Journal Jurnal Iktiologi Indonesia-
Jurnal Iktiologi Indonesia, Volume 19; doi:10.32491/jii.v19i2.479
Abstract:Rainbow fish (Iriatherina werneri) is a type of partial spawner fish with the little number of eggs production. The high contain of essential fatty acid (Linoleic acid 18:2 Ω-6) in corn oil was widely used as a source of fatty acid in the broodstock feed. The aim of this study was to evaluate the additional of LA fatty acid in feed for reproduction performance of rainbow fish. This study used a complete random design consists of three treatments and six replicates with different doses of corn oil LA in feed, Ω-6 0%, Ω-6 1%, and Ω-6 2%. Rainbow fish broodstock as sampel test with body weight 0.10-0.18g for females 0.21 ± 0.001 g, were kept separately in each tanks (size 30 cm x 30 cm x 30 cm) with density 15 fish per tanks. Broodstock were given diet three times daily at 08:00 am, 12:00 am and 17:00 pm by at satiation. The trial feed was administrated for 30 days before conducted the mass spawning for seven days with ratio of the male and female broodstock was 1:3. The results showed that treatment without supplementation of Ω-6 corn oil performed highest number of eggs 290 ± 125, hatching rate 55,99 ± 14,80%, survival rate 8,43% and the proportion of vitellogenic cell was more dominant, but the best of larval performance was observed in tretament of Ω-6 1% of highest larval length 3.00 mm and 100% percentage of normal larvae. It can be concluded that the supplementation of 1 % the Ω-6 fatty acid corn oil in the feed to produce reproductive performance was supported by the results of the histology of the eggs and larvae of the best performance in rainbowfish.
Jurnal Iktiologi Indonesia, Volume 19; doi:10.32491/jii.v19i2.480
Abstract:Caroun croaker (Johnius carouna) is one of demersal fishes which are commonly caught in the south of Java waters. This fish captured by trammel net, gillnet, danish seine and mini trawl. This study aims to determine length and weight relationship, growth parameter, length frequency distribution and condition factors of croaker fish caught in the south of Java waters. This study was conducted from January-December 2015 at the Cilacap Ocean Fisheries Port, Central Java. Data collected was total length (TL in mm) and weight (W in g) of caroun croacker fish. A total of 2141 of Caroun croaker was caught by mini trawl during the sampling periods. The result showed that the size of croaker fish caught by mini trawl ranged between 85-225 mm with an average was 143.7 mm. Length and weight relationship expressed by equation of W = 0.0062 TL3.2889 ( R2 = 0.9443). The growth pattern of croaker fish was allometric positive. The Von Bertalanffy growth equation of caroun croacker fish in south of Java waters was Lt =155 (1 - e -0.9(t+0.2127)) with the asymptotic length (L∞) =155 mm, growth coefficient (K) = 0.9 per year and the theoretical age croaker fish that was equal to (t0) = - 0,2127.
Jurnal Iktiologi Indonesia, Volume 19; doi:10.32491/jii.v19i2.482
Abstract:Javanese long pepper (Piper retrofractum) is known to have androgenic effect, could act as phytosteroid and alternative to masculinization. This research aimed to evaluate the optimum dose of the Javanese long pepper extracts combined with increased rearing temperature on featherfin squeaker masculinization through larva immersion. This research was designed using a completely randomized design with 8 treatments consisted of Javanese long pepper extract doses of 0.0625 mg L-1and 0.125 mg L-1, negative control (without both extract and 17α-metiltestosterone) and positive control (2 mg L-117α-metiltestosterone). Each of them consisted of two different temperature treatments, namely, normal (26-27ᴼC) and 32ᴼC. After five hours treatment, larvae were reared until five months age. The fish age four and then five months were dissected and their gonads were taken for acetocarmine and histology preparation. Beside that the percentage of fish survival, body length, weight gain, and specific growth rate were also recorded in certain period. The result showed that the Javanese long pepper extract dose at 0.125 mg L-1 with normal temperature treatment produced the highest male ratio and significantly different compare with control. Giving this dose to larvae did not affect fish survival and growth, meanwhile increased temperature treatment could not increase the effectivity of Javanese long pepper extract.
