Characterization of oestrogenic material basis of Cistanche deserticola total glycosides by ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry v1
Menopause is characterized by abnormal oestrogen levels. In our preliminary research, we identified that Cistanche deserticola exerts oestrogenic effects, and hypothesized it to be because of its glycoside constituents. In this study, we orally administered C. deserticola total glycosides (CDTG) daily at a dose of 1.5 g/kg body weight to sexually immature female Kunming mice. The negative and positive control groups received equal volumes of distilled water and diethylstilboestrol solution, respectively. On the fifth day, blood samples were collected, and the uteri were immediately excised and weighed. Proliferation of MCF7 cells was evaluated after they were exposed to the serum from each group of CDTG-treated mice. We used cluster analysis to distinguish the CDTG samples collected from 10 different sites. Further, 43 glycosides were identified in CDTG by UPLC/Q-TOF-MS. Finally, principal component, Greyrelativity, and bivariate correlation analysis were used to identify eight components that contributed to the oestrogenic activity of CDTG; they were sinapaldehyde glucoside, salidroside, syringin, dehydrodiconiferyl alcohol-4-O-β-D-glucoside, echinacoside, ononin, bartsioside, and kankanoside I. Among them, sinapaldehyde glucoside, syringin, dehydrodiconiferyl alcohol-4-O-β-D-glucoside, bartsioside and kankanoside I are first reported as oestrogenic active glycosides. Further studies are required to investigate the in vivo activity and safety of the identified components.