Analysis of Antibacterial Activity of Antibiotics to Anaerobes of Female Genital Tract in Vitro

Abstract

This study to investigate predominant anaerobic bacteria and their sensitivity spectrum to antibiotics in female patients with genital tract infections from the local and thus to provide scientific basis for the appropriate use of antibiotics. Anaerobic culture method was used to culture and isolate anaerobes in female patients with genital tract infections. Anaerobes were identified by using micro-biochemistry reacting technique and antibiotic-disc susceptibility test. Antibacterial activity of antibiotics to anaerobes was analyzed by means of Kirby-Bauer testing and broth micro-dilution method. The experiment results showed that 72 strains of obligate anaerobes were isolated from 103 samples, positive rate of 69.90%. The predominant anaerobic bacterial were Gram-negative non-spore bacteroides (28 strains, 38.89%) and anaerobic peptostreptococcus (18 strains, 25%). Sensitivity rate of anaerobic cocci, bacteroides and veillonella to metronidazole was 90.91%, 85.71% and 80% respectively. The drug resistance rates of the three isolated anaerobes were all higher than 60% to amikacin, erythromycin and clindamycin. Comparing the MIC50 and MIC90 values of metronidazole (MTZ), penicillin G (PCG) and lincomycin (LCM) on the isolated anaerobes, MTZ had the lowest MIC50 and MIC90 value. The study suggested that anaerobic infection has become a major pathogenic bacterium of infectious diseases in obstetrics and gynecology. Although MTZ has shown strong antibacterial activity in vitro, a few drug resistant strains appeared in the clinically isolated anaerobes and there is an upward trend of the MIC values of MTZ at different rates.