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(searched for: HMGB1-A-Potential-Therapeutic-Target-for-Non-Small-Cell-Lung-Cancer)
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T Flieswasser, J Van Loenhout, K Zwaenepoel, C Rolfo, F Lardon, E Smits, J Jacobs, P Pauwels
Abstracts of the 25th Biennial Congress of the European Association for Cancer Research, Amsterdam, The Netherlands, 30 June – 3 July 2018, Volume 3; doi:10.1136/esmoopen-2018-eacr25.940

The publisher has not yet granted permission to display this abstract.
Letters in Health and Biological Sciences, Volume 1, pp 1-2; doi:10.15436/2475-6245.16.008

Peng Xiao, Sciprofile linkWen-Liang Liu
International journal of clinical and experimental pathology, Volume 8, pp 10800-10807

Abstract: Background: microRNAs (miRNAs) play
a
significant role in
cancer
development and progression by regulating the expression of oncogenes or tumor suppressor genes. Previous study using microarrays demonstrated that miR-142-3p was downregulated in patients with
Non-small-cell
lung
carcinoma (NSCLC). However, the functional role of miR-142-3p in NSCLC is still unclear. Material and method: Real-time quantitative PCR was applied to evaluate the expression level of miRNA-142-3p in NSCLC and normal samples. The
cell
proliferation of NSCLC
cells
was analyzed by MTT and colony formation assay after miR-142-3p transfection. Luciferase activities assay, cotransfection and Western blot were used to reveal that the predicted
target
genes of miR-125b were direct and specific. Results: In this study, we demonstrate miR-142-3p was downregulated in NSCLC tissues and
cell
lines. We demonstrated that the overexpression of miR-142-3p inhibits NSCLC
cell
proliferation and induced
cell
apoptosis. Furthermore, we demonstrate
HMGB
1
was
a
directly
target
of miR-142-3p in NSCLC
cells
, and confirmed the
target
specificity between miR-142-3p and the
HMGB
1
3’-untranslated region by luciferase reporter assay. Conclusions: These results suggest that miR-142-3p may be
a
tumor suppressor through the downregulation of
HMGB
1
in NSCLC. miR-142-3p may be
a
tumor suppressor and
a
potential
therapeutic
agent
for
patients with NSCLC.
Cailian Zhang, Shengli Ge, Cailian Hu, Ning Yang, J. Zhang
Acta Biochimica et Biophysica Sinica, Volume 45, pp 1055-1061; doi:10.1093/abbs/gmt109

Abstract: MicroRNAs (miRNAs) function as negative regulators of gene expression involved in
cancer
metastasis. The aim of this study is to investigate the
potential
roles of miR-218 in
non-small
cell
lung
cancer
and validate its regulation mechanism. Functional studies showed that miR-218 overexpression inhibited
cell
migration and invasion, but had no effect on
cell
viability. Enhanced green fluorescent protein reporter assay, real-time polymerase chain reaction and western blot analysis confirmed that miR-218 suppressed the expression of high mobility group box-
1
(
HMGB
1
) by directly
targeting
its 3'-untranslated region. Accordingly, silencing of
HMGB
1
accorded with the effects of miR-218 on
cell
migration and invasion, and overexpression of
HMGB
1
can restore
cell
migration and invasion which were reduced by miR-218. In conclusion, these findings demonstrate that miR-218 functions as
a
tumor suppressor in
lung
cancer
. Furthermore, miR-218 may act as
a
potential
therapeutic
biomarker
for
metastatic
lung
cancer
patients.
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