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Eriza Juniar
Published: 5 July 2019
DENTA, Volume 12; doi:10.30649/denta.v12i2.159

Abstract:
Background: Tooth transposition is a rare and severe positional anomaly that may create many orthodontic problems from both esthetic and functional points of view. In this case report, the orthodontic management of distoversion upper central incisor and transposition of the upper right canine and lateral incisor. The patient was treated with a fixed appliance for 4 months. At this moment the central incisor has already corrected, lateral incisor has also erupted and aligned. Patient management should be continued with phase two treatment to correct the transposition of canine.Keywords: Distoversion, tooth transposition, orthodontic management.Correspondence : Departement of Pediatric Dentistry, College of Dentistry, University of Airlangga, Surabaya, East Java, Indonesia.
, Ilham Mahendra, Linda Rochyani
Published: 5 July 2019
DENTA, Volume 12; doi:10.30649/denta.v12i2.175

Abstract:
Backgrounds : Enterococcus faecalis is a facultative anaerobic gram-positive bacteria that known to have a role in the infection of the root canal, causing the failure of root canal treatment. The extract from anchovy (Stolephorus insularis) contains fluor has antibacterial properties that can inhibit the growth of gram-positive bacteria and has the potential to be developed as a root canal sterilization agent. Purpose : To determine the antibacterial effect of anchovy (Stolephorus insularis) extract to the Enterococcus faecalis. Methods : This study was an true experimental study with post test only control group design and were tested by diffusion methods, consisted of 5 groups: 2 control groups DMSO 1% as negative control and ChKM as positive control, 3 treatment groups of anchovy (Stolephorus insularis) extract with different concentrations of 18%, 24% and 30% which each group consisted of 5 samples. Antivbacterial effect was examined by measuring the clear zone around the filter paper. Data were analyzed by ANOVA test followed by LSD test. Results : Results of this study proved the presence of antibacterial effects of anchovy (Stolephorus insularis) extract to the Enterococcus faecalis. In this study, the higher concentrations used, the greater the antibacterial effect. Average inhibition zone of each groups: DMSO 1% (6,03 mm), ChKM (14,18 mm), 18%(6,83 mm), 24%(7,03 mm) and 30%(7,16 mm). Conclusion : Anchovy (Stolephorus insularis) extract has antibacterial effect to the Enterococcus faecalis.
Isidora Karsini, Farhana Nur Fadhila, Nafiah Nafiah
Published: 5 July 2019
DENTA, Volume 12; doi:10.30649/denta.v12i2.170

Abstract:
Background: Channa striata contains important compounds in the process of tissue synthesis and important role in wound healing, such as albumin, zinc (Zn), copper (Cu), iron (Fe) and unsaturated fat acids. Purpose : To determine the effect of Channa striata extract to the amount of neutrophil in healing process of traumatic ulcer. Material and Method : The design of this study used randomized post test only control group design. 18 wistar rats were divided into 6 groups, i.e: K-1 (no treatment for 1 day), K-3 (no treatment for 3 days), K+1 (giving hyaluronic acid 0.2% for 1 day), K+3 (giving hyaluronic acis 0.2% for 3 days), P1 (giving Channa striata extract 100% for 1 day), P3 (giving Channa striata extract 100% for 3 days). Results : There was a decrease in the number of neutrophil in the treatment group. The mean and standart deviation of the number of neutrophil in the K-1 group (75,96±3,65), K-3 (50,76±2,04), K+1(72,96±2,97),K+3(45,06±2,83),P1(54,20±1,31),P3(32,50±,85). Conclusion : The using of Channa striata extract 100% for 3 days are the most effective in decreasing the amount of neutrophil in healing process of traumatic ulcer of Rattus Novergicus Strain Wistar.
Published: 5 July 2019
DENTA, Volume 12; doi:10.30649/denta.v12i2.161

Abstract:
Background: Candidiasis is one of oral infectious disease caused by Candida albicans.The role of C. albicans as pathogen opportunistic in oral infection can affected by immune system. Phagocytosis has contributed in immune system against C. albicans infection, which is the role of monocytes. Grapthophyllum L. Griff has been proved to increase macrophage’s phagocytic activity, but the effect of violet leaves ethanol extract (EEDU) to increase monocyte’s phagocytic activity on C. albicans is unknown. Objectives: To determine the effect of violet leaves extract on monocyte’s phagocytic activity on C. albicans. Methods: This research was acted in vitro on human monocyte cell culture. There was 5 groups: negative control group, positive control group (incubated in Isoprinosine) and treatment groups was incubated in EEDU 2,5%, 5%, 10%. The percentage of activated monocytes was counted. Result: The result showed that incubation of EEDU 2,5%, 5% and 10% could increase monocyte’s phagocytic activity significantly (p<0,05) compared to control groups. The number of monocyte’s phagocytosis on C. albicans incubated with EEDU 2,5%, 5% and 10% is 65%, 60,5%, and 58,75%. The optimal concentration of EEDU to increase monocyte’s phagocytic activity was 2,5%. Conclusion: The study showed that EEDU can increase monocyte’s phagocytic activity on C. albicans. Keywords:Ethanol extract of Graptophyllum pictum L. Griff leaves, Monocyte’s Phagocytic Activity, Candida albicans
Yoifah Rizka Wedarti, Fani Pangabdian, Laurencia Isabella Loekito
Published: 5 July 2019
DENTA, Volume 12; doi:10.30649/denta.v12i2.174

Abstract:
Background: Porphyromonas gingivalis bacteria are found in dental plaques and have the ability to form biofilms. Biofilms are collections of bacterial cells, which are tightly attached to a surface covered by an extracellular polymer matrix released by the bacteria. Periodontitis therapy generally uses tetracycline antibiotics which if used long-term can cause resistance. Chitosan Rajungan crab extract (Portunus pelagicus) has antimicrobial potential which can be used as an alternative therapy. Objective: To determine the antibacterial power of Rajungan crab chitosan extract (Portunus pelagicus) in various concentrations of Porphyromonas gingivalis bacterial biofilms. Method: This research was true experimental laboratorist. This study used Rajungan crab chitosan extract (Portunus pelagicus) to Porphyromonas gingivalis biofilm tested by the test method biofilm. Divided into 5 groups, where each group consisted of 4 samples. The control group consisted of: K- (negative control group), K + (positive control group / tetracycline group), and 3 treatment groups, namely P1 (0.25% Rajungan crab chitosan), P2 (0.5% Rajungan crab chitosan) , P3 (1% Rajungan crab chitosan). Antibacterial power was determined by the value of Optical density in the ELISA Reader. Data analysis using One Way ANOVA followed by LSD test. Result: There were significant differences (p <0.05) seen from the percentage of biofilm inhibition in the negative control group for all groups, the positive control group for the crab crab chitosan extract concentrations of 0.25% and 0.5%. Conclusion: The chitosan crab chitosan extract (Portunus pelagicus) with 1% concentration has the greatest antibacterial effect on the Porphyromonas gingivalis biofilm
Agni Febrina Pargaputri, Dwi Andriani
Published: 5 July 2019
DENTA, Volume 12; doi:10.30649/denta.v12i2.164

Abstract:
Background: Oral candidiasis generally caused by Candida albicans with prevalence of 30-50%. C. Albicans proliferation in the oral cavity was canducted by decreasing immune system of the host. Lymphocytes activation in immune system happened when there is a contact between host cell with cell wall of C. albicans as a result of C. albicans antigen. One of therapy to suppress the Candida infection sistemically is by giving hyperbaric oxygen therapy. Purpose: To determine the effects of giving hyperbaric oxygen therapy to the amount of lymphocytes in oral candidiasis immunosuppressed models. Methods: This research was post test only control group design. We used 12 wistar rats which were divided into 3 groups(n=4/3): K-(normal/ healthy wistar rats), K+(oral candidiasis immunosuppressed wistar rats), P1(oral candidiasis immunosuppressed wistar rats which were given hyperbaric oxygen therapy 5 days). K+ and P1 groups were immunosuppressed by giving dexamethasone 0,5mg/day/rat orally for 14 days, added with tetrasiklin 1 mg/day/rat. On the 3rd day immunosuppression, the rats were inducted with C.albicans smeared in the dorsum linguae once every two days for 12 days. Results: The data were analyzed with one way Anova test and showed significant difference among groups (p<0,05). To show the difference between each groups we used LSD test and showed significant difference (p<0,05) between K+ compared with P1, and K+ compared with K-. Conclusion: Hyperbaric oxygen therapy gives effect to the amount of lymphocytes in oral candidiasis immunosuppressed models. Keywords: Oral candidiasis, immunosuppressed, hyperbaric oxygen therapy, lymphocytes Correspondence: Agni Febrina Pargaputri, Department of Oral Biology Faculty of Dentistry, Jl. Arif Rahman Hakim 150, Surabaya, Indonesia. Phone: 031-5945864, fax: 031-5912191, Email: [email protected]
, Enjel Arjuna Susru Wardana, Syamsulina Revianti
Published: 5 July 2019
DENTA, Volume 12; doi:10.30649/denta.v12i2.176

Abstract:
Background : The denture base made of acrylic resin has a porosity and water absorbtion characteristic to cause the deposition of food waste, that problem was known to cause a growth of Candida albicans microorganisms. This problem can be solved by providing a coating (Surface coating) made of natural polymer (cellulose acrtate) of sea sea grass (Enhalus acoroides) mixed with hydrocoloid material so as to provide protection properties. Such protective properties can inhibit the bonding between the residual monomer results of acrylic resins and microorganisms. Objective : To analyze and compare the number of Candida albicans colonies on the acrylic resin plate that has surface coating and no surface coating. Method : Using post test only group design, using 12 samples divided into 2 groups. Group 1 is the control group (Heat cured acrylic plate contaminated with Candida albicans). Group 2 : Treatment group (Heat cured acrylic plate with surface coating made from sea grass Enhalus acoroides then contaminated with Cndida albicans). Calculating the number of colonies from Candida albicans was done by calculating the turbidity of SDB media (Saboraud Dextrose Broth) using UV-VIS Specrofotometer with 560 λ wave. Data analysis using an Independent T-Test. Results : Acrylic resin plates with surface coating showed significant differences in the number of Candida albicans colonies. The average results in the control group obtained an average number of colonies of 1,58 CFU/ml, while in the treatment group as much as 1,42 CFU/ml. Conclusion : The results show that surface coating can decrease the amount of Candida albicans colonies because it can close the microporosity, prevent a water absorption, and prevent the formation of the pellicle.
Dyah Lestarining R, , Widyastuti Widyastuti
Published: 29 April 2019
DENTA, Volume 11; doi:10.30649/denta.v11i2.100

