JURNAL PEMULIAAN TANAMAN HUTAN

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ISSN / EISSN : 1693-7147 / 2527-8665
Total articles ≅ 261
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Melati C.A, Etty Handayani, Toni Herawan, Balai Besar Litbang Bioteknologi Dan Pemuliaan Tanaman Hutan
Jurnal Pemuliaan Tanaman Hutan, Volume 15, pp 145-151; https://doi.org/10.20886/jpth.2021.15.2.145-151

Abstract:
Aquilaria malaccensis Lamk. is a type of plant that is threatened with extinction due to the high level of illegal collection from the forest. Tissue culture techniques provide an alternative to vegetative propagation on a large scale for conservation efforts. The addition of glutamine to the culture media was intended to increase the growth of A. malaccensis Lamk shoots. The research was conducted with quantitative research methods using single factor laboratory experiments with four (4) treatments arranged in a completely randomized design (CRD). The treatments were differentiated based on the addition of glutamine, namely, G0: 0 mg / l; G1: 10 mg / l; G2: 20 mg / l; G3: 30 mg / l. Data analysis used Analysis of Variance (Annova) with a level of α = 5%. The results showed that Glutamine could't affect the growth of A. malaccensis Lamk, but there was the best trend in the addition of Glutamine 20 mg/l.
Abdurrani Muin, Oktiariana Rini Lestari, Riene Suci Wulandari
Jurnal Pemuliaan Tanaman Hutan, Volume 15, pp 137-144; https://doi.org/10.20886/jpth.2021.15.2.137-144

Abstract:
The progeny trial of Shorea leprosula was established in Central Kalimantan under the IUPHHK-HA management of PT. Erna Djuliawati by planting 30 families to examine the genetic superiority of  trees as plywood raw material. The trial was laid-out using  8 blocks, 4 tree-plots and a spacing of 6 x 3 m. The aim of the study was to practice the plus trees selection in the progeny trial. The study was conducted using a survey method on phenotypically superior trees based on a diameter at breast height (dbh) baseline of ≥ 30 cm. The data collected and analyzed for the plus trees and comparison trees were total tree height (m), dbh (cm), and dbh increment (cm/yr). The results of the initial selection showed that 110 trees from 29 families were selected as candidate plus trees in the progeny trial. Futhermore, the honest significant difference test (BNJ) and scoring showed that there were 15 trees from 9 families that can be designated as plus trees.
Ilg Nurtjahjaningsih, Balai Besar Litbang Bioteknologi Dan Pemuliaan Tanaman Hutan, Lukman Hakim, Yemima Viktory Utomo, Anto Rimbawanto, Aypbc Widyatmoko, Istiana Prihatini, Maryatul Qiptiyah, Purnamila Sulistyawati, Wahyunisari Wahyunisari
Jurnal Pemuliaan Tanaman Hutan, Volume 15, pp 85-93; https://doi.org/10.20886/jpth.2021.15.2.85-93

Abstract:
Timoho (Kleinhovia hospita Linn.) has high economic value for medicine and construction. However, information on genetic diversity and conservation genetic have been limited by the lack of genetic data. Acacia mangium (mangium)This study aimed to assess genetic diversity of timoho using RAPD markers. Leaf samples of seedlings were collected from a nursery at CFBTI in Yogyakarta; the seedlings originated from plantation in Gunung Kidul (GK) and arboretum in Faculty of Forestry, GMU (FK). Seven out of 22 screened RAPD markers were stable in amplification and consisted of 61 polymorphic loci; then these markers were used to analyse genetic diversity and population structure of 48 seedlings from GK and FK. The genetic diversity and structure parameters were calculated by GenAlex software. A moderate level of genetic diversity (mean uHE=0,326) and great genetic distance between the two populations (DA=0.119) were found in this species. PCoA analysis showed that the 48 individuals were divided into two clusters according to their originated population. Appropriate conservation strategies were proposed to protect genetic diversity of this species.
Fatma Zohra, Universitas Gadjah Mada, Sri Sunarti, Balai Besar Penelitian Dan Pengembangan Bioteknologi Dan Pemuliaan Tanaman Hutan
Jurnal Pemuliaan Tanaman Hutan, Volume 15, pp 55-64; https://doi.org/10.20886/jpth.2021.15.2.55-64

Abstract:
Development of Acacia hybrid is important to be done due to their superiority such as high productivity, good wood quality, more tolerance to pest/disease and adaptive to marginal sites, although its wood durability to wood-destroyer organism attacks is still unknown yet. This study aimed to observe the wood natural durability of Acacia hybrid to drywood termites (Cryptotermes cynocephalus Light.) attack. The wood samples used were taken from 6 years old trees of Acacia hybrid in Wonogiri, Central Java. The experimental design used is completely randomized design using two factors, axial and radial of wood section with the number of trees as replication. Parameters measured were extractive content, termite mortality and wood mass loss. The results showed that the axial section of wood significantly affected the wood extractive content at the level of 0.01, while termite mortality and the mass loss of wood were not significantly affected by the wood section. The largest average percentages of termite mortality and mass loss of wood found on the middle of wood section (96.67%) and near the bark (1.14%) respectively. Based on this result, the Acacia hybrid wood is categorized as very durable wood and potential to be developed as raw material for solid wood-based industries.
Yosie Syadza Kusuma, Fifi Gus Dwiyanti, Deden Derajat Matra
Jurnal Pemuliaan Tanaman Hutan, Volume 15, pp 115-128; https://doi.org/10.20886/jpth.2021.15.2.115-128

