Copper nanocluster-Ru(dcbpy)32+ as a cathodic ECL-RET probe combined with 3D bipedal DNA walker amplification for bioanalysis
- 29 November 2022
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 148 (1), 114-119
- https://doi.org/10.1039/d2an01321e
Abstract
In this study, a cathodic intra-molecular electrochemiluminescence resonance energy transfer (ECL-RET) probe was exquisitely designed via the integration of an ECL donor (Cu NCs) with an acceptor (Ru(dcbpy)3 2+), and further employed the 3D bipedal DNA walker amplification strategy to monitor the platelet-derived growth factor BB (PDGF-BB). Specifically, blue emission Cu NCs with low consumption, biocompatibility and numerous resources, act as well-overlapped donors and significantly improve the ECL efficiency of Ru(dcbpy)3 2+. More impressively, the intra-molecular ECL-RET of Cu NC-Ru endowed a better and more stable ECL signal by reducing the electron-transfer distance and decreasing the energy loss. Furthermore, 3D bipedal DNA walker amplification was employed to efficiently convert the target PDGF-BB into numerous DNA strands, achieving sensitive target amplification. By virtue of such design, the constructed aptasensor exhibited a sensitive and selective assay for PDGF-BB with a detection range from 0.01 pM to 10 nM and a detection limit of 3.3 fM. The intramolecular ECL-RET and 3D bipedal DNA walker amplification strategy designed in this study will provide valuable insight into promising ultrasensitive ECL bioanalysis.Keywords
Funding Information
- Natural Science Foundation of Shaanxi Province (2018JM2058, 2018JM5180)
- National Natural Science Foundation of China (21973073, 21973074 and 21727805)
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