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(searched for: doi:10.4236/ojab.2013.21001)
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Published: 30 March 2022
Abstract:
The current chapter is focused on biopolymers and Bionanocomposite as environmentally friendly materials, modeling of the production processes, and coating of bio-textiles. Different industries use biopolymers and Bionanocomposite in for the current environmental applications. Furthermore, composition and classification of biopolymers, the theoretical methods, and factorial experimental designs (FED) for optimization and modeling processes of the environmentally friendly textiles used as an alternative to traditional chemical textile products with zero to low environmental footprint are studied at acceptable cost. This chapter will also describe the novel optimization, experimental factorial design, and how the novel modeling methods will help less experienced polymer designers in taking the best experimental decision controlled by the design factors. It also discusses how the fully biodegradable polymers support the industry by decreasing the processing energy, material and manufacturing costs. Finally there are an overview of the current and future developments of biodegradable polymers applications in modern bio-textiles industries.
Swati Chaudhary, Niloy Sarkar, Mahima Kaushik
Published: 26 February 2021
The publisher has not yet granted permission to display this abstract.
Iris Cagnasso, , , , , , Francesca Durbiano
Published: 27 June 2019
RSC Advances, Volume 9, pp 19844-19854; https://doi.org/10.1039/c9ra02086a

Abstract:
The OTA degradation process becomes irreversible only if, following hydrolyzation of the lactone ring, the molecule fragments.
Published: 23 May 2019
by MDPI
Abstract:
The detection of mycotoxins is crucial because of their toxicity in plants, animals, and humans. It is very important to determine whether food products are contaminated with mycotoxins such as ochratoxin A (OTA), as mycotoxins can survive heat treatments and hydrolysis. In this study, we designed a fluorescence resonance energy transfer (FRET)-based system that exploits antibody-antigen binding to detect mycotoxins more rapidly and easily than other currently available methods. In addition, we were able to effectively counteract the matrix effect in the sample by using a nitrocellulose membrane that enabled fluorescence measurement in coffee samples. The developed FRET on lateral flow immunoassay (FRET-LFI) system was used to detect OTA at a limit of detection (LOD) of 0.64 ng∙mL−1, and the test can be completed in only 30 min. Moreover, OTA in coffee samples was successfully detected at a LOD of 0.88 ng∙mL−1, overcoming the matrix effect, owing to the chromatographic properties of the capillary force of the membrane. We believe that the developed system can be used as a powerful tool for the sensitive diagnosis of harmful substances such as mycotoxins and pesticides for environmental and food quality control monitoring.
Tarun K. Dhiman, Gbvs Lakshmi, Appan Roychoudhury, Sandeep K. Jha,
Published: 1 May 2019
ChemistrySelect, Volume 4, pp 4867-4873; https://doi.org/10.1002/slct.201803752

The publisher has not yet granted permission to display this abstract.
Published: 11 April 2018
by MDPI
Sensors, Volume 18; https://doi.org/10.3390/s18041161

Abstract:
Ochratoxin A (OTA) is a potent mycotoxin that poses a risk in food and feed moieties and subject to worldwide regulation. Laboratory-based analytical methods are traditionally employed for reliable OTA quantification, but these methods cannot provide rapid and on-site analysis, where biosensors fill this gap. In this study a label-free quartz crystal microbalance (QCM)-based immunosensor for the detection of OTA, which is one of the most important small molecule contaminants, was developed by direct immobilization of OTA to amine-bearing sensor surfaces using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC)/N-Hydroxysuccinimide (NHS) chemistry. The protein-free sensor surface enabled regeneration of sensor surface with 50 mM NaOH and 1% SDS up to 13 times without loss of performance, which would disrupt a protein-containing sensor surface. We developed a QCM immunosensor using the developed sensor surface with a 17.2–200 ng/mL detection range which can be used for on-site detection of feedstuffs.
, Alexandr G. Burenin, Natalia G. Massarskaya, Alexey V. Betin, ,
Sensors and Actuators B: Chemical, Volume 246, pp 1080-1084; https://doi.org/10.1016/j.snb.2016.12.071

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Published: 13 May 2017
by MDPI
Abstract:
Biosensing platforms based on peptide recognition provide a cost-effective and stable alternative to antibody-based capture and discrimination of ochratoxin-A (OTA) vs. ochratoxin-B (OTB) in monitoring bioassays. Attempts to engineer peptides with improved recognition efficacy require thorough structural and thermodynamic characterization of the binding-competent conformations. Classical molecular dynamics (MD) approaches alone do not provide a thorough assessment of a peptide’s recognition efficacy. In this study, in-solution binding properties of four different peptides, a hexamer (SNLHPK), an octamer (CSIVEDGK), NFO4 (VYMNRKYYKCCK), and a 13-mer (GPAGIDGPAGIRC), which were previously generated for OTA-specific recognition, were evaluated using an advanced MD simulation approach involving accelerated configurational search and predictive modeling. Peptide configurations relevant to ochratoxin binding were initially generated using biased exchange metadynamics and the dynamic properties associated with the in-solution peptide–ochratoxin binding were derived from Markov State Models. Among the various peptides, NFO4 shows superior in-solution OTA sensing and also shows superior selectivity for OTA vs. OTB due to the lower penalty associated with solvating its bound complex. Advanced MD approaches provide structural and energetic insights critical to the hapten-specific recognition to aid the engineering of peptides with better sensing efficacies.
, Sudheer Kumar, Alok K. Srivastava
Published: 31 August 2016
Environmental Chemistry Letters, Volume 15, pp 7-13; https://doi.org/10.1007/s10311-016-0580-4

