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(searched for: doi:10.1016/j.bej.2015.07.002)
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E. V. Bizina, , N. I. Zolotareva, S. S. Grazhulene, T. N. Ermolaeva
Published: 12 April 2022
Journal of Analytical Chemistry, Volume 77, pp 458-465;

A piezoelectric immunosensor with a recognition layer based on magnetic carbon nanocomposites is developed for the determination of ciprofloxacin. The receptor coating of the sensor is formed by the action of a magnetic field on magnetic particles located on the surface of carbon nanotubes modified with a ciprofloxacin conjugate. The sizes of magnetic particles in the composition of the nanocomposite are determined by scanning electron microscopy. A dependence of the mass of the recognition coating on the size of magnetic particles on the surface of carbon nanotubes is shown. A detection cell with a sensor located above a neodymium magnet is proposed. The analytical characteristics of the immunosensor are determined, the limit of detection for ciprofloxacin is 2 ng/mL, and the linear range of determined concentrations is 5–400 ng/mL. The use of magnetic carbon nanocomposites in the creation of a recognition layer ensures the reduction of the time of sensor preparation to analysis from 24 to 1.5 h and extends its service life. The sensor is tested in the detection of antibiotics in milk and meat.
Published: 17 July 2021
Journal: Toxins
Mycotoxin contamination of cereals used for feed can cause intoxication, especially in farm animals; therefore, efficient analytical tools for the qualitative and quantitative analysis of toxic fungal metabolites in feed are required. Current trends in food/feed analysis are focusing on the application of biosensor technologies that offer fast and highly selective and sensitive detection with minimal sample treatment and reagents required. The article presents an overview of the recent progress of the development of biosensors for deoxynivalenol and zearalenone determination in cereals and feed. Novel biosensitive materials and highly sensitive detection methods applied for the sensors and the application of these sensors to food/feed products, the limit, and the time of detection are discussed.
Ruchika Chauhan, Rashi Bhardwaj, Sheetal K. Bharadwaj, Ajit Kaushik, Rajshekhar Karpoormath,
Published: 13 March 2021
The publisher has not yet granted permission to display this abstract.
, Julker Nine, Filipe Samuel Silva
Published: 3 February 2021
Advances in colloid and interface science, Volume 290;

The publisher has not yet granted permission to display this abstract.
Published: 16 December 2020
Food and Chemical Toxicology, Volume 148;

This work focuses on the study of nanomaterial-based sensors for mycotoxins detection. Due to their adverse effects on humans and animals, mycotoxins are heavily regulated, and the foodstuff and feed stocks with a high probability of being contaminated are often analyzed. In this context, the recent developments in graphene-based electrochemical sensors for mycotoxins detection were examined. The mycotoxins' toxicity implications on their detection and the development of diverse recognition elements are described considering the current challenges and limitations.
Avinash Kumar Singh, Tarun Kumar Dhiman, Lakshmi G.B. V.S.,
Published: 13 October 2020
Bioelectrochemistry, Volume 137;

The publisher has not yet granted permission to display this abstract.
Published: 19 August 2020
Journal: Nanomaterials
Nanomaterials, Volume 10;

Aflatoxins are the secondary metabolites of Aspergillus flavus and Aspergillus parasiticus and are highly toxic and carcinogenic, teratogenic and mutagenic. Ingestion of crops and food contaminated by aflatoxins causes extremely serious harm to human and animal health. Therefore, there is an urgent need for a selective, sensitive and simple method for the determination of aflatoxins. Due to their high performance and multipurpose characteristics, nanomaterials have been developed and applied to the monitoring of various targets, overcoming the limitations of traditional methods, which include process complexity, time-consuming and laborious methodologies and the need for expensive instruments. At the same time, nanomaterials provide general promise for the detection of aflatoxins with high sensitivity, selectivity and simplicity. This review provides an overview of recent developments in nanomaterials employed for the detection of aflatoxins. The basic aspects of aflatoxin toxicity and the significance of aflatoxin detection are also reviewed. In addition, the development of different biosensors and nanomaterials for aflatoxin detection is introduced. The current capabilities and limitations and future challenges in aflatoxin detection and analysis are also addressed.
, Chanchan Ren, Chengquan Wang, Keqi An, Haining Cui, Nan Hao,
Published: 24 July 2020
Biosensors and Bioelectronics, Volume 166;

