Results in Journal Jurnal Natur Indonesia: 195
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Jurnal Natur Indonesia, Volume 10, pp 36-41; doi:10.31258/jnat.10.1.36-41
The leaves of Erythrina variegata (Leguminosae) used tradisional plant of an antimalarial. In the course of our continuing search for novel an antimalarial compound from Erythrina plants, the methanol extract of the leaves ofE. variegata showed significant antimalarial activity in vitro toward Plasmodium falciparum in vitro using the lactate dehydrogenase (LDH) method. The methanol extract of the leaves of E. variegata showed against bothstrains of parasite with IC50of 6.8 ?g/ml against K1 and > 60 ?g/ml against 3D7, respectively. The methanol extract of the leaves of E. variegata was separated by using bioassay-guide fractionation. The n-buthanol fraction yieldedthe most activity, exhibiting equipotency against both strains of parasite with IC50of 5.1 ?g/ml against K1 and 13.5 ?g/ml against 3D7, respectively. Furthermore, by using the antimalarial activity to follow separation, the n-buthanol fraction was separated by combination of column chromatography to yield an active compound. The active compound showed antimalarial activity against both strains of parasite used with IC50 of 4.3 ?g/ml against K1 and 23.5 ?g/ml against 3D7, respectively. Its inhibition of the resistant strain (K1) was also much better compared to its inhibition of the sensitive strain (3D7), indicated that the leaves of E. variegata to be potential as antimalarial agents, but its lower potency compared to artemisinin and chloroquin.
Jurnal Natur Indonesia, Volume 18, pp 20-30; doi:10.31258/jnat.18.1.20-30
Body epidermis has an important health function to prevent body dehydration. This research aims to compare Transepidermal Water Loss (TEWL) values between durian seed oil and tengkawang oil. Skin that had been applied with tengkawang oil and durian seed oil lotion were tested for irritation and TEWL values. The tengkawang oil had an acid number of 17 g/mol and contained 8.54 % free fatty acid, while the durian seed oil had an acid number of 6.34 g/mol and contained 2.89 % free fatty acid. Irritation testing was done using lotion containing 100 % of each oil. TEWL analysis on skin applied with the tengkawang oil lotion after 1 hour showed that the lotion could reduce epidermal water evaporation by 35.23 % compared to the skin condition before treatment. Epidermal water evaporation reduction at the second, third and fourth hours after the tengkawang oil lotion application was 31.33 %, 33.93 % and 27.9 %, respectively. TEWL analysis on skin applied with the durian seed oil lotion after 1 hour showed that the lotion could reduce epidermal water evaporation by 11.34 % compared to the skin condition before treatment. Epidermal water evaporation was reduced 14.64 %, 10.53 % and 2.99 % at the second, third and fourth hours after application of the durian seed oil. Based on these results, it can be concluded that tengkawang oil gives better TEWL values compared to durian seed oil.
Jurnal Natur Indonesia, Volume 12, pp 44-48; doi:10.31258/jnat.12.1.44-48
Javasom Talinum paniculatum Jacq. (Gaertn.) is one of popular vegetable plants with potential medicinal properties.The plant root is often used as a substitute for ginseng (Panax ginseng L.), and the leaves are used as a vegetableand as a substitute for purslane (Portulaca olaraeae L.). An effort to improve genetic quality of the plant was madethrough induced mutation with ethyl methane sulphonate (EMS). A variety of EMS dosages (0, 0.3%, 0.6%, 0.9%,1.2%, 1.5% dan 1.8%) were applied to javasom seeds to induce mutation for 24 hours at room temperature. Selectedfive putative mutant genotypes of the third generation after mutagen treatment (M3) was analyzed using Ramdomamplified polymorphic DNA (RAD) marker. Twenty RAPD primers generated 185 scorable bands with 78.92% ofthem were polymorphic. Size of the bands varied from 200bp to 2.5kbp. Clustering analysis was performed basedon RAPD profiles using the UPGMA method. The range of genetic distance among individual genotypes was fromfrom 0.31 to 0.58, while genetic variance was 0.29 + 0.17. The five genotypes were proof to be solid mutants.
