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Results in Journal Journal of Aquaculture and Fish Health: 129

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Mohammad Subhan, Prawita Anggeni, Sirrul Hayati
Journal of Aquaculture and Fish Health, Volume 10, pp 95-100; doi:10.20473/jafh.v10i1.22222

Abstract:
Marketing is the main problem felt by salt farmers in East Lombok Regency, especially during the harvest season, which has an impact on the conversion of the salt pond land to cultivation land. This research aims to: 1) identify salt marketing channels in East Lombok Regency, 2) develop salt marketing strategy in East Lombok Regency. This research uses qualitative methods and the data will be analyzed by SWOT analysis.Efficient salt marketing occurs when the marketing channels include producers (salt farmers)-cooperatives /micro, small and medium enterprises-consumers, because it can shorten marketing channels and-reduce operational costs. Marketing of salt in East Lombok Regency can be carried out with several strategies, namely: 1) Maximizing the role of cooperatives and micro, small and medium enterprises in salt marketing, 2) Improving the quality of human resources in managing the salt business, 3) Formulating regulations on salt trading, and 4) Providing production technology training to salt farmers and salt micro, small and medium enterprises.
Ishaaq Saputra,
Journal of Aquaculture and Fish Health, Volume 10, pp 56-65; doi:10.20473/jafh.v10i1.20794

Abstract:
Fish farming including freshwater crayfish still has a dependency on the availability of fish meal as the main source of protein in feed. The purpose of this study was to evaluate alternative protein sources including poultry by-products, feather meal, lupine flour, soybean meal and meat, and bone meal for smooth marron (Cherax cainii) freshwater crayfish feed. A total of 150 juvenile marrons (9.09 ± 0.21 g) were kept for 90 days and distributed into 15 rearing tanks consisting of five treatments with three replications. The results indicated that there was no significant difference in the increase in biomass, survival rate, moulting rate (weight), feed efficiency ratio, and feed digestibility (P> 0.05). However, marron juvenile fed with a protein source of poultry by-products meal had the highest specific growth rate (0.31 ± 0.05 g / day). In addition, marron fed with fish meal protein resulted a significant increase in carapace length (P 0.05). Overall, the results of this study indicate that the general growth performance of marron is not affected by the replacement of fish meal in the feed. Alternative protein materials can be used as a protein source to replace fish meal in marron feed so that the use of fish meal can be reduced.
Anggi Novita Dewi,
Journal of Aquaculture and Fish Health, Volume 10, pp 46-55; doi:10.20473/jafh.v10i1.19922

Abstract:
Surabaya as the capital of East Java Province has a tourism attraction based on mangrove ecosystems namely Wonorejo Mangrove Ecotourism. Interest to the growth of loyalty to the destination depends on service quality, motivation, and destination image. Service quality as the heart of marketing and one of the concepts of loyalty success determines the sustainability of a tourist destination in the future. In addition, motivation is also considered as the main driver for traveling. However, this depends on destination image in the minds of tourists. The purpose of this study was to analyze the direct and indirect relationship between service quality and motivation towards destination loyalty, which is mediated by destination image. From 276 online and offline questionnaires distributed to local tourists who visited or visited the Wonorejo Mangrove Ecotourism in the past years, 182 questionnaires were filled in completely. Data analysis used WarpPLS to examine the relationship of influence between variables. The results showed that service quality and motivation had a positive and significant effect on destination loyalty directly or mediated by destination image. Destination loyalty can be effectively increased by encouraging visitor motivation supported by an increase in destination image because the value of the total influence was at the largest of 0.443.
Fani Fariedah, Maheno Sri Widodo, Soko Nuswantoro
Journal of Aquaculture and Fish Health, Volume 10, pp 101-108; doi:10.20473/jafh.v10i1.22566

Abstract:
Tilapia is euryhaline fish which is commonly cultivated in former shrimp ponds, and belongs to mouthbreeders. The purpose of this study was to determine the hatching success of Tilapia eggs outside the mother's mouth, which was carried out in the salinity medium added with bromelain and papain enzymes. The research method was factorial CRD with enzyme (factor A) and salinity (factor B), with three replications. The research was conducted at the Fish Cultivation laboratory of the Faculty of Fisheries and Marine Sciences, Brawijaya University in January-February 2020. The results showed that papain had more effect on hatchability (93.77%), while the salinity of 5 ppt was the salinity that produced the highest hatchability (93.89%). Moreover, the hatchability was also influenced by the interaction between the enzyme and salinity, namely papain enzyme with a salinity of 5 ppt (98.89%). Meanwhile, bromelain had more effect on the survival of larvae (87.86%), and the salinity that produced the highest survival rate was at 5 ppt (85.75%), but the interaction between enzyme and salinity did not have an effect on the survival of larvae.
M. H. Amiruddin, M. F. Norhalis, Sri Sumarwati, Y. Norma-Rashid
Journal of Aquaculture and Fish Health, Volume 10, pp 25-33; doi:10.20473/jafh.v10i1.18397

Abstract:
Red tilapia (Oreochromis niloticus) is one of the famous fish cultures in the aquaculture industry, especially in Malaysia. The appearance of redness makes the fish more attractive for customers to buy. Product appearance is one of the big criteria that customers choose when they buy food. Bright color effects give high expectations for high quality, nutritious, and healthy food. Therefore, to achieve quality products it is necessary to research how to improve the color quality of tilapia as desired by customers. The purpose of this study was to identify the dietary effect of red paprika used to enhance the coloration of red tilapia. This study is an experimental quantitative study. Red tilapia used as an experimental material was obtained from Freshwater Hatchery, University Malaysia Terengganu. A descriptive analysis was used to analyze the data in this study. This study shows that giving red paprika has an effect on the length and weight of red tilapia, where the highest length and weight gained (1.79 cm and 12.37 g) given red paprika 2% and the lowest length and weight gained (0.63 cm and 2.5 g) which given 4% red paprika. The findings of this study found that only 2% of red paprika treatment showed different growth performance. The crude protein, lipid, and moisture composition of tilapia were also higher at 2% red paprika treatment. Meanwhile, the highest composition of ash is feed with 4% red paprika content. The result of this research showed that the caudal and dorsal either muscle or fin showed the highest color at the fish which fed with 8% red paprika. The finding also found that 8% red paprika containing diet feed for six weeks could improve the coloration of the red tilapia. Future studies suggest the use of Dunaliella salina microalgae which is one of the richest sources of carotenoids used as dyes in food.
Fariq Azhar, Nanang Satria Sumarjan, Siti Hilyana
Journal of Aquaculture and Fish Health, Volume 10, pp 117-126; doi:10.20473/jafh.v10i1.21835

Abstract:
Prevention of disease in tilapia seeds can be done by increasing non-specific defense systems by improving and supplementing the nutritional content of the feed. This study aims to determine the effect of adding Moringa leaf meal combined with probiotics to feed on the survival rate of tilapia seeds and the number of seed blood cells that have been infected with Aeromonas hydrophila. The method used was an experimental method using a Completely Randomized Design (CRD) with 5 treatments and 3 replications. Treatment A = addition of 4% moringa leaf meal + 6 ml / kg of probiotic feed + A. hydrophila injection; Treatment B = addition of 6% moringa leaf meal + 6 ml / kg of probiotic feed + A. hydrophila injection; Treatment C = addition of 8% moringa leaf meal + 6 ml / kg of probiotic feed + A. hydrophila injection; Treatment D = feed + probiotic 6 ml / kg of feed (positive control) + injection of A. hydrophila; Treatment E = feed + probiotic 6 ml / kg of feed (negative control) + injection of physiological solution. The results showed the addition of Moringa leaf meal combined with probiotics got the best results in treatment C with a survival value of 86.67%, hemoglobin levels of 2.3 g%, erythrocytes of 2.20 × 106 cells, leukocytes of 11.5 × 104 cells, and the total number of intestinal bacteria was 10.34 × 106 cfu / ml.
Vini Taru Febriani Prajayati, Otie Dylan Subhakti Hasan, Mugi Mulyono
Journal of Aquaculture and Fish Health, Volume 10, pp 9-16; doi:10.20473/jafh.v10i1.18234

Abstract:
Digestibility is food substances from feed consumption that are not excreted into the feces, and the difference between food substances consumed and those excreted in the feces is the amount of food that can be digested. Information about the digestibility value of food is very important as a basis in assessing the quality of food and designing fish feed rations.This research was conducted to determine the level of the feed digestibility in nirvana tilapia (Oreochromis niloticus) feed with a combination of fish meal and maggot flour. This research was conducted at the Hatchery Campus of BAPPL STP Serang, Banten from September-December 2019. This study used a Completely Randomized Design (CRD) with 5 treatments and 3 replications. There were 225 fish used in the testing with an average weight of 0.16 grams. The culture media used was a plastic container with a size of 60 x 41 x 34 cm. Feeding was carried out for 50 days, with a frequency of twice a day, and the dose of feeding was 3% of the biomass.The results showed the total digestibility parameter of feed, protein digestibility of feed and the highest protein retention in the treatment of 50% of the combination feeds , with the highest total digestibility value of 56.97 ± 3.41 and the lowest of 42.08 ± 0.42, the highest protein digestibility with a value of 87.33 ± 0.95and the lowest of 83.11 ± 1.30 and the highest protein retention with a value of 14.83 ± 0.22and the lowest of 11.69 ± 1.05.
Mochammad Fattah, Susadiana Susadiana, Dwi Sofiati
Journal of Aquaculture and Fish Health, Volume 10, pp 85-94; doi:10.20473/jafh.v10i1.20876

