The type 3 secretion system requires actin polymerization to open translocon pores
Open Access
- 9 September 2021
- journal article
- research article
- Published by Public Library of Science (PLoS) in PLoS Pathogens
- Vol. 17 (9), e1009932
- https://doi.org/10.1371/journal.ppat.1009932
Abstract
Many bacterial pathogens require a type 3 secretion system (T3SS) to establish a niche. Host contact activates bacterial T3SS assembly of a translocon pore in the host plasma membrane. Following pore formation, the T3SS docks onto the translocon pore. Docking establishes a continuous passage that enables the translocation of virulence proteins, effectors, into the host cytosol. Here we investigate the contribution of actin polymerization to T3SS-mediated translocation. Using the T3SS model organism Shigella flexneri, we show that actin polymerization is required for assembling the translocon pore in an open conformation, thereby enabling effector translocation. Opening of the pore channel is associated with a conformational change to the pore, which is dependent upon actin polymerization and a coiled-coil domain in the pore protein IpaC. Analysis of an IpaC mutant that is defective in ruffle formation shows that actin polymerization-dependent pore opening is distinct from the previously described actin polymerization-dependent ruffles that are required for bacterial internalization. Moreover, actin polymerization is not required for other pore functions, including docking or pore protein insertion into the plasma membrane. Thus, activation of the T3SS is a multilayered process in which host signals are sensed by the translocon pore leading to the activation of effector translocation. The type 3 secretion system (T3SS) is required for the virulence of a variety of bacteria that infect humans. The T3SS forms a pore in the host cell membrane that is a conduit for delivering virulence proteins into the cell. Here, we demonstrate that actin polymerization is necessary to convert T3SS pores into an open conformation that is competent for virulence protein delivery. We find that activation of type 3 secretion proceeds in a multistep process whereby bacteria dock onto the translocon pore and then activate secretion and delivery of virulence proteins.Keywords
Funding Information
- National Institute of Allergy and Infectious Diseases (R01AI081724)
- National Institute of Allergy and Infectious Diseases (F32AI147549)
- National Institute of Allergy and Infectious Diseases (T32AI007061)
- National Institute of Allergy and Infectious Diseases (K22AI137296)
- National Institute of Allergy and Infectious Diseases (F32AI114162)
- Executive Committee on Research, Massachusetts General Hospital
- Charles A. King Trust
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