Abstract:
Dryobalanops aromatica is a highly economic value resin-producing tree and has been known as an important international trade commodity that is widely used in the perfume, cosmetic, medicine, and wood industries. In natural habitats, this species has been found to grow well on peatland and mineral soils. The information regarding adaptive genes in Indonesian native trees to abiotic stress is still very limited. The research was conducted to analyse the differentially expressed genes (DGEs) that can elucidate the role of several upregulated and down-regulated genes under peat media and mineral soil treatments. DGE analysis was carried out using R software, Bioconductor package 'edgeR'. Using the Benjamini and Hochberg approach to control FDR (FDR 0.05), with a Log2FC 2 and p-value of 0.05, showed 320 contigs were up-regulated and 439 contigs were down-regulated, while 58129 contigs were not significantly expressed. Furthermore, this study also presents an overview of the genes involved in different pathways, such as photosynthesis, carbon and energy metabolism, hormone-related genes, nitrogen metabolism, reactive oxygen species, and transcription factor. This information will be useful in understanding D. aromatica molecular responses to stress condition that may be of use for selecting genotypes in the breeding programs or peatlands restoration
Faisal Danu Tuheteru, Universitas Halu Oleo, Sri Utami, Illa Anggraeni, Husna Husna, Agus Kurniawan, Pusat Penelitian Pengembangan Hutan, Balai Besar Litbang Bioteknologi Dan Pemuliaan Tanaman Hutan
Jurnal Pemuliaan Tanaman Hutan, Volume 15, pp 77-84; https://doi.org/10.20886/jpth.2021.15.2.77-84

Abstract:
Bitti (Vitex cofassus Reinw.) is a native plant species in Sulawesi which belongs to Verbenaceae family. This species has great potential to be developed as a house construction material, boat base material, and household utensils. Development of bitti silviculture techniques is very necessary in supporting the efforts to develop these plants. One of problems in this cultivation is disease attack. This study aimed to determine symptoms of disease attack, level of severity, determine kind of pathogen that cause disease, and analyze some factors that cause pathogen attack on bitti seedlings. The results showed that leaf spot disease was found on bitti seedlings with level of severity 33.22%. The identification results showed that the pathogen causing leaf spot disease is the fungus, namely Curvularia sp. Microclimate conditions such as temperature and humidity assumed to affect the emergence of pathogen on bitti seedlings. The attack of Curvularia sp tends to affect the growth of bitti seedlings.
Yelnititis Yelnititis, Balai Besar Litbang Bioteknologi Dan Pemuliaan Tanaman Hutan
Jurnal Pemuliaan Tanaman Hutan, Volume 15, pp 129-135; https://doi.org/10.20886/jpth.2021.15.2.129-135

Abstract:
Ramin (Gonystylus bancanus (Miq.) Kurz) is one of the most valuable timbers in Indonesia and the most over-exploited woody species, on the other hand, the success in propagation techniques are still limited. This species has been listed in Appendix II of CITES since 2004 as the number of trees and populations continuously decline. Tissue culture has been explored for mass propagation, however, this technique still faces the challenge, mainly in shoot elongation regenerated from single node explants.  The purpose  of  these experiments is  to select the best auxin (2,4-D, picloram, dan dicamba) and the best concentration of Benzyl Adenine (BA)  treatment on  somatic embryo  formation. A series of experiments of somatic embryogenesis from leaf explant for ramin were conducted at Tissue Culture Laboratory, Center for Forest Biotechnology and Tree Improvement Yogyakarta. Modified Murashige and Skoog (MS) media supplemented with 0,1 mg/L thiamine; 0,5 mg/l nicotinic acid; 0,5 mg/L pyridoxine; 2.0 mg/L glycine and 100 mg/L myo-inositol were used as growth medium. In this study, three different auxins were used as treatments: 2,4-D (2,4 dichlorophenoxy acetic acid), picloram, and dicamba applied at 6.0 mg/L. The observation was made on the texture of callus formed and the performance of the somatic embryos obtained. The results showed that the texture of callus obtained is compact formed callus and green in color. The best treatment to induce globular somatic embryos is using 6.0 mg/L picloram within eight months. The best treatment to induce globular and torpedo somatic embryos is BA 3.0 mg/L treatment.
Istiana Prihatini, Balai Besar Litbang Bioteknologi Dan Pemuliaan Tanaman Hutan, Aypbc Widyatmoko, Maryatul Qiptiyah, Dodi Frianto, Eka Novriyanti
Jurnal Pemuliaan Tanaman Hutan, Volume 15, pp 65-76; https://doi.org/10.20886/jpth.2021.15.2.65-76

