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(searched for: doi:10.18488/journal.ar/2016.3.1/101.1.10.21)
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, , Arum Setiawan, Veronika Larasati
Published: 27 November 2018
E3S Web of Conferences, Volume 68; https://doi.org/10.1051/e3sconf/20186801002

Abstract:
Swamp buffalo (Bubalusbubalis) is one of the endemic species that become a wealth of genetic resources of South Sumatra. This study aims to the genetic variation and relationships of kinship 6 variants of swamp buffalo South Sumatera. The methods used by the molecular approach using RAPD-PCR primer 5 i.e. ILO 1204, ILO 1212, ILO 525, OPW 03 and OPY 13. Data was analyzed using SPSS ver 16.0 and presented in dendrogram. The results of the amplification, all primary produce band with a total of 63 band of DNA (14.92%) with an average of every primary produce 12.6 band of DNA. The most primary produce DNA polymorphic bands namely OPW 03 (23.81%) and ILO 1204 (20.63%), while the primary ILO 525 (0.00%) do not generate polymorphic bands. Genetic variation of swamp buffalo has a low genetic variation with 14.92% percentage it generated polymorphic bands. The results of the dendogram obtained two clusters namely cluster 1 included Kerbau Tanduk Bulat, Kerbau Tanduk Langit, Kerbau Tanduk Melintang and Kerbau Tanduk Dungkul, while the cluster 2 of them Kerbau Bule and Kerbau Rebah Belakang. Swamp buffalo variants that have the closest genetic distance. Kerbau Tanduk Langit and Kerbau Tanduk Bulat with 856 coefficient similarity, while the farthest Kerbau Tanduk Langit and Kerbau Bule with the coefficient similarity -972. Swamp buffalo (Bubalusbubalis) of South Sumatera, which consists of 6 variants of buffalo have low genetic variation and inbreeding of closekinship.
, , Rizki Wahyudi
Published: 1 January 2017
AIP Conference Proceedings; https://doi.org/10.1063/1.5011530

Abstract:
Swamp buffalo (Bubalus bubalis) is an endemic species and one of the genetic wealth of South Sumatra with a distribution area in the district of Pampangan (OganIlir and OganOganIlir). Suspected inbreeding causes decreased phenotypic properties. Inbreeding among various swamp buffalo is certainly not only lower the qualities but also genotypes and phenotypes. It is of interest to determine kinship variants swamp buffaloes from Pampangan through the analysis of a blood protein profile. Blood protein profile of four variants swamps buffalo was studied by using five electrophoresis system i.e. pre-albumin (Palb), albumin (Alb), ceruloplasmin (Cp), transferrin (Tf) and transferrin post (Ptf). In this paper, it is obtained that there was no significant differences among the four variants of the buffaloes were used as a sample. Prealbumin has two alleles (Palb1 and Palb2), albumin has three alleles (Alba, AlbB, AlbC), ceruloplasmin has one allele (BPA), post-transferrin has one allele (PTFA) with an allele frequency 1.0000 at any time transferrin has two alleles (TFA and TFB) with the allele frequency of 0.7500 and 1.0000. Characteristics prealbumin (Palb), albumin (Alb), ceruloplasmin (Cp), and post-transferrin (P-tf) is monomorphic, while transferrin is polymorphic average heterozygosity values all loci (H) 0.1286. Based on average heterozygosity, the swamp buffalo (Bubalusbubalis) from Pampangan has low genetic variation and closest genetic relationship.
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