The purpose of this research was to depict the profile,to reveal the practice of dhikr and prayerof the tarekat, to describe various religious and social activities of Jam'iyah Khalwatiyah Syekh Yusuf al-Makassariy.The research method was field research conducting in Makassar City, Pangkep Regency, Maros Regency and Gowa Regency. The approach was mainly the historical and theological approach. The data source was the murshid and tarekat Khalwatiyah Syekh Yusuf al-Makassariy. The profile of Khalwatiyah Syekh Yusuf al-Makassarycan be seen from two points of view: as jam'iyah and as a tarekat school. Jam'iyah is an association or rather as a forum, an organization gathering followers of the tarekat Khalwatiyah Syekh Yusuf al-Makassariy. The practice of dhikrfor the tarekat worshipers is always reciting لاَاَلٰهَ اِلاَّ اللهُ (La Ilaha Illallah). In addition to dhikris prayer and the prayer before and after waking up are praticed most of the time. Furthermore, the worship practices in fard and sunnah prayer are given priority, particularly the Lail and Tahajjud prayer in the middle of the night and in the morning is the Dhuha prayer. For social practice, especially in Eid Al-Fitr, the Jam'iyah distributed zakat fitrah and in Eid al-Adha, distributing sacrificial meat and basic necessities are distributed to the poor.