Single-cell RNA sequencing for the identification of early-stage lung cancer biomarkers from circulating blood
Open Access
- 15 October 2021
- journal article
- research article
- Published by Springer Science and Business Media LLC in npj Genomic Medicine
- Vol. 6 (1), 1-15
- https://doi.org/10.1038/s41525-021-00248-y
Abstract
Lung cancer accounts for more than half of the new cancers diagnosed world-wide with poor survival rates. Despite the development of chemical, radiological, and immunotherapies, many patients do not benefit from these therapies, as recurrence is common. We performed single-cell RNA-sequencing (scRNA-seq) analysis using Fluidigm C1 systems to characterize human lung cancer transcriptomes at single-cell resolution. Validation of scRNA-seq differentially expressed genes (DEGs) through quantitative real time-polymerase chain reaction (qRT-PCR) found a positive correlation in fold-change values between C-X-C motif chemokine ligand 1 (CXCL1) and 2 (CXCL2) compared with bulk-cell level in 34 primary lung adenocarcinomas (LUADs) from Stage I patients. Furthermore, we discovered an inverse correlation between chemokine mRNAs, miR-532-5p, and miR-1266-3p in early-stage primary LUADs. Specially, miR-532-5p was quantifiable in plasma from the corresponding LUADs. Collectively, we identified markers of early-stage lung cancer that were validated in primary lung tumors and circulating blood.Keywords
This publication has 43 references indexed in Scilit:
- The T-box transcription factor Brachyury promotes epithelial-mesenchymal transition in human tumor cellsJCI Insight, 2010
- A Gene Expression Signature Associated with “K-Ras Addiction” Reveals Regulators of EMT and Tumor Cell SurvivalCancer Cell, 2009
- A gene-alteration profile of human lung cancer cell linesHuman Mutation, 2009
- Frequent deletion of the CDKN2A locus in chordoma: analysis of chromosomal imbalances using array comparative genomic hybridisationBritish Journal of Cancer, 2007
- Brachyury, a crucial regulator of notochordal development, is a novel biomarker for chordomasThe Journal of Pathology, 2006
- EGFRMutation and Resistance of Non–Small-Cell Lung Cancer to GefitinibThe New England Journal of Medicine, 2005
- Involvement of PI3K/Akt pathway in cell cycle progression, apoptosis, and neoplastic transformation: a target for cancer chemotherapyLeukemia, 2003
- Targeting RAS signalling pathways in cancer therapyNature Reviews Cancer, 2003
- A new mathematical model for relative quantification in real-time RT-PCRNucleic Acids Research, 2001
- Characterization of the cyclin-dependent kinase inhibitory domain of the INK4 family as a model for a synthetic tumour suppressor moleculeOncogene, 1998