In order to learn the oil content of the fruits of four newly selected hybrid seabuckthorn clones, after sampling the fruits of hybrid seabuckthorn in Heilongjiang, Liaoning, Gansu, Qinghai and Xin-jiang from 2016 to 2018, Soxhlet extraction method was used to analyze the oil content of dry whole fruit, dry pulp and dry seed. The average oil content of dry whole fruit of hybrid seabuckthorn was 15.62%, of which “zaciyou 54” was the smallest, only 13.16%, and “zaciyou 01” was the largest, up to 21.24%. In the dried whole fruit, the oil content of dried pulp was 17.59% ± 5.81%, which was 3.60 percentage points higher than 13.99% ± 6.63% of dry seeds. In the five experimental locali-ties, the oil content of dry whole fruit of hybrid seabuckthorn was the highest in Datong, Qinghai, up to 18.66% ± 3.86%, and the lowest in Emin, Xinjiang, only 13.35% ± 6.63%. For the test three years, the oil content of dried whole fruit of hybrid seabuckthorn was 9.50% ± 2.41% in 2016, 17.70% ± 4.64% in 2017 and 11.36% ± 2.27% in 2018, showing an obvious fluctuation of “small-large-small”. Although the oil content of hybrid seabuckthorn was 1.49% lower than that of introduced seabuckthorn, it was really high. In addition, as hybrid seabuckthorn had strong adaptability, industrial raw material forests with oil as the main goal should be established in the “Three North” and southwest of China for comprehensive development and utilization.