Regeneration of Pancreas Β-Cells in Alloxan-Induced Diabetes Rats Treated with Aqueous Seed Extract of Syzygium aromaticum: A Preliminary Study

Abstract

Aim: The present study was conducted to investigate the preventive/curative effect of aqueous seed extract Syzygium aromaticum in alloxan-induced diabetes in rats. Study Design: Twenty Wistar rats were randomly divided into four groups. The first group served as a control (received 1 ml of normal saline). The second group served as Diabetic control (received 1 ml of normal saline). The third group served as test 1 (pre-treated with 500 mg/kg body weight (b.w.) of aqueous Syzygium aromaticum seeds extract for seven days prior to diabetic induction and post-treated with aqueous seeds extract of Syzygium aromaticum after diabetic induction) and the fourth group served as test 2 (post-treated with 500 mg/kg b.w. of aqueous seed extract of Syzygium aromaticum after diabetic induction) in a daily oral dose for 14 days. Results: Treatment of rats with aqueous seed extract of Syzygium aromaticum in a daily dose of 500 mg/kg significantly mitigates the induced changes in the glucose and lipid profile parameters. Histopathological examination of the pancreas showed the destruction of β-cells in the diabetic control group and recovery of damaged tissues when treated with 500 mg/kg b.w. of aqueous seeds extract of Syzygium aromaticum. Conclusion: The present study suggests that aqueous seed extract S. aromaticum seeds extract at a dose of 500 mg/kg body weight brings about regeneration of the B-cells of the pancreas and significant beneficial effects in various physiological parameters in alloxan-induced diabetes.