Abstract:
Pangasius catfish is freshwater fish for consumption that can be cultivated through fish farming. In order to produce optimal profits, Pangasius catfish farming must pay attention to the use of inputs in the form of seeds, feed and electricity. The purpose of this study was to analyze the optimization of Pangasius catfish production in Tondo Lestari fish cultivator group, Pagersari Village, Tulungagung Regency. Data collection techniques were in the form of interviews, observation and documentation. The data analysis used was linear programming using the POM-QM for Windows 5 software tool. The Pangasius catfish farming activities of the Tondo Lestari fish cultivator group have been optimal because they resulted in a low production difference between factual and optimal conditons of 3,05 kg or a profit of Rp. 10,502. . The optimal production of Pangasius catfish in a pond size of 200 m2 was 3,173.21 kg and the optimal production in a pond size of 300 m2 was 4,839.83 kg. Therefore, it can be concluded that Pokdakan Tondo Lestari can take advantage of additional feed and electricity input by reducing the use of seed input by 1.487 heads to increase profits.
Catur Pujiono, Akhmad Taufiq Mukti, Woro Hastuti Satyantini
Journal of Aquaculture and Fish Health, Volume 10, pp 1-8; doi:10.20473/jafh.v10i1.17088

Abstract:
Euchema cottoni or Kappaphycus alvarezii is one type of seaweeds that is widely cultivated in Indonesia. Water pollution caused by the accumulation of Pb heavy metals will affect the seaweed to grow and develop. This study is aimed to determine the tannin levels in K. alvarezii and the relationship of Pb heavy metal pollution level with tannin levels in K. alvarezii in different waters. This study used an observational experiment, and samples were taken from two different locations with 4 stations in each. From each sample in both locations, K. alvarezii was taken and was then measured for the tannin levels, water quality, and heavy metals (Pb) in water and in K. alvarezii. The results were analyzed at the Laboratory of PT. Sucofindo and the Laboratory of Testing Service Unit, Airlangga University Surabaya. The data were analyzed using a statistical test in the form of a t-test to determine the differences in samples in both waters, and a C-square test was used to determine the relationship between PB and tannin levels in K. alvarezii. The results showed that the level of Pb heavy metal pollution in the two waters did not show a significant difference due to water quality which was quite similar in both locations. Moreover, the tannin content in K. alvarezii in Bluto waters (0.053±0.004%) was higher than that in Talango waters (0.020±0.009%). Meanwhile, a t-test showed a significant difference in seaweed tannins in both waters. The relationship between Pb heavy metal pollution and tannins of seaweed in Bluto and Talango waters was that they did not affect each other, and tannin concentration was more influenced by chemical oxygen demand (COD), salinity of both waters, and the cultivation methods used.
Abdul Wafi, Heri Ariadi, Abdul Muqsith, Mohammad Mahmudi, Mohammad Fadjar
Journal of Aquaculture and Fish Health, Volume 10, pp 17-24; doi:10.20473/jafh.v10i1.18102

Abstract:
In intensive shrimp culture, oxygen consumption of shrimp is an important indicator that greatly affects the physiological condition of shrimp as a reared organism. The purpose of this study was to dynamically determine the oxygen consumption of shrimp in intensive culture as well as the variables of water quality and shrimp growth. This research was conducted with the concept of ex-post facto causal design in intensive aquaculture ponds during the shrimp cultivation period. During the shrimp culture period, the rate of oxygen consumption of shrimp is inversely related with the sigmodial average increase in shrimp body weight. Meanwhile, based on the simulation analysis with the dynamic modeling concept, it is shown that oxygen consumption was linear to the dynamics of average daily gain and inversely proportional with the increasing rate of shrimp biomass in the ponds. In addition, oxygen consumption rate of shrimp in intensive ponds had a close relationship with water salinity and total organic matter. In conclusion, dynamically, the fluctuation of oxygen consumption rate and average daily gain of shrimp in intensive culture are closely related to the stability of the water quality conditions on the shrimp habitat.
Ockstan Jurike Kalesaran, Cyska Lumenta
Journal of Aquaculture and Fish Health, Volume 10, pp 75-84; doi:10.20473/jafh.v10i1.19567

Abstract:
Chinese pond shell, Sinanodonta (Anodonta) woodiana (Lea, 1834), is a freshwater bivalve that has essential ecological and economic functions. The microstructure of the nacre is of great interest and is the main attraction for the development of pearl farming. This study aims to describe the microstructure and composition of biomineral elements of the nacre at several shell sizes of S. woodiana. The shell is cut with a small forcepon the ventral margin with a size of 3-5 mm for Scanning Electron Microscpy (SEM). SEM images display that a shell layer consists of periostracum, prismatic and nacre layers. The surface of the nacre layer is an irregular or labyrinth patterned. The nacre tablets are hexagonal, glued to each other, so the nacre tablets become polygonal. Moreover, the microstructure of the nacre tablets is like a brick wall, and the thickness of tablets from 0.43 μm to 0.59 μm. The composition of the biomineral elements are C, O, Ca, and the mineralization mechanism is under the control of aquatic environmental factors that help the process of microstructure formation in nacre.
Oktantia Frenny Anggani, Aniek Setiya Budiatin, Laksmi Sulmartiwi, Muhammad Rahmad Royan
Journal of Aquaculture and Fish Health, Volume 10, pp 109-116; doi:10.20473/jafh.v10i1.23424

Abstract:
Scaffold, as one of the components for bone tissue engineering, requires formulated biomaterials that are both structurally and compositively similar to bone composition. Among others, chitosan, gelatin and chondroitin sulfate are known as potential candidates for scaffold composites that can be easily obtained from waste-based resources. This study aims to investigate the cytotoxicity of different scaffold composition and concentration regimes derived from waste-based chitosan, gelatine and chondroitin sulfate, in vitro. The composition regimes used were (Chitosan : Gelatin : Chondroitin Sulfate) 50 : 50 : 0 (A); 50 : 40 :10 (B); 50 : 35 : 15 (C); 50 : 30 : 20 (D); 50 : 25 : 25 (E). Meanwhile, the final concentrations of scaffold used were 2000, 1000, 500, 250, 100, 10 and 0,1 mg/ml. The different compositions and concentrations of scaffold was tested against Hepatocellular Carcinoma (Huh7it / Human Hepatocyte It). After 48-hour incubation in the scaffold solution, the percentage of cell viability was evaluated using 3-(4,5-dimethylthiazol-2yl)-5(3-carboxymethoxyphenyl)-2- (4-sulfophenyl)-2H-tetrazolium (MTT) assay. The result shows that there is no difference observed among different scaffold compositions on the cell viability (p > 0.05). However, different concentrations of scaffold show significant differences in cell viability in composition C and E (p < 0.01), suggesting possible dose- dependent effect of scaffold on cell viability. Overall, all the waste-based scaffold compositions show no toxicity against the Hepatocellular Carcinoma cells as exhibited by the cell viability that is above 70%, at least with the concentration up to 2000 mg/ml.
Indriyani Nur, Hartina Iyen, Yusnaini Yusnaini
Journal of Aquaculture and Fish Health, Volume 10, pp 34-45; doi:10.20473/jafh.v10i1.18926

Abstract:
Eyestalk ablation inflicted upon crustacean is generally performed in captivity to induce ovarian maturation. However, ablation is a hurtful action that may impose physiological stress and even affects survival. The immunologic repercussions of the ablation have not been well understood and thus this study aims to analyze the effect of ablation on immunologic variables through comparing the ablated Litopenaeus vannamei with those of unablated shrimps (control). Unilateral left eyestalk ablation was inflicted on shrimp samples. Shrimp immune responses were observed based on the Total Haemocyte Count (THC), Differential Haemocyte Count (DHC) composing of hyaline cell (HC) counts and granular cell (GC) counts, glucose and uric acid levels, along with the weight gains of shrimps. Observed parametres were taken twice from 10 shrimps for each of the observed and control samples (i.e., on 6 h and 5 d elapsed time). The results show that the ablated shrimps group, after 6 h elapsed time, experienced significant decrease in THC (3.12 ± 0.75 x 106 cells ml-1), but recovered after 5 days (3.89 ± 0.96 x 106 cells ml-1) and were not significantly different from the control (5.16 ± 2.20 x 106 cells ml-1). Meanwhile, glucose level show significant decrease over 6 h and 5 d post ablation (108 and 93 mg dL-1, respectively) compared to the resulting responses from the control (35-36 mg dL-1). Differences in DHC for all cell types as well as uric acid in hemolymph were not significant between the observed and the control samples. Moreover, the ablated treatment did not affect the growth of vannamei after 5 d post ablation. The results revealed that changes occur on the physiological and immune parameters of shrimps due to the eye ablation treatment as shown by the decrease in the THC and glucose levels, especially within the early moments post treatment. However, at the end of the observation (5 d post ablation), shrimps exhibit signs of recovery from stress indicated by an increase in THC close to normal.
Dpn De Silva, Jlcs Perera, Hsd Fernando, Rrmkp Ranatunga, Bgdnk De Silva
Journal of Aquaculture and Fish Health, Volume 10, pp 66-74; doi:10.20473/jafh.v10i1.20905