Jurnal Natur Indonesia, Volume 17, pp 13-21; doi:10.31258/jnat.17.1.13-21
Blood cancer (leukemia) is a cancer that occurs due to malignancy of blood cells. Treatment of leukemia generally causes damage to normal cells. Therefore, it needs a drug that has the effect of repairing cell damage and the ability to boost immunity of normal such as taurine and Gynura leaves which are expected to have anticancer and antioxidant properties. The purpose of this study was to investigate the effect of taurine and dewa leaf extract on blood tissues induced by benzo [α] pyrene in vivo, by looking at changes in body weight, the number of red blood cells (erythrocytes), the total number and differentiation of white blood cells (leukocytes) in mice (Mus musculus). Data were analyzed by One Way ANOVA test followed by LSD at 5% significance level. The treatment groups were the group I was given 0.2 mL of corn oil (negative control), group II (given benzo [α] pyrene as a positive control, Group III (given taurine 7.8 mg/BW/day starting from day 1 to 15 before the induction of benzo [α] pyrene until the end of the study), Group IV was given benzo [α] pyrene, then were given taurine 7.8 mg/BW/day were given 2 times a day, as well as the V group was given benzo [α] pyrene, then given a dose of Gynura leaf extract 277.8 mg /BW/day. The results showed taurine and Gynura leaf extract were able to obstruct leukemia by increasing body weight, erythrocyte, leukocyte, and the number of leukocyte differentiation which becomes normal again. In conclusion taurine has better ability for therapoitic than Gynura leaf extact against blood cells induced by benzo[α]piren
Jurnal Natur Indonesia, Volume 10, pp 66-69; doi:10.31258/jnat.10.2.66-69
An isolate of green algae Dunaliella sp. from BBAP Jepara is usually used as a source for carotenoid supplementfor marine animal cultivation in the local area. In order to improve carotenoid production especially detection ofbiosynthetic pathway from the organisms investigated in this study, the main purpose of this study is characterizingDunaliella sp. based on it’s microbiological and ecophysiological characters. The research was done by characterizethe growth, the cell and colonies microbiologically, total pigment production, and also characterize all of theecophysiological factors affecting the algal growth and survival. The results of this research showed that Dunaliellasp. posseses typical characteristic of green eucaryote alga, in their growth and ecological condition. The extremecharacters which was toleration ability to high salinity environment of was used to conclude Dunaliella sp. asDunaliella salina.
Jurnal Natur Indonesia, Volume 13, pp 105-111; doi:10.31258/jnat.13.2.105-111
The objectives of the study are to describe the iron content of seagrass area ecosystem and its relation with nature of physico-chemical territorial water. The sampling of seagrass was conducted in purposive sampling technique for every status of the field and performed in three spots of the seagrass ecosystem region with reference to the depth of water and the current direction. Based on statistical tests of factorial and variance analysis indicate that the quality of physico-chemical territorial water in the coastal region of Donggala regency still very good, relatively. The result also showed, The Demand Oxygen and the turbidity gave a positive effect to Fe- seagrass improvement in its various association. Besides, the limiting factor for the low of Fe-seagrass is the height of salinity and water temperature. If the results were combined with another micro and macro mineral analysis will to become the basic for estimating the status of seagrass fertility.
