(searched for: doi:10.33084/*)
Borneo Journal of Pharmacy, Volume 2, pp 108-113; doi:10.33084/bjop.v2i2.928
Abstract: Pineapple peel (Ananas comosus (L.) Merr.) is waste from the pineapple fruit. The purpose of this study was to determine the content of pineapple peel extract and to antibacterial activity in various concentrations effective in inhibiting the growth of Propionibacterium acnes. The pineapple peel extract is made by using the extraction method in the form of maceration. The method used in the inhibitory test using the three replication samples in each treatment group. The sample consisted of 10 treatment groups ie pineapple peel extract concentration 12.5%, 25%, 37.5%, 50%, 62.5%, 75%, 87.5%, and 100%, as well as positive and negative control. The results obtained from pineapple peel screening contain flavonoid and saponin compounds. The data analysis shows that pineapple peel extract concentrations of 50%, 62.5%, 75%, 87.5%, and 100% had inhibitory zones with the medium-strong category while clindamycin as the control has a strong inhibition zone. The conclusion of this research proves that pineapple peel extract has antibacterial power to P. acnes with effective concentration is 100% with the strong category.
Borneo Journal of Pharmacy, Volume 2, pp 114-118; doi:10.33084/bjop.v2i2.1070
Abstract: Tandui (Mangifera rufocostata Kosterm.) is a typical plant from South Kalimantan which belongs to the genus of Mangifera. Several species of Mangifera are known to have antioxidant activity. This study aimed to determine the antioxidant activity of ethanol extract of Tandui leaves. Tandui leaves that were obtained from the maceration method used 70% ethanol. Antioxidant activity was conducted quantitative using the DPPH (2,2-diphenyl-1-picrylhydrazil) method. The result of the antioxidant activity of Ethanol extract of Tandui leaves quantitatively obtained IC50 value was 60.7042 �g/mL. The ethanol extract of Tandui leaves has strong antioxidant activity
Borneo Journal of Pharmacy, Volume 2, pp 35-40; doi:10.33084/bjop.v2i2.868
Abstract: The previous study reported that Etlingera elatior (Wualae) has activity as immunostimulant with dose at 300 mg/kg BW and 400 mg/kg BW. Formulating natural product into effervescent granule (GE) can increase practicality and interest to consume traditional medicine. This study aims to investigate the immunostimulant activity of Wualae ethanolic extract in the effervescent granule. Wualae was macerated with ethanol then formulated into effervescent granule at dose 300 mg/kgBW and 400 mg/kgBW. Formulations were evaluated. Animals were divided into 4 groups and treated with: group I (positive control); group II (negative control); group III (GE dose at 300mg/kgBW); and group IV (GE at dose 400mg/kgBW) for 7 days and at days 8, groups were infected by Staphylococcus aureus intraperitoneally. Immunostimulant activity was measured by calculating the Specific Phagocytic Activity (SPA) of macrophage. Data collected from the evaluation and measurement of the SPA then analyzed using SPSS. Results from the evaluation were considered good except the moisture content of effervescent granule. Results from SPA was found that effervescent granule at dose 300 mg/kgBW and at dose 400 mg/kgBW have 26.33% and 32.5% consecutively with significance 0.02 (p
Borneo Journal of Pharmacy, Volume 2, pp 55-62; doi:10.33084/bjop.v2i2.898
Abstract: Jualing (Micromelum minutum Wight & Arn.) is a plant from South Kalimantan that has the potential to be developed as natural medicine. This study aims to establish standardization which includes specific and non-specific parameters of M. minutum leaf simplicia and extracts from three growing locations, namely Banua Botanical Garden, Sultan Adam Forest Park, and Forest Areas with Specific Objects of Rantau, South Kalimantan. The method of setting standardization parameters refers to the Indonesian Herbal Pharmacopoeia and General Standard Extracts Parameters. Organoleptic standardization of simplicia is brownish-green, has a distinctive odor, has a bitter and slightly spicy taste. Microscopic observations showed the presence of stomata, cell walls, cytoplasm, calcium oxalate crystals, upper epidermis, palisade tissue, spongy tissue, cortex, xylem, phloem, lower epidermis, and trichomes. Water content test showed the results of 21.9-22.07%; ethanol extract content of 12.87-13.17%; drying losses 4.64-4.84%; total ash content of 6.04-6.14%; acid insoluble ash content 1.13-1.19%; Pb levels of 0.022-0.025 mg/kg; Cd levels of 0.017-0.020 mg/kg; and Hg levels
