Preparation andin vitrocharacterization of vascular endothelial growth factor (VEGF)-loaded poly(D,L-lactic-co-glycolic acid) microspheres using a double emulsion/solvent evaporation technique
- 14 October 2010
- journal article
- research article
- Published by Taylor & Francis Ltd in Journal of Microencapsulation
- Vol. 28 (1), 46-54
- https://doi.org/10.3109/02652048.2010.523795
Abstract
Biodegradable Poly(lactic-co-glycolic acid; PLGA), microspheres encapsulating the angiogenic protein recombinant human vascular endothelial growth factor (rhVEGF) were formed to achieve VEGF release in a sustained manner. These microspheres are a promising delivery system which can be used for therapeutic angiogenesis. The PLGA microspheres incorporating two different initial loading amounts of rhVEGF have been prepared by a modified water-in-oil-in-water (w/o/w) double emulsion/solvent evaporation technique. The microspheres have been characterized by particle size distribution, environmental scanning electron microscopy (ESEM), light microscopy, encapsulation efficiency and their degradation was studied in vitro. The rhVEGF released from microspheres was quantified by the competitive enzyme-linked immunosorbent assay (ELISA) and human umbilical vein endothelial cell (HUVEC) proliferation assay was used to assess biological activity of the released VEGF. The microspheres were spherical with diameters of 10–60 µm and the encapsulation efficiency was between 46% and 60%. The release kinetics of rhVEGF was studied for two different amounts: 5 µg VEGF (V5) and 50 µg VEGF (V50) per 500 mg starting polymer. The total protein (VEGF:BSA) release increased up to 4 weeks for two rhVEGF concentrations. The ELISA results showed that the burst release for V5 and V50 microspheres were 4 and 27 ng/mL, respectively. For V5, the microspheres showed an initial burst release, followed by a higher steady-state release until 14 days. VEGF release increased up to 2 weeks for V50 microsphere. HUVEC proliferation assay showed that endothelial cells responded to bioactive VEGF by proliferating and migrating.Keywords
This publication has 37 references indexed in Scilit:
- Vascular endothelial growth factor receptor-2: Structure, function, intracellular signalling and therapeutic inhibitionCellular Signalling, 2007
- Surface engineered and drug releasing pre-fabricated scaffolds for tissue engineeringAdvanced Drug Delivery Reviews, 2007
- AngiogenesisAnnual Review of Medicine, 2006
- Microencapsulation peptide and protein drugs delivery systemColloids and Surfaces B: Biointerfaces, 2005
- Development andin vitro characterization of vascular endothelial growth factor (VEGF)-loaded poly(DL-lactic-co-glycolic acid)/poly(ethylene glycol) microspheres using a solid encapsulation/single emulsion/solvent extraction techniqueJournal of Biomedical Materials Research, 2000
- Protein stability in controlled-release systemsNature Biotechnology, 2000
- Protein delivery from poly(lactic-co-glycolic acid) biodegradable microspheres: Release kinetics and stability issuesJournal of Microencapsulation, 1998
- Effect of solvent removal technique on the matrix characteristics of polylactide/glycolide microspheres for peptide deliveryJournal of Controlled Release, 1996
- Sterilization, toxicity, biocompatibility and clinical applications of polylactic acid/ polyglycolic acid copolymersBiomaterials, 1996
- Development of poly(ortho esters): a historical overviewBiomaterials, 1990