Tetrabutylammonium-chloride-glycerol of deep eutectic solvent functionalized MnO2: a novel mimic enzyme for the quantitative and qualitative colorimetric detection of l-cysteine
- 29 November 2022
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in The Analyst
- Vol. 148 (1), 182-190
- https://doi.org/10.1039/d2an01771g
Abstract
L-Cysteine is a common amino acid that plays an important role in human livelihood and production. Therefore, a novel method for the simultaneous quantitative and qualitative determination of L-cysteine by a colorimetric detection system is proposed. As a viable oxidase mimic, [N4444]Cl-G/MnO2, which consisted of MnO2 nanosheets functionalized by a tetrabutylammonium chloride–glycerol ([N4444]Cl-G) based deep eutectic solvent (DES) was fabricated. Owing to the oxidation of MnO2 nanosheets, [N4444]Cl-G/MnO2 could oxidize the colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into a blue product (oxTMB) with the characteristic UV–vis spectrum absorbance at 652 nm. The oxidation of TMB by DES/MnO2 was inhibited when L-cysteine was introduced, and the absorbance decreased proportionally with the increase in L-cysteine concentration. Due to this inhibition effect, a colorimetric detection system ([N4444]Cl-G/MnO2-TMB) was developed for the quantitative determination of L-cysteine. Under optimal conditions, the assay showed good linearity over the concentration range of 0.125–2.00 μg mL−1 with a low detection limit of 5.96 ng mL−1. A study of the inhibition mechanism demonstrated that the sulfhydryl group of L-cysteine could decompose [N4444]Cl-G/MnO2 into Mn2+, thus limiting the conversion of TMB to oxTMB. In addition, the [N4444]Cl-G/MnO2-TMB system was used in test strips for the visual qualitative detection of L-cysteine. The selectivity and test strip results demonstrated the high selectivity, simple operation, and rapid response of the [N4444]Cl-G/MnO2-TMB system for the qualitative detection of L-cysteine. Given the satisfying performance of the detection strategy, colorimetric sensing based on the [N4444]Cl-G/MnO2-TMB system is considered to have prospective application value in the quantitative and qualitative detection of L-cysteine.Funding Information
- National Natural Science Foundation of China (51864042, 22166031)
- Department of Education of Guizhou Province (QJJ[2022]092, QJJ[2022]003)
- Guizhou Science and Technology Department (QKHPTRC[2020]5009, QKHZC[2020]4Y043)
- Tongren University (trxyDH2107)
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