Anti- SARS-CoV-2 Receptor Binding Domain Antibody Evolution after mRNA Vaccination
Preprint
- 29 July 2021
- preprint
- research article
- Published by Cold Spring Harbor Laboratory
Abstract
Summary: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection produces B-cell responses that continue to evolve for at least one year. During that time, memory B cells express increasingly broad and potent antibodies that are resistant to mutations found in variants of concern1. As a result, vaccination of coronavirus disease 2019 (COVID-19) convalescent individuals with currently available mRNA vaccines produces high levels of plasma neutralizing activity against all variants tested1, 2. Here, we examine memory B cell evolution 5 months after vaccination with either Moderna (mRNA-1273) or Pfizer- BioNTech (BNT162b2) mRNA vaccines in a cohort of SARS-CoV-2 naïve individuals. Between prime and boost, memory B cells produce antibodies that evolve increased neutralizing activity, but there is no further increase in potency or breadth thereafter. Instead, memory B cells that emerge 5 months after vaccination of naïve individuals express antibodies that are similar to those that dominate the initial response. While individual memory antibodies selected over time by natural infection have greater potency and breadth than antibodies elicited by vaccination, the overall neutralizing potency of plasma is greater following vaccination. These results suggest that boosting vaccinated individuals with currently available mRNA vaccines will increase plasma neutralizing activity but may not produce antibodies with breadth equivalent to those obtained by vaccinating convalescent individuals.Keywords
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This publication has 62 references indexed in Scilit:
- Expression kinetics of nucleoside-modified mRNA delivered in lipid nanoparticles to mice by various routesJournal of Controlled Release, 2015
- Change-O: a toolkit for analyzing large-scale B cell immunoglobulin repertoire sequencing dataBioinformatics, 2015
- Apoptosis and antigen affinity limit effector cell differentiation of a single naïve B cellScience, 2015
- Class-switched memory B cells remodel BCRs within secondary germinal centersNature Immunology, 2015
- Pandemic H1N1 influenza vaccine induces a recall response in humans that favors broadly cross-reactive memory B cellsProceedings of the National Academy of Sciences of the United States of America, 2012
- Germinal CentersAnnual Review of Immunology, 2012
- Rapid and Massive Virus-Specific Plasmablast Responses during Acute Dengue Virus Infection in HumansJournal of Virology, 2012
- Different B Cell Populations Mediate Early and Late Memory During an Endogenous Immune ResponseScience, 2011
- Rapid cloning of high-affinity human monoclonal antibodies against influenza virusNature, 2008
- Duration of Humoral Immunity to Common Viral and Vaccine AntigensThe New England Journal of Medicine, 2007