Label-free Photoelectrochemical Aptasensor for the Determination of Carcinoembryonic Antigen Using a Cadmum Sulfide Quantum Dot Sensitized Titanium (IV) Oxide Nanotube Electrode
- 14 December 2017
- journal article
- research article
- Published by Taylor & Francis Ltd in Analytical Letters
- Vol. 51 (6), 820-833
- https://doi.org/10.1080/00032719.2017.1360900
Abstract
A label-free photoelectrochemical aptasensor for the sensitive and selective determination of carcinoembryonic antigen was constructed based on a CdS quantum dot sensitized TiO2 nanotube electrode. TiO2 nanotubes with highly ordered structure and more active sites than bulk TiO2 were prepared with an electrochemical anodic oxidation process. The CdS quantum dots were immobilized on the TiO2 nanotubes using poly(diallyldimethylammonium chloride) as a bridge. Due to the energy level match between TiO2 and CdS, the CdS quantum dots/TiO2 nanotubes electrode exhibits excellent photoelectrochemical performance. The large surface area of the electrode also allows for capturing large numbers of aptamers. The fine photoelectrochemical performance and the large surface area of the electrode greatly enhanced the detection sensitivity. Under the optimal conditions, the prepared photoelectrochemical aptasensor presents desirable analytical properties for the determination of carcinoembryonic antigen in the range of 0.05 to 10 ng mL−1 with a detection limit of 0.014 ng mL−1. The application of the designed protocol was investigated by analyzing carcinoembryonic antigen in human serum samples with recoveries from 80.0 to 115.0%. This simple and sensitive method provides an alternative tool to standard biochemical assays.Keywords
Funding Information
- The National Natural Science Foundation of China (21675136, 21375114, 21405129)
- Plan for Scientific Innovation Talent of Henan Province
- the Young Backbone Teachers of Higher Education Institutions in Henan Province
- Major Projects of Science and Technology of Henan
- Xinyang Normal University
- Nanhu Young Scholar Supporting Program of XYNU
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