Abstract:
Background: Periodontitis is complication of DM in chronic state. Periodontitis susceptibility increase threefold in patient DM compared non-DM. Debridement with SRP alone is less effective in DM. Hyperbaric Oxygen Therapy (HBO) has the effect of increasing insulin sensitivity and synthesis of collagen. Collagen is the largest extracellular matrix supporting periodontal tissue. Stchopus hermanii contains condrotin sulfate serves to inhibit the formation of mixed periodontopatogen bacteri. Purpose: To determine the therapeutic effects of HBO 2,4 ATA 3x3’ interval 5’ 7 days and stichopus hermanii against periodontal ligament collagen density in the healing process of periodontitis with DM. Materials and Methods:The experiment design is factorial design. Rats are inducted with STZ 65 mg/kgBB to make them suffer DM and are inducted with P.gingivalis 1x109sel/ml for 5 days straight to make them suffer periodontitis, 20 male Wistar rats were divided into 4 groups. K0 (control group), K1(STZ+P. gingivalis), K2(STZ+P. gingivalis+Stichopus hermanii), K3(STZ+P. gingivalis+HBO), and K4(STZ+P.gingivalis+stichopus hermanii HBO) after treatment on day 52, all groups were sacrificed and done making preparations for the HPA of the mandible bone. Results: Data were analyzed using chi-square, Kruskal wallis, and Mann Whitney U with 95% significance level (p = 0.05). increase level of collagen significantly K1(1 ± 0.00) - K2(2±0.00), dan K1(1 ± 0.00) - K4(2.5 ± 0.289).Conclution:Therapy HBO combination of 2.4 ATA 3 x 30 with 5 minutes air breaks for 7 days and gel Stichopus hermanii3% effective on the Densityof collagen in the periodontal ligament healing process periodontitis with DM. Keywords:OHB, Stichopus hermanii, periodontitis with DM, collagen Correspondence: Dian Mulawarmanti, Department of Oral Biology, Faculty of Dentistry, Hang Tuah University,Arif Rahman Hakim 150, Surabaya,Phone 031-5945864, Email: [email protected]
Published: 29 April 2019
DENTA, Volume 11; doi:10.30649/denta.v11i2.99

Abstract:
In early 2010 there was a very rapid progress in the development of immunity to fungi, i.e the discovery of CLR. CLR is one of the PRR receptors found in immune cells that recognize the cell wall of fungi and then trigger a transduction signal that eventually provoke the production of various proinflamatory include IL1β, IL6, TNF α and induce polarization Th17. In the condition of infection the number of innate immune cells increases dramatically, lead to increasing of cytokines produced. Both cytokines and the cells that produce them enter the bloodstream. If there is unbalance level of pro and anti inflammatory cytokine, various inflammatory and/or autoimmune diseases can be occur. Unlike the focal concept infection, where the agent will cause disease if enters the blood vessels, through this concept a fungal infection at epithelium can lead to inflammatory diseases in other part of body without having to go through fungus Keywords: C. albicans, CLR, Cytokine, Inflamatory Disease Correspondence: Erna Sulistyani, Department of Oral Medicine, Faculty of Dentistry, Jember Bagian Penyakit Mulut, Fakultas Kedokteran Gigi, Universitas Jember, Kalimantan 37 Jember, Email: [email protected]
Published: 29 April 2019
DENTA, Volume 11; doi:10.30649/denta.v11i2.101

Abstract:
Introduction:The Dental Public Health Warrior (DPHW) project is a social activity which provides a creative, fun, and interactive dental health education throughout elementary schools in Keputih Area-Surabaya,Indonesia. Purpose: The aim of this study was to determine the effectivity of those projects in increasing the Performance Treatment Index of elementary school’s student. PTI score used to measure the willingness to treat their teeth. Materials and Methods:In this pre experimental study (pretest and posttest only design), subjects were 54 elementary students (4th grade) . All subjects were educated by the DPHW team and their DMF-t score and PTI score were collected by calibrated dentists before the education and in 3 months after the education. Results: The mean of DMF-t score increase from 6,7 to 6,9. The mean of PTI score increase from 3,7 to 9,5 . Wilcoxon analysis showed there is significant different between before and after education (p= 0,001). Conclusion:. The DPHW project can be effectively used to increase the Performance Treatment Index of elementary school’s student. The increase of PTI indekx means there is increase in willingness to treat their teeth. Keywords: Dental Public Health Warrior Project, PTI score , Performance Treatment Index,elemntary school, Surabaya. Corrrespondence:Aulia Dwi Maharani, Department of Public Health, Faculty of Dentistry, Hang Tuah University,Arif Rahman Hakim 150, Surabaya,Phone 031-5945864, Email: [email protected]
Kristin Gaby Rosari, , Lisdiana Mardanus
Published: 29 April 2019
DENTA, Volume 11; doi:10.30649/denta.v11i2.95

Abstract:
Background:Maxillary expansion uses mechanical forces to open the maxillary suture which causes inflammation and bone remodeling around the area. Stichopus hermanni is known to contain flavonoid, chondroitin sulfate, and saponin which is used to inhibit inflammatory process, increase bone metabolism and mineralization, and wound healing. Purpose: The aim of this experiment is to know the effect of Stichopus hermanni to bigonial width on maxillary suture remodeling expansion using cephalometric analysis. Materials and Methods: Experimental units used were 24 male Cavia cobaya divided into 4 groups (n=6). Group K(-) is negative control. Helical spring was applied and activated in the other 3 groups for 10 days. Group K(+) was given 2% NaCMC gel, both group P1 and P2 was given 3% Stichopus hermanni gel. Group P2 were also given retention period for 10 days after activation period. Cavia cobaya were then decapitated and observed for the bigonial width changes. The data was analyzed with One Way ANOVA test continued with LSD test. Results: The result of ANOVA test showed siginficant differences among the treatment groups p=0,000 (p<0,05). LSD test showed significant differences between group K(-) and K(+) (Sig. 0,005), K(-) and P1 (Sig. 0,001), and group K(-) and P2 (Sig. 0,000). Conclusion: 3% Stichopus hermanni gel increases bigonial width but not significant in remodeling process on maxillary suture expansion. Keywords: Stichopus hermanni, bone remodeling, maxillary suture expansion, bigonial width, cephalometric analysis Correspondence: Noengki Prameswari, Department of Orthodontics, Faculty of Dentistry, Hang Tuah University, Arif Rahman Hakim 150, Surabaya, Phone 031-5945864, 5912191, Email: [email protected]
Published: 29 April 2019
DENTA, Volume 11; doi:10.30649/denta.v11i2.102

Abstract:
Background : A common objective of anterior restorative dentistry is to establish anterior guidance between the maxillary and mandibular anterior teeth to disclude the posterior teeth during protrusive mandibular movement. Fracture anterior teeth leads to psychological trauma, along with functional and esthetic debilitations. Healthy anterior teeth play an important role of protecting the posterior teeth during excursive mandibular movement. The contour and shape of the labial surface are important for esthetic purpose; the palatal surface morphology is critical for harmonious function. Premature contact in palatal surface restoration is considered as contributing factor to temporomandibular disorder (TMD) symptoms. Purpose : Improve aesthetics and function of the patient's anterior teeth. Case : Male, 17 years old with poor aesthetic because of fracture anterior teeth 4 years ago. Case Management : Root canal treatment, treatment of root resorption, fiber post, custom post, indirect veener, zirconia crown and enameloplasty. Result : Better aesthetic and get the optimum function of his teeth. Keyword: Aesthetic treatment, anterior fracture, zirconia crown, indirect venner Correspondence: Sinta Puspita, Department of Conservative, Faculty of Dentistry, Hang Tuah University, Universitas Hang Tuah, Arif Rahman Hakim 150 Surabaya, Phone 031-5945864, 5912191, Email: [email protected]
Rafika Rusydia Darojati, Yoifah Rizka, Syamsulina Revianti
Published: 29 April 2019
DENTA, Volume 11; doi:10.30649/denta.v11i2.94

Abstract:
Background: Diabetes mellitus (DM) is a metabolic disorders characterized by hyperglycemia caused by oxidative stress. Porphyromonas gingivalis (P.g) is a periodontopathogen caused periodontitis exacerbate blood glucose levels (BGL). Hyperbaric oxygen therapy (HBOT) combined with stichopus hermanii (SH) has a role as an antioxidant and antibacterial agent. Purpopse: To analysis effect of HBOT, SH powder and combination of both to the level of blood glucose diabetic Wistar rat induced P.g. Materials and Methods: This study was pre-test-post-test control group design, using 20 male Wistar rat were divided into 5 groups, Group1 (STZ), Group2 (STZ+P.g), Group3 (STZ+P.g+HBOT), Group4 (STZ+P.g+SH powder), Group5 (STZ+P.g+HBO+SH). STZ induction intravenous for 4 days in a row. P.g. induction was exposure 3 times for 4 days. After therapy (7 days), sample blood tail vein of rat were taken for measure BGL by glucometer. Result: Level of blood glucose K1(280.25±88.786)mg/dl, K2(372.25±135.662)mg/dl, K3(249.50±96.652)mg/dl, K4(172.00±82.077)mg/dl, K5(104.75±38.257)mg/dl. Conclusion: Induction of STZ increased the BGL but induction of P.g. cannot exacerbate BGL . HBOT combined with SH powder can reduce BGL in STZ and P.g. induction group.Keywords: Diabetes mellitus, periodontitis, blood glucose levels, Stichopus hermanii, hyperbaric oxygen.Correspondence: Yoifah Rizka, Department of Periodontology, Faculty of Dentistry, Hang Tuah University, Arif Rahman Hakim 150, Surabaya, Phone 031-5945864, 5912191, Email address: [email protected]
, Vivin Ariestania
Published: 29 April 2019
DENTA, Volume 11; doi:10.30649/denta.v11i2.97

Abstract:
Background: Heat cure acrylic and nylon thermoplastic valplast and lucitone-FRS brands are the three materials most often used as denture bases. Candida albicans is the dominant microorganism that can cause denture stomatitis. Cleaning denture can be done in two ways, namely the mechanical way with a toothbrush or an ultrasonic cleanser and a chemical method by immersing the denture into the cleaning solution. Chitosan solution is antibacterial and anti fungal which can inhibit the growth of Candida albicans colonies on heat cured acrylic plates, valplast and lucitone-FRS. Purpose: To determine the concentration of chitosan solution and the type of denture plate that most effectively inhibited the growth of Streptococcus mutans Candida albicans colonies on heat cured acrylic, valplast and lucitone-FRS plates. Materials and Methods: 21 heat cured acrylic plate samples, 21 valplast plate samples, 21 lucitone-FRS plate samples measuring 10x10x2 mm divided by 9 groups. Heat cured, valplast and lucitone-FRS acrylic plate samples were contaminated with Candida albicans then immersed using the concentration of 0.25%, 0.5% chitosan solution and sterile aquades as the control group with 90 minutes immersion time. The entire study sample was calculated using Candida albicans colonies on Sabourroud's Dextrose Agar media. Results: The Kruskall Wallis test showed a significant difference (p <0.05) in all treatment groups. Mann-Whitney showed a significant difference (p <0.05) in all groups in the Candida albicans study. Conclusion: Soaking the lucitone-FRS plate in 0.5% chitosan solution for 90 minutes was the most effective in inhibiting the growth of Candida albicans colonies. Keywords:Kitosan, Candida albicans, akrilik heat cured, valplast, lucitone-FRS Correspondence: Anindita Apsari, Department of Prosthodontics, Faculty of Dentistry, Hang Tuah University, Arif Rahman Hakim 150, Surabaya, Email : [email protected]/[email protected]
Arlita Gladys Tricia Charyadie, Aprilia Aprilia, Widyastuti Widyastuti
Published: 29 April 2019
DENTA, Volume 11; doi:10.30649/denta.v11i2.92

