Mobilization of endothelial precursor cells: Systemic vascular response to musculoskeletal trauma
- 25 September 2006
- journal article
- Published by Wiley in Journal of Orthopaedic Research
- Vol. 25 (1), 44-50
- https://doi.org/10.1002/jor.20228
Abstract
Postnatal vasculogenesis, the process by which vascular committed bone marrow stem cells or endothelial precursor cells (EPC) migrate, differentiate, and incorporate into the nacent endothelium contributing to physiological and pathological neovascularization, has stimulated much interest. Its contribution to tumor nonvascularization, wound healing, and revascularization associated with skeletal and cardiac muscles ischaemia is established. We evaluated the mobilization of EPCs in response to musculoskeletal trauma. Blood from patients (n = 15) following AO type 42a1 closed diaphyseal tibial fractures was analyzed for CD34 and AC133 cell surface marker expression. Immunomagnetically enriched CD34+ mononuclear cell (MNCCD34+) populations were cultured and examined for phenotypic and functional vascular endothelial differentiation. Circulating MNCCD34+ levels increased sevenfold by day 3 postinjury. Circulating MNCAC133+ increased 2.5‐fold. Enriched MNCCD34+ populations from day 3 samples in culture exhibited cell cluster formation with sprouting spindles. These cells bound UEA‐1 and incorporated fluorescent DiI‐Ac‐LDL intracellularily. Our findings suggest a systemic provascular response is initiated in response to musculoskeletal trauma. Its therapeutic manipulation may have implications for the potential enhancement of fracture healing. © 2006 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 25:44–50, 2007This publication has 38 references indexed in Scilit:
- Cellular basis for age-related changes in fracture repair.Journal of Orthopaedic Research, 2005
- Delayed Arteriogenesis in Hypercholesterolemic MiceJournal of the American College of Cardiology, 2005
- Adenoviral VEGF‐A gene transfer induces angiogenesis and promotes bone formation in healing osseous tissuesThe Journal of Gene Medicine, 2003
- The effects of blood glucose control upon fracture healing in the BB Wistar rat with diabetes mellitusJournal of Orthopaedic Research, 2002
- Purification and ex vivo expansion of postnatal human marrow mesodermal progenitor cellsBlood, 2001
- Blood-derived angioblasts accelerate blood-flow restoration in diabetic miceJCI Insight, 2000
- Transplanted cord blood–derived endothelial precursor cells augment postnatal neovascularizationJCI Insight, 2000
- Origins of circulating endothelial cells and endothelial outgrowth from bloodJCI Insight, 2000
- Heterozygous embryonic lethality induced by targeted inactivation of the VEGF geneNature, 1996
- Failure of blood-island formation and vasculogenesis in Flk-1-deficient miceNature, 1995