Abstract:
Swordfish (Xiphias gladius) is a migratory fish commercially exploited due to high export value. Presence of parasites in fish lead to economic losses in the export market and public health issues. This study was conducted to identify the parasite larvae inhabiting swordfish and to determine its phylogenetic origin using ribosomal subunit gene sequence. Parasite samples were extracted from swordfish muscles and five larvae belong to Molicola genus, confirmed by scolex morphology, were used for genomic DNA extraction. Polymerase chain reaction (PCR) was performed to amplify 18S and 28S ribosomal RNA (rRNA) subunit genes followed by Sanger sequencing. DNA sequences were edited by BioEdit software and assembled by CLC genomics version 8.0. Consensus sequences were aligned with NCBI blast to determine the species status. Isolated larval sequences were best aligned with genus Molicola followed by genus Gymnorhyncha. Out of the two published Molicola rRNA gene sequences, 99% identity was observed with Molicola sp. HP5 isolate from Indonesia. Due to lack of sequence data on other Molicola species (except M. thyristes) for comparison, our sequences were published as Molicola sp. Sri Lankan isolates. This is the first record of Molicola sp. in swordfish from Sri Lanka and the results will enhance the knowledge on the distribution of Molicola species while contributing to expand the genetic information on rRNA coding sequences.
Januar Hadi Prasetyo, Agustono Agustono, Widya Paramitha Lokapirnasari
Journal of Aquaculture and Fish Health, Volume 9, pp 232-237; doi:10.20473/jafh.v9i3.18038

Abstract:
Omega-3 fatty acids (Alpha-linolenic acid) and omega-6 fatty acids (Linoleic acid) are a group of essential fatty acids. Essential fatty acids are fatty acids that cannot be synthesized by the body so that must be supplied from the diet. One of the sources of essential fatty acids is derived from fish oil. This study aims to determine the effect of Crude Fish Oil (CFO) in the feed to EPA and DHA content in penaeid shrimp meat. The research method used was a completely randomized design. The treatments used are the varying content of Crude Fish Oil (CFO), which are P0 (0%), P1 (2%), P2 (4%), P3 (6%), and P4 (8%). The results of the study showed significant differences (p
Fenny Amelia, Beni Halalludin, Sidrotun Naim, Rega Permana
Journal of Aquaculture and Fish Health, Volume 9, pp 238-251; doi:10.20473/jafh.v9i3.18855

Abstract:
Udang merupakan salah satu komoditas unggulan nasional dan memiliki nilai ekonomi yang tinggi. Produksi udang budidaya di Indonesia tahun 2012-2017 mengalami kenaikan secara signifikan. Meski mengalami kenaikan, masih terdapat masalah yang harus diatasi, salah satunya adalah penyakit yang disebabkan oleh parasit Enterocytozoon Hepatopenaei (EHP). Infeksi EHP mengganggu sel tubulus hepatopankreas. Lesi yang disebabkan oleh EHP dapat menjadi tempat pertumbuhan koloni Vibrio sehingga meningkatkan kemungkinan munculnya penyakit lain. Penelitian ini bertujuan untuk mengetahui keanekaragaman Vibrio pada udang terinfeksi EHP yang dianalisis dengan Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC PCR). ERIC PCR dapat membedakan strain bakteri yang berkerabat dekat, prosedur kerja yang sederhana, cepat dan murah. Bakteri Vibrio diisolasi dari hepatopankreas, feses dan air udang terinfeksi EHP dan udang sehat (kontrol), dilanjutkan dengan ekstraksi DNA, ERIC PCR, elektroforesis dan konstruksi pohon filogenetik. Hasil penelitian menunjukkan bahwa jumlah bakteri Vibrio pada hepatopankreas, air dan feses udang terinfeksi EHP lebih tinggi dibandingkan dengan udang sehat. Udang terinfeksi EHP memiliki keanekaragaman Vibrio yang lebih rendah dari pada udang sehat. Hal ini menunjukkan adanya spesies Vibrio spesifik yang mendominasi pada udang terinfeksi EHP.
Damian Luis Castellini, Aldo Nahuel Zanazzi, Angelina Gorosito, Enzo Damián Tranier, María Fernández-Subiela, Juan Carlos Mallo
Journal of Aquaculture and Fish Health, Volume 9, pp 266-273; doi:10.20473/jafh.v9i3.19979

Abstract:
In the present work, the feasibility of fattening Oreochromis niloticus specimens on a pilot scale in a geothermal water resource located in the Southeast of the province of Buenos Aires was analyzed. Before placing the culture units, physical and chemical characteristics of hot spring were studied to evaluate its suitability for tilapia farming. In the experimental stage, two hundred specimens with an average weight of 14.5 ± 1.8g were placed in two floating cages (1.30x1x1m) (100 individuals per cage) and they were harvested at 140 days. The specimens were fed 3 times a day with a commercial pelleted feed with a content of 35% of proteins. Monthly samplings were carried out to evaluate growth (weight and total length), survival, and water samples were collected to measure their physical-chemical characteristics. The results of the water analysis showed a high quality in the hot springs (Simplified Water Quality Index=88). The fattening results showed an average final weight of 423-435.9 g, a survival of 87-91%, a SGR 1.47- 1.49% / day and the FCR was estimated at 1.05-1.07 for each cage respectively. We can conclude that this hot spring has the necessary water conditions to obtain excellent weight gains during the fattening period for the O. niloticus specimens. The present study represents the first Nile tilapia culture in the southernmost thermal waters, setting a precedent for future ventures in the area.
Mohamad Amin, Ferdinand Hukama Taqwa, Yulisman Yulisman, Retno Cahya Mukti, Madyasta Anggana Rarassari, Rizky Marli Antika
Journal of Aquaculture and Fish Health, Volume 9, pp 222-231; doi:10.20473/jafh.v9i3.17969

Abstract:
Desa Sakatiga terletak di Kecamatan Indralaya Kabupaten Ogan Ilir, Sumatera Selatan, memiliki potensi pengembangan perikanan budidaya. Permasalahan yang sering dialami oleh pembudidaya ikan lele di desa Sakatiga diantaranya tingginya harga pakan dan minimnya pengetahuan masyarakat tentang bahan lokal yang dapat digunakan sebagai bahan baku pakan ikan. Permasalahan tersebut dapat diatasi dengan membuat pakan secara mandiri. Tujuan dari kegiatan ini adalah mengetahui efektivitas pemanfaatan bahan baku lokal sebagai pakan ikan terhadap peningkatan produktivitas budidaya ikan lele (Clarias sp.) di desa Sakatiga, Kecamatan Indralaya, Kabupaten Ogan Ilir, Sumatera Selatan. Metode yang digunakan yaitu dengan penyuluhan dan diskusi, pelatihan pembuatan pakan serta demplot pemanfaatan bahan lokal sebagai bahan baku pakan ikan serta pendampingan teknis oleh tim dan dibantu mahasiswa yang melaksanakan praktik lapang di lokasi mitra. Berdasarkan hasil yang diperoleh menunjukkan adanya peningkatan pengetahuan masyarakat tentang bahan lokal yang dapat digunakan sebagai pakan ikan serta motivasi masyarakat untuk membuat pakan dengan menggunakan bahan baku lokal. Pemanfaatan bahan baku lokal sebagai pakan ikan lele menghasilkan pertumbuhan bobot dan panjang mutlak masing-masing sebesar 9,94 g dan 3,83 cm, laju pertumbuhan harian 2,21%/hari, efisiensi pakan 103,24% dan kelangsungan hidup 97%.
Fani Fariedah, Maheno Sri Widodo
Journal of Aquaculture and Fish Health, Volume 9, pp 182-188; doi:10.20473/jafh.v9i3.16015

Abstract:
Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi larutan ekstender kurma dan ringer laktat terhadap kualitas spermatozoa beberapa ikan air tawar dalam proses pengawetan spermatozoa. Proses pengawetan sperma tergantung pada nutrisi yang disediakan oleh bahan ekstender. Ringer laktat telah digunakan sebagai ekstender dalam proses pengawetan sperma yang berguna sebagai penyedia energi. Energi lain dapat diperoleh dari bahan yang mengandung fruktosa atau glukosa. Sari kurma merupakan salah satu bahan yang memiliki kandungan fruktosa dan glukosa yang diharapkan dapat membantu memberikan energi bagi sperma selama proses pengawetan sperma. Penelitian ini melakukan percobaan pengawetan sperma dari beberapa ikan air tawar ikan mas (Carrasius auratus), ikan lele siam (Pangasius hypopthalmus), dan Koi (Cyprinus carpio) menggunakan extender yang dibuat dalam kombinasi dari ringer laktat dan larutan sari kurma. Penelitian ini menggunakan berbagai konsentrasi ekstrak kurma yaitu 0,5%, 1%, 2,5%, 2% menggunakan desain rancangan acak kelompok dengan ikan sebagai kelompok. Hasil penelitian menunjukkan bahwa rata-rata motilitas ikan patin mencapai nilai tertinggi (52,00%) sedangkan rata-rata laju fertilisasi tertinggi terjadi pada spermatozoa ikan mas dengan nilai 97,068%. Larutan sari kurma mampu memberikan nutrisi pada spermatozoa selama proses pengawetan karena kurma mengandung fruktosa dan glukosa sehingga spermatozoa yang diawetkan tetap dapat membuahi secara normal.
Akhmad Taufiq Mukti, Septuresty Hartri Eka, Woro Hastuti Satyantini, Ahmad Shofy Mubarak
Journal of Aquaculture and Fish Health, Volume 9, pp 214-221; doi:10.20473/jafh.v9i3.16562

Abstract:
Tujuan penelitian ini adalah mengetahui pengaruh krioprotektan yang berbeda dalam kriopreservasi embrio terhadap persentase kerusakan dan penetasan embrio ikan lele dumbo (Clarias gariepinus). Embrio ikan lele pada fase gastrula diberi perlakuan larutan dimethyl sulfoxide (DMSO), propylene glycol (PG), madu, dan kombinasinya dengan konsentrasi 5% untuk masing-masing perlakuan. Embrio disimpan pada suhu 4 dan 0ºC, masing-masing selama 30 menit, 1, 2, 3, 4, 5, dan 6 jam. Thawing embrio dilakukan pada suhu 28ºC. Selanjutnya, embrio diinkubasi dalam akuarium pada suhu 28ºC. Hasil penelitian menunjukkan bahwa perbedaan krioprotektan dalam kriopreservasi embrio fase gastrula memberikan perbedaan kerusakan dan penetasan embrio ikan lele pada lama waktu penyimpanan yang berbeda. Persentase kerusakan embrio meningkat seiring dengan lama waktu penyimpanan dan suhu penyimpanan yang berbeda. Kombinasi larutan krioprotektan DMSO dan madu serta PG dan madu menunjukkan persentase kerusakan embrio yang lebih rendah dan persentase penetasan embrio yang lebih tinggi dibandingkan perlakuan krioprotektan lainnya (p
Nurlia Subryana, Wardiyanto Wardiyanto, Oktora Susanti
Journal of Aquaculture and Fish Health, Volume 9, pp 194-203; doi:10.20473/jafh.v9i3.16321

