A biosensor platform based on Au/MXene nanocomposite for sensitive enzymatic glucose detection is reported. The biosensor leverages the unique electrocatalytic properties and synergistic effects between Au nanoparticles and MXene sheets. An amperometric glucose biosensor is fabricated by the immobilization of glucose oxidase (GOx) enzyme on Nafion solubilized Au/ MXene nanocomposite over glassy carbon electrode (GCE). The biomediated Au nanoparticles play a significant role in facilitating the electron exchange between the electroactive center of GOx and the electrode. The GOx/Au/MXene/Nafion/GCE biosensor electrode displayed a linear amperometric response in the glucose concentration range from 0.1 to 18 mM with a relatively high sensitivity of 4.2 μAmM⁻¹ cm⁻² and a detection limit of 5.9 μM (S/N = 3). Furthermore, the biosensor exhibited excellent stability, reproducibility and repeatability. Therefore, the Au/MXene nanocomposite reported in this work is a potential candidate as an electrochemical transducer in electrochemical biosensors.

A novel platinum–palladium nanoparticle-decorated carbon nanofiber nanocomposite was greenly prepared by attaching the nanoparticles to the nanofibers for the preparation of glucose biosensors. The platinum–palladium bimetallic nanoparticle-modified carbon nanofiber nanocomposite was characterized by transmission electron microscopy, X-ray diffraction, cyclic voltammetry, and electrochemical impedance spectroscopy. The glucose biosensor was constructed by immobilizing glucose oxidase on the nanocomposite-modified glassy carbon electrode by cross-linking with glutaraldehyde. The resulting biosensor exhibited a good response to glucose with a wide linear range from 2.5 × 10⁻⁶ to 1.3 × 10⁻² M with a high sensitivity of 154.6 µA mM⁻¹ cm⁻² and a detection limit of 0.7 µM. This biosensor was shown to offer good accuracy, precision, and reproducibility. The determination of glucose in human serum by the modified electrode was in good agreement with standard values. The nanocomposite electrode offers a suitable platform for the determination of glucose.