Preliminary investigation of Nauclea latifolia ripe fruits for antioxidant and antidiabetic activities
Open Access
- 10 September 2019
- journal article
- Published by ANSF Publications in Journal of Applied and Natural Science
- Vol. 11 (3), 718-723
- https://doi.org/10.31018/jans.v11i3.2153
Abstract
Fruits of Nauclea latifolia (Family Rubiaceae) have been used as food and medicinal plants. The ethnomedicinal reports indicated that it can be traditionally used for the treatment of dysentery, diarrhea, diabetes, malaria etc. The aim of this work was to evaluate the antioxidant, ?–amylase and ?–glucosidase activities of methanol extract of N. latifolia fruits at varying concentrations (20-100 µg/ml) using standard methods. The results of the DPPH and nitric oxide free radical scavenging capacity showed IC50 values 92.0µg/ml and 30.0µg/ml respectively indicating a good inhibitory capacity but lesser when compared to the standard, ascorbic acid which are < 10.0µg/ml and < 20.0µg/ml respectively. The analysis shows total phenolic contents of 147.9±3.35mg/g and ferric reducing antioxidant potential of 1604.1±9.20mg/g supporting the fact that phenolics are highly potent antioxidants. Based on the results obtained from ?–amylase and ?–glucosidase inhibition, the IC50 > 100µg/ml showing a lesser inhibitory activity when compared to the standard Acarbose with IC50 values of 50.1µg/ml and 44.0µg/ml respectively. The results clearly indicate that the methanol extract of N. latifolia has a moderate free radical scavenging activity resulting from various interaction between different components of the plant. It can be concluded that the fruits may provide natural source of bioactive compounds which is beneficial to human health and can be used as basis of folkloric remedies for diabetes.Keywords
This publication has 7 references indexed in Scilit:
- Antioxidant Activity and Total Phenolic and Flavonoid Content of Various Solvent Extracts fromIn VivoandIn VitroGrownTrifolium pratenseL. (Red Clover)BioMed Research International, 2015
- Antioxidant activity of methanol extracts of different parts of Lantana camaraAsian Pacific Journal of Tropical Biomedicine, 2012
- Phenolic compounds, antioxidant activity and in vitro inhibitory potential against key enzymes relevant for hyperglycemia and hypertension of commonly used medicinal plants, herbs and spices in Latin AmericaBioresource Technology, 2010
- Antioxidant and free radical scavenging activities of edible weedsAfrican Journal of Food, Agriculture, Nutrition and Development, 2009
- Antioxidant Capacity of Some Herbs/Spices from Cameroon: A Comparative Study of Two MethodsJournal of Agricultural and Food Chemistry, 2005
- Screening of Plant Extracts for Antioxidant Activity: a Comparative Study on Three Testing MethodsPhytochemical Analysis, 2002
- [2] Ferric reducing/antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentrationMethods in Enzymology, 1999