The publisher has not yet granted permission to display this abstract.
Y.-H. Zha, Y. Zhou
Published: 6 March 2020
World Mycotoxin Journal, Volume 13, pp 151-162;

Aflatoxin B1 (AFB1) is highly carcinogenic, mutagenic and teratogenic. Accordingly, sensitive, rapid and cost-effective techniques for detection of AFB1 is in urgent demand for food safety and the health of consumers. In this review, we report the current state of immunoassay formats and development, mainly based on nanomaterials for determination of AFB1. Following an introduction of the field, the microplate-, membrane- and microelectrode-based immunoassays are described. The relevant mechanisms, sensitivities, superiorities and deficiencies of each format are discussed. Finally, perspectives on the future development of nanomaterials-based immunoassays for AFB1 are provided.
Muhammad Rizwan Younis, , Xing‐Hua Xia
Published: 14 February 2020
The publisher has not yet granted permission to display this abstract.
Somayeh Mousavi Nodoushan, , Reza Kachuei, Abbas Ali Imani Fooladi
Published: 30 October 2019
Analytical Methods, Volume 11, pp 6033-6042;

An electrochemical aptasensor was developed for the detection of aflatoxin B1 using a nanocomposite of graphene oxide and gold nanowires.
Z. Bujňáková, O. Shpotyuk, E. Dutková, E. Tóthová, J. Kováč, , M. Baláž, P. Baláž
Published: 5 July 2019
The publisher has not yet granted permission to display this abstract.
Hongsu Wang, Yi Xiu, Yan Chen, Liping Sun, Libin Yang, Honghao Chen, Xiaodi Niu
Published: 24 May 2019
Journal: RSC Advances
RSC Advances, Volume 9, pp 16278-16287;

The outbreak of food-borne pathogens has become a serious concern; therefore, the detection of pathogenic bacteria in food is required. Untreated, sensitive, and reliable sensors should be developed for the detection of Staphylococcus aureus (S. aureus). In this study, a sensitive antibody-based electrochemical immunosensor was developed using antibody (Ab)-hierarchical mesoporous silica (HMS) bio-conjugates for label-free detection of low concentrations of S. aureus. First, a bio-template method based on butterfly wings was used to prepare the HMS. Then, the carrier material was amino-functionalized to cross-link the antibody with glutaraldehyde. The Ab-HMS bio-conjugates were then immobilized on a glassy carbon electrode (GCE), and the presence of S. aureus was detected by analyzing the changes in the peak currents after the antigen–antibody complex formation. Differential pulse voltammetry (DPV) was performed with bacterial concentrations ranging from 10 to 2 × 103 colony forming units (CFU) mL−1. Selective tests were performed using Escherichia coli (E. coli), Listeria monocytogenes (L. monocytohenes), and Salmonella, and the selective assays showed specific detection of S. aureus using the sensor. In addition, the immunosensor showed a good linear relationship between the peak current increase and logarithmic S. aureus concentration (R2 = 0.9759) with a fast detection time (20 min) and detection limit of 11 CFU mL−1. When the electrochemical impedance spectroscopy (EIS) was performed under the same conditions, the results showed a good linear relationship between the impedance change value and the bacterial concentration (R2 = 0.9720), the limit of detection (LOD) was 12 CFU mL−1. The performance of the sensor was compared with that of the colony counting method in the spiked milk sample test. The results showed no significant difference in the test results. Hence, this electrochemical immunosensor can be used to quickly detect S. aureus in actual food samples with a high sensitivity, specificity and stability.
, Daria Yarynka, Elena Piletska, Rostyslav Linnik, Olga Zaporozhets, Oleksandr Brovko, Sergey Piletsky, Anna El'Skaya
Published: 9 April 2019
Journal: Talanta
Talanta, Volume 201, pp 204-210;