Abstract:
Pertahanan non-spesifik adalah pertahanan utama pada benih ikan. Salah satu bahan alami sebagai sumber imunostimulan adalah tanaman kelor. Daun kelor mengandung senyawa flavonoid, alkaloid, terpenoid dan saponin sebagai agen imunostimulan. Imunostimulan adalah senyawa biologis yang dapat meningkatkan sistem kekebalan tubuh.Tujuan dari penelitian ini adalah untuk mengetahui pengaruh ekstrak daun kelor dalam meningkatkan imunitas non-spesifik pada benih ikan nila (Oreochromis niloticus) yang terinfeksi Aeromonas hydrophila. Dalam pemberian ekstrak daun kelor diberikan injeksi intramuskular 0,1 mL/ikan dengan konsentrasi 50 mg, 75 mg dan 100 mg. Pada metode penelitian ini, digunakan Rancangan Acak Lengkap (RAL) dengan 4 perlakuan dan 3 ulangan yang setiap akuarium masing-masing berisi 12 ekor benih nila berukuran 8-10 cm. Pemberian pakan pada benih nila dilakukan setiap 3 kali sehari yaitu pagi, siang dan sore hari. Parameter yang diuji adalah leukosit, diferensial leukosit, eritrosit, aktivitas fagositosis dan indeks. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun pada benih ikan nila dapat meningkatkan kekebalan non-spesifik, yaitu total leukosit, total eritrosit, leukosit diferensial, aktivitas dan indeks fagositosis.
Atiek Pietoyo, Kurniawan Wahyu Hidayat, Siti Nurazizah, Irvan Firmansyah Zainul Arifin, Dh Guntur Prabowo, Fajar Tri Widianto, Irpan Mustakim
Journal of Aquaculture and Fish Health, Volume 9, pp 189-193; doi:10.20473/jafh.v9i3.16191

Abstract:
The ornamental fish business is now an economic driver. The ocellaris clownfish (Amphiprion ocellaris Cuvier, 1830) is one of the leading commodities of marine ornamental fish that is in great demand, especially in foreign markets. One obstacle faced by farmers in determining the appropriate density for the ocellaris clownfish rearing stage. This study aimed to evaluate the effect of stocking density on production factors, which included survival rate, and growth rate of ocellaris clownfish. Three hundred fish were used as experimental fish with an average length of 23.00 ± 2.36 mm and a weight of 0.27 ± 0.02 gather study was conducted using 4 treatment tanks with a volume of 120 L with densities of 0.17, 0.37, 0.67, and 0.80 fish/L. Observations showed that the density of 0.37 fish per L (K2) gave the best results with a survival rate of 98.18%. For the SGR parameter, the highest value was 1.14% per day in the K1 treatment. This indicated that the stocking density has a relationship with the factor of production in rearing ocellaris clownfish in the recirculation system.
Rifqah Pratiwi, Kurniawan Wahyu Hidayat, Sumitro Sumitro
Journal of Aquaculture and Fish Health, Volume 9, pp 274-285; doi:10.20473/jafh.v9i3.16280

Abstract:
Biofloc technology (BFT) is one of the most developed aquaculture technologies, which aims to improve the efficiency of feed use by providing nutrients for flocs to be used by fish as a supplementary feed. Also, BFT serves to improve water quality through the breaking down of fish waste materials assisted by heterotrophic bacteria. Bacteria used in this study were Bacillus sp. as probiotics in BFT. This study aimed to examine the production performance of catfish maintained with a biofloc system on an industrial scale, without experimental design. The average weight of catfish when stocking was 5.9 ± 0.0 g/fish with a density of 7000 fish in 9 unit circular ponds. This studies showed after 78 days of culture, bodyweight gain about 28.6 g/fish to 41.7 g/fish, highest specific growth rate was K2 (2.4 ± 0.2% BW/day), the highest survival rate K2 (98.87 ± 6.64% BW/day), highest grow rate K1 (41.7 ± 5.8 g) and the best FCR K9 (0.95 ± 0.11).
Nwet Darli Kyaw Zaw, Putu Angga Wiradana, Sin War Naw, Aondohemba Samuel Nege, Mochammad Amin Alamsjah, Rizhar Eman Karunia Akbar, Fahror Rosi
Journal of Aquaculture and Fish Health, Volume 9, pp 252-265; doi:10.20473/jafh.v9i3.19255

Abstract:
Caulerpa is one of the seaweed that grows naturally in Indonesian waters such as those in Mandangin Island. This study aimed to identify Caulerpa sp. based on molecular analysis using certain genetic markers. This research is expected to provide information on the identification of macroalgae from Indonesia waters, especially Mandangin Island, Madura with the use of molecular analysis based on 18SrRNA primers. The two green seaweed samples from the Caulerpa genus in this study were successfully analyzed using 18SrRNA primers. The BLAST results of samples 1and 2 are related to Caulerpa taxifolia 18SrRNA, but in the phylogenetic tree result, Sample 1 was more closely related to Caulerpa sertularioides f. longipes. 18SrRNA primers have been used for molecular identification of green seaweed from Mandangin for the first time and this shows that barcode markers can be used for molecular identification of seaweed, specifically Caulerpa in the waters of Mandangin Island, Indonesia.
Hadiana Hadiana, Aweng Eh Rak, Lee Seong Wei, M. Zharif
Journal of Aquaculture and Fish Health, Volume 9, pp 204-213; doi:10.20473/jafh.v9i3.16497

Abstract:
Nowadays, the inclining of Asian clam market demand for supplying the seller Etak in some areas in Malaysia particularly Kelantan where etak had become the main concern due to the stock availability in the natural population was over-exploited. Thus, an effort to expedite the availability seed of Etak must be initiated to conserve the Asian clam population in their habitat. Until now, the information about production larvae is needed to begin the domestication process. The scientific goal aims to evaluate the best time to produce Asian clam larvae to supply the hatchery production process. To answer this question, the evaluation of broodstock conditioning adult Corbicula fluminea monthly during a year and this condition was compared into the fine sand sediment as well as no sediment. The results showed that the Asian clam adult pattern released larvae monthly during a year wherein the great number larvae were produced 22464 Ind/L totally in December and the produced larvae in Oct and Nov was almost similar with range 22350-22500 Ind/L. The condition index with fine sand sediment was 3.8419 % higher than the control treatment 3.5750 %. And last, the ingestion rate both treatment was 1.126 ± 0.534 μg/h and control treatment 1.609± 0.434 μg/h.
Yeni Mulyani, Kiki Haetami, Lesta Krismawati Baeha, Sulastri Arsad,
Journal of Aquaculture and Fish Health, Volume 9, pp 131-142; doi:10.20473/jafh.v9i2.16211

Abstract:
This study aims to determine the effectiveness of Rhizophora Mucronata’s bark extract in curing Vibrio harveyi infection in nile tilapia fish by challenge testing (in vivo test). It was conducted in October 2018-May 2019 at the Central Laboratory of Universitas Padjadjaran and Building-4 of Aquaculture and Hatchery Laboratory in the Faculty of Fisheries and Marine Sciences, Universitas Padjadjaran. This research used microdilution method to determine the Minimum Inhibitory Concentration (MIC) value of R.mucronata’s bark extracts and laboratory experimental method with a Completely Randomized Design (CRD) model for challenge testing (in vivo) which consisted of 5 treatments and 3 replicates where the given treatment varied in concentrations. These various concentrations that were given started from A (0 ppm / control), B (16 ppm), C (32 ppm), D (48 ppm), and E (64 ppm). The observed parameters were inhibitory antibacterial activity for MIC and survival rate, as well as clinical symptoms and water quality for the challenge test. Survival Rate data of nile tilapia seedlings was analyzed by regression analysis. The observation results of MIC, MBC values, clinical symptoms and water quality were analyzed descriptively. The results showed the Minimum Inhibitory Concentration value of R.mucronata bark extract at a concentration of 6,250 ppm while its Minimum Bacteriocidal Concentration value at 50,000 ppm. The fish tolerance test results towards the extract (LC50 test) is 64 ppm. Differences in treatment produced results that have significant effects on survival rate. The use of Rhizophora Mucronata bark extract at a dose of 64 ppm resulted in the highest survival rate of Nile tilapia fish as high as 76.66%.
, Budianto Budianto, Sri Andayani, Dwi Candra Pratiwi
Journal of Aquaculture and Fish Health, Volume 9, pp 164-172; doi:10.20473/jafh.v9i2.16192

