HPLC Study on HPLC Characteristic Spectrum of Huoxiang (Herba Agastachis) Eliminating Summer-heat Soft Capsule

Abstract

To establish HPLC characteristic spectrum of Huoxiang (Herba Agastachis) Eliminating Summer-heat Soft Capsule. Chromatographic separation was performed on Tech Mate C18-ST (5 um, 4.6×250 nm) column by Agilent 1260 high performance liquid chromatograph, eluted with acetonitrile and 0.05% phosphoric acid in a gradient elution at a flow rate of 1.0 mL∙min-1. The detection wavelength was set at 270 nm. Hesperidin was selected as the reference peak to calculate the RSD of the relative retention time. Precision, stability and reproducibility were investigated as well. 80 different samples from 5 manufacturers were included in this study. The HPLC characteristic spectrum of Huoxiang Eliminating Summer-heat Soft Capsule was constructed with 11 specific chromatographic peaks in 80 samples. The method is accurate, reliable and with good reproducibility, and could overall control the quality of Huoxiang Eliminating Summerheat Soft Capsule.