Jurnal Iktiologi Indonesia, Volume 19; doi:10.32491/jii.v19i2.478
Abstract:Vaccination is one of the most effective control measure in preventing Streptococcus agalactiae infection. In this study, vaccine coatings were prepared with certain biomaterials such as chitosan, skim milk, and maltodextrin at concentration 1 % or 10 %, and further freeze-dried. Vaccine selected (in vitro) by tests: viability cell, solubility, protein concentration and protein molecular weight. The result of in vitro test showed that chitosan coating at doses 1 % and 10 % were the best in solubility and protein concentration test. Vaccine coated with chitosan was administrated again by injection (in vivo) in nile tilapia (Oreochromis niloticus) before challenged by S. agalactiae. This test consists of six treatments and three replications, i.e. the fish were injected with liquid vaccine (A), Phospat Buffered Saline (PBS) (B), vaccine with chitosan coating 1 % (C), vaccine with chitosan coating 10 % (D), chitosan 1 % (E), and chitosan 10 % (F). Parameters were observed including survival rate, relative percent survival (RPS), antibody titre, and total leucocyte. The results showed that treatment C showed a significant difference (P < 0,05) in survival rate 92.22 ± 3.85 %, RPS 85.21 ± 7.20 %, and antibody titre highest result, but not significant (P > 0,05) to increase total leukocyte in experimental fish against S. agalactiae infection. In conclusion, vaccine freeze dry with chitosan coated 1 % is effective to improve immunity system of nile tilapia.
Jurnal Iktiologi Indonesia, Volume 19; doi:10.32491/jii.v19i2.483
Abstract:The quality of feed can be improved by fermenting feedstuffs and supplementing with feed additives. This study aims to determine the effect of supplementation of Lumbricus sp. extract in fermented feed on growth performance, body chemical composition, and milkfish hepatosomatic index. Milkfish with an initial weight of 17.80 ± 0.20 g head-1, rearing with a density of 20 fish per hapa net measuring 1 m3 by 24 pieces. Hapa net is installed in a pond with a water level of ± 60 cm. Research was design in completely randomized design with two factors. The first-factor was supplementation method, namely Lumbricus sp. which sprayed on feed and mixed with feed ingredients. The second factor was the dose of Lumbricus sp. extract, namely 0, 100, 200 and 300 mL kg-1 of feedstuffs. The fish was reared for 50 days and fed with 5% of fish biomass per day with feeding frequency of 3 times i.e., morning, afternoon and evening. The results showed that the supplementation Lumbricus sp. extract has no significant effect on all parameters (P>0,05). However, the level dose of Lumbricus sp. extract supplementation in feed has a significantly effect (P0,05) on survival and chemical composition of milkfish body. The best best of absolute growth (16.94±4.0 g), relative growth (48.71±5.77%), feed efficiency (40.74±10.3 %), and hepatosomatic index (1.5 ± 0.17) were found in the experiment of supplementation Lumbricus sp. extract mixed with feedstuffs at a dose of 300 mL kg1. The survival rate of milkfish was ranging from 68.33±29.3 to 91.,33±7.64%, while the body's chemical composition including protein levels, fat, ash, crude fiber, NFE / Nitrogen Free Extract, liver glycogen and muscle glycogen were 69.45±1.23 – 71.45±0.97%, 14.86±0.46 – 17.24±0.76%, 9.28±0.12 – 11.12±0.46%, 1.54±0.09 – 1.66 ± 0.13%, 1.33± 0.42 – 2.71±0.21%, 7,11±0,08 – 7,40±0,17mg g-1 and 6,13±0,44 – 6,45±0,40 mg g-1, respectively.
