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Results in Journal JURNAL KAJIAN VETERINER: 33

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Yohanes T. R. M. R. Simarmata, Margie P. Mila Meha, I Dewa Made Anom
JURNAL KAJIAN VETERINER, Volume 8, pp 92-101; doi:10.35508/jkv.v8i1.2266

Abstract:
Transmissible venereal tumor is a tumor that generally infects male and female genitalia and transmission occurs due to marriage and licking. On August 7th , 2019 the owner came with complaints of fresh blood dripping continuously from the vulva of Unyil, a local dog, female, approximately 3 years old, brown hair color, with a weight of 10.2 kg. After a physical examination in the perineal area, there is a mass like grapes formed in the vagina. Based on the results of physical examination and haematology test, the dog was diagnosed with transmissible venereal tumor. Handling is done in this case by removing the mass such as grapes formed in the vagina through surgery.
Deswandi W. S. Berri, Julianty Almet, Diana Agustiani Wuri
JURNAL KAJIAN VETERINER, Volume 8, pp 54-68; doi:10.35508/jkv.v8i1.2282

Abstract:
Dengue hemorrhagic fever (DHF) is a disease that is found in some tropical and subtropical regions. This disease is caused by dengue virus and is transmitted to humans through the bite of the Aedes aegypti mosquito. the solution taken in controlling DHF is to break the life cycle of the Aedes aegypti mosquito. Vector control is generally carried out using synthetic larvicides, namely abate / temefos, but the use of abate can cause residues, environmental pollution, poisoning and resistance of the eradicated vectors so that natural larvasides from plants are needed namely temulawak rhizome (Curcuma xanthorrhiza Roxb.) for vector control. The purpose of this study was to determine whether temulawak rhizome extract was effective or not in killing Aedes aegypti larvae. This research method includes larva collection, identification and maintenance of mosquitoes, determining sample size, making extracts and testing effectiveness. This study used a control and experiment group with 3 repetitions in the minutes to 15, 30, 45, 60 and 1440 (24 Hours). The control group was positive control using abate and negative control using aquades while the eksperiment group used extract of temulawak rhizome (Curcuma xanthorrhiza Roxb.) with concentrations of 0.6%, 0.8%, 1%, 1.2% and 1.5%. The results of this study indicate that the temulawak rhizome extract (Curcuma xanthorrhiza Roxb.) effective as larvicides because at the lowest concentration 0.6% can kill 100% Aedes aegypti larvae.
Yohanes T. R. M. R. Simarmata, Anita Kartini Lakapu, I Dewa Made Anom
JURNAL KAJIAN VETERINER, Volume 8, pp 81-91; doi:10.35508/jkv.v8i1.2265

Abstract:
Pyometra in dogs is a condition of the accumulation of purulent material in the uterus of female dogs caused by hormonal imbalances resulting in bacterial infections and excess fluid production in the uterus. A female Golden Retriever named Merry, age 4 years old, having her address at Jln. Raya Dharmasaba Denpasar with complaints of a limp dog, anorexia, issued a thick reddish-colored discharge from the genitals and smelly. Based on the results of systematic clinical examination and the results obtained that the temperature of the dog is 38.1 ℃ (normal), pulsus frequency 120x/minute and the frequency of breathing 40x/ minute. At the time of inspection and palpation of the large abdomen, the nipple comes out. In a complete blood test conducted at Anom Animal Clinic showing the results of Leucosytosis (an increase in white blood cells) which indicates the incidence of bacterial infection, the dog is diagnosed with pyometra after being supported also by X-ray examination results showing radiopaque on the abdomen and ultrasound results showing the uterus anechoic. Dogs treated by surgery to remove the ovary and uterus (ovaryohysterectomy) and continued administration of antibiotics and analgesics. On the 9thday after surgery the incision wound had dried, the skin fused well, and was no longer removing the discharge from the genitals.
Jois Moriani Jacob, Yanse Yanne Rumlaklak
JURNAL KAJIAN VETERINER, Volume 8, pp 43-53; doi:10.35508/jkv.v8i1.1927

Abstract:
Brucea javanica (L) Merr is a shrub that has various types of secondary metabolites that have pharmacologic effects. Various studies on this plant in Indonesia have been conducted, but research on secondary metabolites that have the potential as herbal medicines from this plant originating from the island of Timor has never been done. Therefore, this study aims to evaluate secondary metabolites found in Brucea javanica (L) Merr which originates from Timor Island. Samples in the form of roots, stems, leaves, seeds, and flowers of plants are sent to the Indonesian Institute of Sciences (LIPI) to identify these plant species. Plant determination is carried out under the determination procedure in the LIPI ethnobotany laboratory. The plant determination test results showed that the plant samples sent were Brucea javanica (L) Merr. While the phytochemical tests were carried out at the Laboratory of Chemistry at the Faculty of Science and Engineering of the University of Nusa Cendana to identify secondary metabolites from the Brucea javanica (L) Merr plant according to the phytochemical test standards in the Undana Chemistry laboratory. Phytochemical tests show that there are 4 secondary metabolites found in Brucea javanica (L) Merr namely Alkaloids (+), Tannins (+++), Saponins (+), and Triterpenoids (+++) while secondary metabolites such as Flavonoids and Steroids are not contained in this plant.
Mirnawati B Sudarwanto, Susi Soviana, Herwin Pisestyani
JURNAL KAJIAN VETERINER, Volume 8, pp 24-33; doi:10.35508/jkv.v8i1.2230