Abstract:
Background: Dental caries is a major oral health problem which is most commonly found in Indonesia. One of the main factors in role of causing dental caries is Streptococcus mutans which is the primary target of the prevention of caries. Leaf extract of Sonneratia alba hasbeen proven to have an antimicrobial effect towards Streptococcus mutans, but its cytotoxic activity on normal cells is still unknown. The initial cell viability in this study was conducted using cell line BHK-21. Purpose: The aim of this study is to determine the cell viability offibroblast cells line BHK-21 by given Sonneratia alba’s leaf extract. Materials and Methods: The experiment was conducted using post test only control group design. BHK-21 fibroblast cell culture on 96 wells were divided into cell control group (n=9), media control group (n=9) and test groups (n=9). The test groups were given various concentration of Sonneratia alba’s leaf extract, they were: 5%; 10%; and 20%. The cell cultures were incubated for 24 hours before and after treatment using Sonneratia alba’s leaf extract. After dripping MTT into the microplates, OD were read using ELISA reader and the viability were counted. The data that was acquired from the viability calculation was statistically analyzed using One Way ANOVA and LSD test. Results: Data showed the increase of viability cell in all the test groups. The highest viability cell showed by the test group with 10% concentration of Sonneratia alba (153,7122%±7,42458), and the lowest found in the test group with 20% concentration of leaf extract (141,4682%±7,48752). Conclusion: In this experiment, the leaf extract of Sonneratia alba proved to be nontoxic on fibroblast cells line BHK-21, the extract increased the viability and also caused proliferation of fibroblast cells. Keywords: Dental caries, Sonneratia alba, fibroblast, viability, cell cultureCorrespondence: Aprilia, Department of Conservation, Faculty of Dentistry, Hang Tuah Univesity, Arif Rahman Hakim 150, Surabaya, Phone 031-5945864, Email: [email protected]
Faraziza Maulana, , Fanny M. Laihad
Published: 29 April 2019
DENTA, Volume 11; doi:10.30649/denta.v11i2.93

Abstract:
Background: Periodontitis is the most severe complication in oral cavity to diabetes mellitus. Stichopus hermanii contain GAG, a polysaccharide which very useful in wound healing. Hyperbaric oxygen therapy is effective repairing ischemia, hypoxia, and is used as adjuvant therapy. Purpose: To determine effect of administration of Stichopus hermanii (SH) 3% gel and hyperbaric oxygen therapy 3x30 minutes with 5 minute intervals in 7 consecutive days to the number of fibroblasts in ligament periodontal diabetes mellitus rats with periodontitis. Materials and Methods: The experiment design is factorial design. Rats are inducted with STZ single dose 65 mg/kgBB to make them suffer DM and are inducted with P.gingivalis 1x109sel/ml for 5 days straight to make them suffer periodontitis. 20 male wistar rats are divided into 5 groups. K- (control), K1 (STZ+P.Gingivalis), K2(STZ+P.Gingivalis+SH), K3(STZ+P.Gingivalis+HBO), K4(STZ+P.Gingivalis+SH+HBO). After 52 days treatment, rats is terminated to make a HPA Sample from the mandible and fibroblast is counted with 400x magnification. Result: One-way Annova here were significant differences (p<0,05) in K- (32.300 ± 11.381), K1 (31.725 ± 5.590), K2 (54.225 ± 7.165), K3 (49.725 ± 10.088), K4 (72.075 ± 9.342). Conclusion: administration of Stichopus hermanii 3% gel combination and 2.4 ATA hyperbaric oxygen 3x30 minutes a day with 5 minutes intervals in 7 consecutive days can increase the number of fibroblasts in diabetes mellitus rats with periodontitis. Keywords: Stichopus hermanii, hyperbaric oxygen, diabetes, periodontitis, fibroblasts Correspondence: Dian Mulawarmanti, Department of Oral Biology,Faculty of Dentistry, , Hang Tuah University, Arif Rahman Hakim 150, Surabaya, Phone 031-5945864, Email: [email protected]
Yulie Emilda Akwan, Ayulistya Paramita, Eriza Juniar
Published: 29 April 2019
DENTA, Volume 11; doi:10.30649/denta.v11i2.104

Abstract:
Background: Oral habits include habit which are continuously done and has the potential to cause defects on teeth and perioral tissues.Lip sucking or biting habits are often found simultaneously or as a substitution of finger sucking. This habit may affect the labial and perioral structures. The habit may take several forms. Two extreme types are mild wetting of the lips with the tongue and pulling the lips into the mouth between the teeth. The lip bumper is a functional device that is used successfully to intercept developing dental and occlusal problems by allowing a proper development of the arch length and width for eruption of the permanent teeth.Purpose: This study aimed to re-validate the effectiveness of lip bumper application and habitual therapy in treating the bad habit of lip sucking. Case Report: The mother of a 9-year-old girl reported an upper front teeth protrusion and frequent occurrence of mouth ulcer. Further examination revealed that the patient had a habit of lip sucking. Case Management: Removable Lip Bumper is used to control lip habits such as lip sucking and to improve mentalis muscle hyperactivity. Such habit was treated with the use of a removable lip bumper and habitual therapy conducted by both parents. Constants reminder and encouragement from the parent to wear the lip bumper is one of the key success factors in this treatment. Conclusion: Five months after the initial application of a lip bumper and habitual therapy, the patient overcame the habit of lip sucking.Keywords: lip bumper, lip sucking, myofunctional therapyCorrespondence: YulieEmildaAkwan, Department of Pediatric Dentistry, Faculty of Dentistry, Hang TuahUniversity, Arif Rahman Hakim 150, Surabaya, Phone 031-5945864, Email: [email protected]
Ajeng Hanun Winny Khairina, , Nafi’Ah Nafi’Ah
Published: 29 April 2019
DENTA, Volume 11; doi:10.30649/denta.v11i2.96

Abstract:
Background: Oral candidiasis is common opportunistic infection in oral cavity caused by the fungal pathogen Candida albicans and it can develop to malignant such as a candida leukoplakia if it’s affected by cigarette smoke exposure. Purpose: To analysis the effectivity of Stichopus hermanii supplementation of catalase activity in Wistar rats submandibular with oral candidiasis. Materials and Methods: This study was post-test-only control group design, using 35 male Wistar rats were divided into 5 groups, Group1 is negative control, Group2 is positive control, Group3 is treatment control with Stichopus hermanii 0,0225 mg/g BB powder, Group4 is treatment control with Stichopus hermanii 0,045 mg/g BB powder) Group5 is treatment control with Stichopus hermanii 0,09 mg/kg BB powder). Oral candida infection was achieved by spray and cotton swab rolled over all parts of the mouth. After treatment, rats were euthanized and submandibular were biopsied to make histopathology slide for measure activity of enzim catalase with spectrophotometer λ 280 nm. Data were analyzed using One Way ANOVA and Tukey LSD test. Results: There are significantly differences catalase activity between K1&K2, K2&K3, K4&K1, K2&K4, K2&K5. Conclusion: Suplementation of Stichopus hermanii effectiv of catalase activity in wistar rat’s submandibular with Candida albicans A infection. Keywords: Oral candidiasis, catalase activity, Stichopus hermanii, Candida albicans Correspondence: Syamsulina Revianti, Department of Oral Biology, Faculty of Dentistry, Fakultas Kedokteran Gigi, Universitas Hang Tuah, Arif Rahman Hakim 150 Surabaya, Telepon 031-5945864, 5912191, Email: [email protected]
Dianty Saptaswari, Widyatuti Widyatuti, Yoifah Rizka Wedarti, Hansen Kurniawan
Published: 29 April 2019
DENTA, Volume 11; doi:10.30649/denta.v11i2.98

Abstract:
Background: Dental implant is a device that is surgically implanted into the soft tissue or into the jaw bone. dental implants have become the treatment of choice for replacing missing teeth. Platelet rich plasma and platelet concentration derived from autologous blood containing autologous growth factors in which the platelet rich plasma can improve wound healing and increase the regeneration of bone. today cone beam computed tomography (CBCT) has been available for 3D visualization of craniofacial section. CBCT 3 D is able to provide information on the general bone mineral density. Purpose: The purpose of this studywas to determine thebone mineral density by three-dimensional radiographic picture after dental implant placementbyan additional plateletrich plasma. Material and Method: This study was divided into 2 groups. The first placement of a dental implant with platelet rich plasma and the second placement of a dental implant without platelet rich plasma. Results: there are significant differences in dental implant placement with platelet rich plasma and placement of dental implants without platelet rich plasma. In the test results obtained Independent T test (p = 0.02). Conclusion: There was an increase bone mineral density in dental implant placement with platelet rich plasma were performed using three-dimensional radiographic examination (CBCT).Keyword: Dental implant, platelet rich plasma, CBCT, bone densityCorrespondence: Dianty Saptaswari, Widyatuti, Yoifah Rizka Wedarti, Hansen Kurniawan Periodonsia Fakultas Kedokteran Gigi Universitas Hang Tuah Surabaya, Email : [email protected]
Annisa Listya Paramita
Published: 29 April 2019
DENTA, Volume 11; doi:10.30649/denta.v11i2.103