Borneo Journal of Pharmacy, Volume 2, pp 119-124; doi:10.33084/bjop.v2i2.875
Abstract: Rational drug use (RDU) is an indicator to evaluates a treatment given to patients, like proper medication, precise diagnosis, precise dosing. Community Health Center (CHC) has the risk of irrational drug use. In this study, researchers selected N and BL CHC, aiming to evaluate the prescribing indicators based on three diseases which are non-pneumonia acute respiratory infection (ARI), non-specific diarrhea and myalgia, and the facility indicators with DOEN (list of essential national medicines) availability and 20 mandatory drugs. This research was a descriptive-analytical study with cross-sectional methods where the data retrieval of the prescribing is taken from January to December 2018. The study used the normality test and homogeneity test before independent sample T-test, from the third outcome of the test, the N and BL CHC could be said to differ significantly of RDU. It can be concluded that rational drug use is reviewed from a prescribing indicator based on disease and facility indicator. The results of RDU are rational in N CHC, in contrast with BL CHC which is not rational with the results of the RDU in N CHC is 101.44% and BL CHC is 89.81%. The results of N CHC is better than BL CHC, which both CHCs have fulfilled the target of the government, for 68% in 2018. In both CHCs for the facility indicator, there are a DOEN and 20 essential medicines.
Borneo Journal of Pharmacy, Volume 2, pp 63-70; doi:10.33084/bjop.v2i2.981
Abstract: Acne is a pile of oil glands in the skin that are actively being clogged by dirt and infection because of Propionibacterium acnes, Staphylococcus aureus, and Staphylococcus epidermidis. The treatment for acne can use herbal ingredients, one of which is Soursop (Annona muricata L.) leaves ethanolic extract. Annona muricata folium ethanolic extract is known to have alkaloid, flavonoid and polyphenol compounds which can inhibit the growth of P. acnes, S. epidermidis, and S. aureus. Suitable dosage forms for anti-acne preparations are gels because the gel contains much water so that it provides a cooling effect on the skin due to inflammation. The purpose of this study was to determine the impact of carbopol 940 and Propylene glycol on the physical characteristics of the gel preparation and determine the optimum formula of the gel preparation using factorial design software 22 so that the gel preparation was made in four formulas. The data obtained were then analyzed using Design-Expert� and ANOVA software with a confidence level of 95%, and the determination of the optimum formula based on the optimum superimposed contour plot area. The results of the study using a factorial design approach are known that Propylene glycol has an effect on increasing the spreadability and pH while carbopol 940 has a more significant impact on the increase in adhesion and viscosity, and the optimum area of ??the composition of carbopol 940 1.536% and propylene glycol 5% was obtained.
Borneo Journal of Pharmacy, Volume 2, pp 87-93; doi:10.33084/bjop.v2i2.901
Abstract: Food allergies are one of the most common allergies in Indonesian society. Generally, when children aged 5-6 years food allergies will disappear, except peanut allergies and allergies to seafood, such as fish, shellfish and crustaceans. This study aims to determine the pattern of separation of allergen proteins in shrimp using anion exchange column chromatography method and identify allergen proteins in shrimp using the sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) method. Protein extraction from shrimp using Phosphate buffer saline (PBS) pH 7.2 and centrifuged at 10,000 rpm for 10 min at 4�C. Protein separation was carried out by anion-exchange column chromatography method, and the fraction obtained was measured at 280nm wavelength. The highest yield at absorbance was identified by using SDS-PAGE. Polyacrylamide gel electrophoresis was used to determine the protein profile and molecular weight of shrimp extract. Coloring of protein bands using silver staining. Data were analyzed descriptively based on the migration value of the sample protein bands compared to the marker protein band (Rf). The results of protein allergen profile analysis on shrimp using SDS-PAGE showed that the shrimp contained a protein band with a molecular weight of 37.77 kDa for cooked shrimp and 37.03 kDa for fresh shrimp.