Abstract:
Milk is an animal food source that plays a role in human life because it contains various nutritional components. Indonesia’s level of milk consumption is still relatively low. As one of an attempt to increase milk consumption, various kinds of processed milk products with a more modish aroma and form were made. However, not all of the quality of the processed milk products sold can be guaranteed. The aim of this study is to determine the quality of milk samples obtained from milk retails located around the students settlement area of IPB University’s Dramaga and Cilibende campus. The total of retails around campus obtained from the survey were 13, so 39 samples were bought. Milk samples taken were plain milk without an addition of sugar, flavor variants or ice cubes. The results obtained by the average value of density, content of fat, protein, lactose, dry matter, non-fat dry matter and pH of milk samples in Dramaga region are 1.0190 g/mL, 2.66%, 2.02%, 3.03%, 8.18%, 5.52% and 6.89. Milk samples in Cilibende region are 1.026 g/mL, 3.80%, 2.75%, 4.13%, 11.34%, 7.54% and 6.54. Based on pasteurization and sterilization perfectness test, it was discovered that all samples were pasteurized milk, even as much as 30.8% were sterilized milk. From this study, it can be concluded that the ready-to-drink milk sold in retails around the students settlements of IPB University has poor quality because it does not meet the minimum standard requirements of SNI No. 01-3951-1995 about Pasteurized Milk.
Ulfa Apriliana Annisa, Mirnawati B Sudarwanto, Susi Soviana, Herwin Pisestyani
JURNAL KAJIAN VETERINER, Volume 8, pp 34-42; doi:10.35508/jkv.v8i1.2229

Abstract:
Nowadays, milk is one of the favorite drinks for Indonesian people. This relates to the awareness of society about healthy food is increasing. At the same time, there are many milk retail that are appearing. The purpose of this study was to detect the presence and level of contamination of Salmonella sp. in processed milk from milk retail around the students settlement area of ​​IPB University. In Indonesia the standard for identifying Salmonella sp. refer to Indonesian National Standard 2897: 2008. This method consists of 5 stages: pre-enrichment, enrichment, selective media planting, confirmation based on biochemical and serological tests. Milk samples in this study came from 14 milk retail in the students settlement area of the IPB University. The milk samples were taken are plain milk (milk that has not added flavor (flavorings), sugar, and ice). The selection of milk retail is taken within a radius of 2 km from the midpoint of the IPB campus in the Dramaga and Cilibende areas. Based on the results of this study, it was found that all milk samples examined showed negative results which means no Salmonella sp. contamination was found. From this study it can be concluded that the quality of processed milk sold in milk retail around the student settlement area of IPB University is good.
Mario H.Cantona, Maxs Urias Ebenheizer Sanam, Tri Utami, Tarsisius Considus Tophianong, Antin Y.N Widi
JURNAL KAJIAN VETERINER, Volume 8, pp 69-80; doi:10.35508/jkv.v8i1.2292

Abstract:
Controlling Septicemia epizooticae (SE) through vaccination program has been undertaken in Kupang City. However, numbers of fatal cases are still being reported. The purpose of this study is to measure the antibody titer of Bali cattle after SE vaccination, and to determine the effect of age and sex on antibody titers. The 50 serum samples of SE vaccinated Bali cattle were taken from Alak Sub-district (26 samples) and Maulafa Sub-district (24 samples). The selection of sub-districts in Kupang City was taken in a simple random manner. Those serum samples were examined using the indirect enzyme-linked immunosorbent assay (ELISA) method. Antibody titers against SE is declared to be protective when the antibody titer is above 88 ELISA Unit (EU). Results indicated that average value of cattle antibody titer after the SE vaccination was able to trigger a protective antibody response (> 70 EU), meanwhile ONE WAY ANOVA analysis results showed that there is no significant effect (P> 0.05) of cattle age towards antibody titers. In the same way, the paired t test results did not indicate a difference in the value of antibody titers against the sex of the Bali cattle.
Selviani Trivoningsi Dangur, Novalino H. G. Kallau, Diana Agustiani Wuri
JURNAL KAJIAN VETERINER, Volume 8, pp 1-23; doi:10.35508/jkv.v8i1.2241

Abstract:
Pork is one of the most consumption types of meat in the East Nusa Tenggara region.. Moringa leaf are one part of the plant which is known to have antimicrobial compounds.The aimof this research was to determine the influence of Moringa leaf infusion as a natural preservative to the quality of pork.This research is an experimental laboratory research and used a total of 48 samples of thigh pork (Biceps femoris). This research used a completely randomized design with factorial pattern. The first factor was concentration of infusion Moringa leaf consist of 0% (K0), 5% (K1), 10% (K2), and 15% (K3) and the second factor was time of storage consist of 0,6, 12, and 18 hours with 3 replications. The parameters of porkquality that have been examined: color, texture, odor, value in the Eber test, pH value, and total plate count (TPC) value.The results showed that the addition of Moringa leaf infusion change the color and odor. The Eber test shows the K3 group can last up to 18 hours. There was no significant effect of infusion concentration (P> 0.05) on the pH value and there was a very significant effect on the time of storage (P
Aven B. Oematan, Gerson Y. I. Sakan, Devi Y. J. A. Moenek, Bernadette B. Koten, Victor Lenda
Published: 14 February 2020
JURNAL KAJIAN VETERINER, Volume 7, pp 101-106; doi:10.35508/jkv.v7i2.1980

