Taking 13 prefecture-level cities in Jiangsu Province as the research object, based on the theory of spatial dislocation, the gravity model and two-dimensional composite matrix method are used to analyze the spatial dislocation between tourism resources, permanent population and other factors in Jiangsu Province and tourism revenue. The results show that the population center, the center of tourism resources, the center of tourist numbers, the center of economic development, and the center of tourism revenue of Jiangsu Province are all biased toward the southern part of Jiangsu Province. From the analysis of four sets of two-dimensional composite matrixes, 13 prefecture-level cities have synchronized coordination and also have positive and negative dislocation types. Southern Jiangsu has the best synchronization and coordination, and northern Jiangsu has a strong negative dislocation trend; the combination of tourist numbers and tourism revenue is the strongest, but the dislocation is weak; population and tourism revenue have a strong positive dislocation trend; there is a strong negative dislocation trend between economic development and tourism revenue; the matrix combination of Nanjing, Wuxi and Suzhou has good synchronization; the city of Xuzhou in northern Jiangsu has a strong negative dislocation. In view of the results of spatial dislocation analysis, suggestions for improvement and optimization are put forward to promote the high-quality development of tourism in Jiangsu Province.