Whitefly is a serious threat to the cotton production. To find out the most suitable control measure a field experiment was conducted at District Layyah, to compare the efficacy of different pre-tank mix insecticides., viz. Legand (spiromesifen + abamectin), Actify (Isoprocarb + etiprole), Bila (Pyriproxyfen + acetamaprid), Best Guard (buprofezin + nitenpyram), Jeera (Pyriproxyfen + acetamaprid), Rugra (nitenpyram + chlorfenapyr), Editor (acetamaprid + ibiocydam), and Concept Plus (acephate + phenoxaprop) on BT-886 and Non-BT (Shahkar) cotton varieties. Maximum population reduction of adult whitefly after first application of insecticides was found in Legand on BT and Non-BT cotton crop exhibited, 24.37 and 37.66 after 2 days, 53.08 and 55.31 after 4 days and 73.29 and 68.17 after 6 days respectively. Maximum population reduction of nymph whitefly was found in Legand on BT and Non-BT cotton crop exhibited 33.85 and 34.07 after 2 days, 55.10 and 54.49 after 4 days and 74.66 and 75.28 after 6 days respectively. Maximum population reduction of adult whitefly after second application of insecticides was found in Legand on BT and Non-BT cotton crop exhibited, 24.56 and 39.57 after 2 days, 30.29 and 55.97 after 4 days and 71.34 and 69.62 4after 6 days respectively. Maximum population reduction of nymph whitefly was found in Legand on BT and Non-BT cotton crop exhibited 34.07 and 40.52 after 2 days, 54.49, after 4 days and 75.28 and 74.92 after 6 days respectively.