Abstract:
Tujuan Penelitian ini dilakukan untuk mengetahui keragaman jenis, pola aktivitas lalat dan hubungannya dikaitkan dengan suhu, musim, serta jenis kelamin. Penelitian di peternakan sapi semi ektensif kelurahan tuatuka Kecamatan Kupang Timur, Kabupaten Kupang, Nusa Tenggara Timur. Sampel lalat dikoleksi menggunakan New Zealand 1 trap di peternakan sapi. Perangkap dipasang secara random langsung di lokasi padang penggembalaan, sedangkan di kandang, perangkap ditempatkan pada jarak 3-5 meter dari pagar pembatas kandang. Perangkap dipasang pada jarak kurang lebih 10-15 cm di atas permukaan tanah. Pengumpulan sampel lalat dilakukan dalam interval waktu satu jam sejak pemasangan, yaitu pukul 09;00, 10;00, 11;00, 12;00, 14;00 dan pukul 15;00. Lalat dikumpulkan dan dieuthanasia dengan alkohol 70%, kemudian di identifikasi menurut spesies dengan kunci identifikasi menurut Scott et al (1967) dan Dodge (1967), selanjutnya data dianalisa secara deskriptif.
Published: 14 February 2020
JURNAL KAJIAN VETERINER, Volume 7, pp 107-113; doi:10.35508/jkv.v7i2.1981

Abstract:
Piometra adalah gangguan reproduksi yang terjadi pada anjing berusia muda dan sangat umum pada anjing berusia lebih dari 8 tahun. Pada tanggal 14 Februari 2019 seekor anjing Golden Retriever berjenis kelamin betina, berusia 8 tahun bernama Chelsea dibawa pemilik ke tempat Praktik Dokter Hewan Bersama (PDHB) Grace Vet Care. Riwayat kasus anjing Chelsea 1 x beranak pada tahun 2016 dengan kelahiran eutokia/normal, tidak ada riwayat operasi ovariahisterektomi dan gejala klinis berupa discharge berwarna kecoklatan dengan aroma amis menyengat dari vulva, nafsu makan menghilang, muntah, frekuensi pernafasan 92x/menit, frekuensi pulsus/nadi 80 x/menit dan suhu tubuh 38.90C. Pernafasan dengan ritme cepat dan dalam (terengah-engah/panting), palpasi pada kelenjar parotidea kiri mengalami pembengkakan. Auskultasi jantung terdengar cepat dan kuat. Palpasi pada ventrolateral abdomen mengalami distensi tanpa fluktuasi. Hasil pemeriksaan darah menunjukkan terjadinya trombositopenia, neutropenia, limfositosis, azotemia, hiperproteinemia, hiperglobulinemia dengan rasio albumin:globulin normal. Berdasarkan hasil pemeriksaan USG transabdominal terlihat pembesaran ukuran uterus dan penurunan echogenitas (anechoic) pada lumen uterus. Berdasarkan riwayat reproduksi dan operasi, observasi klinis, pemeriksaan klinis dan organ reproduksi, hematologi analisis, palpasi abdomen dan diagnosa pencitraan ultrasonografi (USG) anjing Chelsea terdiagnosa piometra terbuka.
Devi Moenek, Aven B. Oematan, Novianti N. Toelle
Published: 14 February 2020
JURNAL KAJIAN VETERINER, Volume 7, pp 114-120; doi:10.35508/jkv.v7i2.1983

Abstract:
Penelitian ini bertujuan untuk mengetahui keragaman jenis endoparasit gastrointestinal dan pengaruhnya terhadap profil darah ayam kampung yang meliputi jumlah eritrosit, kadar hemoglobin, Packed cell volume, dan jumlah total leukosit. Pengambilan sampel feses dan darah ayam kampung dilapangan sedangkan pemeriksaan dan identifkasi endoparasit pada feses ayam kampung serta pemeriksaan darah di laboratorium Kesehatan Hewan Program Studi Kesehatan Hewan Politani Kupang. Peubah yang diamati adalah morfologi atau ciri-ciri endoparasit dan gambaran darah terutama jumlah eritrosit, kadar hemoglobin, Packed cell volume, dan jumlah total leukosit Seluruh sampel yang terkumpul diidentifikasi menggunakan mikroskop dibantu dengan panduan kunci identifikasi dan didokumentasikan menggunakan kamera digital, dan data gambaran darah yang diperoleh akan disesuakan dengan gambaran darah normal kemudian semua data yang diperoleh, diolah secara deskriptif. Penelitian ini akan dilaksanakan didaerah Kecamatan Amarasi Kabupaten Kupang. Dari pemeriksaan ditemukan telur cacing Ascaridia galli untuk semua sampel feses dan pemeriksaan darah menunjukkan bahwa jumlah eritrosit 3.782 X 10^6/µL, kadar hemoglobin 12.16 g/L, PCV 43.18%, dan jumlah total leukosit 112.5 X 10^3/µL.
, Frans Umbu Datta, Annytha Ina Rohi Detha, Nemay A. Ndaong, Maria Moi
Published: 14 February 2020
JURNAL KAJIAN VETERINER, Volume 7, pp 128-133; doi:10.35508/jkv.v7i2.1985

Abstract:
Penelitian ini bertujuan untuk mengetahui gambaran sel vagina kambing kacang lokal (capra aegagrus) selama satu siklus estrus. Sampel diambil dari 10 ekor kambing kacang betina produktif dengan kisaran umur 12-18 bulan . Sampel di buat preparat ulas dengan motode roll, dan diwarnai dengan Giemsa ,dilakukan dengan jarak interval 24 jam diulang selama 20 hari. Hasil menunjukkan persebaran sel-sel kornifikasi, superfisial, intermediet ,sel parabasal dan leukosit selama 20 hari pengamatan. Kesimpulannya, panjang siklus estrus kambing kacang lokal (capra aegagrus) 17-20 hari dengan panjang tahapan proestrus 1-2 hari, estrus 1-2 hari, metestrus 3-5 hari dan diestrus 12-15 hari.
, Dyah Widhowati, Nurul Hidayah
Published: 14 February 2020
JURNAL KAJIAN VETERINER, Volume 7, pp 93-100; doi:10.35508/jkv.v7i2.1978