Abstract:
Background: Nursing bottle carries is a problematic condition in children. On one side, parents try to supply enough nutrition to their children, though on the other side this night-time bottle feeding habit might cause negative effect on the childrens dental health. Esthetic dental treatment on children have improved greatly over the past few years, as parents expect an almost-complete restoration of their child’s dental condition. The alternatives for pediatrics esthetic dental restoration may depend on the cooperativeness and habit of the child themselves, while also taking the child’s development into consideration. Aim: to report evaluation of aesthetic restoration on primary teeth. Case: A 4 year-old female child was presented by her parents, with damaged four anterior teeth, which made her uncomfortable especially on feeding due to the sharp edges. From history taking we found a night-time bottle-feeding habit, which usually occurred until patient was asleep. Case Management: restoration of the four primary incisive teeth was performed using a resin composite crown. Outcome of the treatment was evaluated until the primary teeth exfoliateand permanent teeth arise. Conclusion: cooperation between patient, parents, and dentist is important in the dental restorative treatment on paediatric patient. Moreover, a good communication with parents is encouraged, especially on follow-up schedule, treatment cost, success rate and possibility of treatment failure is also encouraged. Staged evaluation is needed due to the natural substitution of the primary teeth by permanent teeth, besides the development process of the jaws.Keywords: Nursing bottle caries, esthetic restoration, growthanddevelopmentCorrespondence: Annisa Listya Paramita, drg., Sp.KGA. Departemen Ilmu Kedokteran Gigi Anak. Fakultas Kedokteran Gigi Universitas Hang Tuah, Surabaya. Jl. Arif Rahman Hakim No. 150, Surabaya.
Arya Barahmanta, Agni Febrina Pargaputri, Jessica Jenuary Yasin
Published: 1 February 2019
DENTA, Volume 13; doi:10.30649/denta.v13i1.179

Abstract:
Background: The prevalence of malocclusion in Indonesia reaches 80%, causing orthodontic treatment needs to increase. Orthodontic treatment takes a long time around 15-24 months so additional therapy is needed to accellerate the orthodontic treatment process. Hyperbaric oxygen therapy (HBOT) and Stichopus hermanii (golden sea cucumber) which contain bioactive ingredients can work synergistically to reduce inflammatory cytokines. Purpose: To determine the effect of HBOT and Stichopus hermanii on the number of macrophages in periodontal ligament of pressure and tension area in the process of orthodontic tooth movement. Material and Methods: This research used Randomized Post Test Only Control Group Design. A sample of 30 marmot was randomly divided into 5 groups: negative groups (K-), positive groups (K+), Stichopus hermanii treatment group (P1), HBOT group (P2), and combination (P3). On K+ and treatment groups, maxillary incisors of marmot is installed helical spring in order to move the teeth to distal. Analysis of the number of macrophages histologically using Hematoxylin Eosin (HE) staining. And then tested using the Kruskal-Wallis test, and the Mann Whitney Wilcoxon test. Result: The research data showed a decrease of mean average number of macrophages in the pressure area, K (-): 1.90 ± 0.41; K (+): 4.40 ± 1.14; P1: 4,30 ± 0,90; P2: 3.60 ± 0.54; P3: 2.90 ± 0.41 and the mean number of machrophages in tension area also descreased, K (-): 2.00 ± 0.35; K (+): 4.00 ± 1.00; P1: 4,30 ± 0,27; P2: 4,10 ± 0,65; P3: 3.60 ± 0.65. Conclusion: The combination of HBOT and Stichopus hermanii decreases the number of macrophages in the pressure and tension area in orthodontic tooth movement.
Pambudi Rahardjo, Maria Lisdiana Tandjung, Dandy Bayu Angkasa
Published: 1 February 2019
DENTA, Volume 13; doi:10.30649/denta.v13i1.183

Abstract:
Background: The mechanical force of orthodontic device causes occurrence of a tension and a pressure area in the periodontal ligament. In the tension area, an inflammatory response characterized by vasodilation of blood vessels will occur. The function of blood vessel is to flow blood and other nutrients to accelerate the periodontal ligament remodeling process, so that relapse can be prevented. Objective: To determine the effect of combination of propolis 3%, 5% for 14 days and HBOT 2,4 ATA 3x30 minutes with 5 minutes interval for 7 days in the tension area of the periodontal ligament as an effort to prevent relapse. Materials and Methods: A randomized post-test only control group design was used in this study. 42 male guinea pigs (cavia cobaya) are divided into 7 groups K- (without treatment), K + (using separator), P1 (using separator and propolis extract 3%), P2 (using separator and propolis extract 5%), P3 (using separator and hyperbaric oxygen therapy), P4 (using separator and combination of hyperbaric oxygen therapy and 3% propolis extract), and P5 (using separator and combination of hyperbaric oxygen therapy and 5% propolis extract) . Results: Data shows that mean of diameter of blood vessels increased from the smallest K- (41,8417±0,84511), K+(61,77±1,254482), P1(72,5983±0,68869), P2(73,2333±1,24481), P3(74,2583± 0,62030), P4(91,47±0,76585), to the largest in P5(91,5483±0,96178). One-way Anova and LSD test showed significant differences in blood vessels diameter (p <0.05). Conclusion: The combination of propolis 3% and 5% and HBOT have significant effect in increasing blood vessels diameter in the tension area of the periodontal ligament
Meinar Nur Ashrin, Widyasri Prananingrum, Firda Dean Yonatasya
Published: 1 February 2019
DENTA, Volume 13; doi:10.30649/denta.v13i1.177

Abstract:
Backgorund: Anadara granosa shells can be converted into calcium compounds in the form of hydroxyapatite (HA), tricalcium phosphate (TCP), and calcium carbonate (CaCO3) by hydrothermal method. These calcium compounds have the potential to increase the proliferation of fibroblast cells in the socket healing process. Purpose: The aim of this study was to determine the effect of calcium compounds from the synthesis of Anadara granosa with variations in sintering time against proliferation of fibroblast cells in socket healing. Methods: 36 Wistar rats were divided into control group (K1, K2), and treatment group (P1a, P1b, P2a, P2b) which was applied by bone graft with the sintering time 6 and 12 hours on the socket incisor of the left mandible. On the 7th and 14th days, the mandibular bone was cut transversely and stained. Observation of the number of fibroblast cells use 400x magnification with 3 visual fields and the mean was calculated. Data were analyzed using the Kruskal-Wallis test. Results: The mean number of fibroblast cells in groups K1, K2, P1a, P1b, P2a and P2b respectively is 23.50; 30,16; 28.94; 34.22; 30,16; and 37.50. There were significant differences in all groups, except K2-P1a, K2-P2a, and P1a-P2a. Conclusion: Calcium compounds bone graft from the synthesis of Anadara granosa with sintering time of 6 and 12 hours affect the proliferation of fibroblast cells in socket healing. Bone graft with a sintering time of 12 hours can increase fibroblast cell proliferation more effectively.
Paulus Budi Teguh, Linda Rochyani, Clarissa Valerie Wardojo
Published: 1 February 2019
DENTA, Volume 13; doi:10.30649/denta.v13i1.178

Abstract:
Background: Tooth loss can be replaced by the use of dentures. Cleaning of dentures by brushing using toothpaste can cause surface roughness because of abrasive materials. The use of toothpaste can be replaced with paste of extract Cymbopogon citratus because it has antibacterial and antifungal effects. Purpose: to determine the effect of brushing using paste of extract Cymbopogon citratus on the surface roughness of heat cured acrylic resin. Methods: Sample were heat cured acrylic resin (62.5 x 10 x 2.5) mm, divided into 4 groups consist of negative control group was brushed with basic paste, positive control group was brushed with herb paste, and two groups were brushed with paste of Cymbopogon citratus extract with 30% and 60% concentrations. Brushing was done for 3 minutes with 60 brushing times and then measured with surface roughness tester. The data was processed by ANOVA test. Result: The average surface roughness in K-, K+, P1, and P2 were 0,1217, 0,3433, 0,1633, and 0,27 μm. There was significant difference between negative control group and positive control group; negative control group with P1 and P2 group; positive control group with P1 and P2 group; P1 group with P2 group (P <0.05). Conclusion: The paste of Cymbopogon citratus extract concentration of 30% was effectively used because the level of surface roughness below the maximum limit of recommended surface roughness of dentistry material which is 0,2 μm. The 60% concentration paste of Cymbopogon citratus extract was less effective because the level of surface roughness above the maximum limit of surface roughness is recommended.
Nafiah Nafiah, Sarianoferni Sarianoferni, Rizqi Ananda Putri
Published: 1 February 2019
DENTA, Volume 13; doi:10.30649/denta.v13i1.180

Abstract:
Background: Pravalence of Traumatic Ulcers is quite high in Indonesia. Therapy that has been carried out is still symptomatic, now there are many herbal ingredients that can be used as an alternative ingredients for medicine, including spirulina platensis. Spirulina platensis is one type of biological wealth that is widespread in Indonesian marine waters, and has bioactive ingredients that are beneficial to the body and can help the wound healing process. Purpose: to determine the differences in the effectiveness of topical administration of spirulina platensis ethanol extract 96% and 70% against the density of collagen fibers in healing traumatic ulcers. Methods: The subjects of the study were 36 wistar rattus novergicus rats divided into 6 groups, treated with traumatic ulcers in the lower labial mucosa, treated according to their respective groups K1.3 (control group 3 days), P1.3 (Giving spirulina gel platensis ethanol extract 96% 3 days), P2.3 (Giving spirulina platensis ethanol extract 70% 3 days), K1.7 (Control group 7 days), P1.7 (Giving spirulina platensis ethanol extract 96% ethanol 7 days ), P2.7 Giving spirulina platensis ethanol extract 70% 7 days 7). Results: There were differences in the effectiveness of giving spirulina platensis ethanol extract 96% and 70%. There is a significant difference in the P1.7 group (score = 4) with P2.7 (score = 3), P1.3 (score = 3) with P1.7 (4), P1.3 (score = 3) with P2 .3 (score 3), K1.3 (score = 2) with P1.3 (score = 3) and P2.3 (score = 3), K1.7 (score = 3) with P1.7 (score = 4) ) and P2.7 (3). Conclusion: There was no difference in effectiveness between 96% and 70% spirulina platensis ethanol extract gel on day 3 and there was a difference in effectiveness between 96% spirulina platensis ethanol extract gel on day 7.
Sularsih Sularsih, Widaningsih Widaningsih, Dwi Ayu Trisnawati
Published: 1 February 2019
DENTA, Volume 13; doi:10.30649/denta.v13i1.184

