Driving involve activities some components including drivers, vehicles, passenger, roads, and the surrounding environment that interact with each other. Based on the website Badan Intelegensi Negara (BIN), it is said that one of the main causes of traffic accidents is the diver’s negligence. Data of Polrestabes Bandung City for the February 2014 to February 2015 showed that the rate of accidents, especially personal cars, had fluctuated. The driver factor that is the cause of the accident is most likely due to fatigue. The risk of work fatigue is on of them is stress. The impact of stress can disrupt driving performance arising from negative moods. Aim of this study was to analyze the stress and performance levels of driver personal car using salivary alpha amylase and stroop test parameters. The results of the study for sleep adequate treatment and lack of sleep treatment before and after simulation on stress levels (p-value (sig.) > α, 0,174; 0,351 > 0,05) and performance levels (p-value (sig.) > α, 0,546; 0,904 > 0,05) showed a significant distinction. While for stress level (p-value (sig.) > α, 0,365 > 0,05) about adequate sleep and lack of sleep treatment before and after simulation showed a significant distinction and performance level (p-value (sig.) > α, 0,915 > 0,05) showed a significant distinction. The coefitient correlation between stress level and performance level are 0.4, which mean there is strong correlation between stress level and performance level.