Abstract:
The aimed of this study was to identify antibiotic Resistance of Escherichia coli isolated from Shrimp. There were 20 samples taken and isolated on surface of Eosin Methilen Blue Agar. E.coli produced metalic green colonies. Samples identified as E.coli based on macroscopic features and morphology of colonies, microscopic examination with gram staining and biochemical tests. The antibiotic sensitivity was determined through a standard antimicrobial disk diffusion test. The results of this study showed that 9 samples were bacteria of the spesies E. coli. Based on the results of antibiotic sensitivity test, it was found that 100 % of E. coli isolates were resistant to Amoxycillin 20 µg and 22,2 % of isolates are resistant to ampicillin 10 µg. Antibiotics Tetracycline 30 µg and Chloramphenicol 30 µg were 100 % sensitive. Gentamicin 10 µg showed that 77,8 % sensitive and 22,2 % intermediet. The precence of antibiotic resistance of betalaktam antibiotics makes it especially important to monitor antimicrobial susceptibility and mechanisms of resistance of E.coli isolated from shrimp, because new mechanisms of resistance occurring in animals may enter the food chain and its be transferred to the human. This importance of cooperation between sectors in order to monitor antimicrobial resistance.
, Diana A. Wuri, Annytha Ina Rohi Detha, Dionesia A. Mogi
Published: 14 February 2020
JURNAL KAJIAN VETERINER, Volume 7, pp 121-127; doi:10.35508/jkv.v7i2.1984

Abstract:
Pengendalian nyamuk yang berperan sebagai vektor merupakan tindakan terpenting dalam penanggulangan penyakit seperti filariasis.Filariasis merupakan penyakit menular yang disebabkan oleh cacing filaria yang menyerang saluran dan kelen­jar getah bening yang ditularkan oleh berba­gai jenis nyamuk. Kasus filariasis di Kabupaten Sikka tahun 2015- 2017 setiap tahunnya meningkat. Penggunaan insektisida menjadi pilihan utama masyarakat dalam pengendalian vektor nyamuk. Insektisida yang digunakan secara terus-menerus dapat menyebabkan nyamuk menjadi resisten. Penelitian ini bertujuan untuk mengetahui status resistensi vektor filariasis terhadap insektisida permethrin 0,75% di Kabupaten Sikka tahun 2018. Penelitian ini diawali dengan survei lokasi dan pengambilan sampel di Kabupaten Sikka dan dilanjutkan dengan pemeliharaan nyamuk serta uji resistensi. Bahan yang digunakan dalam penelitian ini adalah nyamuk Aedes sp. dan Anopheles sp., impregnated paper permethrin 0,75%. Hasil uji resistensi menggunakan metode susceptibility test dengan impregnated paper permethrin 0,75% adalah kematian nyamuk uji terhadap permethrin adalah 21,3% sehingga disimpulkan bahwa vektor filariasis di Kabupaten Sikka dinyatakan telah resisten tinggi terhadap insektisida permethrin 0,75% karena kematian nyamuk uji
JURNAL KAJIAN VETERINER, Volume 7, pp 85-92; doi:10.35508/jkv.v7i1.1058

Abstract:
The purpose of this study was to isolate and identify the lactic acid bacteria found in Sumba mares milk. This research will be carried out from September to November 2017 at the Veterinary Laboratory of the Livestock Service Office of East Nusa Tenggara Province. The research stages are isolation of lactic acid bacteria from Sumba mares milk by growing lactic acid bacteria on MRS agar media, and identifying lactic acid bacteria with gram staining, catalase test, motility test, and total plate count testing. Based on the results of the study, the presence of lactic acid bacteria originating from Sumba mares milk was characterized by the presence of colonies of lactic acid bacteria from Sumba mares milk which grew on MRS media agar as a selective medium for lactic acid bacteria. Based on the results of testing the characteristics of lactic acid bacteria Sumba mares milk has Gram positive, the negative results in the catalase test are characterized by not forming gas bubbles when the bacteria are added with H2O2. Based on the motility test obtained negative results or bacteria are non-motile and the number of lactic acid bacteria that can be a total picture of lactic acid bacteria that exist in Sumba mares milk in milliliter of milk. The conclusion are lactic acid bacteria isolated from Sumba mares milk has characteristic as Gram positive, basil or stem shaped, negative catalase and non motile, and has a total lactic acid bacteria of 3.5 x 108 cfu/ml (est).
Meity Marviana Laut, Nemay Ndaong, Tri Utami, Maria Junersi, Yovita Bria Seran
JURNAL KAJIAN VETERINER, Volume 7, pp 1-11; doi:10.35508/jkv.v7i1.377