Abstract:
Background: Scaffold can be use in bone regeneration, but it should have similarities characteristic to a natural bone such as compressive strength that supported the process of growth and development of bone. This research use scaffold that made from chitosan and Aloe vera. Chitosan well-known as osteoconductive material, meanwhile Aloe vera was osteoinductive material. Purpose: The aim of this research was to compare compressive strength scaffold combinations of chitosan and Aloe vera extracted water and ethanol. Methods: Research design using post test only control group design with 36 scaffolds divided into 6 groups. Group 1 was scaffold Aloe vera extracted water (AVa), group 2 was scaffold Aloe vera extracted ethanol (AVe), group 3 was scaffold combination chitosan and Aloe vera extracted water 1: 1 (k-AVa1), group 4 was the scaffold combination of chitosan and Aloe vera extracted ethanol 1: 1 (k-AVe1), group 5 was scaffold combination of chitosan and Aloe vera extracted water 1: 2 (k-AVa2), and group 6 was scaffold combination of chitosan and Aloe vera ethanol extract 1: 2 (k -AVe2). Compressive strength test used Universal Testing Machine (Shimadzu Autograph–AGS–1kNX and using software Trapezium X). Data were analyzed using One Way ANOVA and Games Howell test. Results: The result of compressive strength AVa=1.36 MPa, AVe=1.03 MPa, k-AVa1=0.27 MPa, k-AVe1=0.11 MPa, k-AVa2=0.38 MPa, and k-AVe2=0.29 MPa. All groups have significant differences except AVa & AVe and k-AVa1 & K-AVe2 (p>0,05). Conclusion: There was difference between compressive strength scaffold combinations of chitosan and Aloe vera extracted water and ethanol, and the most highest compressive strength is AVa.
Dwi Andriani, Diana Soesilo, Ghora Setyawan
Published: 1 February 2019
DENTA, Volume 13; doi:10.30649/denta.v13i1.182

Abstract:
Background: Corticosteroid has a strong anti-inflammatory effect, unfortunately long-term usege of corticosteroid drugs can suppressed immune system. This condition makes Candida albicans as an opportunistic phatogen caused Oral Candidiasis. Macrophages have an important role in the first defense against infection. Methanol extract of A.ilicifolius leaf has anti-fungal, anti-inflammatory compounds to fight the growth of Candida albicans. Purpose: To determine the effect of methanol extract of A.ilicifolius leaf theraphy to the amount of macrophages in the healing pricess of oral candidiasis models with immunosuppressed conditions. Method: This study was true experimental with post test only control group design. Thirty-six strain wistar, 3 months old, and 250 grams of weight which were given dexamethason 0.5 mg and tetracycline 0.5mg for 7days, after Candida albican(ATCC10231) was induced for 2 weeks (3 times/week). Rats were divided into 6 groups: untreated(K-), nystatin(K+), A.ilicifolius 8%(P1), A.ilicifolius 12%(P2), A.ilicifolius 16%(P3), A.ilicifolius 20%(P4 ) all groups were treated for 2 weeks. After being treated, the rats tongue biopsied to examine the macrophages with Haematoxylin-eosin staining and observed with microscope (400x magnification). Data analyzed statistically by One-way ANOVA. Results: the amount of macrophages at K+(47.60±4.16), P4(40.20±3.56), P3(36.20±1.92), P2(17.00±2.45) were greater than K-(15.60±4.56), P1(14.60±2.70). there are no significant difference between group K- with P1 and P2, P3 with P4. There are significant difference between group K- with K+, K- with P3 and P4. K+ with P1, P2, P3 and P4. Group P1 with P3 and P4. Group P2 with P3 and P4. Conclusion: A.ilicifolius extract can increase the amount of macrophages in oral candidiasis immunosuppression models
Eddy Hermanto, Soemartono Soemartono, Dwi Surya Sudrajat
Published: 1 February 2019
DENTA, Volume 13; doi:10.30649/denta.v13i1.181

Abstract:
Background: Sockets healing is a dynamic, continuous and overlapping process. Osteoblasts are important cells in healing sockets that will mineralize into bone until the injured tissue heals. Lemuru fish oil (Sardinella longiceps) contains omega 3 which can increase the number of osteoblasts so that it accelerates the process of socket healing after tooth extraction. Objective: To determine the effect lemuru fish oil that given systemically in increasing the number of osteoblasts in post-extraction tooth socket wound healing. Materials and methods: Experimental research laboratories with post test only control group design. Samples of 33 male Wistar mice were divided randomly into 3 groups. Group K as a control group that was not given lemuru fish oil, group P1 as a group given lemuru fish oil 1.5ml / 200g BB, and P2 group as a group given 1 ml / 200g BB lemuru fish oil. Extraction of left mandibular first incisor was carried out, then extract was given once a day for 7 days with gastric sonde. Observations were made on the 14th day after tooth extraction by calculating the number of osteoblasts in the histology sample using HE (Hematoxylin Eosin). Results: In each treatment group there were significant differences (P 0, 05). Conclusion: Giving lemuru fish oil can increase the number of osteoblasts to post-extraction tooth socket wound healing.
, Paramita Devi Oktaviani, Dwi Andriani
Published: 1 February 2018
DENTA, Volume 12; doi:10.30649/denta.v12i1.155

Abstract:
Background: Candida albicans is normal flora of oral cavity that can be pathogenic due to predisposition influence so that trigger Oral candidiasis. Stichopus hermanii suspected as an Oral candidiasis therapy because it contain antioxidant compound, antitumor and antifungi. Objective: Analyzing the effectiveness of Stichopus hermanii supplementation as a protective effect of oral candidiasis in Rats exposure to smoke. Materials and Methods: This experiment is post test only group control design using 35 male rats divided into 5 groups(X). X1 (negative control), X2 (positive control), X3 (Stichopus hermanii powder 0,0225mg/kgBB), X4 (Stichopus hermanii powder 0,045mg/kgBB), X5 (Stichopus hermanii powder 0,09mg/kgBB). Candida albicans induced into the mouth by an oral swab using cotton bud 3 times a week for 8 weeks. Smoke exposure as a predisposition factor be given 3 bars per day for 8 weeks. The rats being killed and tongue biopsies measure the thickness of tongue epithelium. The obtained data analyzation using One Way ANOVA and LSD test. Result: There is a significant difference (p<0.05) between X1 and X2, X1 and X3, X1 and X4, X1 and X5, X2 and X3, X2 and X4, X2 and X5, X3 and X5. Conclusion: Adduction of Stichopus hermanii capable of reducing risk of Oral candidiasis. Supplementation of Stichopus hermanii powder 0,09mg/kgBB is the most effective reduce thickness of the tongue epithelium in the group that exposure to smoke and induced of Candida albicans.Keywords: Stichopus hermanii, Oral candidiasis, Tongue, Candida albicans, Thickness of Epithelium Correspondence: Endah Wahjuningsih, Laboratorium Biologi Oral Fakultas Kedokteran Gigi Universitas Hang Tuah, Jl, Arif Rahman Hakim 150, Surabaya, Indonesia. Ph 031-5945864, fax: 031-5912191, e-mail address: [email protected]
Budi Handayani, Arya Brahmanta
Published: 1 February 2018
DENTA, Volume 12; doi:10.30649/denta.v12i1.160

Abstract:
Background : relapse is often happen after orthodontic treatment finished. Propolis contains flavonoid, saponin and CAPE that known increase the number of osteoblast. Osteoblast have important role in bone remodeling which can prevent relapse. Purpose: to determine increasing number of osteoblast in bone remodeling after the giving 3% and 5% of propolis to prevent relapse after orthodontic treatment. Materials and metode: the design of this study was randomized post testonly control group design. 28 guine pig were divided into 4 groups of K- (without treatment), K+ ( giving separator rubber), P1( giving separator rubber and 3% propolis), P2 ( giving separator rubber and 5% propolis). Result : There are increasing number of osteoblast in the tension side of alveolar bone in treatment group. The average number of osteoblast in the tension side of alveolar bone in group K-:26.86; K+:33,29; P1:41.29; P2:48.29. Conclusion : giving 5% of propolis is the most effective I increasing the number of osteoblast in tension side of alveolar bone in relapse after orthodontic treatment.Keyword : osteoblast, orthodontic, propolis,Correspondence: Departement of Orthodontics, Faculty of Dentistry, Hang Tuah University, Arif Rahman Hakim 150, Surabaya, Phone 031-594864, Fax 031-5912191, Email: [email protected]
, Ratna Putri, Henu Sumekar
Published: 1 February 2018
DENTA, Volume 12; doi:10.30649/denta.v12i1.157

Abstract:
Background: The cleaning and shaping of the root canal are important stages in root canal treatment. The irrigation solution needed in those stages to clean the canal from residual necrotic tissue, demtine particles, and microorganism. Irrigation solution must fulfill some criteria among others, able to solute the debris or tissue residual, has low surface tension and not toxic. Mangrove Daruju (Acanthus ilicifolius) has potency to be a alternative of the usual irrigation solution because has antibacterial effect. Purpose: The aim of this study was to examine the cytotoxicity of Daruju mangrove (Acanthus ilicifolius) as root canal irrigation against the fibroblast cell (BHK-21) culture. Methods: The samples used was fibroblast cells (BHK-21) using culture method. These samples were treated with Acanthus ilicifolius chloroform extract with several concentration. Samples were divided into 1: cell control, 2:media control, 3: 40mg/ml, 4: 50mg/ml, 5: 60mg/ml, 6: 70mg/ml,: 80mg/ml, 7: control. MTT was added, 3 minutes after that incubated for 4 hours. DMSO solution was added and then shaken, the samples were analyzed using ElISA reader with a 620 wavelength. The cytotoxiciy wa expressed by cell viability. If its is > 50%, it is declared as non toxic. Data analyzed using non parametric test (Kruskal-Wallis) followed by Mann Whitney test. Result: The Kruskal-Wallis test proved that there were significant differences in the cell viability among the treated groups. The average difference among the treated groups which were tested with Mann-Whitney test, showed a significant difference between group 1 and 5, also group 2 and group 5. Conclusions: Acanthus ilicifolius leaf chloroform extract has no cytotoxicity effect on concentration fibroblast cell (BHK-21) culture.Keywords: Acanthus ilicifolius, root canal irrigation, fibroblast cellCorrespondence: Twi Agnita Cevanti, Laboratorium Konservasi Fakultas Kedokteran Gigi Universitas Hang Tuah, Jl, Arif Rahman Hakim 150, Surabaya, Indonesia. Ph 031-5945864, fax: 031-5912191, e-mail address: [email protected]
Sularsih Sularsih, Michelle Suhartono, Nafi’Ah Nafi’Ah
Published: 1 February 2018
DENTA, Volume 12; doi:10.30649/denta.v12i1.156