Abstract:
Conservation management strategies for native Sumatran species such as andalas (Morus macroura) are currently being developed through genetic diversity studies. This genetic diversity information will be used to enhance ongoing conservation and regeneration programs and to control logging of this high value plant species. The aims of this initial study were to develop a method for extracting DNA from andalas cambium, to select polymorphic RAPD primers and to study genetic diversity using selected RAPD primers. DNA was successfully extracted using the CTAB method from all 32 cambium samples collected from Jambi and West Sumatra. Eighteen RAPD primers were screened and six polymorphic primers selected (OPO-6, OPY-6, OPY15, OPW-1, OPW-3 and OPW-4). RAPD analysis using the six selected primers resulted in 52 polymorphic loci and the value of genetic diversity within the population (Hs) of 0.308 and the value of genetic diversity among populations (DST) of 0.091. The highest genetic diversity was observed in Batipuah, Tanah Datar (0.351) and the lowest was observed in Halaban, Lima Puluh Kota (0.205). A test with AMOVA shows that the genetic diversity between individuals is greater than the diversity between populations. The dendogram based on the genetic distance between populations shows that the andalas population in West Sumatra is separated into two groups and both are separated from the Jambi population. The results of this study can be used as a basis for further research on several other andalas populations as well as the basic information for conservation strategies of andalas.
Istiana Prihatini, Balai Besar Litbang Bioteknologi Dan Pemuliaan Tanaman Hutan, Dodi Frianto, Eka Novriyanti, Ilg Nurtjahjaningsih, Aypbc Widyatmoko
Jurnal Pemuliaan Tanaman Hutan, Volume 15, pp 95-104; https://doi.org/10.20886/jpth.2021.15.95-104

Abstract:
The natural population of Taxus sumatrana in several regions in southern Asia including Indonesia has experienced a decline in number. In Sumatra, the population with very few trees was found in Gunung Kerinci and Gunung Tujuh, Kerinci Regency, Jambi. Conservation efforts to protect the extinction of this species are being carried out, one of which is by exploring seeds from natural populations to be planted ex situ. Information on the genetic diversity of Taxus is needed to determine a conservation strategy for this species. This researchwas conducted to obtain information on genetic diversity of Taxus sumatrana. Ten RAPD markers were used to analyze the genetic diversity of 44 individuals from two natural populations, namely Gunung Tujuh and Gunung Kerinci. These markers resulted in 104 loci which were used in the RAPD data analysis using GenAlex ver.6.5 program. The results of the data analysis showed that heterozigosity which showed the level of genetic diversity of the populations of the two populations observed was in moderate level and similar, namely 0.33±0.14 (Gunung Tujuh) and 0.35±0.14 (Gunung Kerinci). The genetic distance between the two populations was also in a moderate value (0.097) and the similarities in genetic structure based on the RAPD markers were showinggenetic variety in moderate level (0.908). The results indicate that the two populations, although geographically close have moderate level of genetic structure. Genetic conservation efforts for future Taxus species could increase the number of individuals from both locations and separate them as two distinct populations.
Siti Wahyuningsih, Balai Penelitian Teknologi Serat Tanaman Hutan, Syofia Rahmayanti
Jurnal Pemuliaan Tanaman Hutan, Volume 15, pp 105-113; https://doi.org/10.20886/jpth.2021.15.2.105-113

Abstract:
Geronggang (Cratoxylum arborescens) is able to adapt to ex-burned peatland. However, the viability of germination seed in peat soil is low. Composted sludge of paper and/or pulp wastewater treatment can provide nutrients to plants. This study compares the vegetative growth of geronggang seedlings after the addition of a composted sludge of pulp, and pulp and paper in peat soil after six months of planting. The sludge was air-dried to gain a water content of 60-70%. Decomposers namely Penicillium citrinum and P. oxalicum with a density of 107(spores.ml-1) were added to the sludge. The composting was held for a month. The sludge compost was added to the seedlings with a dosage equal to 2, 4, 6, 8, 10, 12, 14 and 16 (ton.ha-1). Height and diameter increment of the seedlings were recorded for six months and the data was analyzed using ANOVA. Application of pulp sludge compost at a dosage equal to 16 (ton.ha-1) resulted in a better height increment than other treatments after six months of planting. The addition of pulp sludge compost at a dosage equal to 12 (ton.ha) orpulp and paper sludge compost at a dosage equal to 8 (ton.ha-1) resulted in seedlings with a better diameterincrement. However, those dosages which result in a better height and diameter increment of the seedlings were not statistically different from some treatments. In conclusion, pulp sludge compost is better than pulp and paper sludge compost to enhance the growth of geronggang seedlings in peat soil.
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