Jurnal Iktiologi Indonesia, Volume 19; doi:10.32491/jii.v19i2.476
Abstract:In order to the success of domestication programs of Asian redtail catfish is necessary to evaluate the level of change in each generation produced. This study aimed to evaluate the performance of growth and survival rate on three populations from each generation spawned of Asian redtail catfish (G-1, G-2 and G-3). The study was conducted experimentally used completely randomized design (CRD) with 3 treatments and 3 replications from May to July 2017. The origin of the post larvae of Asian redtail catfish was the modified seeds from mass spawning of Asian redtail catfish of Cirata population. G-1 was the population resulted from the spawning of the oldest broodstock, G-2 was a population resulted from spawning of the first generation broodstock and G-3 was the resulted from spawning of the second generation broodstock. Spawning process was conducted in the same time. The age of the seeds was one week old after hatching with total length of 0.8-1.0 cm and body weight of 0.0082 ± 0.0001 g. The Asian redtail catfish seeds were reared in the aquarium with dimension of 70 cm 50 cm x 40 cm and equipped with recirculation system. Each aquarium was stocked seed with a density of 2,000 individual 100 L-1 of water. During rearing process (15 days), fish were fed at satiation with frequency of three times per day. Feed was adjusted to mouth opening of larvae. Initially, larvae on 1 to 7 days of rearing process were fed with artemia and after that fed with tubifex. The result showed that the highest growth and survival rate performance (P
Jurnal Iktiologi Indonesia, Volume 19; doi:10.32491/jii.v19i2.477
Abstract:Provision of local snakehead fish supply (Channa sp.) is come from nature. If demand for local snakehead fish increases then the exploitation rate of this fish in nature will be excessive. It will cause the fish population in nature to decline. Domestication is an effort to prevent the delining of fish population. The aim of this study was to ensure that snakehead from the Arongan Lambalek Area, West Aceh Regency can survive, grow and develop in various cultivation containers, therefore can be cultivated by local communities. This study used an experimental method with a completely randomized design with three treatments of cultivation containers and three replications. The treatment was an aquarium container (P1), fiber container (P2), and tarpaulin container (P3). The size of snakehead fish used in this study had an average length of 2.72 cm and weight of 0.17 g. The results showed that the survival rate and the highest weighting value were found in the aquarium container treatment (P1), while the growth of fish length was found in the fiber (P2) . This study showed that domestication of the local snakehead fish in Arongan Lambalek, West Aceh was successfully carried out and the aquarium is the best container for rearing the domesticated local snakehead fish larvae.
Jurnal Iktiologi Indonesia, Volume 19; doi:10.32491/jii.v19i2.462
Abstract:The purpose of this study was to understand the morphometric and the meristic characters of the kelabau fish from the Siak, Kampar, and Rokan river populations that could be used as an initial step forconservation. A total of 113 kelabau from Kampar River, 58 fish from the Siak River and84 fishfrom the Rokan River was used in this study. Measurement of 21 morphological characters was conducted using a morphometric truss method. The analysis was carried out using a one-way ANOVA and Discriminant Function Analysis ( SPSS software). Meristiccharacteristics were analyzed descriptively for 30 fish per population. Results indicates that among 21 morphometrical characteristics, 19 characters shown a significant different (P 0.05). This result indicates that fish population from all sampling areas shown similar characteristics. The discriminant analysis results show that the specific characters of the O. melanopleurus were A5, A2, A6, B1and D4. Scatterplot results indicate that the Rokan River population was separate from the Siak and Kampar River populations, and the morphology of the fish from the Rokan River population was slightly different from those of the Siak and Kampar River population. The meristic characteristics of fish obtained from all sampling areas were relatively similar.