Abstract:
Infeksi penyakit menjadi satu masalah yang masih sering timbul pada budidaya ikan patin. Salah satu penyakit yang sering timbul dalam budidaya ikan patin adalah penyakit MES (Motile Edwardsiella Septicemia) atau yang biasa dikenal dengan Edwardsielliosis. Penyebab penyakit MES adalah adanya infeksi dari bakteri Edwardsiella tarda.Bakteri E. tardamerupakan bakteri patogen yang memiliki dampak negatif bagi budidaya ikan air tawar. Analisis darah dilakukan untuk mengetahui kondisi kesehatan ikan yang terinfeksi bakteri E. tarda. Sampel ikan patin diambil dari pembudidaya ikan di Kabupaten Sidoarjo. Hasil identifikasi isolat bakteri dari ikan patin menggunakan uji biokimia yaitu ikan positif terinfeksi Bakteri Edwardsiella tarda. Kondisi kesehatan ikan dapat dilihat melalui pemeriksaan darah meliputi eritrosit, leukosit, differensial leukosit (limfosit, monosit, dan neutrofil) dengan hasil 2,480±0.032; 3,460±0.087; 80,38±0.34; 12,85±0.12; dan 6,77±0.28 secara berturut-turut pada ikan patin normal, sedangkan pada ikan patin yang terinfeksi bakteri E. tardayaitu 1,695±0,041; 7,795±0,095; 52,15±0.26;33,61±0.52; dan 14,24±0.47 secara berturut-turut. Ikan patin yang terinfeksi bakteri Edwardsiella tardamenunjukkan kondisi kesehatan yang kurang baik, yang ditunjukkan dari peningkatan nilai leukosit, monosit, dan neutrofil sebagai bentuk respon kekebalan tubuh terhadap infeksi bakteri.
Moh. Dwi Pratomo, Dian W. Wardanidan, Nur A. Revonagara, Dinda Ersyah, Dwi Setijawati, Hefti Salis Yufidasari,
Journal of Aquaculture and Fish Health, Volume 9, pp 104-113; doi:10.20473/jafh.v9i2.16126

Abstract:
Pepton merupakan sumber nitrogen sebagai media pertumbuhan mikroorganisme. Umumnya, pepton berasal dari hewan darat yaitu sapi dan babi serta turunannya. Penggunaan pepton yang berasal dari babi jelas diharamkan, sedangkan dari sapi dikhawatirkan terdapat penyakit BSE (bovine spongiform encephalopathy), dan TSE (transmissible spongiform encephalopathy) yang dapat menular ke manusia. Sebagai alternatif, pepton dari limbah pengolahan ikan yang sudah terjamin halal, salah satunya limbah ikan kurisi (Nemipterus sp.). Tujuan penelitian ini mendapatkan pepton dengan tiga perlakuan asam berbeda (asam sitrat, asam format dan asam propionat) menggunakan metode eksperimen, dengan rancangan acak lengkap (RAL). Hasil penelitian menunjukkan bahwa perlakuan tiga jenis asam berpengaruh nyata terhadap nilai rendemen, total N, protein, protein terlarut, pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus serta biomassanya. Rata-rata tertinggi rendemen basah didapatkan 66,17% sedangkan rendemen kering 3,87% pada perlakuan asam format. Nilai pH tertinggi 5,3 pada asam sitrat. Uji karakteristik pepton limbah ikan kurisi menunjukan bahwa kandungan protein terlarut berkisar 2-4 g/L, total N 1-1.3 %, dan total protein 6-8.12 %. Asam amino yang terkandung terdiri dari asam amino esensial dan non-esensial. Nilai optical density dan biomassa bakteri ditumbuhkan pada media yang menggunakan pepton limbah ikan kurisi lebih tinggi dibandingkan pertumbuhan bakteri E. coli dan S. aureus pada media yang menggunakan pepton komersial. Temuan ini mengungkapkan bahwa pepton dari limbah ikan kurisi fisibel dikembangkan sebagai produk halal secara komersial untuk media pertumbuhan mikroba.
, Arfan Afandi, Kurniawan Wahyu Hidayat, Rifqah Pratiwi
Journal of Aquaculture and Fish Health, Volume 9, pp 114-121; doi:10.20473/jafh.v9i2.16692

Abstract:
Teknologi bioflok dapat memperbaiki kualitas air pada sistem budidaya ikan lele intensif dan bioflok juga dapat dimanfaatkan sebagai pakan tambahan bagi ikan. Namun adanya kebutuhan oksigen bakteri heterotrof yang sangat banyak pada teknologi bioflok mengakibatkan kandungan oksigen terlarut pada media pemeliharaan relatif rendah sehingga dapat menghambat pertumbuhan ikan. Penelitian ini bertujuan untuk mengevaluasi penggunaan pipa mikro-pori sebagai diffuser aerasi pada budidaya ikan lele intensif berbasis teknologi bioflok. Penelitian ini menggunakan rancangan acak lengkap (RAL) dengan 4 perlakuan dan 3 ulangan. Perlakuan yang diterapkan adalah bentuk atau model difuser pipa mikro-pori berbentuk linear pada sistem bioflok (BFT L), diffuser bentuk sirkular (BFT S), diffuser paralel (BFT P), serta batu aerasi sebagai kontrol (BFT K). Hasil penelitian menunjukkan pipa mikro-pori menghasilkan oksigen terlarut yang lebih tinggi pada kisaran 3-5,7 mg L-1 dibanding kontrol (batu aerasi). Tingkat kelangsungan hidup ikan tertinggi terdapat pada perlakuan BFT L sebesar 78,67% P
Umidayati Umidayati, Sinung Rahardjo, Ilham Ilham,
Journal of Aquaculture and Fish Health, Volume 9, pp 122-130; doi:10.20473/jafh.v9i2.16139

Abstract:
Cacing sutra merupakan pakan alami yang sangat dibutuhkan pada pembenihan ikan air tawar namun ketersediaannya tidak kontinyu dikarenakan kondisi alam yang terbawa arus pada musim penghujan, keberadaan cacing sutra dialam juga di pengaruhi oleh banyaknya bahan organik yang melimbah dari limbah rumah tangga yang cendung adanya kontaminasi bakteri yang terdapat pada cacing sutra tangkapan dari alam.Untuk memenuhi kebutuhan kontinyunitas cacing sutra di budidayakan dengan media yang sering adalah kotoran ayam . Untuk penyedian pakan alami larva ikan sebaiknya di identifikasi bakteri pada cacing tangkapan dari alam dan hasil budidaya. Penelitian dilakukan di Laboratorium Basah Sekolah Tinggi Perikanan Jakarta pada bulan Mei – Juli 2019 dengan tujuan mengidentifikasi bakteri salmonella sp. pada media kotoran ayam dan cacing hasil tangkapan alam dan hasil budidaya. Metode Identifikasi digunakan untuk penelitian ini dan hasilnya dianalis secara deskriptif. Hasil penelitian menunjukkan pada cacing sutra dari alam positif mengandung salmonella sp dan sedangkan untuk hasil budidaya negatif tidak mengandung Salmonella sp.
, Mirni Lamid, Koesnoto Soepranianondo, M. Anam Al-Arif, Moch. Amin Alamsjah, Soeharsono Soeharsono
Journal of Aquaculture and Fish Health, Volume 9, pp 95-103; doi:10.20473/jafh.v9i2.15783

Abstract:
Asian white shrimp or can be called vannamei shrimp has the Latin name Litopenaeus vannamei has native habitat in coastal waters and American sea waters. This research was carried out in September 2018 in the Asian white shrimp farming business unit Tanjung Putih Village Sepulu District Bangkalan District and in Penatar Sewu Village Tanggulangin District Sidoarjo Regency. This study population used 3 pond plots in Asian white shrimp culture in Tanjung Putih Village, Sepulu District, Bangkalan District and in Penatar Sewu Village Tanggulangin District Sidoarjo Regency. Sample size is determined from the amount of shrimp fry capacity stocked. The sampling technique was carried out by researchers by following activities in the field, census and visiting respondents directly on the farm or in the farmer's group home to obtain the information and data needed. The results showed that the best feed management was obtained the best results on the 4 sample ponds in Sidoarjo with an average final weight growth rate of 9.73 grams at the first partial harvest, 83% Feed Efficiency, and a 90% survival rate. As for the lowest feed management results obtained in sample ponds 1 Bangkalan with a yield of 6.98% growth, feed efficiency by 71% and survival rate of 86%. As for the results of the analysis of vannamei shrimp aquaculture business at different locations in Bangkalan and Sidoarjo districts, it was concluded that the best Production BEP was obtained in Sidoarjo's 4 sample ponds, amounting to 1427 kg. The best R / C ratio and Payback period is the average sample obtained in Sidoarjo. The conclusion of the best feed management and business analysis results is on the sidoajo sample ponds, the sidoarjo sample shows decent and efficient results.
Syamsu Alam, Andi Adam Malik, Khairuddin Khairuddin
Journal of Aquaculture and Fish Health, Volume 9, pp 173-181; doi:10.20473/jafh.v9i2.16814

Abstract:
Penelitian ini bertujuan untuk mengetahui kadar salinitas yang berpengaruh optimal terhadap laju respirasi, pertumbuhan, dan sintasan ikan mas. Benih ikan mas sebagai sampel dengan panjang 3-5 cm yang diproduksi oleh BBI Karrang dengan padat tebar 20 ekor/m2 (SNI 01-6133,1999). Pakan yang digunakan adalah pakan komersial berkadar protein 36% dengan dosis 20% dari berat badan. Salinitas dibuat dengan menambahkan garam curah (tidak beryodium) pada media kultur sesuai dengan perlakuan. Rancangan penelitian yaitu Rancangan Acak Lengkap. Laju respirasi tertinggi pada perlakuan D dan terendah pada A. Pertumbuhan berat pada minggu 1 seragam, selanjutnya minggu 2 mulai bervariasi. Minggu kedua hingga akhir penelitian pengaruh faktor nutrisi menjadi penting dimana penundaan pemberian pakan mempengaruhi pertambahan bobotnya. Laju pertambahan bobot tertinggi didapatkan pada perlakuan B (2.77 gram), selanjutnya A (2.74 gram), C (2.62 gram), dan D (2.58 gram). kosentrasi salinitas media kultur berpengaruh nyata terhadap sintasan benih ikan mas. Sintasan benih ikan mas semakin menurun dengan bertambanya waktu penelitian. Pemeliharaan benih ikan mas pada salinitas yang berbeda berpengaruh terhadap sintasan dan laju respirasi, tetapi tidak berpengaruh terhadap pertumbuhannya semakin tinggi salinitas maka pertumbuhan, sintasan semakin menurun, namun laju respirasi cenderung meningkat. Konsentrasi salinitas media kultur yang optimal untuk pemeliharaan benih ikan mas yaitu 4 ppt.
, M. Fauzan Isma, Rindhira Humairani, Yusrizal Akmal
Journal of Aquaculture and Fish Health, Volume 9, pp 86-94; doi:10.20473/jafh.v9i2.15810

