drink called kombucha tea. Based on the oxidation process tea is classified into white tea, green tea, oolong tea and black tea. The compounds produced from the fermentation of kombucha tea have a fairly good antibacterial effect. This study aims to determine the antibacterial activity of kombucha tea fermentation from several variations of tea types against Escherichia coli and Staphylococcus aureus. This research is an experimental study with a completely randomized design (CRD) with 5 treatments and 3 replications conducted in May-June 2019 at the Microbiology Laboratory and the Integrated Research Laboratory of the Department of Biology FMIPA UNP. Kombucha tea used in this study was white tea, green tea, oolong tea and black tea. Antibacterial activity test was carried out by paper disc diffusion method against microbial test of S. aureus and E. coli bacteria. Research results showed that the highest antibacterial activity of kombucha tea fermentation in E.coli was found in black tea with a value of 1.64 cm and the lowest in tea white with a value of 1.04 cm. In S. aureus the highest value was obtained in black tea with a value of 2.48 cm and the lowest in white tea with a value of 1.39 cm.
Wolly Candramila, Muhammad Taufik Riandi, Dani Abdillah, Eko Widodo
Bioscience, Volume 4, pp 140-150; doi:10.24036/0202042109311-0-00

Abstract:
Carp (Cyprinus carpio L.) is one of the widely cultivated fishes in Indonesia. Utilization of organic waste from the traditional market such as chicken intestines, corn husk, and water spinach stems into artificial feed for carp (Cyprinus carpio L.) var. Majalaya is discussed in this study. Compared to the commercial feed commonly used by fish farmers in Pontianak, artificial feed with a protein content of 30% showed the best growth rate of body length and weight, and highest feed efficiency. With the proper cultivating condition, artificial feed presents a huge opportunity to be an alternative solution to reduce the cost of carp farm production.
Elsa Badriyya, Afifatul Achyar
Bioscience, Volume 4, pp 151-161; doi:10.24036/0202042108497-0-00

Abstract:
Type-2 Diabetes Mellitus (T2DM) is a metabolic disease characterized by an increase in blood glucose levels. The macrovascular and microvascular complications of T2DM can increase the risk of death in patient. SNP rs11196205 Transcription Factor 7 Like 2 (TCF7L2) gene has been associated with T2DM in several regions like Iceland, Denmark, the United State, and Thailand. SNP rs11196205 is characterized by polymorphism at position 113.047.288 from nucleotide Guanine to Cytosine. Amplification Refractory Mutation System Polymerase Chain Reaction (ARMS PCR) is a method that can be used to detect mutation or polymorphism in a DNA. The aim of this research was to design a specific primer to detect SNP rs11196205 TCF7L2 gene using ARMS PCR. The design of study was a descriptive study to determine the specific primer. The method of the research includes DNA isolation, primer design using Geneious software, amplification of targeted area using ARMS PCR, and DNA sequencing method for bioinformatics study. Based on the research, four primers to detect SNP rs11196205 TCF7L2 gene have been successfully constructed. The primers were rs11196205-F, rs11196205-R, rs11196205-F(C) as a specific primer for C allele, and rs11196205- R(G) to detect G allele, the reaction produced 3 fragments of 856bp, 559bp, and 339bp.
Rudi Yuliandri, Ramadhan Sumarmin
Bioscience, Volume 4; doi:10.24036/0202041105493-0-00

Abstract:
One of the economic benefits of birds is the development of birdwatching ecotourism. Birdwatching ecotourism can make recreation more effective and beneficial. Mount Tandikek with a high level of biodiversity is one area that has the potential to become a birdwathing ecotourism location. Based on this background, this research is important to do. this was done in June 2019 until July 2019 using the Point Count method combined with the Visual Encounter Survey (VES) method. Observation starts in the morning until late afternoon by following the hiking trail via Singgalang Gantiang from the bottom up. The data obtained were processed descriptively, and the diversity index and evenness index were calculated. The results obtained as many as 136 individuals from 35 species of birds during observation. There are 5 species of birds protected by PP. No. 7 of 1999, among them: Spilornis cheela, Spizaetus alboniger, Falco peregrinus, Todiramphus chloris and Nectarinia jugularis . The bird diversity index on Mount Tandikek is high with H '= 3.084. The average category of the binary evenness index is 0.628. The species of birds that dominated the observation were Phylloscopus trivirgatus for 16 individuals, Ficedula westerma nni for 15 individuals and Pericrocotus miniatus for 14 individuals. Bird species found on Mount Tandikek have the potential to become birdwatching ecotourism objects.
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