A novel smartphone-based optical biomimetic sensor based on free-standing molecularly imprinted polymer (MIP) membranes was developed for rapid and sensitive point-of-care detection of aflatoxin B1. The developed MIP membranes were capable of selective recognition of the target analyte and, at the same time, of generation of a fluorimetric sensor response, which could be registered using the camera of a smartphone and analysed using image analysis. The developed system provides a possibility of synchronous detection of aflatoxin B1 in 96 channels. UV irradiation of aflatoxin B1, selectively bound by the MIP membranes from the analysed samples, initiated fluorescence of aflatoxin B1 with intensity directly proportional to its concentration. The composition of the MIP membranes used as a recognition element was optimised taking into account data of computational modelling. Two functional monomers (2-acrylamido-2-methyl-1-propansulfonic acid and acrylamide) were identified as optimal for the formation of aflatoxin B1-selective binding sites in the structure of the MIP membranes. Working characteristics of the smartphone-based sensor system were also estimated. The influence of pH and of buffer and NaCl concentrations on the smartphone-based sensor responses were studied. High selectivity of the developed sensor system towards aflatoxin B1 was confirmed in experiments with close structural analogues of the target analyte - aflatoxin G2, and ochratoxin A. The detection limit for aflatoxin B1 using the smartphone-based sensor systems was found to be 20 ng mL−1 for the sensor based on MIP membranes synthesised with acrylamide as a functional monomer. The storage stability of the recognition elements of the developed sensors was estimated as one year when stored at 22 °C. The possibility to detect the aflatoxin B1 in contaminated food samples was shown. The MIP-membrane-based sensor system provided a convenient point-of-care approach in food safety testing.
Baoling Liu, Hongchen Zhang, Ya Ding
Published: 5 December 2018
Chinese Chemical Letters, Volume 29, pp 1725-1730;

The publisher has not yet granted permission to display this abstract.
Ying Tang, , Jin Zhang,
Published: 1 November 2018
Analytica chimica acta, Volume 1031, pp 161-168;

A simple and sensitive quartz crystal microbalance (QCM) immunosensing platform was designed for the high-efficient detection of aflatoxin B1 (AFB1) in foodstuff. Initially, phenoxy-derived dextran molecule was immobilized on the surface of QCM gold substrate by using thiolated β-cyclodextrin based on the supramolecular host-guest chemistry between phenoxy group and cyclodextrin. Then, AFB1-bovine serum albumin (AFB1-BSA)-conjugated concanavalin A (Con A) was assembled onto the QCM probe through the dextran-Con A interaction. Glucose-loaded nanoliposome, labeled with monocolonal anti-AFB1 antibody, was used for the amplification of QCM signal. Upon target AFB1 introduction, the analyte competed with the immobilized AFB1-BSA on the probe for the labeled anti-AFB1 antibody on the nanoliposome. Based on specific antigen-antibody reaction, the amount of the conjugated nanoliposomes on the QCM probe gradually decreased with the increment of target AFB1 in the sample. Upon injection of Triton X-100 in the detection cell, the carried nanoliposome was lysed to release the encapsulated glucose molecules. Thanks to the stronger affinity of Con A toward glucose than that of dextran, AFB1-BSA-labeled Con A was displaced from the QCM probe, resulting in the change of the local frequency. Under the optimum conditions, the shift of the functionalized QCM immunosensing interface in the frequency shift was proportional to the concentration of target AFB1 within a dynamic range from 1.0 ng kg−1 to 10 μg kg-1 at a low detection limit of 0.83 ng kg−1. In addition, the acceptable assayed results on precision, reproducibility, specificity and method accuracy for the analysis of real samples were also acquired. Importantly, our strategy can provide a signal-on competitive immunoassay for the detection of small molecules, e.g., mycotoxins and biotoxins, thereby representing a versatile sensing schemes by controlling the corresponding antibody or hapten in the analysis of food safety.
Nina Naquiah Ahmad Nizar, Ismat Hazim Zainal, Sharmin Quazi Bonny, Thiruchelvi Pulingam, Lavanya Malini Vythalingam, Eaqub Ali
Published: 21 September 2018
The publisher has not yet granted permission to display this abstract.
K. Yugender Goud, , , , S.E. Lee, K. Vengatajalabathy Gobi,
Published: 16 August 2018
Biosensors and Bioelectronics, Volume 121, pp 205-222;