Abstract:
This study was conducted to investigate the digestive system development and early feeding activity of Pangasius hybrid larvae. Four crossbreeding program in triplicate were examined between Pangasius hypopthalmus and Pangasius nasutus. Results showed that the digestive systems of both hybrid larvae were poorly developed immediately after hatching. The digestive system of the newly hatched larvae consisted of straight undifferentiated tube and anus still closed. The gastro intestinal tract, liver and pancreas were formed and developed by 2-dAH. The zymogen granules were also observed in the pancreas and thus imply that function of pancreas started before the exogenous feeding period. Gastric glands were only present and started to secrete enzyme on 6-dAH. Artemia were first seen in digestive tract of the larvae at 54-hAH. However, onset of exogenous feeding was detected at 60-hAH, whereas stomach became enlarged with isometric epithelium cells, which were different from the elongated cells in the intestinal part. Keywords: Pangasius, hybrid, digestive system, histology
Lutfiyah Al Adawiyah, , Hapsari Kenconojati
Journal of Aquaculture and Fish Health, Volume 9, pp 155-163; doi:10.20473/jafh.v9i2.17183

Abstract:
Utilization of Porphyridium sp. began to be developed as an anticancer, anti-inflammatory, antibacterial, antifungal, and production of biogas and biodiesel so that necessary to carry out culture activities for ensure continuity of Porphyridium sp. This study aims to determine the type of culture container that showed the best growth response of Porphyridium sp. with the highest biomass. The stages in this research were preparation of containers and media sterilization, making diatom fertilizers and agar media, Culture of Porphyridium sp. on agar media, Culture of Porphyridium sp. in the test tube, and Culture of Porphyridium sp. in glass and plastic containers. The results showed that the growth response of Porphyridium sp. that cultured in glass containers (18,9 ± 0,21 x 105 cells/mL) was higher than plastic (15.57 ± 0,03 x 105 cells/ml).
Wiyoto Wiyoto, Irzal Effendi
Journal of Aquaculture and Fish Health, Volume 9, pp 143-154; doi:10.20473/jafh.v9i2.17192

Abstract:
Finding a good location is of important aspects in mariculture. This can be done by evaluating the water quality data. The aims of the study were to evaluate the seawater quality at Moro, Karimun, Riau Islands and to analyze its compatibility for mariculture by using principal component analysis (PCA) and multiple linear regressions. Generally, seawater qualities in the study area were in the tolerance range for mariculture. Surface water samples were collected from five different sampling points around Moro Sea. PCA results demonstrated that there were eleven variation factors which explained 95.4% of the total variance. In addition, based on PCA and multiple linear regressions, four water quality predictors for environmental quality could be identified, that is nitrite (NO2), temperature, pH and dissolved oxygen. Multiple linear regressions showed that the contribution of each parameter to the water quality was significant (R2=1, P < 0.05).
Reni Oktaviani, , Sudarno Sudarno
Journal of Aquaculture and Fish Health, Volume 9, pp 20-29; doi:10.20473/jafh.v9i1.15443

Abstract:
The production M. macrocopa ephippia limited by availability of poly unsaturated fatty acids (PUFAs) in feed. The lack amount of ω-3 fatty acids in rice bran suspension limits ephippia production. Lemuru fish oil containing EPA has potential to enrich in rice bran suspension to increase ephippia production. The purpose of this study to determine the affect addition of lemuru fish oil in rice bran suspension and the optimal concentration of lemuru fish oil in rice bran suspension which results the highest ephippia production. This study used Completely Randomized Design (CRD) method with 5 treatments adding fish oil in rice bran suspension of 0%, 1.5%, 3%, 4.5%, and 6%), with 4 replications. Sexual female offspring are produced from cultures with density 660 ind /L with regulated feed concentration. Furthermore, offspring are cultured with feed according the treatment. During the culture, daily production, total production and production ephippia per parent. These results indicate addition of lemuru fish oil to rice bran suspension as feed of M. macrocopa affect the production ephippia. The addition of lemuru fish oil with concentration 6ml / 100g in rice bran produce the highest total M. macrocopa ephippia production (3452 ± 43.0 grains / Liter)
Dara Puspita Anggraeni, Muhamad Ali, Ali Haris, Muhamad Amin
Journal of Aquaculture and Fish Health, Volume 9, pp 1-10; doi:10.20473/jafh.v9i1.15367

Abstract:
Fitat merupakan salah satu senyawa antinutrisi pada pakan dengan bahan dasar yang mengandung biji-bijian seperti kedelai. Senyawa tersebut dapat mengikat protein dan mineral-mineral penting di dalam saluran pecernaan ikan sehingga dapat menyebabkan daya cerna pakan menjadi rendah. Senyawa yang dapat memecah fitat adalah enzim fitase. Oleh karena itu, penelitian ini bertujuan untuk mengetahui pengaruh penambahan bakteri penghasil enzim fitase (Lactobacillus plantrarum) terhadap tingkat pertumbuhan dan kelangsungan hidup ikan nila. L. plantarum diberikan melalui penyemprotan pada pakan komersial dengan konsentrasi berbeda; 0 log CFU/g sebagai kontrol (P1); 3.50 ± 0.24 log CFU/g (P2), dan 7.05 ± 0.11 log CFU/g (P3). Ikan nila dipelihara pada bak plastik selama 45 hari dan diberi pakan komersil dengan dan campuran probiotik tersebut diatas. Hasil penelitian menunjukkan bahwa konsentrasi probiotik berpengaruh secara signifikan terhadap tingkat pertumbuhan dan kelangsungan hidup ikan nila. Pertumbuhan terbaik diperoleh pada konsentrasi 7.05 ± 0.11 log CFU/g (P3) dengan laju pertumbuhan spesifik 3.04 % BW/hari (berat) dan 2.89% BL/hari (panjang). Disamping itu, ikan yang menerima suplementasi probiotik juga menunjukkan nilai rasio konvesrsi pakan yang lebih baik, yakni 1.25±0.03, dibandingkan dengan kontrol (1.33 ±0.03). Namun tingkat kelangsungan hidup ikan nila yang diberi tambahan probiotik tidak berbeda nyata dengan kontrol (tanpa penambahan probiotik), ~91%. Hasil ini menunjukkan bahwa Oleh karenanya, probiotik Lactobacillus plantrarum ini sangat direkomendasikan untuk dilakukan ujicoba pada skala yang lebih besar.
Seto Sugianto Prabowo Rahardjo, Nailul Izzah
Journal of Aquaculture and Fish Health, Volume 9, pp 81-85; doi:10.20473/jafh.v9i1.11818

Abstract:
ABSTRAKIkan komet (Carassius auratus) merupakan salah satu komoditas ikan hias yang digemari masyarakat karena keindahan dan pergerakan pada tubuhnya yang membuat masyarakat Indonesia tertarik terhadap ikan ini. Dengan semakin banyaknya masyarakat yang tertarik terhadap ikan ini maka diperlukan pembenihan ikam komet yang benar. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian pakan alami dan pakan buatan terhadap hatching rate (HR) dan survival rate (SR) serta morfologi ikan komet. Metode penelitian ini secara deskriptif dengan menggunakan 2 perlakuan pemberian pakan alami yaitu chlorella sp dengan daphnia sp dan pellet PF800. Hasil penelitian ini menunjukkan bahwa pemberian pakan alami (chlorella sp+daphnia sp) dan pakan pellet PF800 berpengaruh terhadap hatching rate (HR), survival rate (SR) dan morfologi ikan komet. Perlakuan pemberian pakan pellet PF800 menunjukkan hasil terbaik yaitu hatching rate (HR) sebesar 93,98% ; survival rate (SR) sebesar 85,12%. Hal ini dikarenakan jumlah nilai gizi pada pakan alami dan pellet PF800 sangat baik untuk pertumbuhan larva ikan komet sedangkan pakan alami memberikan warna tubuh pada ikan komet paling cerah bila dibandingkan dengan pakan pellet PF800. Kata kunci : Ikan komet, pellet, chlorella, daphnia
, Gunanti Mahasri, Koesnoto Koesnoto
Journal of Aquaculture and Fish Health, Volume 9, pp 75-80; doi:10.20473/jafh.v9i1.16215

Abstract:
Penelitian ini bertujuan untuk mengetahui patogenesis infestasi Gyrodactylus yang menginfestasi insang ikan mas (Cyprinus carpio). Patogenesis diamati berdasarkan tingkat derajat infestasi, pengamatan gejala klinis dan patologi insang ikan mas (Cyprinus carpio). Sebanyak 1.440 ekor ikan mas terinfestasi Gyrodactylus diambil dan diamati gejala klinis, penentuan derajat infestasi serta dilakukan pengamatan patologi anatomi dan histopatologi insang. Hasil pengamatan sampel menunjukkan pada tingkat infestasi ringan dan berat, kerusakan yang ditimbulkan antara lain hiperplasia, hipertropi dan hemoragi. Sedangkan ikan mas normal menunjukkan gambaran histopatologi insang yang normal.
, Prive Widya Antika, Arning Wilujeng Ekawati, Nasrullah Bai Arifin
Journal of Aquaculture and Fish Health, Volume 9, pp 38-47; doi:10.20473/jafh.v9i1.15769

