Gossypin’in Fare Mekano-Biyoregülatör Modeli Deneysel Femur Kırıklarında Kırık İyileşmesi Üzerine Etkileri

Abstract

This paper aimed to research the possible effects of gossypin in a mechano-bio-regulatory mouse model for bone fracture healing. A total of 28 male Mus musculus BALB/c mice were randomly selected. Four groups were created. Each group consisted of 7 mice. The Control group was called Group 1. Group 2 was a femur fractured group of mice without any medication. Group 3 was the dose group of 5 mg/kg gossypin to mouse with femur fractures. Group 4 was the dose group of 10 mg/kg gossypin to mouse with femur fractures. An open fracture model was created in the right femur of the animals in Groups 2, 3, and 4. The radiological views for all groups were taken on postoperative 1st day, 6th week, and 12th week. All samples were obtained, collected, and prepared for biomechanical features, histopathological examinations, biochemical tests, and PCR tests. In terms of radiological and histological results, gossypin showed a significant difference depending on the dose in Group 4 to Group 3. Gossypin had sufficient antioxidant and anti-inflammatory effects in mice with femur fracture in bone healing in Groups 3 and 4. Biomechanical tests showed enough hardness levels and high thresholds in braking forces.