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(searched for: doi:(10.33988/*))
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Alper Karagöz, Hidayet Tutun, Tutku Arslantaş, Özlem Altintaş, Nadir Koçak, Levent Altintaş
Ankara Üniversitesi Veteriner Fakültesi Dergisi; doi:10.33988/auvfd.775884

Abstract:
A novel coronavirus (SARS-CoV-2) outbreak, responsible for a pneumonia-associated respiratory disorder (COVID-19), has started in early December 2019 in Wuhan, China, and has rapidly spread around the world. Rapid and accurate diagnostic testing plays a crucial role in tackling the COVID-19 pandemic. In this study, it was aimed to compare 5 primer sets designed to amplify different regions for the detection of SARS-CoV-2 and to perform sequence analysis. Conventional RT-PCR was carried out using primers targeting different regions of the virus genome including ORF1ab, Envelope (E), RNA-dependent RNA polymerase (RdRp), Spike (S) and Nucleocapsid (N) genes for the diagnosis of COVID-19. DNA sequence of ORF1ab gene from each sample were compared with the DNA sequence data of SARS-CoV-2 stored in the GenBank and ORF1ab phylogenetic tree was constructed. The amplicon sizes of ORF1ab, S, E, N and RdRp genes were 588 bp, 440 bp, 145 bp, 323 bp and 196 bp, respectively. The 2019-nCoV RNA was detected from 74% of total samples from RdRp gene, 87% for N gene, 74% for S gene, 61% for E gene and 82% for ORF1ab region. The ORF1ab sequences of SARS-CoV-2 from 82 patients were had 100% identity to the sequence of Wuhan isolate and among themselves. The phylogenetic analysis revealed that all isolates formed a cluster. The results of this study suggest that the N region is the best for SARS-CoV-2 identification.
Evren Erdem, Fatma Tülin Özbaşer, Sciprofile linkFatih Atasoy
Ankara Üniversitesi Veteriner Fakültesi Dergisi; doi:10.33988/auvfd.644290

Abstract:
The aim of this study was to determine the reproductive characteristics of Saanen goats of Australian origin using an estrus synchronization programme with hormones (MPA and PMSG) in two different mating period (Period I and Period II) and to investigate the survival rates and growth performances of kids. According to the results of the research, it was determined that pregnancy rate and litter size were higher in mating period I (87% and 2.16) compared to mating period II (80.5% and 2.03), and it is hypothesized that this situation is related to the length of dry period of the goats. Although the mortality rate (P˂0.001), and body weight (P˂0.05) at birth were affected by kidding season, the survival rate and their body weights of the Saanen-kids at days 30, 60, 90 and 120 were not affected by the kidding season (P>0.05). These results indicate that the kidding season should be an environmental factor to be taken into account when planning the production. In addition, if the estrus synchronization program will be implemented in Saanen goats, it is important to optimize the care and management conditions, especially during the kidding season, due to the high multiple birth types.
Yavuz Yener, Sciprofile linkSakine Yalçin, Irfan Çolpan
Ankara Üniversitesi Veteriner Fakültesi Dergisi; doi:10.33988/auvfd.716897

Abstract:
The aim of the research was to determine the effects of red ginseng root powder on performance, immune system, caecal microbial population and some blood parameters in broilers. A total of 224 daily Ross 308 male broiler chicks were divided into one control group and three experimental groups each containing 56 chicks. Red ginseng root powder was added to the diets of groups at the level of 0, 75, 150 and 225 mg/kg, respectively. The experimental period was 38 days. There were no differences in growth performance. Addition of 225 mg/kg of the red ginseng root powder increased the relative weight percentages of spleen and gizzard (P
Ferruh Dinçer, Tamay Başağaç Gül
Ankara Üniversitesi Veteriner Fakültesi Dergisi; doi:10.33988/auvfd.747062

The publisher has not yet granted permission to display this abstract.
Tuğçe Tinmaz, Baran Çelik, Barış Halaç, Arzu Funda Bağcigil
Ankara Üniversitesi Veteriner Fakültesi Dergisi; doi:10.33988/auvfd.666779

