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Journal Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology

223 articles
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Achmad Poernomo, Farida Ariyani, Murdinah Murdinah
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 14, pp 55-64; doi:10.15578/squalen.v14i2.383

Abstract:Peptones from fish waste has been widely studied, however information about its shelf life is stilllimited. This study aims to test the storability of dried peptone from tuna and shrimp waste produced through hydrolysis using alcalase enzyme. Peptone powders were packed in HDPE plastic bottles and plastic coated aluminum foil, stored at room temperature, and periodically observed in quality (moisture content, aw, color and appearance). A test was also performed on their ability to support the growth of Staphylococcus aureusbacteria; all were compared to commercial peptone (Difco). Shrimp waste peptone had the highest moisture, ash calcium contents, while tuna peptone has the highest fat content. During five month storage at ambient temperature, all peptones experienced a slight decrease in quality. Aluminum foil performed better than HDPE bottles as a packaging material for peptones, i.e., able to maintain the moisture content, water activity, and appearance. Although the ability to support bacterial growth after five months of storage was slightly affected, the tested peptones were still able to beused as bacterial growing media. It can be concluded that fish waste peptones had comparable quality and shelf-life atambient temperature to commercial peptone.
Pujoyuwono Martosuyono, Yusro Nuri Fawzya, Gintung Patantis, Sugiyono Sugiyono
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 14, pp 85-92; doi:10.15578/squalen.v14i2.398

Abstract:Protease enzyme produced from Bacillus sp was employed to hydrolyze fish protein hydrolysates (FPH) under controlled conditions at a batch-pilot plant scale-process. Thirty kilograms of fish meat was mincedand mixed with 60 liters of water in 100 liters stainless steel vessel and 20,000 units of protease enzyme was added per kg of fish. Hydrolysis of fish was carried out at 55 oC for 6 hours. Multi stage of filtration were done to separate the FPH from unhydrolized fish residue. Mass balance were carried out to determine the rate of hydrolysis and yields. W ithout pH adjustment, 80% of substrate hydrolyzed could be achieved in 6 hour at 55 °C. Three kinds of products were recovered from the process, i.e solid residue, liquid FPH as filtration product, and spray dried FPH. Hydrolysis of 30 kg of fish meat substrate producing 1.7-2.0 kg of unhydrolyzed residue and 70 L of liquid FPH. Afterspray drying process of liquid FPH, 13 kg of FPH powder was recovered. The proximate and amino acid analysis of spray dried FPH showed that the FPH containing 20% of protein, rich in amino acids especially lysine and leucineand the residue still had 85,36% of protein (dry basis) that could be utilized for other purpose.
Mahrus Ali, Ardiansyah Kurniawan, Nuning Mahmudah Noor
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 14, pp 93-101; doi:10.15578/squalen.v14i2.386

Abstract:Kecalokis recognized as a typical shrimp sauce traditionally produced by the Malay inhabitants in Bangka Island and Palembang, Southern region of Sumatra. Comparing to terasi, the most common Indonesian fermented shrimp, the information on kecalokhas rarely been reported. This study aimed to obtain the characteristics of both the geographical origin of kecalokproduct from Bangka and Palembang. A descriptive analysis was used to observe the profiles of both kecalokproducts including sensory, microbiology, proximate, and physical analysis. Results showed that kecalokfrom both locations had similar characteristics, i.e. a distinctive taste and aroma, high nutrition value, and containing some useful bacteria (LAB) which have beneficial role in human body. Therefore, kecalokwill be a prospective functional based-indigenous food in Indonesia.
Wendy Alexander Tanod, Didit Kustantio Dewanto, Samliok Ndobe, Putut Har Riyadi, Masteria Yunovilsa Putra
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 14, pp 73-83; doi:10.15578/squalen.v14i2.394

Abstract:This study aimed to evaluate the potential antibacterial and antioxidant activities of Sinulariasp. and Sarcophyton sp. from the Palu Bay, Central Sulawesi, Indonesia. Soft corals were identified as Sinulariasp. (SC1), Sinularia sp. (SC2), andSarcophytonsp. (SC3). Antibacterial activity was examined using agar diffusion well method. Antioxidant activity was measured by the DPPH radical scavenging method. The samples were macerated in MeOH: DCM. The crude extracts were partitioned with DCM, EtOAc, and BuOH. The crude extract of Sinulariasp. (SC2) showed a very strong antibacterial activity as it was able to inhibit the growth of Staphylococcus aureusand Escherichia coliup to 10 mg/mL. Sinularia sp. (SC1) crude extract showed strong activity againstS. aureus, whereas it showed moderate against E. coli.Sarcophyton sp. (SC3) crude extract showed moderate activity against S. aureus, whereas it showed weak against E. coli. The partition fractions of the three soft coral extracts had the potential to be a potent antioxidant agent.
Fateha Fateha, Singgih Wibowo, Joko Santoso, Agusman Agusman, Uju Uju
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 14, pp 65-72; doi:10.15578/squalen.v14i2.397