Abstract:
Background: Traumatic ulcer is one of the most common oral wounds. Chitosan has mucoadhesive characteristic while Aloe vera containing lignin which is able to penetrate the skin. It is expected that the combined gel of chitosan and Aloe vera will function as wound healing accelerator in traumatic ulcer. Molecular weight is one of the characteristics of chitosan quality. Purpose: the aim of this experiment was to know the density of collagen fibers in wound healing of traumatic ulcer using the combined gel of chitosan with different molecular weight and Aloe vera. Materials and method: 30 Male Rattus Norvegicus were divided into 3 groups. Group I was control group (without chitosan and Aloe vera), group II was given low molecular weight chitosan and Aloe vera, group III was given high molecular weight chitosan and Aloe vera. The groups were given traumatic ulcer making with 4 mm diameter and 2 mm depth. Rats were sacrificed by decapitation on day 3 and 7 then they were examined histopatologically to see the density of collagen fibers. Result: Statistical analysis with Kruskall Wallis and Mann-Whitney U test showed that there were significant difference p<0,05 between high and low molecular weight chitosan with Aloe vera group on day 3 and 7. Conclusion: Chitosan with high molecular weight and Aloe vera were more effective in wound healing of traumatic ulcer because they increase the density of collagen fibers. Keywords: Combined gel of chitosan and Aloe vera, density of collagen fibers, wound healing. Korespondensi: Sularsih, Bagian Ilmu Biomaterial Kedokteran Gigi, Fakultas Kedokteran Gigi, Universitas Hang Tuah, Arif Rahman Hakim 150, Telepon 031-5912191.
, Aulia Dwi Maharani
Published: 1 February 2018
DENTA, Volume 12; doi:10.30649/denta.v12i1.158

Abstract:
Background: The company is able to be the basis of the formation of customer loyalty and fanaticism of the product or service through a strong brand. Having a strong brand is a vital aspect for any company, because the advantage gained diverse. Based on the data in 2014 and 2015, it showed the decreased number of patients at the Nala Husada Dental Hospital from 2014 to 2015 amounted to 949 people (8%), as well as the number of patients in the Polyclinic of Dental Specialists, were 14,6% (348 patients) from ideal capacity (2384 patients). Purpose: The aim of this study is to find out the influence of brand attributes to brand awareness in Nala Husada Dental Hospital. Methods: This study was an observational study that used 200 respondences from the patients population of Nala Husada Dental Hospital. The study was conducted at Nala Husada Dental Hospital from March-April 2018. Results: The results showed that the characteristics of patients who seek treatment at the Nala Husada Dental Hospital were majority adults in productive age (25-35 years old), at middle to lower-end of socio-economic class, and had the education level of SLTA grade. Most of respondents had bad perception for the brand attributes of Nala Husada Dental Hospital. Most of them had low brand awareness towards the Nala Husada Dental Hospital. There was a tendency if the brand attributes were good, the brand awareness was getting better.Keywords: Brand Attributes, Brand Awareness.Correspondence: Ghita Hadi Hollanda; Department of Dental Public Health; Faculty of Dentistry; Universitas Hang Tuah; Arif Rahman Hakim No. 150, Surabaya; Phone +6231-5912191; Email: [email protected]
, Annisa Rahmawati, Isidora Karsini S
Published: 1 February 2018
DENTA, Volume 12; doi:10.30649/denta.v12i1.163

Abstract:
Background: The Sarggasum’s sp. extract contains of several chemical properties such as flavonoid, vitamin A, vitamin C, zinc, and calcium which has benefit as anti-inflammation, so it can be used as an alternative therapy for traumatic ulcer. Purpose: To investigate the effectivity of Sargassum’s sp. extract towards the amount of macrophages in the healing process of traumatic ulcers. Material and Method: 32 wistar rats were divided into 4 groups, K1 the negative control group without treatment, K2 the positive control group with the drugs containing 0.2% hyaluronic acid, and the treatment group K3 Sargassum’s sp. extract concentrations of 50% and K4 Sargassum’s sp. extract concentrations of 75%. Traumatic ulcer were made in the labial mucosa of rats by using heated amalgam stopper. On the fourth and seventh day, rats were sacrified and biopsied on lower labial mucosa. The macrophages were observed using a 1000x magnification light microscope with OptiLab viewer. Result: The statistical test using One Way ANOVA showed significant difference (p<0,05) of macrophage on fourth day group. The LSD test showed a significant difference (p<0,05) in macrophages at K1 (2.83±1.03) compared to K3 (1.17±0.64), K1 compared to K4 (0.58±0.79), K2 (2.58±0.50) compared to K3, and K2 compared to K4 at the fourth day group, whereas the seventh day group showed no significant differences between each groups. Conclusion: The Sargassum’s sp. extract concentrations of 75% is the most effective in decreasing amount of macrophages in the healing process of traumatic ulcers. Keywords: traumatic ulcer, inflammation, macrophage, Sargassum sp. extract Correspondence: Agni Febrina Pargaputri, Department of Oral Biology, Faculty of Dentistry, Hang Tuah University, Arief Rahman Hakim 150, Surabaya, Phone. 031-5912191, Email: [email protected]
Arya Barahmanta, Muhammad Faizal Winaris, Pambudi Raharjo
Published: 1 February 2018
DENTA, Volume 12; doi:10.30649/denta.v12i1.162

Abstract:
Background: Orthodontic tooth movement is a interaction prosess of resorption and deposition of bone remodeling. Orthodontic tooth movement by mechanical strength causes changes in alveolar bone. Osteocyte is an essential cell to respond bone remodelling. Hyperbaric Oxygen Therapy affects production of osteocyte because it can release Reactive Oxygen Species (ROS) and Nitrid Oxide (NO). Purpose: To determine the difference number of osteocyte in pressure and tension area during tooth movement by adjuvant of Hyperbaric Oxygen 2,4 ATA during 7 days starting on day 8 to day 14. Materials and Methods: This research used Completery Randomized Control Group Post Test Only Design. 36 cavia cobaya (male) were divided into 3 groups randomly : the negative control groups, positive control group, and treatment group. Preparat staining used Hematoxylin Eosin (HE) and calculated on microscop 1000x with 20 field of view. Data analyses used one way ANOVA and LSD test then compared each area by using paired T test. Result: The data showed that the treatment group (P=10,67) tension area has the highest number of osteocyte than negative control group (K-=3,67), positive control (K+=7,42). In the pressure area showed that negative control group (K-=5,00) has the highest than positive control group (K+=3,83) and treatment (P=3,25). Conclusion: Therapy HBO 2,4 ATA 7 days starting on day 8 to day 14 is could increase osteocyte in the tissue to stimulate process of bone remodelling. Keywords: Hyperbaric Oxygen, Tooth movement, Bone remodeling, Osteocyte Correspondence: Arya Brahmanta, Department of Orthodonty, Faculty of Dentistry, Hang Tuah University, Arif Rahman Hakim 150, Surabaya, Phone 031-5945864, Email: [email protected]
, Annete Juwita, Puguh Bayu Prabowo
Published: 1 February 2018
DENTA, Volume 12; doi:10.30649/denta.v12i1.167

Abstract:
Background: Self-cured acrylic resin is a material used for repairing broken and fractured dentures. Zirconium dioxide (ZrO2) is a reinforcing material that is being developed as an additive to the acrylic resin. Objective: to determine the effect of zirconium dioxide (ZrO2) addition on self-cured acrylic resin on impact strength. Material and Methods: This study was a true experimental laboratory study with the design of a post-test only control group design, using 24 pieces of self-cured acrylic resin measuring 65 x 10 x 2.5 mm divided into 4 groups with and without zirconium addition dioxide (ZrO2) nanoparticles. Zirconium dioxide (ZrO2) nanoparticles were silanized first by mixing the silane coupling agent. Furthermore, the impact strength was tested on the plate. The data obtained were analyzed using the One Way ANOVA test. Results: there were significant differences in the impact strength between the groups with a significance value of 0,000 (p <0.05). Conclusion: there was a difference in the increase of impact strength in acrylic resin self-cured with the addition of ZrO2 nanoparticles concentrations of 3%, 5%, and 7%.Key words: Self cured acrylic resin, zirconium dioxide, impact strength Correspondence : Widaningsih, Department of Prosthodonti, Faculty of Dentistry, Hang Tuah University, Arif Rahman Hakim 150, Sukolilo, Surabaya, Phone 0818312757, Email: [email protected]
Ayu Listya, Yulie Emilda Akhwan
Published: 1 February 2018
DENTA, Volume 12; doi:10.30649/denta.v12i1.165

Abstract:
Background : Untreated caries will continue and microorganisms enter the pulp resulting in an inflammatory response and pulp necrosis. Sterilization is one part of root canal treatment and is important for the success of root canal treatment. Stolephorus insularis contains antibacterial substances such as fluor. Previous research showed that extract Stolephorus insularis has antibacterial activity in Streptococcus mutans bacteria.Purpose : This study aims to determine the antibacterial power of Stolephorus insularis extract on the growth of Staphylococcus aereus bacteria by using 3 concentrations of 18%, 24% and 30%.Material and Method :This resesarch use culture of Staphylococcus aereus bacteria in MH blood agar, Stolephorus insularis extract with various concentrations (18%, 24%, 30%), 7% Na CMC, 1% DMSO solution, agar BHI media. Culture of Staphylococcus aereus was taken from liquid BHI media, then rubbed on the entire surface of BHI media using a sterile cotton stick. The antibacterial zone formed is measured. Result : Based on the results of the Mann-Whitney test there were significant differences in the inhibitory power of Staphylococcus aureus bacteria (p <0.05) in the K + group with groups K-, P1, P2 and P3. Whereas between groups P1 with K-, group P2 with K- and P1, and group P3 with K-, P1 and P2 there were no significant differences because the significant value was greater than 0.05Conclusion : There is no antimicrobial power at concentrations of 18%, 24%, and 30%. Quantitative research needs to be done to determine the decrease in the number of bacterial colonies.Keywords : Inhibition effect, Stolephorus insularis, Staphylococcus aereusCorrespondence : Ayulistya Paramita, Paediatric Departemen. FKG UHT, Jl Arief Rahman Hakim no 150 Surabaya. email : [email protected]
Widaningsih Widaningsih, Ajeng Erlinda Muchtar, Anindita Apsari
Published: 1 February 2018
DENTA, Volume 12; doi:10.30649/denta.v12i1.166

Abstract:
Background: Heat cured acrylic resin is a material that has been used as a base for dentures. The main recruitment of ideal dentures include esthetic, durable, and also the how hard the surface itself. A dirty denture can lead to smell of the mouth, unesthetic, and inflammatory in the oral cavity. One of the material denture cleanser made of natural can inhibit the growth of fungal contained in Sargassum ilicifolium that have content of Mg, Na, Fe, and Fenol. Purpose: The aimed of this research is to understand the effect of soaking heat cured acrylic resin as the dentures material in 40% Sargassum ilicifolium towards the hardness surface. Methods: 20 plates of 65 x 10 x 2,5 mm heat cured acrylic divided into 4 different group as samples. First group soaked for 270 hours in aquades, second group soaked for 630 hours in aquades, third group soaked for 270 hours in 40% Sargassum ilicifolium extract, and the fourth group soaked for 630 hours in 40% Sargassum ilicifolium extract. The time of the research 270 hours equivalent of the use of the denture for 3 years, 630 hours equivalent of the use of the denture for 7 years. Then, researcher tested the hardness of all four group surface using Vickers Hardness Tester by using 0,20 kg indenter weight. Result: The result of Kruskal Wallis parametric description showed that there is a difference number of the surface hardness between Aquades and Sargassum ilicifolium 40%. Mann Whitney test result also showed that there are significant number each groups Aquades and Sargassum ilicifolium. Conclusion: A number of the hardness surface of heat cured acrylic resin were soaked using 40% Sargassum ilicifolium extract for 630 hours is lower than compared heat cured acrylic resin were soaked using 40% Sargassum ilicifolium extract for 270 hours. Key words: Acrylic resin. Sargassum ilicifolium, Surface hardness, Vickers Hardness Tester Correspondence: Widaningsih, Department of Prostodontics, Faculty of Dentistry, Hang Tuah University, Arif Rahman Hakim 150, Surabaya, Phone 031-5945864, Email: [email protected]
Ghita Hadi Holanda
Published: 1 February 2017
DENTA, Volume 11; doi:10.30649/denta.v11i1.120

