Recovery of platelet‐rich red blood cells and acquisition of convalescent plasma with a novel gravity‐driven blood separation device
Open Access
- 10 November 2021
- journal article
- research article
- Published by Wiley in Transfusion Medicine
- Vol. 32 (1), 53-63
- https://doi.org/10.1111/tme.12830
Abstract
Objectives Our objectives were to determine the separation characteristics and blood product quality of a gravity-driven microfiltration blood separation system (HemoClear, The Netherlands). Background A range of centrifugal blood separation devices, including intraoperative cell salvage devices (cell savers) and apheresis machines, are available to assist in preparing both allogenic and autologous blood products. These devices are expensive to operate and require extensive training. Methods and Materials Nine whole blood units were collected under standard conditions and analysed for haematological parameters, thromboelastographic properties, platelet morphology and activation, and red blood cell (RBC) deformability and morphology. Three whole blood units were separated by means of the HemoClear device, into a liquid and cellular component. The cellular component was diluted with SAGM and cold stored for 14 days. To simulate cell salvage six whole blood units were diluted with isotonic saline, followed by multiple HemoClear separation rounds. Results The recovery of both RBCs (100 ± 1.6%) and white blood cells (99 ± 4.5%) after undiluted filtration were very high, while platelet recovery was high (83 ± 3.0%). During the filtration, and cold storage after filtration storage both the non-deformable RBC fraction and the RBC maximum elongation remained stable. Parameters of thromboelastography indicated that platelets remain functional after filtration and after 7 days of cold storage. In the cell salvage simulation the total protein load in the cellular fraction was reduced by 65 ± 4.1% after one washing round and 84 ± 1.9% after two consecutive washing rounds. Conclusion The novel blood filter studied effectively separates whole blood into diluted plasma and platelet-rich RBCs. Moreover, the device effectively washed diluted whole blood, driving over 80% of proteins to the liquid component.Keywords
This publication has 31 references indexed in Scilit:
- Hemolysis of red blood cells after cell washing with different automated technologies: clinical implications in a neonatal cardiac surgery populationTransfusion, 2010
- Normal range values for thromboelastography in healthy adult volunteersBrazilian Journal of Medical and Biological Research, 2009
- Red cell hemolysis during processing and storageAsian Journal of Transfusion Science, 2007
- Blood component collection by apheresisJournal of Clinical Apheresis, 2006
- Measurement of the distribution of red blood cell deformability using an automated rheoscopeCytometry, 2002
- Rheological properties and function of blood cells in stored bank blood and salvaged bloodBritish Journal of Haematology, 1998
- Detection of platelet activation with monoclonal antibodies and flow cytometry. Changes during platelet storageTransfusion, 1990
- Storage of Whole Blood for up to 24 Hours at Ambient Temperature prior to Component PreparationVox Sanguinis, 1989
- A Study of Variables Affecting the Quality of Platelets Stored at “Room Temperature”Transfusion, 1975
- Morphology of Stored, Rejuvenated Human ErythrocytesVox Sanguinis, 1975