Nanomaterial-embedded sensors have been developed and applied to monitor various targets. Mycotoxins are fungal secondary metabolites that can exert carcinogenic, mutagenic, teratogenic, immunotoxic, and estrogenic effects on humans and animals. Consequently, the need for the proper regulation on foodstuff and feed materials has been recognized from times long past. This review provides an overview of recent developments in electrochemical sensors and biosensors employed for the detection of mycotoxins. Basic aspects of the toxicity of mycotoxins and the implications of their detection are comprehensively discussed. Furthermore, the development of different molecular recognition elements and nanomaterials required for the detection of mycotoxins (such as portable biosensing systems for point-of-care analysis) is described. The current capabilities, limitations, and future challenges in mycotoxin detection and analysis are also addressed.
, Hongbo Wang, , Zhenguo Ji
Published: 1 June 2018
Materials Research Express, Volume 5;

The detection of aflatoxin B1 (AFB1) using immunoassays, especially electrochemical immunoassays, is fast, sensitive and efficient. In this study, 2-aminoethanethiol was used to enhance the speed and sensitivity of conventional electrochemical immunoassays for AFB1 detection by assembly on the surface of a Au electrode, forming self-assembled monolayers (SAMs). Then, non-competitive immunoassays occurred on the modified electrode surface forming an electrochemical immunoassay sensor. X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared (FTIR) spectroscopy were used to examine the state of the SAMs, and an electrochemical workstation was used to monitor the current change of the electrochemical reaction, so as to characterize the designed immunosensor. Our experimental results shows that, the 2-aminoethanethiol reagent was successfully assembled on the Au surface through Au-S bonding and the –NH2 terminal group faced outward. Herein, the minimum concentration of AFB1 which caused a significant current change was 0.01 ng/mL. The prepared immunosensor also exhibited excellent stability and sensitivity after storage for 7 days or upon regeneration.
Yun Wang,
Published: 1 April 2017
Current opinion in biotechnology, Volume 44, pp 74-86;

As far as chemical analysis is concerned, foods are among the most difficult matrices to work with because they are complex, heterogeneous substances with a high degree of variety. Assaying foods for trace levels of chemical and microbiological substances is a challenge that often requires the application of time-consuming, expensive analytical instrumentation in dedicated facilities populated by highly trained personnel. Therefore there is a continued demand for new analytical technologies that can detect small concentrations of chemicals or microbes in a more cost- and time-effective manner, preferably in the field, on the production line, and/or non-destructively, with little to no sample pre-treatment, and possibly by individuals with scant scientific training. In the last decade, nanotechnology - a branch of science that takes advantage of the unique chemical and physical properties of matter on the nanoscale - has created new opportunities for both qualitative and quantitative detection of vapors/gasses, small molecules, biopolymers, and even living microbes in a fraction of the time and expense of traditional analytical techniques. This article offers a focused review of recent progress in nanotechnology-enabled biosensing as applied to foods and related matrices, paying particular attention to trends in the field, recent breakthroughs, and current areas of need. Special focus is paid to two primary categories of nanobiosensors - optical and electrochemical - and the discussion includes a comparison of their various strengths and weaknesses as they pertain ensuring the safety of the food supply.
K. Yugender Goud, , , , K. Vengatajalabathy Gobi, ,
Published: 1 September 2016
Analytical biochemistry, Volume 508, pp 19-24;

In this study, a simple TAMRA (tetramethyl-6-carboxyrhodamine) quenching based aptasenisng platform was designed for the detection of aflatoxin B1 (AFB1). Herein, we compared the analytical performance of two aptamer sequences (seqA & seqB). The AFB1 detection was based on the interactions of FAM (carboxyfluorescein) labelled aptamer with TAMRA labelled DNA complementary strand in the presence and absence of target analyte. Under optimized experimental condition, TAMRA labelled strand quenched the fluorescence response of FAM labelled aptamer due to the non-covalent interaction between the two DNA strands. The binding of AFB1 induced the complex formation, weakened the interaction between FAM-labelled aptamer and TAMRA labelled complementary strand, resulting in the fluorescence recovery. By using this principle concept, an assay was constructed for the detection of AFB1. The method exhibited good sensitivity, selectivity with a limit of detection 0.2 ng mL-1, and a wide linear range from 0.25 ng mL-1 to 32 ng mL-1. For real sample application, the aptasensors were tested in beer and wine samples with a good recovery rates obtained for AFB1 detection.
Published: 4 March 2016
Biosensors and Bioelectronics, Volume 81, pp 532-545;

The publisher has not yet granted permission to display this abstract.
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