BACTERIOLOGICAL CHARACTERISTICS OF DIABETIC FOOT INFECTION AND SUSCEPTIBILITY OF MULTIRESISTANT ISOLATES TO HYDRAULIC EXTRACTS FROM ALLIUM SATIVA, ALLIUM CEPA AND CANNABIS SATIVA
Background and objective : Diabetic foot infection is one of the most serious complications of diabetes and its persistence is the result of the ineffectiveness of antibiotic therapy due to the exponentially increasing of antibiotic resistant bacteria. The study aimed at investigating the antibacterial effect of the aqueous extract of some plants on the antibiotic resistant bacteria isolated from diabetic foot wound infections. Methods : A 6-months cross-sectional study from July 2021 to January 2022 at the Yaoundé Central Hospital, was undertaken with diabetic foot wound patients. All samples were appraised to determine presence of infectious agents using standard methods for isolation and identification of bacteria. Subsequently, Antibiotic resistance was done using Kirby-Bauer disc diffusion methods. Finally, extracts of Cannabis sativa leaves, Allium cepa and Allium sativum bulbs were obtained with water and their antibacterial activities were evaluated by the microdillution method on liquid medium. Results : 20 patients whom 14 men were included, with a sex ratio of 2.33, and their mean age was 52.5±9.6 years. 60% of these patients presented wounds in grade III and were of several types : purulent (48.57%), moist (31.43%) and dry (20%). 35 strains were isolated. The predominant GPB were S. aureus (34.29%) followed by Coagulase-negative Staphylococcus (14.29%), and Bacillus Spp (2.86%). Among the GNB, Pseudomonas aeruginosa (11.46%), Serratia Spp. (8.56%), Escherichia coli (8.56%), Enterobacter Spp. (5.71%), Proteus Spp. (5.71%), Klebsiella Spp., Yersinia Spp. and Salmonella Spp. in proportions of 2.86% each. A high rate of antibiotic resistance was recorded for Oxacillin (100%), Vancomycin (83.34%) and Augmentin (55.56%). Sensitivity tests on liquid medium showed that MIC ranged between 3.12-25.00 mg/mL, 6.25-25.00 mg/mL and 1.86-25.00 mg/mL respectivelly for A. sativa, A. cepa and C. sativa. Alliums were much more active on GNB. Although these results are low, they could be an alternative for the diabetic foot infection treatment. Conclusion : Alliums were much more active on GNB Although these results are low, they could be an alternative for the diabetic foot infection treatment. Peer Review History: Received: 28 February 2022; Revised: 15 April; Accepted: 24 April, Available online: 15 May 2022 Academic Editor: Dr. Sally A. El-Zahaby, Pharos University in Alexandria, Egypt, sally.elzahaby@yahoo.com UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency. Received file: Reviewer's Comments: Average Peer review marks at initial stage: 5.5/10 Average Peer review marks at publication stage: 7.0/10 Reviewers: Dr. Sangeetha Arullappan, Universiti Tunku Abdul Rahman, Malaysia, sangeetha@utar.edu.myProf. Dr. Ali Gamal Ahmed Al-kaf, Sana'a university, Yemen, alialkaf21@gmail.com Similar Articles: THE SCOPING REVIEW OF CHINESE AND WESTERN MEDICINE TREATMENT OF DIABETIC FOOT IN ASIA A SURVEY ABOUT THE CURRENT SCENARIO OF DIABETES IN CHATTOGRAM AREA BY DETERMINING PRACTICE OF THE SELF-CARE ACTIVITIES AMONGST PEOPLE WITH DIABETES