Abstract:
Background: The company is able to be the basis of the formation of customer loyalty and fanaticism of the product or service through a strong brand. Having a strong brand is a vital aspect for any company, because the advantage gained diverse. Based on the data in 2014 and 2015, it showed the decreased number of patients at the Dental Hospital of Hang Tuah University (UHT Dental Hospital) from 2014 to 2015 amounted to 949 people (8%), as well as the number of patients in the Polyclinic of Medical Specialists of UHT Dental, were 14,6% (348 patients) from ideal capacity (2384 patients). Purpose: The aim of this study is to find out the influence of branding to brand awareness in dental hospital of Hang Tuah University. Materials and Methods: This study was an observational study that used 200 respondences from the patients population of UHT Dental Hospital. The study was conducted at UHT Dental Hospital from March-April 2016. Result: The results showed that the characteristics of patients who seek treatment at the UHT Dental Hospital were majority adults in productive age (25-35 years old), at middle to lower-end of socio-economic class, and had the education level of SLTA grade. Most of respondents had bad perception for the brand attributes of UHT Dental Hospital. Most of them had low brand awareness. The majority of respondents rated the branding of UHT Dental Hospital were bad. Conclusion: There was a tendency if the branding were good, the brand awareness was getting better.Keywords: Branding, Brand AwarenessCorrespondence: Ghita Hadi Hollanda; Department of Dental Public Health, Faculty of Dentistry, Hang Tuah University, Arif Rahman Hakim 150, Surabaya; Phone 031-5912191, Email: [email protected]
Published: 1 February 2017
DENTA, Volume 11; doi:10.30649/denta.v11i1.134

Abstract:
Background: Periodontal tissue is a connective tissue that sorrounding the tooth and consists of gingiva, cementum, periodontal ligament and alveolar process. The periodontal disease affecting the soft tissue sorrounding the tooth without any alveolar loss, periodontal ligament or cementum and it is called as gingivitis. If it is untreatment as soon as possible then it will grow fast and infected the alveolar process resulting periodontitis. Gingival Recession is defined as the apical displacement of the gingival margin. Gingival recession showing first step in cemental damage, the exposure of the dentinal tubuli, causing pain and discomfort. Periodontal surgery is the continuing treatment in gingiva. Subpithelial connective tissue graft have been highly predictable in gingival resession therapy with respect to a high percentage of wound healing of the connective tissue.Keywords: gingival recession, subepithelial connective tissue graft, periodontal surgeryCorrespondence: Erianti Dewi Utami, Department of Periodontics, Faculty of Dentistry, Prof. Dr. Moestopo (B) University, Bintaro Permai Raya No.3, Pesanggrahan, Daerah Khusus Ibukota Jakarta, Phone (021) 73885254, 08129121278, Email: [email protected]
Yongki Hadinata W, Karlina Samadi
Published: 1 February 2017
DENTA, Volume 11; doi:10.30649/denta.v11i1.133

Abstract:
Background : There are some factors can cause endodontic failure such as inadequate in cleaning or shaping step, non hermetic obturation, or poor restoration, which can cause bacteria multiply. Purpose : To report the management of endodontic failure with nonsurgical treatment. Case : 46-year-old woman came to Airlangga Dental Hospital Conservative Dentistry Department to treat her upper right tooth which show symptomatic pain in the last 2 weeks. The tooth has been treated and crowned with porcelain fused to metal about 10 years ago. Clinical examination show the presence of fistula on premolar buccal gingiva, react to percussion. Radiographic examination show not hermetic obturation in one root canal and radiolucency in the periapical area. The diagnosis for maxillary first premolar is previously treated tooth with chronic periapical abscess.. Treatment : Crown and post was removed from the tooth, and endodontic retreatment was done. Follow up 6 months after the retreatment show no reaction to percussion, and radiographic examination show no enlargement periapical lesion. Conclusion : Nonsurgical endodontic retreatment always become the first choice to resolve endodontic failure for previously treated tooth.Keywords : endodontic failure, maxillary first premolar, nonsurgical endodontic retreatmentCorrespondence: Yongki Hadinata W., drg. PPDGS Ilmu Konservasi Gigi Fakultas Kedokteran Gigi Universitas Airlangga, Surabaya. Jl. Mayjen. Prof. Dr. Moestopo No. 47, Surabaya.
Chaterina Diyah Nanik K, Anindita Apsari
Published: 1 February 2017
DENTA, Volume 11; doi:10.30649/denta.v11i1.132

Abstract:
Background: One of the most common inflammation disease in the oral cavity for the past few years is the aggressive form of periodontitis. Common signs of aggressive periodontitis is the mobility of teeth especially in incisive and first molars, and occuring mostly in young patient. Young patients whose losing her anterior teeth, are a real challenge for dentist and prosthodontist. Prosthodontist need to consider both functional and esthetic aspects. Objective: Rehabilitation of young adult patient with aggressive periodontitis by an interdisciplinary approach of orthodontist and prosthodontist. Case Description: A young woman, suffered from aggressive periodontitis with major complain of her teeth mobility, especially incisive and first molar in mandible. She had undergone periodontal treatment, but the result was failed. The anterior teeth in mandible need to be extracted, therefore patient wished not to be in edentulous state. As preliminary treatment, we choose immediate denture to replace the anterior mandible teeth. We faced difficulties in mandible, because her right canine weren’t in the proper dental arch. So we asked orthodontist to place fixed orthodontics in mandible, to get the canine back in the proper arch. We’ve chosen orthodontic treatment,because we didn’t want to extract the canine teeth. We evaluated in six months and after the canine back in the proper arch, we proceed to long span bridge in mandible as our definitive treatment. Conclusion: By not extracting the canine teeth, we got some advantages, especially patient psychically was happier with her own teeth. The collaboration with another dentistry field, provides us better treatment for patient. After treatment, patient had no complaints and was happy with her new smile.Keywords: Prosthodontic rehabilitation, aggressive periodontitis, orthodontic treatment, esthetic.Correspondence: Chaterina Diyah Nanik. K; Department of Prostodontitics, Faculty of Dentistry, Hang Tuah University, Arif Rahman Hakim 150, Surabaya; Phone 031-5912191, Email: [email protected]
Published: 1 February 2017
DENTA, Volume 11; doi:10.30649/denta.v11i1.130

Abstract:
Background: The uses of silver nanoparticle are growing so fast, especially in the medical science, it’s used in many kinds of concentration. In dentistry, it’s used to decrease halitosis and periodontal diseases, and also wound healing process. It can affect the viability of the cells, give the bad effects to the human’s health and environment if we use it in a long-term and in an uncertain concentration. Purpose: The aim of the study was to analysis the healing process of gingival fibroblast in Rattus novergicus after exposed by 15 µg/ml silver gel nanoparticles. Materials and Methods: This study uses 9 male wistar rats were divided into 3 groups. Group A: Cut (hurt it) in the oral gingiva and exposed to Ag-Np Gel 15 µg/ml for 3 days. After 3 days, they were sacrificed and cut the gingival fibroblast off 3x4 cm size with scalpel. Group B: cut in the oral gingiva and exposed to Ag-Np Gel 15 µg/ml for 5 days. After 5 days they were sacrificed and cut the gingival fibroblast off 3x4 cm size with scalpel. Group C: cut in the oral gingiva and exposed to none for 3 days then cut the gingival fibroblast off 3x4 cm size with scalpel. The expressions of IL-8 in the wound healing process were painted by Immunohistochemical method. This data was analyzed by using the t-test method. Results: General characteristic of IL-8 is shown in brown color immunoreactive expression on macrophage via immunohistochemical painting with monoclonal antibody anti IL-8. Mean expression numbers of IL-8 in the group A = 11,7; group B = 16,3; and group C (control) = 28,3. T-test sign.number of group A & C = 0,004; group B & C = 0,000. Conclusion: The exposure of 15 µg/ml silver gel nanoparticle to gingival fibroblast of Rattus novergicus reduces the expressions of IL-8 in the day-3 and day-5 post exposure. So, it may accelerate the wound healing process.Keywords: Healing, silver, nanoparticles, IL-8Correspondence: Kharinna Widowati, Department of Science Mouth Disease, Faculty of Dentistry, Hang Tuah University, Arif Rahman Hakim 150, Surabaya, Email: [email protected]
Dwi Hariyanto, Aprilia Aprilia, Annisa Mutiara Pertiwi
Published: 1 February 2017
DENTA, Volume 11; doi:10.30649/denta.v11i1.122

Abstract:
Background: Individuals in our contemporary society tend to depend on various types of services, including health care service. Managers of health care services must be aware of this situation to improve image and reputation as a public’s choice services. Image is an award obtained by a company for their excellence in services. In improving the image, a company needs the public’s or consumer’s appraisals for a marketing strategy formation. Purpose: As information to develop the image of Dental and Oral Hospital of Hang Tuah University, Surabaya. Materials and Methods: descriptive analysis with cross sectional research design was used in this study. Simple random sampling of probability sampling technique was used to choose 100 respondents of this study. Respondents were given a questionnaire to assess the image of Dental and Oral Hospital, Hang Tuah University Surabaya. The results of the questionnaire were analyzed by using descriptive statistics. Result: Results on the image of Dental and Oral Hospital of Hang Tuah University showed that 83 respondents rated moderately good image, while 17 respondents rated poor image. Conclusion: Based on the SWOT matrix, it can be concluded that several development through consideration of internal and external factors can be beneficial to the progress of Dental and Oral Hospital of Hang Tuah University, Surabaya. Keywords: Image, Marketing Strategy, Consumer Behavior, SWOT Analysis Correspondence: Dwi Hariyanto, Public Dentistry, Faculty of Dentistry, Hang Tuah University, Arief Rahman Hakim 150, Surabaya, Phone 031-5912191. Email: [email protected]
, Robbi Akbar Dirmadana, Ghina Sucilia Mediani, I Ketut Ika Sandana, Febryan Alief, Jessica Jenuary Yasin
Published: 1 February 2017
DENTA, Volume 11; doi:10.30649/denta.v11i1.121

