A Brief Review of How to Construct an Enzyme-Based H2O2 Sensor Involved in Nanomaterials
Open Access
- 1 January 2021
- journal article
- review article
- Published by Scientific Research Publishing, Inc. in Advances in Nanoparticles
- Vol. 10 (01), 1-25
- https://doi.org/10.4236/anp.2021.101001
Abstract
This article briefly reviews how to construct an enzyme based hydrogen peroxide sensor involving nanomaterials, which has the advantages of high efficiency, good sensitivity and selectivity, fast response time and an extended range of linearity with lower detection limit. Glucose biosensor is constructed by immobilizing glucose oxidase enzyme on the polycarbonate membrane and the protective cover is then filled with a physiological phosphate buffer, pH 7.4. The novel blocking hydrophobic membrane which is only permeable to hydrogen peroxide is used to eliminate electrochemical interferences. This constructed enzyme based H2O2 biosensor is miniaturized by the involvement of nanomaterials like carbon nanotubes, platinum nanoparticles and silver nanoparticles and it can achieve the effective microscopic detection of glucose. The introduction of nanomaterials including some pure metals (Ag, Au, Pd, Ni, Pt, and Cu), metal oxide (ZnO and TiO2), bimetallic (Au/Ag and Au/Pt) and carbon (nanotubes and graphene) nanomaterials in the construction of the enzyme based H2O2 biosensor improves its sensitivity and performance by enhancing the enzymatic activity, and allows the introduction of many new signal transduction technologies in biosensors. This review article summarizes the working principles of glucose oxidase based hydrogen peroxide sensor, importance of involving nanomaterials in biosensor manufacturing, basic characteristics and components of a biosensor, generations glucose biosensors, procedure of making hydrogen peroxide based biosensor, synthesis of nanomaterials involved in hydrogen peroxide biosensor, and finally some examples of nanomaterials which intervene in hydrogen peroxide biosensor.Keywords
This publication has 56 references indexed in Scilit:
- Cysteine-Mediated Aggregation of Au Nanoparticles: The Development of a H2O2 Sensor and Oxidase-Based BiosensorsACS Nano, 2013
- Graphene wrapped Cu2O nanocubes: Non-enzymatic electrochemical sensors for the detection of glucose and hydrogen peroxide with enhanced stabilityBiosensors and Bioelectronics, 2013
- ZnO/Cu Nanocomposite: A Platform for Direct Electrochemistry of Enzymes and Biosensing ApplicationsLangmuir, 2012
- Hydrogen peroxide biosensor based on gold nanoparticles/thionine/gold nanoparticles/multi-walled carbon nanotubes–chitosans composite film-modified electrodeApplied Surface Science, 2012
- A novel glucose biosensor based on direct electrochemistry of glucose oxidase incorporated in biomediated gold nanoparticles–carbon nanotubes composite filmSensors and Actuators B: Chemical, 2011
- A novel H2O2 amperometric biosensor based on gold nanoparticles/self-doped polyaniline nanofibersBioelectrochemistry, 2011
- Characterising a technology development at the stage of early emerging applications: nanomaterial-enhanced biosensorsTechnology Analysis & Strategic Management, 2011
- New‐generation nano‐engineered biosensors, enabling nanotechnologies and nanomaterialsSensor Review, 2008
- Hydrogen peroxide sensor based on horseradish peroxidase-labeled Au colloids immobilized on gold electrode surface by cysteamine monolayerAnalytica Chimica Acta, 1999
- Optical sensor for hydrogen peroxideMicrochimica Acta, 1989