Abstract:
Spirulina platensis is a filamentous cyanobacterium that has been commerically used for fish feed and human food supplement. Low-cost production of Spirulina is needed when considering large-scale culture especially for industrial purposes. The aim of this study was to explain the effect of calcium nitrate (Ca(NO3)2) on growth, biomass, pigment, and protein production of S. platensis and to determine the best calcium nitrate concentration for Spirulina production.The microalgae was cultured at four calcium nitrate concentrations (1, 1.5, 2.0 and 2.5 g/L) with salinity of 15 ppt, constant light intensity of 4,000 lux and photoperiod of 24:0 light:dark cycles for 4 days. The results showed that different calcium nitrate concentrations remarkably affected the growth, biomass production, pigment and protein content of S. platensis (p
, Hartati Kartikaningsih, Mimit Primyastanto, Supriyadi Supriyadi, Rhytia Ayu Christianty Putri
Journal of Aquaculture and Fish Health, Volume 9, pp 11-19; doi:10.20473/jafh.v9i1.15292

Abstract:
This time, in the digital era, a business cannot be separated from digital marketing, this is by the explanation of technological developments. The existence of Small and Medium Enterprises (SMEs) in Indonesia is a driving factor in the creation of national economic development because it can spur economic growth. The high use of social media, especially Facebook in Indonesia, encourages business people to use social media as marketing media. This is also done by Anugerah Mina Lestari SMEs. So that researchers feel they are researching the Effectiveness of the UKM Mina Lestari Marketing System Using Facebook Social Media. The research used the EPIC Model which involved 80 samples. The sample is part of the population of product buyers from Anugerah Mina Lestari SMEs. The effectiveness of the Anugerah Mina Lestari SME marketing system through Facebook social media that was analyzed using the EPIC Model with four variables (Empathy, Persuasion, Impact, Communication), showed an EPIC Rate of 2.943 which means that the Anugerah Mina Lestari SMEs marketing system through Facebook social media can be assessed effectively.
, Andik Isdianto
Journal of Aquaculture and Fish Health, Volume 9, pp 57-68; doi:10.20473/jafh.v9i1.15379

Abstract:
Lokasi Batam sangat strategis karena terletak di rute perdagangan internasional dan terletak di pusat segitiga pertumbuhan Indonesia-Malaysia-Singapura (IMS-GT). Mempertimbangkan meningkatnya permintaan lahan di Pulau Batam, Pemerintah Kota Batam telah memperluas area melalui proses reklamasi untuk kawasan industri dan komersial lainnya. Proses reklamasi secara signifikan berdampak pada penurunan kualitas lingkungan di Pulau Batam. Kondisi kerusakan akan semakin parah sehingga akan menyebabkan buruknya kualitas perairan akibat pencemaran perairan pesisir dan laut. Salah satu area reklamasi adalah Tering bay. Reklamasi teluk Tering saat ini dalam kondisi dengan kualitas air yang sangat buruk. Tujuan dari penelitian ini adalah mempelajari pengelolaan kawasan teluk Tering yang berkelanjutan, mengingat reklamasi merupakan kegiatan yang menjadi kebutuhan masyarakat.Metode yang digunakan dalam penelitian ini menggunakan metode Partial Least Square (PLS) dan Analytical Hierarchy Process (AHP). PLS digunakan untuk menentukan ada tidaknya hubungan antara variabel laten dalam reklamasi pantai, serta membentuk model konstruktif. Sementara itu, metode AHP berguna untuk menentukan skala prioritas penanganan pengelolaan kawasan reklamasi pesisir yang berkelanjutan. Hasil penelitian menunjukkan bahwa untuk mencapai reklamasi berkelanjutan diperlukan pengelolaan yang baik. Manajemen meliputi: peningkatan peran masyarakat, pemerintah daerah, sektor swasta dan pengetatan peraturan. Namun, manajemen harus memperhatikan proses erosi dan abrasi dengan mempertimbangkan bahwa wilayah ini memiliki gelombang laut yang cukup kuat. Abstract Batam's location is very strategic as it lies on international trade routes and is located in the center of Indonesia-Malaysia-Singapore (IMS-GT) growth triangle. Considering the increasing land demand in Batam Island, Batam City government has expanded the area through reclamation process for industrial and other commercial area. Reclamation process has significantly impacted the deterioration of environmental quality in Batam Island. The condition of the damage will become more severe so that it will cause the poor quality of waters due to pollution of coastal and marine waters. One of the reclaimed areas is Tering bay. The reclamation of the Tering bay is currently under conditions with very poor water quality. The purpose of this research is to study the management of Tering bay area which is sustainable, considering the reclamation is an activity that become the society need.The method used in this research using Partial Least Square (PLS) and Analytical Hierarchy Process (AHP) method. PLS is used to determine whether or not there is a relationship between latent variables in coastal reclamation, as well as forming a constructive model. Meanwhile, AHP method is useful to determine the priority scale of handling management of coastal reclamation area sustainable.The results show that to achieve sustainable reclamation requires good management. The management includes: increased role of community, local government, private sector and tightening of rules. However, the management should pay attention to the process of erosion and abrasion with considering that this region has a strong enough sea waves.
, Ahmad Taufiq Mukti, M. Yasin
Journal of Aquaculture and Fish Health, Volume 9, pp 69-74; doi:10.20473/jafh.v9i1.15915

Abstract:
Udang merupakan salah satu komoditi ekspor unggulan di Indonesia. Udang juga merupakan salah satu produk perikanan yang mudah busuk (high perishable), oleh karena itu dibutuhkan suatu proses untuk meminimalisir pembusukan. Pengawetan adalah salah satu cara untuk meminimalisir pembusukan. Cara yang sering digunakan untuk mempertahankan kualitas atau mengawetkan suatu produk perikanan adalah dengan menggunakan es batu,. Akan tetapi, banyak pelaku bisnis menilai tidak praktis dan harganya relatif mahal, akhirnya menggunakan formalin untuk proses pengawetan. Penggunaan formalin pada makanan dapat membahayakan manusia jika terpapar lebih dari ambang batasnya. Selain itu, penggunaan formalin sebagai pengawet pada makanan juga tidak diperboehkan, baik dalam peraturan dalam maupun luar negeri. Penelitian ini bertujuan untuk mengetahui kandungan formalin pada udang vaname setelah direndam pada formalin dengan dosis yang berbeda. Deteksi formalin tersebut dilakukan di balai besar laboratorium kesehatan (BBLK), Surabaya menggunakan spektrofotometri UV-Vis. Dari penelitian tersebut didapatkan hasil fungsi linier, semakin tinggi konsentrasi formalin yang diberikan, maka semakin banyak formalin yang terkandung dalam udang vaname dengan mendapatkan hasil tertinggi yaitu 430,2 ppm pada perlakuan perendaman 5 % selama satu jam.
Sudewi Sudewi, Zeny Widiastuti, Ida Komang Wardana, Ketut Mahardika
Journal of Aquaculture and Fish Health, Volume 9, pp 48-56; doi:10.20473/jafh.v9i1.15913

Abstract:
Among the high significance diseases in spiny lobster farming, Milky Hemolymph Disease of Spiny Lobster (MHD-SL) is considered as the most devastating disease which caused in mortality up to 80 % and morbidity to 100%. Investigations of this disease were mostly performed for farmed lobster and no observation was conducted for wild lobster. This study was carried out first, to investigate milky hemolymph disease occurrence both in wild and farmed spiny lobster Panulirus homarus from different locations by PCR analysis. Second, to perform nucleotide sequence analysis in order to identify, and to describe relationship of the milky disease agent obtained in this study with the disease agent from several geographical regions by phylogenetic analysis. Adult farmed lobsters were collected from Lombok, Pangandaran and Pegametan, while wild lobsters were obtained from Jembrana, Lombok, and Banyuwangi coastal areas. One farmed lobster from Pegametan and two farmed lobsters from Lombok were infected with the milky disease. Nucleotide sequence analysis demonstrated that the causative agent of milky disease obtained in this study (MHD-1, 2, and 3) exhibited 99% homologous nucleotide sequence with milky disease agent from Vietnam that was uncultured Rickettsia-like Bacteria (RLB). As a consequence, further work is needed most importantly on how to cultivate milky disease agent in order to find proper methods to alleviate milky disease problems. This paper is the first report on phylogenetic analysis of milky disease from Indonesia that pointed out a closed relationship between milky disease in the present study and from Vietnam, Madagascar, Mozambique and Tanzania.
, Siti Nurazizah, Furqon Saepul Rohman
Journal of Aquaculture and Fish Health, Volume 9, pp 30-37; doi:10.20473/jafh.v9i1.15667