Jurnal Natur Indonesia, Volume 13, pp 200-206; doi:10.31258/jnat.13.3.200-206
Antibiotic resistance is an ability of bacteria to hold the antibiotic effect. It was reported that there is a human-patogen bacteria that resistance to one or more classes of antibiotic. It become a problem on medical world. Tosolve those problems, it is necessary to search the new antibiotic compounds that more effective and efficient tosolve the problem of Multi Drug Resistance (MDR). The secondary metabolite-producing marine invertebrates andsymbiont microorganisms, have prospect as an antibiotic. The symbiont microorganisms may produce thesecondary metabolite similar to their host. The aims of the reseach were to determinate of gastropods symbiontbacteria that capable of producing Antibacterial MDR (Multi Drugs Resistant) Compound. Sample of Molusc werecollected from Ternate (Molucas) islands. Isolation of symbiotic bacteria, screening for bacteria which producingsecondary metabolites as anti-MDR bacteria, antibacterial test, isolation of clinical pathogenic bacteria (MDR),conducting anti-bacterial sensitivity test, sensitivity test for antibacterial, DNA exctraction, DNA amplificationbased on PCR method, DNA sequencing. Result of 16S r-DNA sequence was then analyzed and edited usingGENETYX program and followed by 16S rDNA sequence analysis. The result showed that 17 strains were isolatedfrom gastropods Stramonita armigera. Antibacterial assays showed that TSA 8.7 isolate have ability to inhibitPseudomonas sp., Escherichia coli dan Enterobacter sp. the molecular analyses showed that isolate TSA 8.7closed by related to Vibrio sp. Strain JZDN1, with 98% of homology. Based on this experimental result, it could beconcluded that gastropods-symbiont bacterium Stramonita armigera capable of producing antibacterial compoundagainst strain Multi Drug Resistant (MDR). There is 11 isolates of gastropods-symbiont bacteria Stramonita armigerathat have an antibacterial MDR activity.
Jurnal Natur Indonesia, Volume 11, pp 83-88; doi:10.31258/jnat.11.2.83-88
A study on the flower biology on keladi tikus (T. flageliforme) was conducted to uncover if its spathe inhibitspollination from external pollen resources and causes very low fruit set. The spathe box closely envelopes thesexually vital properties of its inflorenscence. Observation was carried out on some plant clump grown on someuniform potting. A hole was made with scalpel about 4 x 5 mm on a side of the spathe to break its isolation. Somenearer flowers in same clump were left without treatment at all as experimental control. Parameter observed waspresence or absence of seeded fruit setting on spadix. The study result showed that almost all treated flowerspadix per potting, set seeded fruits significantly higher (63, 69 ± 37, 64) than that of controls (3, 57 ± 9, 45) so it isa great possibility that spathe can inhibit partially fruit set. Flower structure and its reproductive phenology werealso described.
Jurnal Natur Indonesia, Volume 13, pp 59-62; doi:10.31258/jnat.13.1.59-62
A triterpenoid derivative compound, oleane-12-en-2,3,23-triol-28-oat, from chloroform fraction of root wood ofPterospermum subpeltatum C. B. Rob (Sterculiaceae) was identified using IR, 1H dan 13C NMR data. The compoundhas shown toxicity against Artemia salina, LC50 of 46.06 μg/mL, and antibacterial potential upon Shigella boydii andStaphylococcus aureus with inhibition zones of 8.3 and 9.8 mm respectively.
Jurnal Natur Indonesia, Volume 13, pp 207-213; doi:10.31258/jnat.13.3.207-213
Cooking banana (Musa acuminata) cv. Kepok is the most susceptible to Blood disease bacterium (BDB) infection.From previous study revealed the best isolate indigenous Arbuscular Mycorrhiza Fungi-Pasar Usang 10 (PU10-Glomus sp 1) could induce cv.Kepok resistance to BDB in green house and field experiment. The AMF could changethe phenolic compound in root plant. This objectives were to measure the root phenolic compound and bioassayto BDB. The 50 grams fresh inoculant PU10-Glomus sp 1 were applicated to banana root plants 60 days old with 6levels time course: 12; 24; 36; 48; 72; 92 hours and control (without PU10-Glomus sp 1). The root methanolicextraction followed to Echeverri et al., (2002) methode with vacuum concentration of the filtrate and partitioninginto ethyl acetate revealed the presence of an antibacterial compound as detected by TLC (Thin LayerChromatography), assay phenolic contained by Spectrofotometer UV-Vis 1700. PharmaSpec. Shimadzu andbioassay using BDB. Nine antibacterial compounds rose from root banana seedling colonized by PU10-Glomus sp1 in 12 hours after applicated (haa) ; 24; 36 and 48 haa. They were with Rf values of 0.16; 0.17; 0.19; 0.26; 0.32; 0.37;0.71; 0.80 and 0.83 on silica plates run in hexane:ethyl acetate (1:2 v/v) and control contained only 0.05 and 0.28.These compounds produced fluorescens which was bright yellow green spots and purple and have antimicbrobialproperties to BDB.