Abstract:
Regenerasi dan pemulihan jaringan luka merupakan proses fisiologi normal tubuh dalam merespon perlukaan untuk mengembalikan integritas dan fungsi normal kulit. Kesembuhan luka dapat dipercepat dengan pemberian obat kimia maupun obat alami. Salep betadine 10% adalah obat kimia yang sering digunakan dalam perawatan luka untuk menstimulasi penyembuhan luka. Sedangkan, tanaman anting – anting (Acalypha indica) diketahui memiliki aktivitas antiinflamasi, antibakteri, antioksidan, dan antiulser. Daun anting – anting telah lama digunakan oleh masyarakat lokal di NTT untuk mengobati luka dan gangguan kulit lainnya pada ternak peliharaannya. Penelitian ini bertujuan untuk mengetahui gambaran makroskopik dan jumlah fibroblas pada kesembuhan luka insisi pada mencit yang diberi terapi salep ekstrak etanol daun anting – anting (EEDAA), sekaligus sebagai bukti empiris fitofarmaka ini bagi masyarakat lokal di NTT. Penelitian ini bersifat eksperimental laboratorik, menggunakan 30 ekor mencit jantan sehat, berumur 3-4 bulan dengan berat badan berkisar 30-40g. Mencit dibagi dalam 5 kelompok yaitu kelompok kontrol negatif (KN) dan positif (KP) yang masing – masing diaplikasikan basis salep vaselin album dan salep betadine 10%; serta tiga kelompok perlakuan yang masing – masing diberikan salep EEDAA konsentrasi 5%, 10% dan 20%. Setiap kelompok dibuat luka sayat pada area dorsum, dengan ukuran panjang 1.5 cm dengan kedalaman 2 mm. Bahan topikal diberikan sebanyak 2 kali/hari. Pengampilan sampel jaringan luka dilakukan pada hari ke-3, ke-7 dan ke-14. Hasil penelitian menunjukkan bahwa pemberian salep EEDAA konsentrasi 10% menunjukkan kesembuhan luka yang lebih cepat dan optimal dibandingkan dengan salep betadine 10%, salep EEDAA 5% dan 20%.
Yulfia Selan, Filphin A. Amalo, Inggrid Trinidad Maha, Antin Y. N. Widi, Cynthia D. Gaina, Beatrix Barut
JURNAL KAJIAN VETERINER, Volume 7, pp 80-84; doi:10.35508/jkv.v7i1.948

Abstract:
Timorese fruit bat(Pteropusvampyrus)is the only fly mammalian with its unique behavior which hanging upside down inspite of its pregnancy. This research is aimed to reveal the morphology of the Timorese fruit bats and the distribution of neutral carbohydrate within this organ. Three uterus samples derived from three different Timorese fruit bats were used in the research.Both macroscopical and microscopical examinations using H&E and PAS methods were applied. Macroscopically, Timorese fruit bats showedsoft reddish white duplex uterus. Meanwhile microscopically, endometrium consisted of epithelial layer and lamina propria and was the place where simple tubular glands located. The epithelial layer comprised of simple cylindric secretory cells and ciliated cells. Neutral carbohydrate distribution was seen within this epithelial layer. Myometrium was a thick circular smooth muscle layer which consisted of smooth muscle separated by collagen and elastic fibre. Perimetrium was a visceral layer and consisted of mesothelial cells.
, Dewi Fesbayati Lestari Djungu, Emerlinda P. Gelalan
JURNAL KAJIAN VETERINER, Volume 7, pp 53-61; doi:10.35508/jkv.v7i1.869

Abstract:
Helminthiasis is an adverse disease. This disease is caused by parasites such as trematodes, cestodes, and nematodes. The search for bioactive compounds from various plants that have anthelmintic activity is carried out by various researchers. Papaya seeds and mindi leaf have been known as a good plants for health. Various studies shown that extracts water, ethanol and chloroform have activity for antimicrobial, antilipidemia, antidiabetic and antiparasitic. In this study the extraction of secondary metabolites from mindi leaf and papaya seeds was carried out for larvicidal activity. From the results of extraction with ethanol, the yields of mindi leaf and papaya seeds were obtained 19.97% and 12.97% respectively. Phytochemical analysis shows that both extracts have the same class of compounds tannins, saponins, phenolhydroquinones, flavonoids, and alkaloids. The difference is in the concentration of tannin and saponin mindi leaf higher than papaya seeds. In testing larvicidal activity, the extract of mindi leaf had a stronger activity compared to papaya seeds. Extract mindi leaf 15% has the strongest activity of killing larvae at ± 25 minutes. This activity is stronger than extract papaya seed 15% which is ± 125 minutes or with albendazole 0.03%, ± 46 minutes
Marthinus Usmany, Pieter Kakisina
JURNAL KAJIAN VETERINER, Volume 7, pp 12-46; doi:10.35508/jkv.v7i1.652

Abstract:
The cuscus is an Australian animal (marsupial) which belongs to the Phalangeridae family and its distribution is limited in eastern Indonesia, Australia and Papua New Guinea. Through IUCN data, cuscus is categorized as endangered species, in CITES it is classified as Appendix II. The population of cuscus is decreasing due to the threat of deforestation, many are hunted for consumption, and are traded illegally. To overcome the above problems, it is necessary to conduct a comprehensive study of the study of cuscus genotypes in the hope that they can assist in future wildlife conservation efforts. The purpose of this study was to molecularly examine the types of living cuscus in captivity in Lumoli Village, West Seram Regency, Maluku. This research begins with the stages of DNA isolation through cuscus tissue. DNA isolation products were amplified in the ATP 8 gene region by the PCR method, sequenced. Data was analyzed using MEGA program version 5.1. The PCR reaction produces 681 bp of product. The results of the analysis obtained 85 different nucleotide sites. The nucleotide sequence of the ATP 8 gene was analyzed using kimura 2 parameters. The construction of the filogram using the neighbor joining method with a bootstrap value 1000 times based on the ATP 8 gene sequence shows the kinship between the four types of cuscus, which produces two branches of Phalanger and Spilocuscus, namely brown cuscus related to white cuscus and related cuscus with spotted cuskus.
, Cynthia D. Gaina, Alan Prima Titong, Crecentia A. Butta, Maria S. B. Bei
JURNAL KAJIAN VETERINER, Volume 7, pp 47-52; doi:10.35508/jkv.v7i1.852