Abstract:
Peningkatan produksi ikan mas (Cyprinus carpio) tidak terlepas dari ketersediaan bibit ikan mas. Maka diperlukan keberlanjutan bibit ikan mas, salah satu nya adalah bibit ikan mas marwana. Keberlanjutan bibit ikan mas marwana dapat dilakukan dengan menerapakan system manajemen dalam kegiatan produksi bibit ikan mas marwana. Penerapan system manajemen pembenihan dapat dimulai dari proses perencanaan, pelaksanaan dan pengawasan. Analisis data menggunakan analisis deskriptif kualitatif yang berasal dari data hasil pengamatan, observasi, wawancara dan partisipasi langsung dan kemudian di deskripsikan dalam bentuk uraian singkat. Kegiatan pembenihan ikan mas marwana harus dilakukan dengan manajemen pembenihan yang tepat dan sesuai untuk menunjang keberhasilan pembenihan. Pembenihan dapat dimulai dengan perencanaan pemeliharaan induk, manajemen pakan induk dan seleksi induk. Selain itu penerapan system pelaksanaan persiapan pemijahan, proses pemijahan, pasca pemijahan, penetasan telur dan pemeliharaan larva. Penerapan system perencanaan dan pelaksanaan dikontrol dengan menerapkan system pengawasan pembenihan yang bertujuan untuk mengambil tindakan pembetulan yang diperlukan melalui upaya pengendalian. Upaya pengendalian dalam system pengawasan terdiri dari pengendalian terhadap hambatan dan gangguan.
Arning Wilujeng Ekawati, Muhammad Fakhri, Joko Abdillah, Wiwit Nor Indahsari
Journal of Aquaculture and Fish Health, Volume 8, pp 149-158; doi:10.20473/jafh.v8i3.15048

Abstract:
Tujuan penelitian ini untuk menentukan jumlah terbaik pemanfaatan tepung limbah bandeng (Chanos chanos) sebagai sumber protein pengganti tepung ikan dalam pakan udang galah (M. rosenbergii de Man). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 5 perlakuan dan 3 ulangan. Pakan yang digunakan sebagai perlakuan adalah pakan iso protein 35% dan iso energi 3,40 kkal/g dengan perbedaan subtitusi protein tepung limbah bandeng terhadap tepung ikan, yaitu: (A) 0%; (B) 25%; (C) 50%; (D) 75%; dan (E) 100%. Parameter yang diamati dan dianalisis terdiri dari: kelulushidupan, laju pertumbuhan spesifik, rasio konversi pakan, rasio efisiensi protein, dan retensi protein pada udang galah (M. rosenbergii de Man). Hasil penelitian menunjukkan bahwa pemanfaatan tepung limbah bandeng sebagai sumber protein hewani pengganti tepung ikan dengan persentase yang berbeda dalam formula pakan tidak berpengaruh nyata terhadap semua parameter yang diamati pada udang galah (M. rosenbergii de Man). Berdasarkan hasil penelitian dapat disimpulkan bahwa pemanfaatan tepung limbah bandeng dapat menggantikan tepung ikan sebagai sumber protein dalam pakan udang galah (M. rosenbergii de Man) hingga 100%.
, Eyo Victor Oscar
Journal of Aquaculture and Fish Health, Volume 8, pp 191-198; doi:10.20473/jafh.v8i3.14789

Abstract:
This study was aimed to investigate the effect of Buchholcia coriacea on the masculinization of Oreochromis niloticus. Five days old mixed sex of O. niloticus (mean weight 0.2g, mean length 0.45mm) were subjected to powdered B. coriacea seeds meal (BSM) at 0, 2, 4, 6, 8, 10g/kg twice daily for 1 month in an indoor experimental plastic basin capacity (50cm in diameter and 30cm deep) in duplicates with a total of six treatments in a complete randomized design. After which there were later fed with a normal diet in an outdoor concrete tank of size 1m3 for another one month. One way ANOVA and LSD were used to analyze the experimental data. The result obtained in this study showed that fish fed with 4g/kg BSM powdered BSM had the highest number (83.33%) of males which was significantly higher (p
Kurniawan Wahyu Hidayat, Ighfirli Amatullah Nabilah, Siti Nurazizah, Bobby Indra Gunawan
Journal of Aquaculture and Fish Health, Volume 8, pp 123-128; doi:10.20473/jafh.v8i3.12931

Abstract:
Udang Vaname (Litopenaeus vannamei) merupakan salah satu jenis udang yang memiliki prospek dan profit yang menjajikan, hal ini ditunjukan oleh harga dan permintaan yang relatif tinggi dari tahun ke tahun. Dalam kegiatan budidaya udang vaname harus memerhatikan segala aspek mulai dari persiapan lahan sampai dengan saat panen. Dengan berkembangnya teknologi, kegiatan budidaya udang vaname dilakukan dengan teknologi dan inovasi baru yaitu budidaya udang vaname supra intensif. Kegiatan budidaya supra intensif adalah mengimplementasikan sistem budidaya yang konsisten dan terkontrol, yaitu dengan menggunakan benih yang bermutu, nutrisi yang cukup, dan dikelola dengan manajemen modern. Tujuan dari praktek kerja lapang ini adalah untuk mengetahui proses kegiatan pembesaran udang vaname di PT. Dewi Laut Aquaculture dari mulai persiapan lahan, sterilisasi, pembentukan air, penebaran benur, pemeliharaan sampai panen. Metode kerja yang digunakan dalam Praktik Kerja Lapang adalah dengan cara mengikuti langsung kegiatan pembesaran udang vaname dimulai dari persiapan lahan sampai dengan panen. Dari hasil pemeliharaan selama 65 hari menghasilkan panen dengan SR 85,05%, biomasa 1080,54 kg dengan ABW 6,41g/ekor. Kualitas air yang diukur selama praktik masih dalam kisaran yang optimal bagi pertumbuhan udang yaitu Suhu air (°C) 28 – 30, pH 7,5 – 8,5, DO 4,78 – 7,30, serta amonia 0,01 – 0,21. Dikarenakan terindikasi Infectious Myonecrosis Virus (IMNV), maka udang dipanen lebih awal.
, Dyah Setyawati, Nurul Maulida
Journal of Aquaculture and Fish Health, Volume 8, pp 159-166; doi:10.20473/jafh.v8i3.15059

Abstract:
Ikan lele (C. gariepinus) merupakan jenis ikan konsumsi yang mulai merebut perhatian pelaku usaha budidaya karena dapat dilakukan pada lahan sempit dan jumlah air yang terbatas dengan padat tebar tinggi. Terbatasnya sumber daya alam seperti air dan lahan, menjadikan intensifikasi sebagai pilihan yang paling memungkinkan dalam meningkatkan produksi budidaya ikan lele. Metode yang digunakan dalam penelitian ini adalah metode deskriptif yaitu suatu peneitian yang diakukan untuk pemberian gambaran secara objektif. Perlakuan yang digunakan adalah padat penebaran yang berbeda (100 ekor/m3, 300 ekor/m3, dan 500 ekor/m3) untuk mengetahui komunitas bakteri pada kepadatan berbeda. Pengambilan sample air dilakukan 3 kali selama masa pemeliharaan yaitu hari ke-1, hari ke-30 dan hari ke-60. Sedangkan parameter pendukung yaitu kualitas air dilakukan tiap pagi dan sore selama 60 hari. Terdapat tiga komponen dalam sistem resirkulasi yang digunakan yaitu kolam pemeliharaan ikan, bak swirl-filter, dan bak biofilter. Pada kolam 1 hari pertama menunjukkan kepadatan 5.7x104 CFU/mL, spesies yang ditemukan yaitu Bacillus subtilis dan Bacillus megaterium. Pada kolam 1 hari ke-30 menunjukkan kepadatan 8.3x104 CFU/mL, spesies yang ditemukan yaitu Bacillus subtilis. Pada kolam 1 hari ke-60 menunjukkan kepadatan 3.1x104 CFU/mL, spesies yang ditemukan yaitu Empedobacter brevis. Pada kolam 2 hari pertama menunjukkan kepadatan 3.2x103 CFU/mL, spesies yang ditemukan yaitu Bacillus subtilis. Pada kolam 2 hari ke-30 menunjukkan kepadatan 3.5x103 CFU/mL, spesies yang ditemukan yaitu Spingomonas paucimobilis. Pada kolam 2 hari ke-60 menunjukkan kepadatan 3.7x105 CFU/mL, spesies yang ditemukan yaitu Empedobacter brevis. Pada kolam 3 hari pertama menunjukkan kepadatan 1.4x103 CFU/mL, spesies yang ditemukan yaitu Staphilococus vitulus dan Bacillus megaterium. Pada kolam 3 hari ke-30 menunjukkan kepadatan 1.7x103 CFU/mL, spesies yang ditemukan yaitu Stapilococus xylosus dan Bacillus cereus. Pada kolam 3 hari ke-60 menunjukkan kepadatan 3.6x105 CFU/mL, spesies yang ditemukan yaitu Bacillus subtilis dan Bacillus lincheniformis.
, Husnul Khotimah, Ade Dwi Sasanti, Sefti Heza Dwinanti, Madyasta Anggana Rarassari
Journal of Aquaculture and Fish Health, Volume 8, pp 139-148; doi:10.20473/jafh.v8i3.14901

Abstract:
Akuaponik adalah gabungan budidaya ikan dan tanaman hidroponik dengan sistem resirkulasi. Akuaponik merupakan salah satu alternatif yang dapat memanfaatkan limbah budidaya ikan serta mampu menghemat penggunaan lahan dan air. Tujuan dari kegiatan ini adalah memberikan informasi kepada para pembudidaya mengenai pemeliharaan ikan nila dan tanaman kangkung dengan sistem akuaponik. Kegiatan ini dilaksanakan pada bulan September sampai November 2018. Pemeliharaan ikan nila dengan sistem akuaponik dilakukan selama 37 hari, dikolam beton ukuran 3x1,5x1 m3, dengan jumlah ikan yang ditebar 200 ekor. Hasil pemeliharaaan didapatkan pertumbuhan ikan nila sebesar 3,31±0,68 cm dan 21,59±4,28 g. Laju pertumbuhan spesifik ikan nila sebesar 2,07%.hari-1 dengan kelangsungan hidup sebesar 95%. Tanaman kangkung mengalami pertumbuhan sebesar 47,86±2,81 cm dan 16,85±2,74 g.
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