Automatic Spot Identification Method for High Throughput Surface Plasmon Resonance Imaging Analysis
Open Access
- 13 September 2018
- journal article
- research article
- Published by MDPI AG in Biosensors
- Vol. 8 (3), 85
- https://doi.org/10.3390/bios8030085
Abstract
An automatic spot identification method is developed for high throughput surface plasmon resonance imaging (SPRi) analysis. As a combination of video accessing, image enhancement, image processing and parallel processing techniques, the method can identify the spots in SPRi images of the microarray from SPRi video data. In demonstrations of the method, SPRi video data of different protein microarrays were processed by the method. Results show that our method can locate spots in the microarray accurately regardless of the microarray pattern, spot-background contrast, light nonuniformity and spotting defects, but also can provide address information of the spots.Keywords
This publication has 31 references indexed in Scilit:
- An Image Sharpening Method by Suppressing the NoiseInternational Journal of Computer Applications, 2012
- Data Analysis Strategies for Protein MicroarraysBioTech, 2012
- An adaptive local binarization method for document images based on a novel thresholding method and dynamic windowsPattern Recognition Letters, 2011
- Surface plasmon resonance biosensor for parallelized detection of protein biomarkers in diluted blood plasmaBiosensors and Bioelectronics, 2010
- A small-molecule inhibitor shows that pirin regulates migration of melanoma cellsNature Chemical Biology, 2010
- Quantitative Serum Proteomics from Surface Plasmon Resonance ImagingMolecular & Cellular Proteomics, 2008
- Surface Plasmon Resonance Sensors for Detection of Chemical and Biological SpeciesChemical Reviews, 2008
- SPR microscopy and its applications to high-throughput analyses of biomolecular binding events and their kineticsBiomaterials, 2007
- A Robust Small-Molecule Microarray Platform for Screening Cell LysatesCell Chemical Biology, 2006
- SPR Imaging of Photo-Cross-Linked Small-Molecule Arrays on GoldAnalytical Chemistry, 2006