Abstract:
Background: The prevalence of malloclussion in Indonesia reached 80% in 2013, accordingly, orthodontic treatment needs also increased. Orthodontic treatment is a treatment to improve the arrangement of teeth. Orthodontic treatment causes movement of the teeth, but this treatment takes quite long time around 15-24 months and it needs an additional therapy to accelerate it.. Fibroblast is a main cell in periodontal ligaments that has an important role on orthodontic tooth movement. Stichopus hermanii (golden sea cucumber) and Hyperbaric Oxygen Therapy (HBOT) can induce fibroblast proliferation. Purpose: To investigate how the innovation of Stichopus hermanii and HBOT can increase the number of Fibroblast in periodontal ligament during orthodontic tooth movement. Materials and Methods: This research used Randomized Post Test Only Control Group Design. The sample involve 30 male adult guinea pigs was randomly divided into 5 groups: negative groups (K-), positive groups (K+), Stichopus hermanii treatment group (P1), HBOT group (P2), and combination group(P3). In K+ and treatment groups, maxillary incisors of guinea pig was placed a helical spring in order to move the teeth to distal. To analyze the number of fibroblast on histology, used Hematoxylin Eosin (HE). Data analysis uses One Way ANOVA, Post Hoc LSD, and Paired T Test. Result: Research data shows an increased average of fibroblast number in the pressure area, K(-): 10,8±0,83; K(+): 9±1,14; P1: 10±1,58; P2: 10,6±1,51; P3: 11±1,41 and in the tension area also experienced an increased average of fibroblast number, K(-): 11,6±1,11; K(+): 12,6±2,88; P1: 14,6±1,14; P2: 16,6±1,14; P3: 18,2±1,92. Conclusion: Innovation of Stichopus hermanii and HBOT increased the number of fibroblast in periodontal ligament during orthodontic tooth movement. Keywords: Orthodontic tooth movement, Stichopus hermanii, HBOT, fibroblast, periodontal ligament. Correspondence: Arya Brahmanta, Department of Orthodonti, Faculty of Dentistry, Hang Tuah University, Arif Rahman Hakim 150, Sukolilo, Surabaya, Phone 081233660077, Email: [email protected]
, Elly Munadziroh, Retno Indrawati
Published: 1 February 2017
DENTA, Volume 11; doi:10.30649/denta.v11i1.125

Abstract:
Background: Enterococcus faecalis and Fusobacterium nucleatum are the most common bacteria found in infected root canal teeth and most of them often caused failure in endodontic treatments. These bacteria can form biofilm which makes them more resistant against antibacterial agents. Biofilm formation also causes a decrease in antibiotics and antimicrobials sensitivity. Pluchea indica Less leaves is a species of plants that has several chemical properties. It consists of flavonoids and polyphenols which have benefits to inhibit biofilm formation. Because of its benefits, the extract of Pluchea indica Less leaves can be potentially developed as one of sterilization dressing in root canal teeth. Purpose: The aim of this study was to determine biofilm formation inhibition of Pluchea indica Less leaves extract against Enterococcus faecalis and Fusobacterium nucleatum. Materials and Methods: The dilution method was done first to show the Minimum Inhibitory Concentration (MIC) of the extract. The inhibition biolfilm formation was tested using microtitter plate assay by measuring the bacterial biofilm Optical Density (OD) from ELISA reader’s results and using autoagregation assay to show the inhibition of adherance bacteria. The Pluchea indica Less leaves extract concentration used for inhibition of biofilm formation were 100%, 50%, 25%, 12,5%, and 6,25%. Results: The result of biofilm formation inhibition showed that Pluchea indica Less leaves extract were able to inhibit Enterococcus faecalis and Fusobacterium nucleatum’ biofilm formation with strong moderate effect. The autoagregation assay showed a decrease in autoagregation percentation of Enterococcus faecalis and Fusobacterium nucleatum. Conclusions: Pluchea indica Less leaves extract has effect to inhibit biofilm formation of Enterococcus faecalis and Fusobacterium nucleatum.Keywords: Pluchea indica Less leaves extract, Enterococcus faecalis, Fusobacterium nucleatum, biofilm.Correspondence: Agni Febrina Pargaputri, Department of Oral Biology, Faculty of Dentistry, Hang Tuah University, Arif Rahman Hakim 150, Surabaya, Phone.031-5912191, Email: [email protected]
, Endah Wahjuningsih
Published: 1 February 2017
DENTA, Volume 11; doi:10.30649/denta.v11i1.124

Abstract:
Background: The traumatic ulcer is the most common oral soft tissue lesion that cause physiologic dissorder (pain) in patients, so it need a medicine to improving healing processs.Stichopus hermanii contains glycosaminoglycans, omega-3 and other agents that accelerate wound healing. Their influence againts signaling cell for proliferation and angiogenic for healing procce. Endoglin plays in a number of cellu- lar processes and the elevated expression during wound healing. Purpose: Knowing the effectivity of ethanol extract of Stichopus hermanii towards the expression on traumatic ulcer maturation phase in oral mucosa. Materials and Methods: The research subjects were forty eight wistar rats divided into 6 groups. Traumatic ulcer was made in the center-rat’s-labial-mucosa. The X1 group was smeared with sterilized aquades as the control group. The X2 wassmeared with 0,2% hialuronic acid, X3 with 60% ethanol extract, and X4 with 80% ethanol extract. They were smeared once a day during 4 days. On the sixth day, the rat’s labial mucosa was biopsied to make histological preparation in order to study the amount of fibroblast. Results: Kruskal-Wallis test showed not significant difference (p=0,000) after applying Stichopus hermanii extract for expression of endoglin in Conclusions: ethanol extractStichpus hermanii not effective for expression of endoglin on traumatic ulcer maturation phase in healing procces. Keywords: Stichopus hermanii, ulcer healing, endoglin and traumatic ulcer. Correspondence: Rima Parwati Sari, Bagian Biologi Oral, Fakultas Kedokteran Gigi Universitas Hang Tuah, Arif Rahman Hakim No. 150, Surabaya, Email: [email protected]
, Chaterina Dyah Nanik K
Published: 1 February 2017
DENTA, Volume 11; doi:10.30649/denta.v11i1.129

Abstract:
Background: Telescopic double crown restoration can be used as an alternative treatment if the patient wants the tooth to be maintained as long as the supporting tooth has a healthy periodontal. In dental preparation telescopic double crown support requires a preparation guide so that the preparation can be acurate. Minimal preparation can cause the results of the telescopic double crown restoration to be overcontoured, so that the patient may occur uncomfortable, speech disorders and esthetic is not good. Objective: to provide information about the importance teeth preparation guidelines for prosthodontic treatment with telescopic double crown restorations. Case: A 72-year-old female patient, coming to the dentist wants to make dentures so that she can chew food well and improve her appearance. The remaining teeth are only 13 and 23. In making the denture, the patient wants the teeth to be retained (not removed) and the denture is aesthetically good. Case Management: Make a diagnostic wax-up on a semiadjustable articulator to estimate the results of restoration, mould the patient's teeth before starting the preparation with three times putty for the preparation guide and making a temporary crown. the first putty mold is cut mesial-distal right in the middle of the incical teeth, the second putty mould is cut in the labial-palatal direction right in the middle of the tooth mould, the third putty mould is fixed on the stock tray to make the temporary crown. Preparation of teeth 13 and 23 with regard to the putty cut preparation guide, then moulding the maxilla and mandibula with double impressions using an individual tray, after that make the temporary crown directly with the third putty mould. Conclusion: The preparation guide of teeth is very necessary so that the making of telescopic double crown restorations is not overcontoured, so that esthetic and retention can be achieved which are good and do not cause speech disorders.Keywords: Telescopic double crown, tooth preparation guide, estheticCorrespondence: Anindita Apsari. Prostodonsia, Fakultas Kedokteran Gigi, Universitas Hang Tuah, Arief Rahman Hakim 150, Surabaya, Telepon 031-5945864, 5912191, Email: [email protected]
Budi Handayani, Lisdiana Mardanus
Published: 1 February 2017
DENTA, Volume 11; doi:10.30649/denta.v11i1.126

Abstract:
Background: Orthodontic treatment requires time so, duration of orthodontic treatment time needs to be shortened in various ways. Flavonoid is an active content of propolis, that might accelerate tissue regeneration. Flavonoid might increase the number of fibroblast at tension side of orthodontic tooth movement. Purpose: The purpose of this study was to examine the effect of propolis extract gel towards the number of fibroblasts at tension side to accelerate orthodontic tooth movement. Materials and Methods: Thirty male Cavia cobayas were divided into three groups. The first group, ( P) was given treatment to make orthodontic tooth movement and propolis extract gel. The second group, K(+) was given treatment and NaCMC gel. The third group, K(-) was served as control. After 14 days, the Cavia cobayas were sacrified. Fibroblast number was examined with light microscope 400 X. Result: Mean and SD in K(-):100 ± 13,2; K(+):80 ± 13,05; (P):176 ± 17,9. The result of ANOVA test showed significant increase in the number of fibroblasts. The LSD test showed significant result in all groups with significance result p ≤ 0,05. Conclusion: Propolis extract can increase fibroblast number to accelerate orthodontic tooth movement. Keywords: Orthodontic tooth movement, propolis extract, Cavia cobaya, fibroblast. Correspondence: Departement of Orthodontics, Faculty of Dentistry, Hang Tuah University, Arif Rahman Hakim 150, Surabaya, Phone 031-594864, Fax 031-5912191, Email: [email protected]
Benita Aryani Hendrata, Paulus Budi Teguh
Published: 1 February 2016
DENTA, Volume 10, pp 100-108; doi:10.30649/denta.v10i1.42

Abstract:
Background: An anterior tooth extraction can cause aesthetic and psychological problems. An Immediate Removable Partial Denture could be the choice of aesthetic rehabilitation because is inserted immediately following the natural tooh extraction. Purpose: This paper reported the case of Immediate Removable Partial Denture for aesthetic anterior maxillary tooth rehabilitation in a 41-years old woman teacher. Case Report: A 41-years-old woman teacher wasn’t confident with her #22 tooth microdontia and malpositioned. In order to rehabilitated her aesthetic problem could be done by immediate #22 tooth extraction. Case Management: Removable Partial Denture preparation was done by removing the #22 tooth. The denture was inserted immediately after the extraction. After the denture was inserted, at the 24-hours, 3 days, 1 week, and 1 month should be evaluated to know the bone resorption process and whether the Immediate Removable Partial Denture should be relined or not. Conclusion(s): The Immediate Removable Partial Denture provides an aesthetic rehabilitation and increases the patient’s self-confidence because the patient has no edentulous period.
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