Abstract:
Tujuan dilaksanakan penelitian ini untuk melihat pengaruh metode penyimpanan preservasi yang berbeda terhadap viabilitas spermatozoa semen babi landrace dalam pengencer alami air buah lontar (AL) dengan penambahan kuning telur ayam kampung (KT). Semen diambil dari 5 ekor pejantan landrace yang telah dewasa kelamin. Setelah itu dilakukan pemeriksaan makros-mikros dari semen segar, semen dengan kriteria motilitas , konsentrasi dan abnormalitas spermatozoa berturt-turut: >70%, >200 juta spermatozoa/ml,
, I Wayan Teguh Wibawan, Denny Widaya Lukman, Mirnawati Baharudin Sudarwanto
JURNAL KAJIAN VETERINER, Volume 7, pp 70-79; doi:10.35508/jkv.v7i1.946

Abstract:
The aim of this study was to determine the pattern of the spread of antibiotic-resistant E. coli on pig farms in Kupang City by using mapping analysis. Data on E. coli resistance comes from the results of laboratory analysis and as secondary data for spatial analysis. Spatial analysis uses the nearest neighbour index, convex hull and elementary analysis of disease methods. The results of this study have shown a high prevalence of E. coli (85.4%) with clustered distribution patterns and have a wide spread (10920 ha) in the area in Kupang City. MDR E. coli had a moderate prevalence (57.31%) by forming a group spread pattern and a wide spread (7778 Ha) on pig farms in Kupang City. This result encourages prevention and control efforts by all interested parties so that the rate of spread of resistant E. coli can be reduced.
JURNAL KAJIAN VETERINER, Volume 7, pp 62-69; doi:10.35508/jkv.v7i1.933

Abstract:
Cryptorrchid is the most common congenital defect found in dogs and cats. Cryptorrchid is a failure condition of one or both testicle to descend into the scrotum. On 2nd March 2019 an examination and treatment of two bilateral cryptorrchid’s dogs were done at the Praktik Dokter Hewan Bersama Grace Vetcare. The first patient’s identity is domestic dog, 3 years old, brown hairs, weigh 13 kg called name Iro. The second patient’s identity is domestic dog, 1.5 years old, brown hairs, weigh 15.5 kg called name Jacky. Based on physical examination and ultrasound examination, two dogs were diagnosed Bilateral Inguinal Cryptorrchid. Management treatment of both patients were done by orchiectomy operation. Orchiectomies were done by an open method, which is making incision in the left and right lateral inguinal region of the penis. This operation was done to minimize the risk of sertoli cell tumor (sertolioma). Orchiectomy is the only medical procedure that was recommended for cryptorchid treatment.
, Diana A. Wuri, Annytha Ina Rohi Detha
Published: 17 December 2018
JURNAL KAJIAN VETERINER, Volume 6, pp 58-68; doi:10.35508/jkv.v6i2.744

Abstract:
The aims of this study were to know the influence of education level and type of work against the level of understanding and application hygiene and sanitation processing of chicken meat by housewives in Kupang City. Determination the quantity of samples is done with software Win Episcope® 2.0 with level of confidence 95%, expected prevalence 50%, and accepted error 5%, then total of 220 respondents involved in this study. Samples carried out by simple random sampling to determine the area (RW) that elected per sub district. Assessment the level of understanding and application of sanitary hygiene is performed by summing the score answers of each item contained in the questionnaire. The data were analyzed descriptively and correlation between the characteristics of the respondents determined by Spearman test. The results showed that most housewife with low education level and not working have a good level of understanding (n=81; 73.6%) and application of sanitation hygiene categorized enough (n=65; 59.1%), housewives with low levels of education and work have a good level of understanding (n=77; 70%) and the application of sanitation hygiene categorized enough (n=65; 59.1%). Spearman correlations test show that there were noticeable at low-educated housewife and not working (p
, Filphin A. Amalo, Yulfia N. Selan
Published: 17 December 2018
JURNAL KAJIAN VETERINER, Volume 6, pp 45-57; doi:10.35508/jkv.v6i2.458

Abstract:
The number of commercial pigs in East Nusa Tenggara has grown fast with a population of 1,739,481, and has become more potential. However, the mixed farming model has become one of the factor of potentially high in the transmission of disease-causing pathogenic microorganisms. One of the microorganism is Pasteurella multocida which causes pasteurellosis, has been identified in 25% of slaughtered pigs (Maes et al., 2001). One of the clinical symptom due to pasteurellosis in pigs is the occurrence of bronchopneumonia in pulmo and inflammation in various visceral organs, such as the heart and kidneys. the phenotypic characteristization of this bacteria, will be very helpful in designing a comprehensive prevention and treatment programs of pig pasteurellosis. The aim of the research was to determine the characteristics of P. multocida related to pasteurellosis and recording of the disease in Kupang, NTT. This research also find out the phenotipyc characteristics of P. multocida species from pigs and the possibility of transmission among sensitive species. A total of 30 swine lung samples of pulmo were obtained from slaughterhouse in Kupang to carry out this study. Pulmo taken from slaughtered pigs that showed clinical respiratoric symptoms such as dyspnoea and the presence of serous to mucopurulent nasal exudates, and the specific lesions of gray hepatization in pulmo. The collected samples were then processed for histopathological and microbiological studies. Out of the total 30 sample, 15 samples were found to be suspected for pasteurellosis, and 3 samples were successfully confirmed to be positive for Pasteurella multocida. Varied macroscopic changes showed pathognomonic lesions as multifocal hemorrhage and congestion of the pulmonary lobes. Serous to mucopurulent exudate were found in lumen bronchus. Multi lobes grayed hepatization and multifocal hemorrhage were observed in the pulmo. Histopatologic analysis showed three types of pneumonia that were multifocal suppurative bronchopneumonia with neutrophil infiltration into alveoli and bronchioles; non-suppurative pneumonia as fibrinous bronchopneumonia with severe congestion, and chronic bronchiolitis with infiltration of mononuclear cell and thickening of fibrous tissue on bronchioles. Bacterial culture from the samples showed circular, convex and non hemolytic colony on blood agar base. Gram staining’s showed Gram negative microorganism with coccoid bipolar structure, which are some of the characteristic of the microorganism.. It was concluded that the samples is having P. multocida infection. Although, some isolate on MacConkey showed lactose fermentation and tolerance to bile salts that were not the nature of the microorganism, isolation and identification from other organs needed to be done, for example from the heart and kidneys, are needed.
Desi Biru, Annytha Ina Rohi Detha, Diana A. Wuri
Published: 17 December 2018
JURNAL KAJIAN VETERINER, Volume 6, pp 85-111; doi:10.35508/jkv.v6i2.934