Abstract:Alkali treated cottonii (ATC) is a derivative product of Eucheuma cottoniithat is treated with alkali. This study used raw material of sap-free seaweed for ATC processing. Sap-free seaweed is a seaweed which thallus has been extracted with liquid known as sap. The use of sap-free seaweed as an ATC product is constrained by its low quality, so an effort to improve its quality is needed. Therefore the obejcetive of this Research was to get the optimal conditions of ATC processing. Experimental data were designed and analyzed using Response Surface MethodologyCentral Composite Design (RSM-CCD) using Design Expert 10.0.7® program. The optimization of ATC processing involved three components that were considered influential, namely KOH concentration, temperature, and processing time. Recommendation for optimal conditions issued by RSM-CCD on ATC processing from sap-free seawee d were 6 % KOH concentration at 75 oC for 120 minutes (93.1% desirability). The results of the response analysis showed a yield of 39.47% and a gel strength of 595.32 g/cm2. As a research control, the recommendation of RSMCCD used in ATC processing from nonsap-free seaweed was obtained yield of 36.81% and gel strength of 574.44 g/cm 2. ATC from sap-free seaweed has higher yield and gel strength than that from nonsap-free seaweed. Thismight be due to the sap-free seaweed was obtained using the proper sap extraction process and ATC processingunder optimal conditions, so that the quality of seaweed was better maintained.
Squalen Bulletin
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 14; doi:10.15578/squalen.v14i1.390

Giuseppe C. Zuccarello, Nicholas A. Paul
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 14, pp 43-53; doi:10.15578/squalen.v14i1.384

Abstract:The most rapidly expanding areas for seaweed production in the world are the tropics, including Indonesia, yet these areas are also where molecular identification of local marine flora has only been sporadically employed. Furthermore, a goal for the Government of Indonesia is to diversify the types of seaweed that are being utilized, targeting valuable products and, hand in hand, to develop aquaculture techniques for these species. Morphological methods for species identification in algae are complex or unreliable, due to simple morphologies and plasticity. Therefore, it is crucial that the correct identification is made for species and varieties of commercial interest so that growth and biochemical results can be compared and contrasted between locations, across environments and over time without taxonomic ambiguity. This guide presents entry level methodologies for sample collection, DNA preservation, DNA extraction, PCR, and analyses of DNA sequence data, as a first step in the genetic characterization of both well-known cultivated species and identification of different species with potential economic properties.
Squalen Bulletin
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 14; doi:10.15578/squalen.v14i1.389

Umi Anissah, Ajeng Kurniasari Putri, Giri Rohmad Barokah
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 14, pp 1-8; doi:10.15578/squalen.v14i1.369

Abstract:The demand for Indonesian opah fish as an export product is increasing in the international market. Three countries (Malaysia, Mauritius, and Taiwan) recorded as the leading export destination of Indonesian opah fish. However, as the fish kept in a frozen state during export transportation, the endogenous formaldehyde may increase over time. This research presented the health risk assessment of population in the leading export destination countries that consumed opah fish from Indonesia. The study aimed to reveal the most potential export destination country that may accept an increasing volume of opah fish supply from Indonesia. The potency was determined from current export volume, the amount of endogenous formaldehyde content, and fish consumption at each country. The data were calculated with @Risk®7.0 software. The results showed opah fish consumed by Malaysian can be categorized as safe. Increasing the number of opah fish imported by Malaysian as much as six times, 12 times, 18 times, 27 and 36 times relatively does not cause health risks related to the presence of its endogenous formaldehyde. Moreover, opah fish consumed by Taiwanese is also safe, but with increasing the number of consumptions by more than 26 times is suspected to be potentially causing a health problem. However, opah fish consumed in Mauritius was categorized as unsafe and potentially caused health risks. Based on these results, Indonesia may consider to increase the opah fish export to Malaysia and Taiwan in the future.
Endar Marraskuranto, Tri Joko Raharjo, Rina Sri Kasiamdari, Tri Rini Nuringtyas
Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology, Volume 14, pp 21-31; doi:10.15578/squalen.v14i1.379

Abstract:Rhodomonas salina produces Cr-phycoerythrin545 as its designated phycoerythrin (PE) with an absorption maximum at 545 nm and a shoulder 564 nm. PE has potential to be applied as colorants, pharmaceutical agents, and fluorescent dye tags. The stability of the PE color is influenced by the physicochemical factors of the solution. This study aimed to analyze the color stability of PECE against chemical (ethanol and pH) and physical (light and temperature) factors. PECE was prepared from freeze-dried biomass of R. salina and was extracted in phosphate buffer solution (pH = 6.0) using a freeze-thaw method in -25 oC (2 hours) and 4 oC (24 hours). The resulting extract was concentrated and dried in a freeze-dryer. Analyses were conducted using UV-visible and fluorescence spectrophotometer. PECE showed color stability against light of white fluorescent lamp exposure up to 8 hours, temperature exposure up to 40 oC, ethanol solution up to concentration of 20 % (v/v), and pH range 3.9-8.42. Results from this study can be useful for extraction, purification, and future application of Cr-PE545.
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