The Anti-oxidant and Anti-inflammatory Properties of Cerium Oxide Nanoparticles Synthesized Using Origanum majorana L. Leaf Extract
Open Access
- 30 September 2019
- journal article
- Published by Maad Rayan Publishing Company in International Journal of Basic Science in Medicine
- Vol. 4 (3), 108-112
- https://doi.org/10.15171/ijbsm.2019.20
Abstract
Introduction: Free radicals have singlet electron in their outer layer rendering them high reactivity against biomolecules (i.e., DNA, carbohydrates, proteins, and lipids). Oxidative stress is created when the production of free radicals exceeds their removal by antioxidant systems and is involved in the pathogenesis of several diseases such as diabetes, arthritis, inflammatory conditions, and various cancers. Regarding the therapeutic potential of nanoparticles (NPs) in human diseases, the purpose of this study was to synthesize cerium oxide NPs using Origanum majorana leaf extract. Methods: Cerium oxide nanoparticles (CeO2 -NPs) were synthesized using aqueous leaf extract of O. majorana. The sizes of NPs were characterized by a particle size analyzer. The antioxidant properties of the CeO2 -NPs were determined by Ferric-reducing antioxidant power (FRAP) assay. The anti-inflammatory effects of the NPs were also determined by measuring gene expressions of IL-1β and IL-10 using real-time polymerase chain reaction (PCR). Results: The CeO2 -NPs were successfully synthesized using O. majorana leaf extract. The results of FRAP assay showed that the anti-oxidant activities of CeO2 -NPs at concentrations of 50, 100, and 400 μg/mL were 75%, 77.1%, and 94.5%, respectively. Moreover, interleukin 10 (IL-10) gene expressions increased by 4.6 folds while the expression of IL-1β gene decreased by 0.75-fold in HUVECs. Conclusion: The CeO2 -NPs synthesized using the aqueous extract of O. majorana demonstrated antioxidant and anti-inflammatory properties. Therefore, these NPs can be used as potential therapeutic agents in medicine.Keywords
This publication has 28 references indexed in Scilit:
- Cerium Oxide Nanoparticles Inhibits Oxidative Stress and Nuclear Factor-κB Activation in H9c2 Cardiomyocytes Exposed to Cigarette Smoke ExtractThe Journal of pharmacology and experimental therapeutics, 2011
- Pharmacological potential of cerium oxide nanoparticlesNanoscale, 2011
- A biotechnological perspective on the application of iron oxide magnetic colloids modified with polysaccharidesBiotechnology Advances, 2011
- Metal Oxide Nanoparticles Induce Unique Inflammatory Footprints in the Lung: Important Implications for Nanoparticle TestingEnvironmental Health Perspectives, 2010
- Supercritical fluid extraction of oregano (Origanum vulgare) essentials oils: Anti-inflammatory properties based on cytokine response on THP-1 macrophagesFood and Chemical Toxicology, 2010
- Free radicals, antioxidants and functional foods: Impact on human healthPharmacognosy Reviews, 2010
- Anti‐inflammatory Properties of Cerium Oxide NanoparticlesSmall, 2009
- Structure-sensitive properties and biomedical applications of nanodispersed cerium dioxideRussian Chemical Reviews, 2009
- Free radicals, metals and antioxidants in oxidative stress-induced cancerChemico-Biological Interactions, 2006
- Modified 2,2-Azino-bis-3-ethylbenzothiazoline-6-sulfonic Acid (ABTS) Method to Measure Antioxidant Capacity of Selected Small Fruits and Comparison to Ferric Reducing Antioxidant Power (FRAP) and 2,2‘-Diphenyl-1-picrylhydrazyl (DPPH) MethodsJournal of Agricultural and Food Chemistry, 2006