Beneficial Role of Nigella Sativa Extracted Oil on the Hepato-toxic Effect of Ceftriaxone in Adult Male Albino Rat (Histological and Immuno-histochemical Study)

Abstract

Ceftriaxone is a broad-spectrum cephalosporin with a hepatotoxic effect. Nigella sativa (Ns) has potent antioxidant effects, so it has a promising medicinal application. Aim of the work: to study the possible protective role of Nigella Sativa Extracted Oil against the Hepato-toxic Effect of Ceftriaxone in adult male albino rat. Material and methods: thirty two adult male albino rats had been divided into four groups with 8 rats allocated in each group: Group 1 (control group), Group 2 (Ns oil administrated): received Nigella sativa (NS) oil 2 ml/kg by gastric tube daily for 4 weeks, Group 3 (ceftriaxone treated): received ceftriaxone (360 mg/kg) (I.P) daily for 4 weeks and Group 4 (Ns oil and ceftriaxone treated group) .At the last day of the experiment, the liver specimens of each animal were dissected carefully and were processed for light-microscopic examination. Results: Ceftriaxone treated group revealed elevated level of liver enzymes that were relatively lower in ceftriaxone and NsL group. Histological examination of the liver of ceftriaxone treated group showed dilated congested central vein and sinusoids with hydropic degeneration of hepatocytes, while portal triad revealed dilated congested portal vein and dilated bile ducts surrounded by inflammatory cells. Additionally, abundant deposition of collagen was detected and increased expression of TNFα immune staining within hepatocytes and in lining of sinusoids. All these histological alterations were less marked in group (4) which received NSL in combination with ceftriaxone. Conclusion: NsL has a protective effect against liver injury resulted from ceftriaxone injection due to its antioxidant activities.