Abstract:
Zoonotic desease transmission can happen in every stage of animal-orign food production, from farm to table. This study is counducted to evaluate the understanding of farmers and animal-origin food product enterprises about zoonotic diseases and their prevention in Kupang City. The study was conducted 6 region with 200 respondents from farmers, 30 respondents from buthcers, 30 respondents from meat distributors, 30 respndents from meat seller and 30 respondents from food industry. The result than analyzed by descriptive analysis and than presented in graphics and tables. The result shows that the understanding of farmers and animal origin food product enterprise in Kupang City about zoonotic diseases is low so that their behavior, in attempt to prevent zoonoses, is not maximal. In farmers, 87,5% of farmers do not have a proper check up for their animal’s health and 30,5% of them still consume meat from sick and dead animals. In butchers, 80% of butchers do not have a hand-washing facility, 86,7% do not have a seperated room for meat and visceral handling, 96,7% do not use a proper foot-wear when working, and 43,3% of them stil use water from the river or from gutters. In meat distributors, 90% of them only use bike as vehicle for the distribution. In meat sellers, 96,7% of meat sellers allow their customer to directly touch the product, 80% do not have a hand-whashing facility and 100% of them do not have a refrigerator as a storaging vacility. In food industry, 90% of them have a good cleaning behavior, wash their hand with flowing water and soap, and have a refrigerator as storaging vacility. It can be conlcuded that food industry is the only stage with good behavior in atempt to prevent zoonotic desease transmission.
Cynthia D Gaina, Nancy D. F. K. Foeh
Published: 17 December 2018
JURNAL KAJIAN VETERINER, Volume 6, pp 38-44; doi:10.35508/jkv.v6i2.376

Abstract:
Penelitian mengenai studi performa umum dan status fisiologis kuda Sumba di dilaksanakan pada tanggal April-September 2018 bertempat di Peternakan kuda sumba, Pulau Sumba, NTT. Tujuan penelitian ini adalah untuk mengetahui performa umum kuda sumba dalam kaitannya dengan status fisiologisnya. Penelitian yang digunakan adalah metode deskriptif yaitu metode yang menggambarkan objek sesuai dengan apa adanya, pengambilan sampel dilakukan selama dua minggu. Dalam penelitian ini peubah yang diamati adalah frekuensi respirasi, frekuensi denyut jantung dan suhu tubuh yang dilakukan pada 30 ekor indukan kuda sumba memiliki riwayat pernah digunakan untuk olahraga kuda pacuan baik kuda dengan jenis kelamin jantan dan betina dan 5 ekor anakan. Hasil dari penelitian tersebut disimpulkan bahwa frekuensi respirasi indukan memiliki nilai rataan 33±6.7 per menit dan anak memiliki nilai rataan 44±5.7 per menit. Frekuensi denyut jantung indukan memiliki nilai rataan 44±8.0 per menit dan 62±2.8 per menit . Suhu tubuh kuda indukan memiliki rataan yaitu 37.5±0.60C dan suhu anak memiliki rataan yaitu 38.2 ± 0.30C. Selain itu, hasil penelitian menunjukkan bahwa kuda indukan memiliki rataan tinggi badan 138.8 ±8.4 cm dan anakan memiliki rataan 106.2 ± 7.1 cm; indukan memiliki rataan panjang badan 139.4 ± 9.0 cm dan anakan rataan 94.4 ± 3.0; indukan memiliki rataan lebar dada 41.3 ±57.1 cm dan anakan memiliki rataan lebar dada 21.2 ± 3.1 cm; indukan memiliki tinggi punggung 133.9 ± 8.8 cm dan anak memiliki rataan tinggi punggung 104.4 ± 8.6 cm dan indukan memiliki panjang bahu 58.5 ± 5.5 cm dan anak memiliki rataan panjang bahu 40.0 ±5.6 cm
, Diana A. Wuri, Antin Y. N. Widi, Edwin K. Ndawa Lu
Published: 17 December 2018
JURNAL KAJIAN VETERINER, Volume 6, pp 69-77; doi:10.35508/jkv.v6i2.851

Abstract:
The aim of this study is to investigate the insectidace resistance of Anopheles sp. to malathion 0.8% in Kota Lama and Maulafa districts. This study used the sample approximately 650 of Anopheles sp. The collected mosquitoes are inserted into the maintenance cage to identify and ensure that the mosquito is truly Anopheles sp. The test was done by using impregnanted paper malathion 0.8% .Amount of 25 mosquitoes were caught using an aspirator and put into a collector tube which had been coated with 0.8% impregnated paper malathion , while other 25 mosquitoes were put into a green tube and had no insecticide paper. The test results were obtained by counting the knocked down mosquitoes every 15 minutes during 2 hours of insecticide exposure and continued for 24 hours of holding time. The test was repeated for 3 times. The results showed that Anopheles sp. in Kota Lama and Maulafa districts, were resistant to Malathion insecticides 0.8% with the percentage of death mosquitoes (Kota Lama: 4,35-28% ; Maulafa: 8-12,5%)(< 90%). So it has a high level of resistance.
, Tarsisius Considus Tophianong
Published: 17 December 2018
JURNAL KAJIAN VETERINER, Volume 6, pp 78-84; doi:10.35508/jkv.v6i2.932

