Drought is one of the major constraints to global crop production. A number of sustainable systems have focused on the development of environmentally friendly innovative biotechnological interventions to prevent yield losses. The use of essential oils as a seed priming agent can make an important contribution as a natural stimulant in increasing drought stress tolerance. This study focuses on the effects of seeds coated with different doses (D₀ (0%), D₁ (0.01%), D₂ (0.05%), D₃ (0.10%) and D₄ (0.25%)) of sage, rosemary and lavender essential oils on wheat germination, seedling establishment and yield parameters. Turkey’s local wheat genotype Köse was used as plant material. The impact of the seed priming on germination rate, coleoptile length, shoot length, root length, shoot fresh and dry weight, root fresh and dry weight, relative water content (RWC), proline, and chlorophyll contents was assessed in laboratory experiments. In addition, the effect of essential oil types on yield parameters and agronomic components (plant height, spike height, number of grains per spike, grain yield per spike, grain yield per unit area, thousand-grain weight) was evaluated in a field experiment during the 2019–2020 crop seasons in a semi-arid climate. According to laboratory results, the highest germination rate among all treatment doses was determined in the D₂ treatment (rosemary 93.30%, sage 94.00% and lavender 92.50%), while the lowest germination rates for all essential oil types were determined in the D₄ treatment (rosemary 41.70%, sage 40.90% and lavender 40.90%). Increasing treatment doses showed a similar suppressive effect on the other parameters. In the field experiment, the highest grain yield (256.52 kg/da) and thousand-grain weight (43.30 g) were determined in the rosemary treatment. However, the priming treatment has an insignificant on the number of grains per spike and the spike length. The light of these results, the effects of essential oil types and doses on yield parameters were discussed. The findings highlight the importance of using essential oils in seed priming methods for sustainable agricultural practices.

Background: Soil salinization and alkalization are widespread environmental problems that limit grapevine (Vitis vinifera L.) growth and yield. However, little is known about the response of grapevine to alkali stress. This study investigated the differences in physiological characteristics, chloroplast structure, transcriptome, and metabolome in grapevine plants under salt stress and alkali stress. Results: We found that grapevine plants under salt stress and alkali stress showed leaf chlorosis, a decline in photosynthetic capacity, a decrease in chlorophyll content and Rubisco activity, an imbalance of Na⁺ and K⁺, and damaged chloroplast ultrastructure. Fv/Fm decreased under salt stress and alkali stress. NPQ increased under salt stress whereas decreased under alkali stress. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment showed the differentially expressed genes (DEGs) induced by salt stress and alkali stress were involved in different biological processes and have varied molecular functions. The expression of stress genes involved in the ABA and MAPK signaling pathways was markedly altered by salt stress and alkali stress. The genes encoding ion transporter (AKT1, HKT1, NHX1, NHX2, TPC1A, TPC1B) were up-regulated under salt stress and alkali stress. Down-regulation in the expression of numerous genes in the ‘Porphyrin and chlorophyll metabolism’, ‘Photosynthesis-antenna proteins’, and ‘Photosynthesis’ pathways were observed under alkali stress. Many genes in the ‘Carbon fixation in photosynthetic organisms’ pathway in salt stress and alkali stress were down-regulated. Metabolome showed that 431 and 378 differentially accumulated metabolites (DAMs) were identified in salt stress and alkali stress, respectively. L-Glutamic acid and 5-Aminolevulinate involved in chlorophyll synthesis decreased under salt stress and alkali stress. The abundance of 19 DAMs under salt stress related to photosynthesis decreased. The abundance of 16 organic acids in salt stress and 22 in alkali stress increased respectively. Conclusions: Our findings suggested that alkali stress had more adverse effects on grapevine leaves, chloroplast structure, ion balance, and photosynthesis than salt stress. Transcriptional and metabolic profiling showed that there were significant differences in the effects of salt stress and alkali stress on the expression of key genes and the abundance of pivotal metabolites in grapevine plants.

Water resources are scarce in the Northeastern Loess Plateau, and water cellar water (WCW) is a vital water resource available in the vast rural areas of the region. The quality of WCW was assessed by principal component analysis (PCA) and Nemerow’s pollution index (NPI) for different rainfall catchment areas, depths, and storage times. Eleven indicators were measured, including pH, electrical conductivity (EC), F⁻, Cl⁻, NO₃⁻, SO₄²⁻, Na⁺, NH₄⁺, Ca²⁺, Mg²⁺, and K⁺. The results show that the tap water quality in the rural areas of the Northeastern Loess Plateau is above the second level and meets the drinking water standard (DWS), which is similar to the tap water quality in the region. The main component score of water quality from tile roof + cement ground (I) is 0.32, and the Nemero index is 0.41; the principal component score of water quality from cement ground (I) is 0.45, and the Nemero index is 0.29; the principal component score of water quality from trampled land (I) is 0.59, and the Nemero index is 0.44; the principal component score of water quality from tile roof + trampled land (II) is 1.87, and the Nemero index is 1.10. The rainwater harvesting catchment area of tile roof + cement ground (I) ensured the highest water quality, followed by cement ground (I), trampled ground (I), and tile roof + trampled ground (II). The water quality of the catchment area for artificially collected rainwater (roof tile surface, cement ground, etc.) was better than that of the original soil (trampled ground). The highest water quality was found at a storage time of 1 year (I), followed by 2.5 years (I), and 2 months (II). A depth of 4 m (I) contributed to the highest water quality, followed by 2 m (II), 3 m (II), and 1 m (II). Water quality improved with the increasing depth of WCW. The rainfall and WCW in the area were weakly alkaline, and the groundwater was contaminated with NO₃⁻. PCA’s water quality assessment results were similar to the NPI method, indicating that both methods can be used in combination for unconventional water quality assessment.