The publisher has not yet granted permission to display this abstract.
, Pallavi Rai, Shikha Sharma, Hillol Chakdar, Sudheer Kumar, K. Pandiyan, Alok K. Srivastava
Published: 19 August 2016
The publisher has not yet granted permission to display this abstract.
The Journal of The Textile Institute, Volume 108, pp 674-682; https://doi.org/10.1080/00405000.2016.1180731

Abstract:
This article is focused on biopolymers as intelligent environmentally friendly polymers, the production processes, and coatings of biotextiles used in different industries for next generation environmental applications. Furthermore, classification and composition of biodegradable polymers, the theoretical techniques, and factorial experimental designs for the optimization of processes with intelligent biotextiles used as an alternative to commercial chemical-based textiles at reasonable cost with a zero to low environmental footprint are discussed. This article will also provide how these novel modeling methodologies will assist polymer designers in making the best decision. The present work also discusses how the fully biodegradable polymers support the textiles industry by decreasing the processing energy, material, and manufacturing costs. Finally, current development as well as potential future applications and trends of biodegradable polymers in modern biodegradable textiles industry will be presented.
Nawel Mejri-Omrani, Anna Miodek, Becem Zribi, Mouna Marrakchi, Moktar Hamdi, Jean-Louis Marty,
Published: 1 May 2016
Analytica Chimica Acta, Volume 920, pp 37-46; https://doi.org/10.1016/j.aca.2016.03.038

Abstract:
Ochratoxin A (OTA) is a carcinogenic mycotoxin that contaminates food such as cereals, wine and beer; therefore it represents a risk for human health. Consequently, the allowed concentration of OTA in food is regulated by governmental organizations and its detection is of major agronomical interest. In the current study we report the development of an electrochemical aptasensor able to directly detect trace OTA without any amplification procedure. This aptasensor was constructed by coating the surface of a gold electrode with a film layer of modified polypyrrole (PPy), which was thereafter covalently bound to polyamidoamine dendrimers of the fourth generation (PAMAM G4). Finally, DNA aptamers that specifically binds OTA were covalently bound to the PAMAM G4 providing the aptasensor, which was characterized by using both Atomic Force Microscopy (AFM) and Surface Plasmon Resonance (SPR) techniques. The study of OTA detection by the constructed electrochemical aptasensor was performed using Electrochemical Impedance Spectroscopy (EIS) and revealed that the presence of OTA led to the modification of the electrical properties of the PPy layer. These modifications could be assigned to conformational changes in the folding of the aptamers upon specific binding of OTA. The aptasensor had a dynamic range of up to 5 μg.L-1 of OTA and a detection limit of 2 ng.L-1 of OTA, which is below the OTA concentration allowed in food by the European regulations. The efficient detection of OTA by this electrochemical aptasensor provides an unforeseen platform that could be used for the detection of various small molecules through specific aptamer association.
Juan Li, Hong Duan, Peng Xu, ,
Published: 1 March 2016
RSC Advances, Volume 6, pp 26178-26185; https://doi.org/10.1039/C6RA03695C

Abstract:
Effect of different-sized spherical gold nanoparticles grown layer by layer on the sensitivity of immunochromatographic assay.
Lihua Lu, Modi Wang, Li-Juan Liu, ,
ACS Applied Materials & Interfaces, Volume 7, pp 8313-8318; https://doi.org/10.1021/acsami.5b01702

Abstract:
A library of six luminescent Ir(III) complexes were synthesized and studied for their capacity to function as probes for G-quadruplex DNA. The novel Ir(III) complex 1 was discovered to be selective for G-quadruplex structures and was subsequently used for the construction of a label-free G-quadruplex-based ochratoxin A (OTA) sensing platform in aqueous solution. The assay exhibited linearity for OTA in the range of 0 to 60 nM (R2 = 0.9933), and the limit of detection for OTA was 5 nM. Furthermore, this assay was highly selective for OTA over its structurally related analogues.
Mohammad A. Khiyami, Hassan Almoammar, , Mousa A. Alghuthaymi,
Biotechnology & Biotechnological Equipment, Volume 28, pp 775-785; https://doi.org/10.1080/13102818.2014.960739

Abstract:
Plant diseases are among the major factors limiting crop productivity. A first step towards managing a plant disease under greenhouse and field conditions is to correctly identify the pathogen. Current technologies, such as quantitative polymerase chain reaction (Q-PCR), require a relatively large amount of target tissue and rely on multiple assays to accurately identify distinct plant pathogens. The common disadvantage of the traditional diagnostic methods is that they are time consuming and lack high sensitivity. Consequently, developing low-cost methods to improve the accuracy and rapidity of plant pathogens diagnosis is needed. Nanotechnology, nano particles and quantum dots (QDs) have emerged as essential tools for fast detection of a particular biological marker with extreme accuracy. Biosensor, QDs, nanostructured platforms, nanoimaging and nanopore DNA sequencing tools have the potential to raise sensitivity, specificity and speed of the pathogen detection, facilitate high-throughput analysis, and to be used for high-quality monitoring and crop protection. Furthermore, nanodiagnostic kit equipment can easily and quickly detect potential serious plant pathogens, allowing experts to help farmers in the prevention of epidemic diseases. The current review deals with the application of nanotechnology for quicker, more cost-effective and precise diagnostic procedures of plant diseases. Such an accurate technology may help to design a proper integrated disease management system which may modify crop environments to adversely affect crop pathogens.
, Saurabh Srivastava, , Chandan Singh
Applied Biochemistry and Biotechnology, Volume 174, pp 880-896; https://doi.org/10.1007/s12010-014-0993-0

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