Hepatic Gene Expression Changes in Rats Internally Exposed to Radioactive 56MnO2 Particles at Low Doses
Open Access
- 22 July 2021
- journal article
- research article
- Published by MDPI AG in Current Issues in Molecular Biology
- Vol. 43 (2), 758-766
- https://doi.org/10.3390/cimb43020055
Abstract
We have studied the biological effects of the internal exposure to radioactive manganese-56 dioxide (56MnO2), the major radioisotope dust found in soil after atomic bomb explosions. Our previous study of blood chemistry indicated a possible adverse effect of 56MnO2 on the liver. In the present study, we further examined the effects on the liver by determining changes in hepatic gene expressions. Male Wistar rats were exposed to 56MnO2 particles (three groups with the whole-body doses of 41, 90, and 100 mGy), stable MnO2 particles, or external 60Co γ-rays (2 Gy), and were examined together with the non-treated control group on postexposure day 3 and day 61. No histopathological changes were observed in the liver. The mRNA expression of a p53-related gene, the cyclin-dependent kinase inhibitor 1A, increased in 56MnO2 as well as in γ-ray irradiated groups on postexposure day 3 and day 61. The expression of a stress-responsive gene, nuclear factor κB, was also increased by 56MnO2 and γ-rays on postexposure day 3. However, the expression of cytokine genes (interleukin-6 or chemokine ligand 2) or fibrosis-related TGF-β/Smad genes (Tgfb1, Smad3, or Smad4) was not altered by the exposure. Our data demonstrated that the internal exposure to 56MnO2 particles at less than 0.1 Gy significantly affected the short-term gene expressions in the liver in a similar manner with 2 Gy of external γ-irradiation. These changes may be adaptive responses because no changes occurred in cytokine or TGF-β/Smad gene expressions.Keywords
Funding Information
- Japan Society for the Promotion of Science (19H01149, 19KK0266)
This publication has 31 references indexed in Scilit:
- Gene expression following ionising radiation: Identification of biomarkers for dose estimation and prediction of individual responseInternational Journal of Radiation Biology, 2010
- Identification of Rat Prostatic Secreted Proteins Using Mass Spectrometric Analysis and Androgen-Dependent mRNA ExpressionJournal of Andrology, 2009
- Changes in transcriptome after in vivo exposure to ionising radiation reveal a highly specialised liver responseInternational Journal of Radiation Biology, 2009
- Skin dose from neutron-activated soil for early entrants following the A-bomb detonation in Hiroshima: contribution from β and γ raysRadiation and Environmental Biophysics, 2008
- Gamma-ray exposure from neutron-induced radionuclides in soil in Hiroshima and Nagasaki based on DS02 calculationsRadiation and Environmental Biophysics, 2008
- Effect of Radiation on Gene Expression of Rat Liver Chemokines:In VivoandIn VitroStudiesRadiation Research, 2008
- Smad3 as a mediator of the fibrotic responseInternational Journal of Experimental Pathology, 2004
- Dose‐dependent biphasic induction and transcriptional activity of nuclear factor kappa B (NF‐κB) in EA.hy.926 endothelial cells after low‐dose X‐irradiationInternational Journal of Radiation Biology, 2004
- Regulation of the G2/M transition by p53Oncogene, 2001
- Early alteration in TGF-β mRNA expression in irradiated rat liverInternational Journal of Radiation Oncology*Biology*Physics, 2000