Borneo Journal of Pharmacy, Volume 2, pp 125-132; doi:10.33084/bjop.v2i2.1044
Abstract: The purpose of this study is to assess the rationality of the use of antibiotics from inpatients in one hospital in Cimahi, West Java. The research about rationality analysis of antibiotics at pediatric inpatient in one of the hospitals in Cimahi was done retrospectively use descriptive analysis design. The data source was the medical record of a patient who treated at the children's inpatient ward from January through March 2017. The result shows that 39% of infected patients were children under one-year-old. Three significant cases of diseases that occurred in the period were bronchopneumonia, typhoid fever, and typhoid fever+bronchopneumonia. Antibiotics therapy was given as single therapy as well as combination, where cefotaxime (51%) and ceftriaxone (42%) were the most antibiotics widely used as single therapy. Antibiotic use at children inpatient ward during the period January � March 2017 on bronchopneumonia, typhoid fever, and typhoid fever + bronchopneumonia patients was not 100% rational referring to criteria: correct diagnosis, correct indication, the correct drug of choice, correct dosage, correct method, and correct time interval of drug administration.
Borneo Journal of Pharmacy, Volume 2, pp 71-81; doi:10.33084/bjop.v2i2.836
Abstract: Ebola viral disease (EVD) is a deadly infectious hemorrhagic viral fever caused by the Ebola virus with a high mortality rate. Until date, there is no effective drug or vaccination available to combat this condition. This study focuses on designing an effective antiviral drug for Ebola viral disease targeting viral protein 30 (VP30) of Ebola virus, highly required for transcription initiation. The lead molecules were screened for Lipinski rule of five, ADMET study following which molecular docking and bioactivity prediction was carried out. The compounds with the least binding energy were analyzed using interaction software. The results revealed that 6-Hydroxyluteolin and (-)-Arctigenin represent active lead compounds that inhibit the activity of VP30 protein and exhibits efficient pharmacokinetics. Both these compounds are plant-derived flavonoids and possess no known adverse effects on human health. In addition, they bind strongly to the predicted binding site centered on Lys180, suggesting that these two lead molecules can be imperative in designing a potential drug for EVD.
Borneo Journal of Pharmacy, Volume 2, pp 48-54; doi:10.33084/bjop.v2i2.1020
Abstract: Hydrargyrum (familiar as mercury) is a persistent transition metal element. They are known as bio-accumulative properties that are dangerous to the environment and has serious damaging effects on biota, including genetic changes or mutagenesis. Moreover, it can accumulate indirectly in the human body due to the consumption of products that have contaminated. Whereas, ellagic acid has reported as one of 13 flavonoid compounds that have potential as an anti-mutagen and anti-cancer in the walnuts seeds. This study was aimed to ensure the potential extent of ellagic acid extracts in walnut seeds to reducing and repairing the genotoxicity effects of mercury. Therefore, using the experimental method, this study was divided into several test treatments. Two controls treatment as a comparison of the presence of micronucleus with group tests that given a 20 ppm mercury dosage. Then continued with the addition of walnut seed extract with various dosage (10, 15, and 20 ppm) by oral injection continuously for two weeks as a recovery process to reducing and repairing the genotoxicity effect of mercury. The significant results showed that there are differences between pre and post-treatment. It indicated that the extract has the potential for improving and reducing the genotoxicity effect of mercury. The data were evaluated and obtained the total micronucleus from the smear of peripheral blood of mice by comparing the total micronucleus before and after giving of walnut extract.