Abstract:
Esophageal obstruction is a condition that is commonly experienced by dogs and causes disruption to the mobilization of food and water to the stomatch. The aim of this study is to provide information on the management of esophageal obstruction in a dog based on the type and location of the obstruction. The material used in this case study is a Labrador retriever dog, female, 2 years old, brownish red and weighing 24 kg. Based on history, clinical symptoms and radiographic examination, the diagnosis of this case was obstruction of a foreign body in intraluminal esophagus. Before treatment, the dog was anesthetized by premedication Atropine sulfate at 0.02 mg/kg body weight sub-cutaneously, and induction of anesthesia through combination injection of Ketamin HCL dose 10 mg/kg body weight and Xylazine dose 2 mg/ kg body weight intramuscularly. Goal treatment that has been done in this case is taking a foreign body in the form of a piece cow bone in intraluminal esophagus through the oral cavity.
Inggrid Trinidad Maha, I Ketut Mudite Adnyane,
JURNAL KAJIAN VETERINER, Volume 6, pp 1-11; doi:10.35508/jkv.v6i1.959

Abstract:
The common palm civet (Paradoxurus hermaphroditus) is one of the mammalian in Indonesia which have anal gland in female and fefemale that located in the lower tail. This research was aimed to describe the morphology of anal gland of common palm civet female. One female of common palm civet was used in this research which observed macroscopic and microscopic.The result showed that common palm civet have a pair of anal gland which hasshaped like ball and located in the anal sacs wall’s in the ventrolateral of anus.Each anal gland has a ostium of anal canal dorsolaterally in the cutaneous zone of anal canal . Microscopic observation showed a well developed sebaceous gland and apocrine sweat gland in the wall of anal sac. Sebaceous and apocrine glands were excreted their product into main ductus to the anal canal.
Erni Ferawati Kadja, J F BaleTherik, Maxs U. E. Sanam
JURNAL KAJIAN VETERINER, Volume 6, pp 38-55; doi:10.35508/jkv.v6i1.2213

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Yanse Yane Rumlaklak, Jois Moriani Jacob, Aven B Oematan
JURNAL KAJIAN VETERINER, Volume 6, pp 12-16; doi:10.35508/jkv.v6i1.960

Abstract:
Babesia sp. is a protozoa that infects dogs through tick bite and can cause babesiosis. The purpose of blood tests in case dogs is to find out the profile of the dog's red blood cells with babesiosis. On clinical examination found many ticks on the dog's body. Dogs show clinical symptoms of dehydration and paleness in the mucosa. Blood collection is done through the anterior antibrachii cephalica vein. Blood is taken and collected in EDTA tubes. Then the examination is done by examining blood parasites, examining erythrocyte values, examining hemoglobin concentration and hematocrit values. Examination of blood parasites was done by making reticulocyte preparations, while examining erythrocyte values, hemoglobin concentration and hematocrit values were performed using a Vet Scan® HM 5TM machine. The results of examination of reticulocyte preparations showed the existence of Babesia sp infestations with a presentation of 0.68%. Erythrocyte values, hemoglobin concentration and hematocrit values were 3.52x106 // µL, 6.90 g / dL and 23.81%. Based on history, physical examination, clinical and laboratory examination, it can be concluded that the Beagle dog named Loh has microcytic hypochromatic anemia.
Marthinus Usmany, Pieter Kakisina
JURNAL KAJIAN VETERINER, Volume 6, pp 17-30; doi:10.35508/jkv.v6i1.961

Abstract:
Cuscus is an Australian animal (marsupial) whose distribution is limited in Indonesia and its population is declining due to threats hunted for consumption, and traded illegally. To overcome the above problems it is necessary to conduct a comprehensive study of the type of feed in the hope of helping the conservation of these animals, especially in captivity. The purpose of this research is to study the types of plants as cuscus that live in captivity of Lumoli Village, West Seram District. This research begins with the observation of the type of kursus that lives in captivity of Lumoli Village. Study of feed type, depiction, and analysis. The results showed 44 species of plants in the village of Lumoli West Seram District, Maluku can be used as cuscus feed in captivity. Very good plant part as Source of Feed is young leaf of 28 species of plant (63,63%), 25 species (56,81%) are fruits, 4 species (9,09%) are flowers, 3 species (6,81 %) is a young shoot. The level of palatability of the cuscus indicates that the brown cuscus and white cuscus correspond to 43 plant species (97.72%) and can not be done 1 plant species (2.27%) (water apple), while the spotted cuscus and the gray magic cuscus 43 (97.72 %) plant types and unlike 1 plant species (2.27%) (Tomi-tomi). The proximate test results showed the highest ash content was available in the gray cuscus (18.95%) and the lowest in brown couscous (11.41%), the highest crude protein content in the gray cuscus (28.01%) and the lowest in white cuscus (23, 64%), crude fat content in cuscus totol (4.92%) and lowest in gray cuscus (2.93%), crude fiber content in gray cuscus (27.99%) and lowest in brown cuscus (21, 68% ). Can be concluded there are 44 types of forest plants that can be consumed either in the form of young leaves, fruit, flowers and young shoots. Further research is needed on additional types of feed for the cuscus in captivity.
Annytha Ina Rohi Detha, Frans Umbu Datta, Elisabet Beribe, Nancy D. F. K. Foeh, Nemay Ndaong
JURNAL KAJIAN VETERINER, Volume 6, pp 31-37; doi:10.35508/jkv.v6i1.1053

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