Abstract
Context: Antioxidants are helpful in increasing the blood circulation and thus help in hair growth as well as in the treatment of diseases. Alopecia is a dermatological disorder with psychosocial implications on patients with hair loss. The antioxidant property of plant and different herbs can be utilized in hair fall conditions or many diseases. Aim: The aim of this study is to develop an herbal hair growth promoting shampoo using Piper betle and Psidium guajava leaves extract due to their antioxidant property. Materials and Methods: Antioxidant activity of herbal shampoo formulation containing P. betle and P. guajava leaves extract was determined using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) method, 0.5 ml of each sample at various concentrations was added to 1.5 ml of DPPH (0.004%) solution and allowed to stand in the dark at room temperature for 20 min. The absorbance at 520 nm was recorded using UV spectroscopy which was compared with ascorbic acid taken as standard. Results: The results of the study demonstrated that formulation FC3 exhibited best antioxidant activity and formulation was stable for 2 months. The formulation FC3 shows better pH, foamability, dirt dispersion, and wettability. Conclusion: The results of this study suggested that herbal shampoo formulation containing P. betle and P. guajava leaves extract is best for hair growth promoter and many problems related to hairs.