Jurnal Iktiologi Indonesia, Volume 19; doi:10.32491/jii.v19i2.481
Abstract:This study aimed to evaluate the growth of stunted elver Anguilla bicolor from three different periods of stunting process. Prior to experiment, the stunting process of elver was carried out by limiting the feeding rate at 2% of the fish biomass and temperature media with 24 oC for 2, 4, and 6 months period. The study used a completely randomized design with three different sources of stunted elvers (i.e., A = 2 months; B = 4 months; C = 6 months) as treatments with three replications. Measurement of RNA:DNA ratio, protein content, profile blood description and elver otolith growth was carried out at the beginning and end of rearing process. Post-stunting elvers were reared on artificial media designed according to elver habitat and controlled. Elver was stocked with 15 individuals in the artificial semi-natural media with a temperature of 28 oC and fed with 1 kg media-1 day-1 tubifex worm for three months. The results showed that the compensatory growth of stunted elvers increased two to three times with 100% of survival rate after three months of rearing process. Stunting has a significant effect on treatment B, indicated by the value of weight specific growth rates, RNA : DNA ratio, protein content, blood glucose levels and otolith growth of elvers. This study revealed that the growth performance of stunted elver reared in semi-natural media for four months is better than other treatments.
Jurnal Iktiologi Indonesia, Volume 19; doi:10.32491/jii.v19i1.446
Abstract:Indonesia is one of the biggest archipelago country in the world, These islands lined up, stretching from Sabang to Merauke. Some government institutions studied the number of islands in Indonesia. In March 2nd 2017 President of Republic Indonesia issued Keppres No. 6 2017 about 111 numbers of outer islands. Two of the islands belongs to Bengkulu Province, i.e Enggano and Mega islands. Stretching in an east-west direction embracing nearly 18,000 islands, the Indonesian Archipelago features a seemingly endless array of marine habitats. The country is the global leader with regards to area occupied by coral reef, estimated at 51,090 km2. The total number of coral reef fishes around 2600 species. The highest diversity of coral reef fishes with about 1766 species is Papua Barat. The dominant family is Gobiidae with 405 species, Labridae about 200 species and Pomacentridae with about 176 species. The islands in Indonesia have a unique history and inhabit by numbers of endemic fish species, i.e Muna island, Aru islands, and Enggano island. LIPI conducted an expedition in Enggano in 2015, the result indicated some undescribed species there. The new species found in Indonesia since a long time ago, started on 16 century up to now. The research on freshwater fish in Indonesia found 66 new fish species. Until now there are still a lot of unexplore waters in Indonesia, and there are still awaiting undescribe fish species to be named. Fish conservation in the small islands need to be done, as most of them migrate to the sea. Some aphidromous species growth and reproduction in freshwaters but the juvenile migrate downstream towards the sea, spreading at sea, metamorphosis phase they enter upstream migration and settlement in the freshwater.AbstrakIndonesia merupakan negara kepulauan yang terbesar di dunia, pulau-pulau ini berjajar, membentang dari Sabang sampai Merauke. Kajian jumlah pulau di Indonesia telah diteliti oleh beberapa lembaga di negeri ini. Sebagai negara kepulauan, Indonesia seolah-olah merupakan habitat laut yang tak berujung. Terumbu karang negeri ini sekitar 51.090 km2 merupakan yang terluas di dunia. Jumlah total spesies ikan di perairan terumbu karang Indonesia sekitar 2600 spesies. Wilayah tertinggi keragaman jenisnya adalah perairan Papua Barat dengan 1766 spesies. Famili yang terbesar adalah Gobiidae 405 spesies, selanjutnya Labridae 200 species dan Pomacentridae 176 spesies. Banyak pulau mempunyai riwayat unik dan dihuni oleh spesies-spesies endemik, diantaranya Pulau Muna, Pulau di Kepulauan Aru, juga Pulau Enggano. Hasil penelitian LIPI di P. Enggano mengindikasikan adanya beberapa jenis baru ikan. Penemuan spesies baru telah berlangsung lama, sejak penelitian iktiofauna di Indonesia dimulai pada abad 16 sampai saat ini. Hasil penelitian ikan air tawar di perairan Indonesia telah mendapatkan 66 spesies baru, sampai saat ini masih banyak wilayah perairan belum diteliti dan kemungkinan besar ada spesies baru yang menanti diungkap...