Label-Free Myoglobin Biosensor Based on Pure and Copper-Doped Titanium Dioxide Nanomaterials
Open Access
- 8 December 2022
- journal article
- research article
- Published by MDPI AG in Biosensors
- Vol. 12 (12), 1151
- https://doi.org/10.3390/bios12121151
Abstract
In this study, using pure and copper-doped titanium dioxide (Cu-TiO2) nanostructures as the base matrix, enzyme-less label free myoglobin detection to identify acute myocardial infarction was performed and presented. The Cu-TiO2 nanomaterials were prepared using facile sol–gel method. In order to comprehend the morphologies, compositions, structural, optical, and electrochemical characteristics, the pure and Cu-TiO2 nanomaterials were investigated by several techniques which clearly revealed good crystallinity and high purity. To fabricate the enzyme-less label free biosensor, thick films of synthesized nanomaterials were applied to the surface of a pre-fabricated gold screen-printed electrode (Au-SPE), which serves as a working electrode to construct the myoglobin (Mb) biosensors. The interference study of the fabricated biosensor was also carried out with human serum albumin (HSA) and cytochrome c (cyt-c). Interestingly, the Cu-doped TiO2 nanomaterial-based Mb biosensor displayed a higher sensitivity of 61.51 µAcm−2/nM and a lower detection limit of 14 pM with a response time of less than 10 ms.Keywords
Funding Information
- Deanship of Scientific Research at Najran University, Najran, Kingdom of Saudi Arabia (NU/NRP/SERC/11/1)
This publication has 55 references indexed in Scilit:
- Disposable amperometric magnetoimmunosensor for the sensitive detection of the cardiac biomarker amino-terminal pro-B-type natriuretic peptide in human serumAnalytica Chimica Acta, 2013
- Sensitive and reproducible detection of cardiac troponin I in human plasma using a surface acoustic wave immunosensorSensors and Actuators B: Chemical, 2013
- Label-free electrochemiluminescence immunosensor for cardiac troponin I using luminol functionalized gold nanoparticles as a sensing platformThe Analyst, 2013
- Employing denaturation for rapid electrochemical detection of myoglobin using TiO2 nanotubesJournal of Materials Chemistry B, 2013
- Electrochemical sensors for hemoglobin and myoglobin detection based on methylene blue-multiwalled carbon nanotubes nanohybrid-modified glassy carbon electrodeElectrochimica Acta, 2011
- Electrochemical Immunosensor Based on Metal Nanoparticles for Cardiac Myoglobin Detection in Human Blood PlasmaElectroanalysis, 2011
- Antigen Peptide-Based Immunosensors for Rapid Detection of Antibodies and AntigensAnalytical Chemistry, 2009
- New Nanostructured TiO2 for Direct Electrochemistry and Glucose Sensor ApplicationsAdvanced Functional Materials, 2008
- National Academy of Clinical Biochemistry Laboratory Medicine Practice Guidelines: Clinical Characteristics and Utilization of Biochemical Markers in Acute Coronary SyndromesClinical Chemistry, 2007
- Sensitive and real-time fiber-optic-based surface plasmon resonance sensors for myoglobin and cardiac troponin ITalanta, 2004