Abstract:
In this study, it was aimed to investigate the presence of Pasteurella multocida in the swab samples taken from the oral cavity of cats, and to determine the capsular type and antimicrobial susceptibility of the isolates. For this purpose, swab samples taken from 300 cats were inoculated onto Knight's selective enriched medium and blood agar for isolation of P. multocida. Following after capsular typing of the isolates by PCR, the susceptibilities of the isolates to ceftiofur, clindamycin, erythromycin, enrofloxacin, amoxicillin+clavulanic acid, trimethoprim/ sulfamethoxazole, tetracycline, and chloramphenicol were examined by the disk diffusion method. The relationships between oral colonization and various physiological and behavioral variables were evaluated, statistically. P. multocida was isolated from 48 (16%) of the samples and all strains were identified as capsular type A. While all of the isolates were resistant to clindamycin and sensitive to enrofloxacin, 8,33% tetracycline resistance was also remarkable. Multiple antibiotic resistance (MDR) was detected in 27% of the isolates. The colonization of P. multocida was found to be statistically significant in cats under the age of 1 and in cats living outdoors. The importance of antibiotic resistance observed in bacteria with a zoonotic character such as P. multocida should not be ignored since it poses a threat to public health. It was concluded that, in this study determination of tetracycline resistance, which has started to be reported in feline Pasterurella multocida isolates in recent years, and detection of multiple antibiotic resistance in 27% of isolates, was extremely important for public health.
Ayhan Atasever, Duygu Yaman Gram, Görkem Ekebaş
Ankara Üniversitesi Veteriner Fakültesi Dergisi, Volume 67, pp 427-430; doi:10.33988/auvfd.566781

Abstract:
A five-year-old male rabbit was presented with the request of necropsy. Post mortem examination revealed a mass in the cranial mediastinum. Histopathologically, it was seen that the mass consisted of a limited mesenchymal tissue with medium-sized mature lymphocytes and a large proportion (approximately 90%) of lymphoblast-like round cell infiltration. While tumour cells were stained positively for CD3, they were negative for CD79a. Pan-Cytokeratin was localized in small number of epithelial cells in the background. Histopathological and immunohistochemical evaluation showed that the case was compatible with thymic lymphoma and it was the first case reported in rabbits in Turkey.
Yağmur Nil Demirel, Şebnem Pamuk, Sciprofile linkZeki Gürler
Ankara Üniversitesi Veteriner Fakültesi Dergisi, Volume 67, pp 343-347; doi:10.33988/auvfd.601699

Abstract:
Biogenic amines (BAs) are formed by decarboxylation of amino acids, amination and transamination of aldehyde and ketone groups. The excess of BAs is harmful to human health. BAs play a significant role in determining the shelf life and quality of foods. Released type and amount of BAs depend on factors such as the quality of the raw material, the diversity of natural microbiota, processing and storage conditions. In fish, the release of BAs is affected primarily from microbial growth as well as other reasons and may cause poisoning. It was aimed to determine the possibility of histidine decarboxylase activity gene (hdc) and tyrosine decarbocylase activity gene (tyrdc) in lactic acid bacteria (LAB) which were isolated from sea bream and sea bass. A total of 18 Gram positive-catalase negative LAB was isolated from 84 fish samples from 14 different fish markets. It was found that 12 out of 18 LAB (67%) isolates showed negative histidine and tyrosine decarboxylase activities. While 2 out of 6 (11%) LAB isolates were determined positive only tyrosine decarboxylase and 4 of them (22%) were positive for histidine and tyrosine decarboxylase. As a result of the Polymerase Chain Reaction (PCR), 9 out of 12 LAB isolates (75%) were found to have histidine decarboxylase activity gene. As a result, the prevalence of histidine decarboxylase activity gene in the LAB has detected more extensive than tyrosine decarboxylase activity gene. Increasing the studies examining the presence of aminobiogenic microorganisms in fish is important for the protection of public health.
Seyda Cengiz, Mehmet Cemal Adigüzel
Ankara Üniversitesi Veteriner Fakültesi Dergisi; doi:10.33988/auvfd.640990

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